SitesBLAST
Comparing WP_037570960.1 NCBI__GCF_000744815.1:WP_037570960.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
72% identity, 93% coverage: 20:273/274 of query aligns to 4:257/258 of 4wecA
- active site: G21 (= G37), S143 (= S159), Q154 (= Q170), Y157 (= Y173), K161 (= K177)
- binding nicotinamide-adenine-dinucleotide: G17 (= G33), A19 (≠ G35), S20 (≠ G36), G21 (= G37), I22 (= I38), D41 (= D57), I42 (≠ V58), V61 (≠ T77), D62 (= D78), V63 (= V79), N89 (= N105), T141 (= T157), Y157 (= Y173), K161 (= K177), P187 (= P203), P189 (= P205), V190 (= V206)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
41% identity, 89% coverage: 23:266/274 of query aligns to 4:242/244 of 4nbuB
- active site: G18 (= G37), N111 (= N131), S139 (= S159), Q149 (= Q170), Y152 (= Y173), K156 (= K177)
- binding acetoacetyl-coenzyme a: D93 (= D114), K98 (vs. gap), S139 (= S159), N146 (≠ A167), V147 (≠ T168), Q149 (= Q170), Y152 (= Y173), F184 (≠ P205), M189 (≠ L210), K200 (≠ R225)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G33), N17 (≠ G36), G18 (= G37), I19 (= I38), D38 (= D57), F39 (≠ V58), V59 (≠ T77), D60 (= D78), V61 (= V79), N87 (= N105), A88 (= A106), G89 (= G107), I90 (= I108), T137 (= T157), S139 (= S159), Y152 (= Y173), K156 (= K177), P182 (= P203), F184 (≠ P205), T185 (≠ V206), T187 (= T208), M189 (≠ L210)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
38% identity, 89% coverage: 24:267/274 of query aligns to 3:246/248 of 6ixmC
- active site: G16 (= G37), S142 (= S159), Y155 (= Y173), K159 (= K177)
- binding nicotinamide-adenine-dinucleotide: G12 (= G33), S15 (≠ G36), G16 (= G37), I17 (= I38), D36 (= D57), I37 (≠ V58), A61 (≠ T77), D62 (= D78), T63 (≠ V79), N89 (= N105), A90 (= A106), M140 (≠ T157), S142 (= S159), Y155 (= Y173), K159 (= K177), P185 (= P203), A186 (≠ G204), Y187 (≠ P205), I188 (≠ V206), L192 (= L210)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
42% identity, 91% coverage: 23:270/274 of query aligns to 10:267/267 of Q9LBG2
- 17:42 (vs. 30:55, 65% identical) binding NAD(+)
- E103 (≠ S109) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
1iy8A Crystal structure of levodione reductase (see paper)
42% identity, 91% coverage: 23:270/274 of query aligns to 1:258/258 of 1iy8A
- active site: G15 (= G37), S143 (= S159), Q153 (= Q170), Y156 (= Y173), K160 (= K177)
- binding nicotinamide-adenine-dinucleotide: G11 (= G33), S14 (≠ G36), G15 (= G37), L16 (≠ I38), D35 (= D57), V36 (= V58), A62 (≠ T77), D63 (= D78), V64 (= V79), N90 (= N105), G92 (= G107), I93 (= I108), T141 (= T157), S143 (= S159), Y156 (= Y173), K160 (= K177), P186 (= P203), G187 (= G204), T191 (= T208), P192 (= P209), M193 (≠ L210)
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme
37% identity, 90% coverage: 22:267/274 of query aligns to 2:247/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (= G33), S16 (≠ G36), G17 (= G37), I18 (= I38), D37 (= D57), I38 (≠ V58), A62 (≠ T77), D63 (= D78), S64 (≠ V79), N90 (= N105), M141 (≠ T157), Y156 (= Y173), K160 (= K177), P186 (= P203), G187 (= G204), Y188 (≠ P205), I189 (≠ V206), L193 (= L210)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
43% identity, 89% coverage: 22:265/274 of query aligns to 1:247/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G33), G16 (= G37), I17 (= I38), D36 (= D57), L37 (≠ V58), C61 (≠ T77), D62 (= D78), V63 (= V79), N89 (= N105), A90 (= A106), T140 (= T157), S142 (= S159), Y155 (= Y173), K159 (= K177), A186 (≠ G204), V187 (≠ P205)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
40% identity, 89% coverage: 23:266/274 of query aligns to 5:245/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G33), R18 (≠ G36), G19 (= G37), I20 (= I38), D39 (= D57), R40 (≠ V58), C63 (≠ T77), I65 (≠ V79), N91 (= N105), G93 (= G107), I94 (= I108), V114 (= V130), Y155 (= Y173), K159 (= K177), I188 (≠ V206), T190 (= T208), T193 (≠ L211)
2cfcA Structural basis for stereo selectivity in the (r)- and (s)- hydroxypropylethane thiosulfonate dehydrogenases (see paper)
39% identity, 88% coverage: 25:264/274 of query aligns to 1:245/250 of 2cfcA
- active site: G13 (= G37), S142 (= S159), Y155 (= Y173), K159 (= K177)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ A167), R152 (≠ Q170), Y155 (= Y173), W195 (≠ E213), R196 (≠ L214)
- binding nicotinamide-adenine-dinucleotide: G9 (= G33), S12 (≠ G36), G13 (= G37), N14 (≠ I38), D33 (= D57), L34 (≠ V58), A59 (≠ T77), D60 (= D78), V61 (= V79), N87 (= N105), A88 (= A106), G89 (= G107), I140 (≠ T157), P185 (= P203), G186 (= G204), M187 (≠ P205), I188 (≠ V206), T190 (= T208), P191 (= P209), M192 (≠ L210), T193 (≠ L211)
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
39% identity, 88% coverage: 25:264/274 of query aligns to 1:245/250 of Q56840
- M1 (≠ V25) modified: Initiator methionine, Removed
- SGN 12:14 (≠ GGI 36:38) binding NAD(+)
- D33 (= D57) binding NAD(+)
- DV 60:61 (= DV 78:79) binding NAD(+)
- N87 (= N105) binding NAD(+)
- S142 (= S159) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (≠ Q170) binding 2-oxopropyl-coenzyme M; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y173) mutation Y->E,F: Loss of activity.
- K159 (= K177) mutation to A: Loss of activity.
- R179 (= R197) mutation to A: Loss of activity.
- IETPM 188:192 (≠ VDTPL 206:210) binding NAD(+)
- WR 195:196 (≠ EL 213:214) binding 2-oxopropyl-coenzyme M
- R196 (≠ L214) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- R203 (≠ A222) mutation to A: Slight decrease in catalytic efficiency.
- R209 (≠ H228) mutation to A: Does not affect catalytic efficiency.
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
39% identity, 92% coverage: 23:274/274 of query aligns to 4:251/252 of 6vspB
5h5xC Crystal structure of nadh bound carbonyl reductase from streptomyces coelicolor
44% identity, 87% coverage: 26:264/274 of query aligns to 13:252/257 of 5h5xC
- active site: G24 (= G37), S151 (= S159), Y164 (= Y173), K168 (= K177)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G33), S23 (≠ G36), G24 (= G37), I25 (= I38), D44 (= D57), F45 (≠ V58), L69 (≠ T77), D70 (= D78), N97 (= N105), A98 (= A106), Y164 (= Y173), K168 (= K177), P194 (= P203), G195 (= G204), I197 (≠ V206), T199 (= T208)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
37% identity, 89% coverage: 23:266/274 of query aligns to 5:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G33), S17 (≠ G35), R18 (≠ G36), I20 (= I38), T40 (≠ V58), N62 (≠ D78), V63 (= V79), N89 (= N105), A90 (= A106), I92 (= I108), V139 (≠ T157), S141 (= S159), Y154 (= Y173), K158 (= K177), P184 (= P203), G185 (= G204), I187 (≠ V206), T189 (= T208), M191 (≠ L210)
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
40% identity, 89% coverage: 24:266/274 of query aligns to 3:251/256 of 7do7A
- active site: G16 (= G37), S146 (= S159), Y159 (= Y173)
- binding nicotinamide-adenine-dinucleotide: G12 (= G33), R15 (≠ G36), G16 (= G37), I17 (= I38), S37 (≠ V58), D66 (= D78), A67 (≠ V79), N93 (= N105), A94 (= A106), G95 (= G107), I96 (= I108), V144 (≠ T157), S145 (≠ A158), S146 (= S159), Y159 (= Y173), K163 (= K177), P189 (= P203), G190 (= G204), I192 (≠ V206), T194 (= T208), I196 (≠ L210)
- binding beta-L-rhamnopyranose: F99 (≠ P111), S146 (= S159), S148 (≠ V161), Q156 (= Q170), Y159 (= Y173), N197 (≠ L211), D235 (= D250), M236 (≠ D251), R238 (≠ S253)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
40% identity, 89% coverage: 24:266/274 of query aligns to 3:251/256 of 7b81A
- active site: G16 (= G37), S146 (= S159), Y159 (= Y173)
- binding nicotinamide-adenine-dinucleotide: G12 (= G33), S14 (≠ G35), R15 (≠ G36), I17 (= I38), D66 (= D78), A67 (≠ V79), N93 (= N105), A94 (= A106), G95 (= G107), I96 (= I108), T116 (≠ V130), V144 (≠ T157), S146 (= S159), Y159 (= Y173), K163 (= K177), P189 (= P203), G190 (= G204), I192 (≠ V206), T194 (= T208), I196 (≠ L210)
2hsdA The refined three-dimensional structure of 3alpha,20beta- hydroxysteroid dehydrogenase and possible roles of the residues conserved in short-chain dehydrogenases (see paper)
41% identity, 89% coverage: 24:267/274 of query aligns to 3:242/253 of 2hsdA
- active site: G16 (= G37), S138 (= S159), Y151 (= Y173), K155 (= K177)
- binding nicotinamide-adenine-dinucleotide: G12 (= G33), R15 (≠ G36), G16 (= G37), L17 (≠ I38), D36 (= D57), V37 (= V58), L58 (≠ T77), V60 (= V79), N86 (= N105), A87 (= A106), S138 (= S159), Y151 (= Y173), K155 (= K177), P181 (= P203), G182 (= G204), T184 (≠ V206)
1hdcA Mechanism of inhibition of 3alpha,20beta-hydroxysteroid dehydrogenase by a licorice-derived steroidal inhibitor (see paper)
41% identity, 89% coverage: 24:267/274 of query aligns to 3:242/253 of 1hdcA