SitesBLAST
Comparing WP_037571372.1 NCBI__GCF_000744815.1:WP_037571372.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
42% identity, 27% coverage: 426:611/687 of query aligns to 3:191/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G435), Q15 (≠ S438), G16 (= G439), I17 (= I440), D36 (= D459), V63 (= V485), N89 (= N511), A91 (≠ G513), S94 (≠ I516), I142 (= I562), S143 (≠ A563), S144 (= S564), Y157 (= Y577), K161 (= K581), P187 (= P607), H188 (≠ D608), I190 (≠ V610)
Sites not aligning to the query:
3rwbA Crystal structure of complex of 4pal (4-pyridoxolactone) and pldh (tetrameric pyridoxal 4-dehydrogenase) from mesorhizobium loti
37% identity, 37% coverage: 426:681/687 of query aligns to 4:244/247 of 3rwbA
- active site: G17 (= G439), S140 (= S564), Y153 (= Y577), K157 (= K581)
- binding 7-hydroxy-6-methylfuro[3,4-c]pyridin-1(3H)-one: S140 (= S564), N141 (= N566), T142 (≠ S567), M150 (≠ N574), Y153 (= Y577), L185 (≠ V610), H196 (≠ E633)
- binding nicotinamide-adenine-dinucleotide: G13 (= G435), Q16 (≠ S438), G17 (= G439), I18 (= I440), D37 (= D459), I38 (≠ L460), D60 (= D484), I61 (≠ V485), N87 (= N511), A88 (= A512), S89 (≠ G513), I138 (= I562), S140 (= S564), Y153 (= Y577), K157 (= K581), P183 (= P607), L185 (≠ V610), I186 (≠ V611), S188 (≠ G613), G190 (= G615), V191 (≠ I616)
3ndrA Crystal structure of tetrameric pyridoxal 4-dehydrogenase from mesorhizobium loti
37% identity, 37% coverage: 426:681/687 of query aligns to 4:244/247 of 3ndrA
- active site: G17 (= G439), S140 (= S564), Y153 (= Y577), K157 (= K581)
- binding nicotinamide-adenine-dinucleotide: G13 (= G435), Q16 (≠ S438), G17 (= G439), I18 (= I440), D37 (= D459), I38 (≠ L460), D60 (= D484), I61 (≠ V485), N87 (= N511), A88 (= A512), S89 (≠ G513), V110 (≠ I534), I138 (= I562), S140 (= S564), Y153 (= Y577), K157 (= K581), P183 (= P607), L185 (≠ V610), I186 (≠ V611), S188 (≠ G613), G190 (= G615), V191 (≠ I616)
F1SWA0 Zerumbone synthase; EC 1.1.1.326 from Zingiber zerumbet (Shampoo ginger) (Amomum zerumbet) (see paper)
34% identity, 38% coverage: 426:684/687 of query aligns to 3:259/267 of F1SWA0
- S142 (= S564) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- S144 (≠ N566) mutation to A: Increased oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- Y155 (= Y577) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- K159 (= K581) mutation to A: Abolishes all oxidoreductase activity.
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
32% identity, 38% coverage: 426:683/687 of query aligns to 5:254/255 of 5itvA
- active site: G18 (= G439), S141 (= S564), Y154 (= Y577), K158 (= K581)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G435), S17 (= S438), G18 (= G439), I19 (= I440), D38 (= D459), I39 (≠ L460), T61 (≠ V483), I63 (≠ V485), N89 (= N511), G91 (= G513), T139 (≠ I562), S141 (= S564), Y154 (= Y577), K158 (= K581), P184 (= P607), G185 (vs. gap), I186 (vs. gap), I187 (vs. gap)
3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
38% identity, 37% coverage: 429:681/687 of query aligns to 2:252/255 of 3wyeA
- active site: G12 (= G439), S138 (= S564), Y151 (= Y577), K155 (= K581), L196 (≠ T628)
- binding nicotinamide-adenine-dinucleotide: G8 (= G435), Q11 (≠ S438), G12 (= G439), I13 (= I440), D32 (= D459), Y33 (≠ L460), V57 (= V483), D58 (= D484), V59 (= V485), N85 (= N511), A86 (= A512), S138 (= S564), Y151 (= Y577), K155 (= K581), P181 (= P607), G182 (= G609), V184 (= V611), T186 (≠ S614), M188 (≠ I616), W189 (= W621)
6zzpA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and 3-oxovalerate (see paper)
35% identity, 35% coverage: 424:662/687 of query aligns to 6:243/265 of 6zzpA
- binding nicotinamide-adenine-dinucleotide: G17 (= G435), S20 (= S438), G21 (= G439), I22 (= I440), D41 (= D459), I42 (≠ L460), M66 (≠ V483), D67 (= D484), V68 (= V485), N94 (= N511), A95 (= A512), G96 (= G513), M145 (≠ I562), S147 (= S564), Y160 (= Y577), K164 (= K581), P190 (= P607), F192 (≠ V610), V193 (= V611), T195 (≠ G613), L197 (≠ F617), V198 (≠ A618)
- binding 3-oxidanylidenepentanoic acid: Q98 (≠ S515), S147 (= S564), H149 (≠ N566), K157 (≠ N574), Y160 (= Y577), F192 (≠ V610), Q201 (≠ W621)
6zzoC Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and acetoacetate (see paper)
35% identity, 35% coverage: 424:662/687 of query aligns to 6:243/265 of 6zzoC
- binding acetoacetic acid: Q98 (≠ S515), H149 (≠ N566), K157 (≠ N574), F192 (≠ V610), Q201 (≠ W621)
- binding nicotinamide-adenine-dinucleotide: G17 (= G435), S20 (= S438), G21 (= G439), I22 (= I440), D41 (= D459), I42 (≠ L460), M66 (≠ V483), D67 (= D484), V68 (= V485), N94 (= N511), A95 (= A512), G96 (= G513), M145 (≠ I562), Y160 (= Y577), K164 (= K581), P190 (= P607), F192 (≠ V610), V193 (= V611), T195 (≠ G613), L197 (≠ F617), V198 (≠ A618)
8dt1C Crystal structure of a putative d-beta-hydroxybutyrate dehydrogenase from burkholderia cenocepacia j2315 in complex with NAD
38% identity, 31% coverage: 426:636/687 of query aligns to 2:210/259 of 8dt1C
- binding nicotinamide-adenine-dinucleotide: G11 (= G435), S14 (= S438), G15 (= G439), I16 (= I440), D35 (= D459), L36 (= L460), M60 (≠ V483), V62 (= V485), N88 (= N511), A89 (= A512), M139 (≠ I562), Y154 (= Y577), K158 (= K581), P184 (= P607), G185 (= G609), F186 (≠ V610), V187 (= V611), T189 (≠ G613)
7ejiB Crystal structure of kred f147l/l153q/y190p/l199a/m205f/m206f variant and methyl methacrylate complex
32% identity, 38% coverage: 426:683/687 of query aligns to 4:250/251 of 7ejiB
- binding methyl 2-methylprop-2-enoate: S142 (= S564), I143 (≠ K565), Y155 (= Y577), F205 (≠ Y640)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G435), T15 (≠ A437), L16 (≠ S438), G17 (= G439), I18 (= I440), R38 (≠ L460), H39 (≠ N461), D62 (= D484), A63 (≠ V485), N89 (= N511), A90 (= A512), V112 (≠ I534), M140 (≠ I562), S142 (= S564), Y155 (= Y577), K159 (= K581), P187 (= P607), P189 (≠ V610), I190 (≠ V611), T192 (≠ S614), P193 (≠ G615), L194 (≠ I616)
7ejhA Crystal structure of kred mutant-f147l/l153q/y190p/l199a/m205f/m206f and 2-hydroxyisoindoline-1,3-dione complex
32% identity, 38% coverage: 426:683/687 of query aligns to 6:252/253 of 7ejhA
- binding 2-oxidanylisoindole-1,3-dione: S144 (= S564), I145 (≠ K565), E146 (≠ N566), Y157 (= Y577), V197 (≠ F617), F207 (≠ Y640)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G435), T17 (≠ A437), I20 (= I440), R40 (≠ L460), H41 (≠ N461), D64 (= D484), A65 (≠ V485), N91 (= N511), A92 (= A512), V114 (≠ I534), M142 (≠ I562), S144 (= S564), Y157 (= Y577), K161 (= K581), P189 (= P607), G190 (= G609), P191 (≠ V610), I192 (≠ V611), T194 (≠ S614), P195 (≠ G615), L196 (≠ I616)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
34% identity, 37% coverage: 429:681/687 of query aligns to 3:253/256 of Q48436
- 6:33 (vs. 432:459, 64% identical) binding NAD(+)
- D59 (= D484) binding NAD(+)
- K156 (= K581) binding NAD(+)
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
35% identity, 37% coverage: 429:681/687 of query aligns to 3:253/256 of 1gegE
- active site: G13 (= G439), S139 (= S564), Y152 (= Y577), K156 (= K581), V197 (vs. gap)
- binding alpha-D-glucopyranose: R63 (≠ E488), D64 (≠ A489), F67 (≠ A492), E123 (≠ R548)
- binding nicotinamide-adenine-dinucleotide: G9 (= G435), Q12 (≠ S438), I14 (= I440), D33 (= D459), Y34 (≠ L460), V58 (= V483), D59 (= D484), V60 (= V485), N86 (= N511), A87 (= A512), I109 (= I534), S139 (= S564), Y152 (= Y577), K156 (= K581), P182 (= P607), V185 (= V611), T187 (≠ S614), M189 (≠ I616)
Q920P0 L-xylulose reductase; XR; Dicarbonyl/L-xylulose reductase; EC 1.1.1.10 from Rattus norvegicus (Rat) (see paper)
36% identity, 38% coverage: 426:683/687 of query aligns to 5:243/244 of Q920P0
- S136 (= S564) mutation to A: Loss of activity.
- Q137 (≠ K565) mutation to M: Slightly reduced activity. Loss of activity for sugars; when associated with F-143 and L-146.
- L143 (≠ G571) mutation to F: Slightly reduced activity. Loss of activity for sugars; when associated with M-137 and L-146.
- H146 (≠ N574) mutation to L: Slightly reduced activity. Loss of activity for sugars; when associated with M-137; F-143 and L-146.
- Y149 (= Y577) mutation to F: Loss of activity.
- K153 (= K581) mutation to M: Loss of activity.
- N190 (= N619) mutation to V: Slightly reduced activity. Loss of activity for sugars; when associated with S-191.
- W191 (= W621) mutation to F: Slightly reduced activity.; mutation to S: Slightly reduced activity. Loss of activity for sugars; when associated with V-190.
2d1yA Crystal structure of tt0321 from thermus thermophilus hb8 (see paper)
36% identity, 37% coverage: 432:685/687 of query aligns to 9:240/240 of 2d1yA
- active site: G16 (= G439), S135 (= S564), N145 (= N574), Y148 (= Y577), K152 (= K581)
- binding nicotinamide-adenine-dinucleotide: G12 (= G435), R15 (≠ S438), I17 (= I440), D36 (= D459), L37 (= L460), R38 (≠ N461), V55 (= V483), D56 (= D484), L57 (≠ V485), N83 (= N511), A84 (= A512), A85 (≠ G513), I86 (= I514), V133 (≠ I562), S135 (= S564), Y148 (= Y577), K152 (= K581), P178 (= P607), G179 (≠ D608), I181 (≠ V610), T183 (≠ Q624), A185 (= A626), V186 (≠ A627)
P08074 Carbonyl reductase [NADPH] 2; Adipocyte protein P27; AP27; Lung carbonyl reductase; LCR; NADPH-dependent carbonyl reductase 2; EC 1.1.1.184 from Mus musculus (Mouse) (see 2 papers)
35% identity, 37% coverage: 431:684/687 of query aligns to 10:244/244 of P08074
- 11:39 (vs. 432:459, 55% identical) binding NADP(+)
- T38 (≠ A458) mutation to R: Converts the coenzyme specificity from NADP to NAD.
3d3wA Structure of l-xylulose reductase with bound coenzyme, phosphate and hydroxide. (see paper)
36% identity, 38% coverage: 426:684/687 of query aligns to 5:244/244 of 3d3wA