SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_037572586.1 NCBI__GCF_000744815.1:WP_037572586.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
44% identity, 95% coverage: 26:613/618 of query aligns to 89:664/664 of P09114

query
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P09114

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P191 (≠ A128) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W508) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
44% identity, 95% coverage: 26:613/618 of query aligns to 92:667/667 of P09342

query
sites
P09342

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C161 (= C95) modified: Disulfide link with 307
P194 (≠ A128) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V239) modified: Disulfide link with 161

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 12:581/582 of 3ea4A

query
sites
3ea4A

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active site: Y32 (≠ L49), G34 (= G51), G35 (= G52), A36 (= A53), S37 (≠ I54), E58 (= E75), T81 (= T98), F120 (= F137), Q121 (= Q138), E122 (= E139), K170 (= K187), M265 (= M282), V292 (= V309), V399 (= V419), G425 (= G445), M427 (= M447), D452 (= D472), N479 (= N499), H481 (≠ S501), L482 (= L502), M484 (= M504), V485 (= V505), W488 (= W508), H557 (≠ Q586)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D307), R291 (= R308), W488 (= W508), S567 (≠ A596)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R177), G221 (= G236), G222 (= G237), G223 (= G238), T245 (= T262), L246 (= L263), M247 (= M264), L263 (≠ P280), G264 (= G281), M265 (= M282), H266 (= H283), G285 (= G302), R287 (= R304), D289 (= D306), R291 (= R308), D309 (= D326), I310 (= I327), G327 (= G344), D328 (= D345), V329 (≠ A346), M404 (= M424), G422 (= G442)
binding magnesium ion: D452 (= D472), N479 (= N499), H481 (≠ S501)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V419), G400 (= G420), Q401 (= Q421), H402 (= H422), M427 (= M447), G451 (= G471), D452 (= D472), G453 (= G473), S454 (≠ C474), N479 (= N499), H481 (≠ S501), L482 (= L502), G483 (= G503), M484 (= M504), V485 (= V505)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 12:581/582 of 3e9yA

query
sites
3e9yA

S

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active site: Y32 (≠ L49), G34 (= G51), G35 (= G52), A36 (= A53), S37 (≠ I54), E58 (= E75), T81 (= T98), F120 (= F137), Q121 (= Q138), E122 (= E139), K170 (= K187), M265 (= M282), V292 (= V309), V399 (= V419), G425 (= G445), M427 (= M447), D452 (= D472), N479 (= N499), H481 (≠ S501), L482 (= L502), M484 (= M504), V485 (= V505), W488 (= W508), H557 (≠ Q586)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D307), R291 (= R308), W488 (= W508), S567 (≠ A596)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R177), G221 (= G236), G222 (= G237), G223 (= G238), T245 (= T262), L246 (= L263), M247 (= M264), L263 (≠ P280), G285 (= G302), R287 (= R304), D289 (= D306), R291 (= R308), D309 (= D326), I310 (= I327), G327 (= G344), D328 (= D345), V329 (≠ A346), M404 (= M424), G422 (= G442)
binding magnesium ion: D452 (= D472), N479 (= N499), H481 (≠ S501)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V419), G400 (= G420), Q401 (= Q421), H402 (= H422), M427 (= M447), G451 (= G471), G453 (= G473), S454 (≠ C474), N479 (= N499), H481 (≠ S501), L482 (= L502), G483 (= G503), M484 (= M504), V485 (= V505)

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 7tzzA

query
sites
7tzzA

S

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A

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T

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S

G

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I

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W

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Q

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E

L

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N

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K

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A

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x
H

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L

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M

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V

R

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Q

W
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W

Q

E

T

D

L

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F

Y

Y

N

K

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N

Y

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N

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L

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L

L

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A

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A

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C

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G

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A

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K

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L

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H

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V

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L

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P

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M

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I

A

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A
x
S

G

G

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G

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T

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F

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I

A

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D

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M282), R292 (= R308), W489 (= W508), S568 (≠ A596)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), G452 (= G471), D453 (= D472), G454 (= G473), S455 (≠ C474), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), M263 (= M279), L264 (≠ P280), M266 (= M282), H267 (= H283), G286 (= G302), R288 (= R304), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442)
binding magnesium ion: A37 (= A53), T82 (= T98), S83 (= S99), Q122 (= Q138), Y381 (≠ Q400), D453 (= D472), M458 (= M477), Q461 (= Q480), N480 (= N499), H482 (≠ S501), K533 (≠ P560)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/583 of 5k3sA

query
sites
5k3sA

S

K

G

G

A

A

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M

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I

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N

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E

N

N

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K

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L

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x
H

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L

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M
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M

V
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V

R

M

Q

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W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

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F

L

L

H

G

S

D

G

-

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-

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-

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-

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-

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-

K

-

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-

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P

A

A

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G

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E

I

I

-

F

P

P

D

N

F

M

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L

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F

A

A

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A

A

A

M

C

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C

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L

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A

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A

I

I

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T

A

M

R

L

S

D

L

-

N

T

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A

G

P

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V

Y

V

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I

L

D

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F

V

I

I

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C

H

P

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x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

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A

S

G
|
G

T

G

N

T

N

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D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R308), M485 (= M504), W489 (= W508), G569 (= G597)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), M266 (= M282), G286 (= G302), R288 (= R304), D290 (= D306), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), D453 (= D472), G454 (= G473), S455 (≠ C474), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
44% identity, 95% coverage: 29:613/618 of query aligns to 98:670/670 of P17597

query
sites
P17597

S

K

G

G

A

A

Q

D

S

I

L

L

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E

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A

L

L

E

E

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G

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F

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R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S
|
S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A
x
P

S

R

T
x
R

S

M

I

I

G

G

T

T

D

D

A

A

F

F

Q
|
Q

E

E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K
|
K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K
|
K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

H

M

Q

R

L

L

P

P

G

G

Y

Y

-

M

-

S

R

R

P

M

V

P

T

K

R

P

P

P

H

E

G

D

K

S

Q

H

I

L

R

E

E

Q

A

I

A

V

R

R

L

L

L

I

A

S

Q

E

A

S

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

G
|
G

G

G

V

C

L

L

K

N

A

-

R

-

A

S

T

S

A

D

E

E

L

L

K

G

I

R

L

F

A

V

E

E

L

L

T

T

K

G

A

I

P

P

V

V

T

A

T

S

T
|
T

L
|
L

M

M

G

G

I

L

G

G

A

S

F

Y

P

P

D
x
C

S

D

H

D

P

E

Q

L

H

S

L

L

G

H

M

M

P
x
L

G
|
G

M
|
M

H
|
H

G

G

T

T

V

V

A

Y

A

A

V

N

T

Y

S

A

L

V

Q

E

K

H

A

S

D

D

L

L

I

L

A

A

L

F

G
|
G

A
x
V

R
|
R

F
|
F

D
|
D

R
|
R

V

V

T

T

G

G

K

K

L

L

D

E

G

A

F

F

A

A

P

S

Y

R

A

A

T

K

I

I

V

V

H

H

A

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

N

N

R

K

A

T

A

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

E

L

V

A

L

L

A

Q

D

G

L

M

I

N

V

K

A

V

V

L

Q

E

N

N

E

R

L

A

D

E

H

E

T

L

P

-

G

-

A

K

C

L

D

D

Y

F

T

G

A

V

W

W

W

R

E

N

Q

E

L

L

G

N

G

V

W

Q

R

K

K

Q

T

K

Y

F

P

P

L

L

G

S

W

F

D

K

P

T

A

F

P

G

D

E

G

A

L

-

L

I

T

P

P

P

Q

Q

Q

Y

V

A

I

I

E

K

R

V

I

L

G

D

Q

E

L

L

V

T

G

D

P

G

D

K

A

A

I

I

Y

I

A

S

A

T

G

G

V

V

G

G

Q
|
Q

H
|
H

Q

Q

M

M

W

W

A

A

S

A

Q

Q

F

F

I

Y

Q

N

F

Y

E

K

H

K

P

P

A

R

T

Q

W

W

L

L

N

S

S

S

G
|
G

A

L

G
|
G

T
x
A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

M

I

G

G

A

A

K

S

A

V

G

A

Q

N

P

P

G

D

T

A

E

I

V

V

W

V

A

D

I

I

D

D

G

G

D
|
D

G
|
G

C
x
S

F

F

Q

I

M

M

T

N

N

V

Q

Q

E

E

L

L

V

A

T

T

C

I

A

R

L

V

N

E

N

N

I

L

P

P

I

V

K

K

V

V

A

L

V

L

I

L

N

N

N
|
N

G
x
Q

S
x
H

L
|
L

G
|
G

M
|
M

V

V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

Q

Q

T

A

M

R

L

S

D

L

-

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q

H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

P

I

D

K

F

Y

A122 (= A53) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L55) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E75) binding thiamine diphosphate
S186 (= S117) binding FAD
P197 (≠ A128) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ T130) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q138) binding thiamine diphosphate
K220 (= K151) binding (R)-imazaquin
R246 (= R177) binding (R)-imazaquin; binding FAD
K256 (= K187) binding chlorimuron-ethyl
G308 (= G237) binding FAD
TL 331:332 (= TL 262:263) binding FAD
C340 (≠ D271) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ PGMH 280:283) binding FAD
GVRFD 371:375 (≠ GARFD 302:306) binding FAD
DR 376:377 (= DR 307:308) binding chlorimuron-ethyl
DI 395:396 (= DI 326:327) binding FAD
DV 414:415 (≠ DA 345:346) binding FAD
QH 487:488 (= QH 421:422) binding thiamine diphosphate
GG 508:509 (= GG 442:443) binding FAD
GAM 511:513 (≠ GTM 445:447) binding thiamine diphosphate
D538 (= D472) binding Mg(2+)
DGS 538:540 (≠ DGC 472:474) binding thiamine diphosphate
N565 (= N499) binding Mg(2+)
NQHLGM 565:570 (≠ NGSLGM 499:504) binding thiamine diphosphate
H567 (≠ S501) binding Mg(2+)
W574 (= W508) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A596) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/585 of 5k2oA

query
sites
5k2oA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K
|
K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

H

M

Q

R

L

L

P

P

G

G

Y

Y

-

M

-

S

R

R

P

M

V

P

T

K

R

P

P

P

H

E

G

D

K

S

Q

H

I

L

R

E

E

Q

A

I

A

V

R

R

L

L

L

I

A

S

Q

E

A

S

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

V

C

L

L

K

N

A

-

R

-

A

S

T

S

A

D

E

E

L

L

K

G

I

R

L

F

A

V

E

E

L

L

T

T

K

G

A

I

P

P

V

V

T

A

T

S

T
|
T

L
|
L

M
|
M

G

G

I

L

G

G

A

S

F

Y

P

P

D

C

S

D

H

D

P

E

Q

L

H

S

L

L

G

H

M

M

P
x
L

G

G

M
|
M

H

H

G

G

T

T

V

V

A

Y

A

A

V

N

T

Y

S

A

L

V

Q

E

K

H

A

S

D

D

L

L

I

L

A

A

L

F

G
|
G

A

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

K

K

L

L

D

E

G

A

F

F

A

A

P

S

Y

R

A

A

T

K

I

I

V

V

H

H

A

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

N

N

R

K

A

T

A

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

E

L

V

A

L

L

A

Q

D

G

L

M

I

N

V

K

A

V

V

L

Q

E

N

N

E

R

L

A

D

E

H

E

T

L

P

-

G

-

A

K

C

L

D

D

Y

F

T

G

A

V

W

W

W

R

E

N

Q

E

L

L

G

N

G

V

W

Q

R

K

K

Q

T

K

Y

F

P

P

L

L

G

S

W

F

D

K

P

T

A

F

P

G

D

E

G

A

L

-

L

I

T

P

P

P

Q

Q

Q

Y

V

A

I

I

E

K

R

V

I

L

G

D

Q

E

L

L

V

T

G

D

P

G

D

K

A

A

I

I

Y

I

A

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q
|
Q

M
|
M

W

W

A

A

S

A

Q

Q

F

F

I

Y

Q

N

F

Y

E

K

H

K

P

P

A

R

T

Q

W

W

L

L

N

S

S

S

G
|
G

G

G

A

L

G
|
G

T

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

M

I

G

G

A

A

K

S

A

V

G

A

Q

N

P

P

G

D

T

A

E

I

V

V

W

V

A

D

I

I

D

D

G

G

D
|
D

G
|
G

C
x
S

F

F

Q

I

M

M

T

N

N

V

Q

Q

E

E

L

L

V

A

T

T

C

I

A

R

L

V

N

E

N

N

I

L

P

P

I

V

K

K

V

V

A

L

V

L

I

L

N

N

N
|
N

G

Q

S
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q
x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M282), R292 (= R308), W489 (= W508), S568 (≠ A596)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), G286 (= G302), R288 (= R304), D290 (= D306), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), Q404 (= Q423), M405 (= M424), G423 (= G442)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), M428 (= M447), D453 (= D472), G454 (= G473), S455 (≠ C474), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)

9c4rA Acetolactate synthase, chloroplastic (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 9c4rA

query
sites
9c4rA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L

Y

P

P

G

G

A

A

I

S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E

E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K

K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

H

M

Q

R

L

L

P

P

G

G

Y

Y

-

M

-

S

R

R

P

M

V

P

T

K

R

P

P

P

H

E

G

D

K

S

Q

H

I

L

R

E

E

Q

A

I

A

V

R

R

L

L

L

I

A

S

Q

E

A

S

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

V

C

L

L

K

N

A

-

R

-

A

S

T

S

A

D

E

E

L

L

K

G

I

R

L

F

A

V

E

E

L

L

T

T

K

G

A

I

P

P

V

V

T

A

T

S

T
|
T

L
|
L

M
|
M

G

G

I

L

G

G

A

S

F

Y

P

P

D

C

S

D

H

D

P

E

Q

L

H

S

L

L

G

H

M

M

P
x
L

G

G

M
|
M

H
|
H

G

G

T

T

V

V

A

Y

A

A

V

N

T

Y

S

A

L

V

Q

E

K

H

A

S

D

D

L

L

I

L

A

A

L

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

K

K

L

L

D

E

G

A

F

F

A

A

P

S

Y

R

A

A

T

K

I

I

V

V

H

H

A

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

N

N

R

K

A

T

A

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

E

L

V

A

L

L

A

Q

D

G

L

M

I

N

V

K

A

V

V

L

Q

E

N

N

E

R

L

A

D

E

H

E

T

L

P

-

G

-

A

K

C

L

D

D

Y

F

T

G

A

V

W

W

W

R

E

N

Q

E

L

L

G

N

G

V

W

Q

R

K

K

Q

T

K

Y

F

P

P

L

L

G

S

W

F

D

K

P

T

A

F

P

G

D

E

G

A

L

-

L

I

T

P

P

P

Q

Q

Q

Y

V

A

I

I

E

K

R

V

I

L

G

D

Q

E

L

L

V

T

G

D

P

G

D

K

A

A

I

I

Y

I

A

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

S

A

Q

Q

F

F

I

Y

Q

N

F

Y

E

K

H

K

P

P

A

R

T

Q

W

W

L

L

N

S

S

S

G
|
G

G

G

A

L

G
|
G

T

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

M

I

G

G

A

A

K

S

A

V

G

A

Q

N

P

P

G

D

T

A

E

I

V

V

W

V

A

D

I

I

D

D

G
|
G

D
|
D

G
|
G

C
x
S

F

F

Q

I

M
|
M

T

N

N

V

Q

Q

E

E

L

L

V

A

T

T

C

I

A

R

L

V

N

E

N

N

I

L

P

P

I

V

K

K

V

V

A

L

V

L

I

L

N

N

N
|
N

G

Q

S
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q

H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

binding 2-(2-chloroethoxy)-N-[(4-methoxy-6-methylpyrimidin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (= M282), R292 (= R308), W489 (= W508), S568 (≠ A596)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), G452 (= G471), G454 (= G473), S455 (≠ C474), M458 (= M477), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)

9c4qA Acetolactate synthase, chloroplastic (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 9c4qA

query
sites
9c4qA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L

Y

P

P

G

G

A

A

I

S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E

E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K

K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

H

M

Q

R

L

L

P

P

G

G

Y

Y

-

M

-

S

R

R

P

M

V

P

T

K

R

P

P

P

H

E

G

D

K

S

Q

H

I

L

R

E

E

Q

A

I

A

V

R

R

L

L

L

I

A

S

Q

E

A

S

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

V

C

L

L

K

N

A

-

R

-

A

S

T

S

A

D

E

E

L

L

K

G

I

R

L

F

A

V

E

E

L

L

T

T

K

G

A

I

P

P

V

V

T

A

T

S

T
|
T

L
|
L

M
|
M

G

G

I

L

G

G

A

S

F

Y

P

P

D

C

S

D

H

D

P

E

Q

L

H

S

L

L

G

H

M
|
M

P
x
L

G

G

M
|
M

H
|
H

G

G

T

T

V

V

A

Y

A

A

V

N

T

Y

S

A

L

V

Q

E

K

H

A

S

D

D

L

L

I

L

A

A

L

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

L

L

D

E

G

A

F

F

A

A

P

S

Y

R

A

A

T

K

I

I

V

V

H

H

A

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

N

N

R

K

A

T

A

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

E

L

V

A

L

L

A

Q

D

G

L

M

I

N

V

K

A

V

V

L

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N

N

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R

L

A

D

E

H

E

T

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P

-

G

-

A

K

C

L

D

D

Y

F

T

G

A

V

W

W

W

R

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N

Q

E

L

L

G

N

G

V

W

Q

R

K

K

Q

T

K

Y

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P

P

L

L

G

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W

F

D

K

P

T

A

F

P

G

D

E

G

A

L

-

L

I

T

P

P

P

Q

Q

Q
x
Y

V

A

I

I

E

K

R

V

I

L

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D

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E

L

L

V

T

G

D

P

G

D

K

A

A

I

I

Y

I

A

S

A

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

S

A

Q

Q

F

F

I

Y

Q

N

F

Y

E

K

H

K

P

P

A

R

T

Q

W

W

L

L

N

S

S

S

G
|
G

G

G

A

L

G
|
G

T

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

M

I

G

G

A

A

K

S

A

V

G

A

Q

N

P

P

G

D

T

A

E

I

V

V

W

V

A

D

I

I

D

D

G
|
G

D
|
D

G
|
G

C
x
S

F

F

Q

I

M
|
M

T

N

N

V

Q
|
Q

E

E

L

L

V

A

T

T

C

I

A

R

L

V

N

E

N

N

I

L

P

P

I

V

K

K

V

V

A

L

V

L

I

L

N

N

N
|
N

G

Q

S
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P
x
K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q

H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

binding 2-(2-fluoroethoxy)-N-[(4-methoxy-6-methylpyrimidin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (= M282), D291 (= D307), R292 (= R308), W489 (= W508), S568 (≠ A596)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), G452 (= G471), D453 (= D472), G454 (= G473), S455 (≠ C474), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), M263 (= M279), L264 (≠ P280), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), R292 (= R308), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442)
binding magnesium ion: Y381 (≠ Q400), D453 (= D472), N480 (= N499), H482 (≠ S501), K533 (≠ P560)
binding oxygen molecule: M458 (= M477), Q461 (= Q480)

9c4pA Acetolactate synthase, chloroplastic (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 9c4pA

query
sites
9c4pA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L

Y

P

P

G

G

A

A

I

S

L

M

P

E

A

I

Y

H

D

Q

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A

L

L

M

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S

S

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S

K

S

L

I

R

R

H

N

V

V

L

L

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R

R

H

H

E

E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F

F

Q

Q

E

E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K

K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

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N

W

W

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M

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L

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P

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M

-

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P

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K

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L

L

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A

S

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A

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K

P

P

V

V

L

L

Y

Y

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G
|
G

V

C

L

L

K

N

A

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R

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A

S

T

S

A

D

E

E

L

L

K

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I

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L

F

A

V

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E

L

L

T

T

K

G

A

I

P

P

V

V

T

A

T

S

T
|
T

L
|
L

M
|
M

G

G

I

L

G

G

A

S

F

Y

P

P

D

C

S

D

H

D

P

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Q

L

H

S

L

L

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H

M
|
M

P
x
L

G

G

M
|
M

H
|
H

G

G

T

T

V

V

A

Y

A

A

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N

T

Y

S

A

L

V

Q

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K

H

A

S

D

D

L

L

I

L

A

A

L

F

G
|
G

A
x
V

R
|
R

F

F

D
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D

R
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R

V
|
V

T

T

G

G

K

K

L

L

D

E

G

A

F

F

A

A

P

S

Y

R

A

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K

I

I

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V

H

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I

D
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D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

N

N

R

K

A

T

A

P

D

H

V

V

P

S

I

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V

C

G

G

D
|
D

A
x
V

R

K

E

L

V

A

L

L

A

Q

D

G

L

M

I

N

V

K

A

V

V

L

Q

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N

N

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R

L

A

D

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H

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T

L

P

-

G

-

A

K

C

L

D

D

Y

F

T

G

A

V

W

W

W

R

E

N

Q

E

L

L

G

N

G

V

W

Q

R

K

K

Q

T

K

Y

F

P

P

L

L

G

S

W

F

D

K

P

T

A

F

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G

D

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G

A

L

-

L

I

T

P

P

P

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Q

Q

Y

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I

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K

R

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D

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L

L

V

T

G

D

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G

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K

A

A

I

I

Y

I

A

S

A

T

G

G

V
|
V

G
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G

Q
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Q

H
|
H

Q

Q

M
|
M

W

W

A

A

S

A

Q

Q

F

F

I

Y

Q

N

F

Y

E

K

H

K

P

P

A

R

T

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W

W

L

L

N

S

S

S

G
|
G

A

L

G
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G

T

A

M
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M

G

G

Y

F

A

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V

L

P

P

A

A

M

I

G

G

A

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K

S

A

V

G

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N

P

P

G

D

T

A

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I

V

V

W

V

A

D

I

I

D

D

G
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G

D
|
D

G
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G

C
x
S

F

F

Q

I

M
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M

T

N

N

V

Q

Q

E

E

L

L

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A

T

T

C

I

A

R

L

V

N

E

N

N

I

L

P

P

I

V

K

K

V

V

A

L

V

L

I

L

N

N

N
|
N

G

Q

S
x
H

L
|
L

G
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G

M
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M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

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Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q

H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

binding 2-(2-chloroethoxy)-N-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)carbamoyl]benzene-1-sulfonamide: M266 (= M282), D291 (= D307), R292 (= R308), M485 (= M504), W489 (= W508), S568 (≠ A596)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), G452 (= G471), D453 (= D472), G454 (= G473), S455 (≠ C474), M458 (= M477), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), M263 (= M279), L264 (≠ P280), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), R292 (= R308), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442), G424 (= G443)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 8et4A

query
sites
8et4A

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L

Y

P

P

G

G

A

A

I

S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E

E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F

F

Q

Q

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E

A

T

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P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

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G

G

R
|
R

P

P

G

G

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V

L

L

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D

D

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K

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D

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L

L

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A

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P

V

V

L

L

Y

Y

V

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G
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G

V

C

L

L

K

N

A

-

R

-

A

S

T

S

A

D

E

E

L

L

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G

I

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L

F

A

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L

L

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T

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L

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M

G

G

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G

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C

S

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H

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L

L

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M

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x
L

G

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M

H
|
H

G

G

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T

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Y

A

A

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N

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L

L

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A

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G

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x
V

R
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R

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F

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|
D

D

D

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R

V
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V

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T

G

G

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K

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L

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G

A

F

F

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Y

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H

H

A

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D
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D

I
|
I

D

D

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S

A

A

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I

G

G

K

K

N

N

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K

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D

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F

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L

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G

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A

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A

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G

G

V
|
V

G
|
G

Q
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Q

H
|
H

Q

Q

M
|
M

W

W

A

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A

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|
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N

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K

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L

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N

N
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N

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x
H

L
|
L

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|
G

M
|
M

V
|
V

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|
W

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x
K

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M

H

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W

L

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M

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x
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G

G

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N

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I

V

M

I

A

T

A

E

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D

D

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G

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R

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), G452 (= G471), D453 (= D472), G454 (= G473), S455 (≠ C474), M458 (= M477), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), R292 (= R308), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442)
binding magnesium ion: F370 (≠ W388), D453 (= D472), M458 (= M477), Q461 (= Q480), N480 (= N499), H482 (≠ S501), K533 (≠ P560)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M282), R292 (= R308), M485 (= M504), W489 (= W508), S568 (≠ A596)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 5wj1A

query
sites
5wj1A

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x
H

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M

H

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L

P

P

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M

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A
x
S

G

G

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G

N

T

N

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R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), M263 (= M279), L264 (≠ P280), G286 (= G302), R288 (= R304), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442), G424 (= G443)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M282), D291 (= D307), R292 (= R308), M485 (= M504), W489 (= W508), S568 (≠ A596)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), M428 (= M447), D453 (= D472), G454 (= G473), S455 (≠ C474), M458 (= M477), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 5k6tA

query
sites
5k6tA

S

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E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q
x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H283), R292 (= R308), M485 (= M504), W489 (= W508), S568 (≠ A596)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), G286 (= G302), R288 (= R304), D290 (= D306), R292 (= R308), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), Q404 (= Q423), M405 (= M424), G423 (= G442)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), G452 (= G471), G454 (= G473), S455 (≠ C474), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 5k6rA

query
sites
5k6rA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
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Q

E
|
E

A

T

D

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I

I

C

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G

E

I

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T

T

M

R

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S

I

I

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K

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H

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N

N

F

Y

L

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T

M

D

D

A

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A

E

D

D

I

I

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I

I

I

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E

E

E

A

A

F

F

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F

I

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A

A

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G

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R
|
R

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G

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L

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D

D

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A

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|
K

D

D

A

I

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T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

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Q

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L

L

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P

G

G

Y

Y

-

M

-

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D

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L

L

L

I

A

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A

S

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P

P

V

V

L

L

Y

Y

V

V

G
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G

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C

L

L

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N

A

-

R

-

A

S

T

S

A

D

E

E

L

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I

R

L

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A

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E

L

L

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T

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G

A

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T

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M

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x
L

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M

H

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Y

A

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D

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A

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G

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V

T

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G

G

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A

I

D
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D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

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N

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I

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C

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D

A
x
V

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A

L

L

A

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I

N

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A

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E

N

N

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A

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-

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-

A

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C

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D

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W

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R

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L

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W

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R

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K

Q

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Y

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L

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W

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D

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A

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D

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G

A

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-

L

I

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Q

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Y

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A

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L

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G

D

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A

A

I

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Y

I

A

S

A

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G

G

V
|
V

G
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G

Q
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Q

H
|
H

Q

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M
|
M

W

W

A

A

S

A

Q

Q

F

F

I

Y

Q

N

F

Y

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K

H

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P

P

A

R

T

Q

W

W

L

L

N

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G

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A

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G

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M

G

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Y

F

A

G

V

L

P

P

A

A

M

I

G

G

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I

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V

W

V

A

D

I

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D

D

G

G

D
|
D

G
|
G

C
x
S

F

F

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I

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|
M

T

N

N

V

Q

Q

E

E

L

L

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A

T

T

C

I

A

R

L

V

N

E

N

N

I

L

P

P

I

V

K

K

V

V

A

L

V

L

I

L

N

N

N
|
N

G

Q

S
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q
x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R308), W489 (= W508), S568 (≠ A596)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), M266 (= M282), G286 (= G302), R288 (= R304), R292 (= R308), V293 (= V309), D310 (= D326), I311 (= I327), G328 (= G344), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), D453 (= D472), G454 (= G473), S455 (≠ C474), M458 (= M477), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 1z8nA

query
sites
1z8nA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

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S

R

T

R

S

M

I

I

G

G

T

T

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D

A

A

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F

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Q

E
|
E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K
|
K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

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P

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R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K
|
K

D

D

A

I

M

Q

Q

Q

K

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L

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W

W

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A

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M

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L

L

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G

G

Y
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Y

-

M

-

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R

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V

L

L

Y

Y

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V

G
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G

G

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G
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G

V

C

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A

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R

-

A

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H

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I
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G

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N

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L

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N

N

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K

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x
H

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R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q
x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A

S

G
|
G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K151), R161 (= R177), Y191 (= Y207), R194 (= R208), D291 (= D307), R292 (= R308), D312 (= D328), W489 (= W508), G569 (= G597)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), G265 (= G281), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), R292 (= R308), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442), G424 (= G443)
binding magnesium ion: D453 (= D472), N480 (= N499)
binding thiamine diphosphate: V400 (= V419), G401 (= G420), Q402 (= Q421), H403 (= H422), G426 (= G445), M428 (= M447), G452 (= G471), G454 (= G473), S455 (≠ C474), N480 (= N499), H482 (≠ S501), L483 (= L502), G484 (= G503), M485 (= M504), V486 (= V505)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 1yi1A

query
sites
1yi1A

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

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V

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E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

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T

R

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M

I

I

G

G

T

T

D

D

A

A

F
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F

Q
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Q

E
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E

A

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I

I

C

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G

E

I

V

T

T

M

R

P

S

I

I

T

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K

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H

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Y

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L

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V

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M

D

D

A

V

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E

D

D

I

I

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P

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V

I

I

I

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E

E

E

A

A

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H

F

I

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A

A

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T

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G

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R
|
R

P

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L

V

V

D

D

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A

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|
K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

H

M

Q

R

L

L

P

P

G

G

Y

Y

-

M

-

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R

P

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T

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I

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L

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I

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E

A

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P

V

V

L

L

Y

Y

V

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G

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G
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G

V

C

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N

A

-

R

-

A

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T

S

A

D

E

E

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I

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A

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E

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M

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x
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H
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H

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A

Y

A

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x
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R

V
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V

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T

G

G

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I

D
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D

I
|
I

D

D

P

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A

A

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E

I

I

G

G

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N

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R

K

A

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D

A
x
V

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L

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A

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I

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-

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-

A

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C

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G

A

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-

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A

A

I

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Y

I

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V

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Q

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M
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M

W

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A

A

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A

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F

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Y

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N

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Y

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A

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L

P

P

A

A

M

I

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A

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D

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N

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Q

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E

L

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A

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C

I

A

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L

V

N

E

N

N

I

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P

P

I

V

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K

V

V

A

L

V

L

I

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N

N
|
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x
H

L
|
L

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G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q
x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D307), R292 (= R308), W489 (= W508), S568 (≠ A596)
binding flavin-adenine dinucleotide: R161 (= R177), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), M263 (= M279), L264 (≠ P280), G265 (= G281), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442), G424 (= G443)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 1yi0A

query
sites
1yi0A

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

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P

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R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

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V

L

L

V

V

D

D

V

V

A

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K

D

D

A

I

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Q

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N

W

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G

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I

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L

I

L

N

N

N
|
N

G

Q

S
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q
x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D307), R292 (= R308), W489 (= W508), S568 (≠ A596)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), G265 (= G281), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), R292 (= R308), V293 (= V309), D310 (= D326), I311 (= I327), G328 (= G344), D329 (= D345), V330 (≠ A346), M405 (= M424), G423 (= G442), G424 (= G443)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 1yhzA

query
sites
1yhzA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K
|
K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

H

M

Q

R

L

L

P

P

G

G

Y

Y

-

M

-

S

R

R

P

M

V

P

T

K

R

P

P

P

H

E

G

D

K

S

Q

H

I

L

R

E

E

Q

A

I

A

V

R

R

L

L

L

I

A

S

Q

E

A

S

R

K

P

P

V

V

L

L

Y

Y

V

V

G

G

G
|
G

V

C

L

L

K

N

A

-

R

-

A

S

T

S

A

D

E

E

L

L

K

G

I

R

L

F

A

V

E

E

L

L

T

T

K

G

A

I

P

P

V

V

T

A

T

S

T
|
T

L
|
L

M
|
M

G

G

I

L

G

G

A

S

F

Y

P

P

D

C

S

D

H

D

P

E

Q

L

H

S

L

L

G

H

M

M

P
x
L

G

G

M
|
M

H
|
H

G

G

T

T

V

V

A

Y

A

A

V

N

T

Y

S

A

L

V

Q

E

K

H

A

S

D

D

L

L

I

L

A

A

L

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

L

L

D

E

G

A

F

F

A

A

P

S

Y

R

A

A

T

K

I

I

V

V

H

H

A

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

N

N

R

K

A

T

A

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

E

L

V

A

L

L

A

Q

D

G

L

M

I

N

V

K

A

V

V

L

Q

E

N

N

E

R

L

A

D

E

H

E

T

L

P

-

G

-

A

K

C

L

D

D

Y

F

T

G

A

V

W

W

W

R

E

N

Q

E

L

L

G

N

G

V

W

Q

R

K

K

Q

T

K

Y

F

P

P

L

L

G

S

W

F

D

K

P

T

A

F

P

G

D

E

G

A

L

-

L

I

T

P

P

P

Q

Q

Q

Y

V

A

I

I

E

K

R

V

I

L

G

D

Q

E

L

L

V

T

G

D

P

G

D

K

A

A

I

I

Y

I

A

S

A

T

G

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

S

A

Q

Q

F

F

I

Y

Q

N

F

Y

E

K

H

K

P

P

A

R

T

Q

W

W

L

L

N

S

S

S

G
|
G

A

L

G
|
G

T

A

M
|
M

G

G

Y

F

A

G

V

L

P

P

A

A

M

I

G

G

A

A

K

S

A

V

G

A

Q

N

P

P

G

D

T

A

E

I

V

V

W

V

A

D

I

I

D

D

G

G

D
|
D

G

G

C

S

F

F

Q

I

M

M

T

N

N

V

Q

Q

E

E

L

L

V

A

T

T

C

I

A

R

L

V

N

E

N

N

I

L

P

P

I

V

K

K

V

V

A

L

V

L

I

L

N

N

N
|
N

G

Q

S
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

T

D

L

R

F

F

Y

Y

N

K

E

A

R

N

Y

R

S

A

N

H

T

T

V

F

L

L

H

G

S

D

G

-

P

-

G

-

H

-

D

-

G

-

K

-

E

-

Q

-

P

P

A

A

Q

Q

G

E

T

D

R

E

I

I

-

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

A

A

M

C

G

G

C

I

H

P

G

A

L

A

R

R

C

V

E

T

S

K

P

K

D

A

Q

D

L

L

D

R

A

E

V

A

I

I

E

-

Q

Q

A

T

R

M

S

L

L

D

N

T

D

P

A

G

P

P

V

Y

V

L

I

L

D

D

F

V

I

I

V

C

H

P

Q
x
H

D

Q

A

E

M

H

V

V

W

L

P

P

M

M

V

I

A

P

A
x
S

G

G

T

G

N

T

N

F

D

N

E

D

I

V

M

I

A

T

A

E

R

G

D

D

V

G

R

R

active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D307), R292 (= R308), M485 (= M504), W489 (= W508), S568 (≠ A596)
binding flavin-adenine dinucleotide: R161 (= R177), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), Q404 (= Q423), M405 (= M424), G423 (= G442), G424 (= G443)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
44% identity, 94% coverage: 29:610/618 of query aligns to 13:582/582 of 1yhyA

query
sites
1yhyA

S

K

G

G

A

A

Q

D

S

I

L

L

I

V

R

E

S

A

L

L

E

E

A

R

V

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

L
x
Y

P

P

G
|
G

A
|
A

I
x
S

L

M

P

E

A

I

Y

H

D

Q

P

A

L

L

M

T

D

R

S

S

E

S

K

S

L

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

H

F

A

A

T

E

G

G

Y

Y

A

A

Q

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

P

G

I

L

A

A

D

D

A

A

Y

L

M

L

D

D

S

S

V

V

P

P

M

L

V

V

A

A

I

I

T

T

G

G

Q

Q

V

V

A

P

S

R

T

R

S

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

I

I

C

V

G

E

I

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

F

Y

L

L

V

V

T

M

D

D

A

V

A

E

D

D

I

I

P

P

R

R

V

I

I

I

S

E

E

E

A

A

F

F

H

F

I

L

A

A

A

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

V

V

A

P

K
|
K

D

D

A

I

M

Q

Q

Q

K

Q

Q

L

T

A

V

I

F

P

R

N

W

W

P

E

V

Q

E

A

H

M

Q

R

L

L

P

P

G

G

Y

Y

-

M

-

S

R

R

P

M

V

P

T

K

R

P

P

P

H

E

G

D

K

S

Q

H

I

L

R

E

E

Q

A

I

A

V

R

R

L

L

L

I

A

S

Q

E

A

S

R

K

P

P

V

V

L

L

Y

Y

V

V

G
|
G

V

C

L

L

K

N

A

-

R

-

A

S

T

S

A

D

E

E

L

L

K

G

I

R

L

F

A

V

E

E

L

L

T

T

K

G

A

I

P

P

V

V

T

A

T

S

T
|
T

L
|
L

M
|
M

G

G

I

L

G

G

A

S

F

Y

P

P

D

C

S

D

H

D

P

E

Q

L

H

S

L

L

G

H

M

M

P
x
L

G
|
G

M
|
M

H
|
H

G

G

T

T

V

V

A

Y

A

A

V

N

T

Y

S

A

L

V

Q

E

K

H

A

S

D

D

L

L

I

L

A

A

L

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

K

K

L

L

D

E

G

A

F

F

A

A

P

S

Y

R

A

A

T

K

I

I

V

V

H

H

A

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

N

N

R

K

A

T

A

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D

H

V

V

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S

I

V

V

C

G

G

D
|
D

A
x
V

R

K

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L

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L

L

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L

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I

N

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V

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N

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A

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Y

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W

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W

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L

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L

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L

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P

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K

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A

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G

G

V
|
V

G

G

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Q

H

H

Q
|
Q

M
|
M

W

W

A

A

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A

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F

F

I

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W

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L

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|
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A

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|
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I

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D

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|
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F

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M

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N

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N

E

N

N

I

L

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P

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K

K

V

V

A

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V

L

I

L

N

N

N
|
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active site: Y33 (≠ L49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E75), T82 (= T98), F121 (= F137), Q122 (= Q138), E123 (= E139), K171 (= K187), M266 (= M282), V293 (= V309), V400 (= V419), G426 (= G445), M428 (= M447), D453 (= D472), N480 (= N499), H482 (≠ S501), L483 (= L502), M485 (= M504), V486 (= V505), W489 (= W508), H558 (≠ Q586)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D307), R292 (= R308), V486 (= V505), W489 (= W508), S568 (≠ A596)
binding flavin-adenine dinucleotide: R161 (= R177), G222 (= G236), G223 (= G237), G224 (= G238), T246 (= T262), L247 (= L263), M248 (= M264), L264 (≠ P280), G265 (= G281), M266 (= M282), H267 (= H283), G286 (= G302), V287 (≠ A303), R288 (= R304), D290 (= D306), V293 (= V309), D310 (= D326), I311 (= I327), D329 (= D345), V330 (≠ A346), Q404 (= Q423), M405 (= M424), G423 (= G442), G424 (= G443)
binding magnesium ion: D453 (= D472), N480 (= N499), H482 (≠ S501)

Query Sequence

>WP_037572586.1 NCBI__GCF_000744815.1:WP_037572586.1
MTEQAAAHHRGEASADAGAAQPTVAETMSGAQSLIRSLEAVGVDTVFGLPGGAILPAYDP
LMDSEKLRHVLVRHEQGAGHAATGYAQATGKVGVCMATSGPGATNLVTPIADAYMDSVPM
VAITGQVASTSIGTDAFQEADICGITMPITKHNFLVTDAADIPRVISEAFHIAATGRPGP
VLVDVAKDAMQKQTVFRWPVEHQLPGYRPVTRPHGKQIREAARLLAQARRPVLYVGGGVL
KARATAELKILAELTKAPVTTTLMGIGAFPDSHPQHLGMPGMHGTVAAVTSLQKADLIIA
LGARFDDRVTGKLDGFAPYATIVHADIDPAEIGKNRAADVPIVGDAREVLADLIVAVQNE
LDHTPGACDYTAWWEQLGGWRKTYPLGWDPAPDGLLTPQQVIERIGQLVGPDAIYAAGVG
QHQMWASQFIQFEHPATWLNSGGAGTMGYAVPAAMGAKAGQPGTEVWAIDGDGCFQMTNQ
ELVTCALNNIPIKVAVINNGSLGMVRQWQTLFYNERYSNTVLHSGPGHDGKEQPAQGTRI
PDFVKLAEAMGCHGLRCESPDQLDAVIEQARSLNDAPVVIDFIVHQDAMVWPMVAAGTNN
DEIMAARDVRPDFGDNEE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory