SitesBLAST
Comparing WP_037572389.1 NCBI__GCF_000744815.1:WP_037572389.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
46% identity, 84% coverage: 19:343/386 of query aligns to 18:343/378 of P69874
- C26 (≠ K27) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F28) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (= F46) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C55) mutation to T: Loss of ATPase activity and transport.
- L60 (= L61) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L77) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V134) mutation to M: Loss of ATPase activity and transport.
- D172 (= D171) mutation to N: Loss of ATPase activity and transport.
- C276 (≠ L274) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E295) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
46% identity, 84% coverage: 19:343/386 of query aligns to 3:328/358 of 8y5iA
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
42% identity, 93% coverage: 18:375/386 of query aligns to 6:352/353 of 1vciA
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
48% identity, 62% coverage: 17:257/386 of query aligns to 5:255/375 of 2d62A
1g291 Malk (see paper)
50% identity, 60% coverage: 19:248/386 of query aligns to 4:241/372 of 1g291
- binding magnesium ion: D69 (= D84), E71 (≠ K86), K72 (vs. gap), K79 (≠ Y88), D80 (≠ R89)
- binding pyrophosphate 2-: S38 (= S53), G39 (= G54), C40 (= C55), G41 (= G56), K42 (= K57), T43 (= T58), T44 (= T59)
Sites not aligning to the query:
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
40% identity, 85% coverage: 17:345/386 of query aligns to 1:331/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F28), S37 (= S53), G38 (= G54), C39 (= C55), G40 (= G56), K41 (= K57), S42 (≠ T58), T43 (= T59), Q81 (= Q97), R128 (= R142), A132 (≠ Q146), S134 (= S148), G136 (= G150), Q137 (= Q151), E158 (= E172), H191 (= H205)
- binding magnesium ion: S42 (≠ T58), Q81 (= Q97)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
40% identity, 85% coverage: 17:345/386 of query aligns to 1:331/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F28), G38 (= G54), C39 (= C55), G40 (= G56), K41 (= K57), S42 (≠ T58), T43 (= T59), R128 (= R142), S134 (= S148), Q137 (= Q151)
- binding beryllium trifluoride ion: S37 (= S53), G38 (= G54), K41 (= K57), Q81 (= Q97), S134 (= S148), G136 (= G150), H191 (= H205)
- binding magnesium ion: S42 (≠ T58), Q81 (= Q97)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
40% identity, 85% coverage: 17:345/386 of query aligns to 1:331/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F28), V17 (≠ A33), G38 (= G54), C39 (= C55), G40 (= G56), K41 (= K57), S42 (≠ T58), T43 (= T59), R128 (= R142), A132 (≠ Q146), S134 (= S148), Q137 (= Q151)
- binding tetrafluoroaluminate ion: S37 (= S53), G38 (= G54), K41 (= K57), Q81 (= Q97), S134 (= S148), G135 (= G149), G136 (= G150), E158 (= E172), H191 (= H205)
- binding magnesium ion: S42 (≠ T58), Q81 (= Q97)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
40% identity, 85% coverage: 17:345/386 of query aligns to 1:331/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F28), V17 (≠ A33), G38 (= G54), C39 (= C55), G40 (= G56), K41 (= K57), S42 (≠ T58), T43 (= T59), R128 (= R142), A132 (≠ Q146), S134 (= S148), Q137 (= Q151)
- binding magnesium ion: S42 (≠ T58), Q81 (= Q97)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
40% identity, 85% coverage: 17:345/386 of query aligns to 2:332/371 of P68187
- A85 (= A100) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ R121) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V127) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ M130) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ D132) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ G137) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G150) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D171) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ T241) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ L252) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ A275) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (≠ S285) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ I289) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (= G291) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G315) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ S321) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
- S322 (= S335) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
40% identity, 85% coverage: 17:345/386 of query aligns to 1:331/374 of 2awnB
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
40% identity, 85% coverage: 19:345/386 of query aligns to 1:329/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F28), S35 (= S53), G36 (= G54), C37 (= C55), G38 (= G56), K39 (= K57), S40 (≠ T58), T41 (= T59), R126 (= R142), A130 (≠ Q146), S132 (= S148), G134 (= G150), Q135 (= Q151)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
39% identity, 85% coverage: 17:345/386 of query aligns to 2:330/369 of P19566
- L86 (= L101) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P173) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D178) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ S321) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
3d31A Modbc from methanosarcina acetivorans (see paper)
36% identity, 90% coverage: 19:364/386 of query aligns to 2:342/348 of 3d31A