SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_037573514.1 NCBI__GCF_000744815.1:WP_037573514.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

8cqxA Ribokinase from t.Sp mutant a92g
34% identity, 98% coverage: 1:364/372 of query aligns to 1:295/300 of 8cqxA

query
sites
8cqxA

M

M

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I

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x
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binding adenosine-5'-diphosphate: N179 (≠ D212), T217 (≠ R249), G219 (= G251), A220 (≠ P252), G222 (= G254), F250 (= F318), N272 (≠ C340), G275 (= G343), A276 (= A344), T279 (≠ V346)
binding magnesium ion: D242 (= D305), T244 (≠ G307), A278 (vs. gap), S287 (≠ A356)

6znxC Ribokinase from thermus species
32% identity, 89% coverage: 34:364/372 of query aligns to 21:260/265 of 6znxC

query
sites
6znxC

R

H

P

P

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x
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binding adenosine-5'-diphosphate: N164 (≠ D212), L184 (= L250), G185 (= G251), F215 (= F318), N237 (≠ C340), G240 (= G343), A241 (= A344), T244 (≠ V346)

4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
27% identity, 99% coverage: 2:371/372 of query aligns to 4:306/306 of 4xckA

query
sites
4xckA

I

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A

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S

active site: A249 (≠ G308), A250 (= A314), G251 (= G315), D252 (= D316)
binding adenosine-5'-diphosphate: T220 (≠ R249), G222 (= G251), S223 (≠ P252), V242 (≠ A301), T247 (≠ A306), A250 (= A314), F254 (= F318), H276 (≠ C340), A279 (≠ G343), V283 (= V346)
binding alpha-D-ribofuranose: N11 (≠ A9), D13 (≠ E11), G39 (= G37), K40 (≠ Q38), N43 (≠ R41), A95 (≠ R102), I107 (≠ A117), I109 (≠ V119), E140 (≠ G150), T248 (≠ G307), D252 (= D316)

1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
28% identity, 68% coverage: 2:254/372 of query aligns to 4:225/305 of 1rk2A

query
sites
1rk2A

I

L

A

V

V

V

V

L

G

G

G

S

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x
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x
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A

I

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x
N

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A

A

A

A

A

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D

L

C

V

A

E

A

A

A

Q

G

R

A

E

L

R

L

I

R

A

D

N

A

A

E

S

V

A

T

L

L

L

L

M

Q

Q

H

L

G
x
E

G

S

A

-

G

-

D

-

R

-

F

-

G

-

A

-

G

-

A

-

R

-

G

-

A

-

L

-

A

-

D

-

P

-

G

-

A

-

P

-

G

-

V

-

P

P

D

L

E

E

A

S

A

V

A

M

A

A

A

A

A

A

R

K

L

I

A

A

G

H

G

Q

T

N

-

K

-

T

-

I

V

V

L

A

L

L

I

N

P

P

G

A

P

P

V

A

G

R

E

E

V

L

P

P

A

D

D

E

L

L

L

L

A

A

M

L

V

V

D

D

V

I

L

I

V

T

P

P

D

N

R

E

D

T

G

E

L

A

A

E

A

K

V

L

V

T

G

G

A

I

A

R

P

V

E

E

D

N

L

D

P

E

D

D

L

-

H

-

A

-

V

A

A

A

D

K

A

A

A

A

R

Q

S

V

L

L

-

H

-

E

R

K

G
|
G

P

I

A
x
R

S

T

V

V

V

L

V

I

R
x
T

L

L

G
|
G

P
x
S

E

R

G

G

binding adenosine-5'-diphosphate: T220 (≠ R249), G222 (= G251), S223 (≠ P252)
binding tetrafluoroaluminate ion: G213 (= G242), R215 (≠ A244)
binding alpha-D-ribofuranose: N11 (≠ A9), D13 (≠ E11), G38 (= G36), G39 (= G37), K40 (≠ Q38), N43 (≠ R41), E140 (≠ G150)

Sites not aligning to the query:

active site: 249, 250, 251, 252
binding adenosine-5'-diphosphate: 250, 251, 276, 279
binding magnesium ion: 246, 282, 285, 291
binding alpha-D-ribofuranose: 252

P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 68% coverage: 2:254/372 of query aligns to 7:228/309 of P0A9J6

query
sites
P0A9J6

I

L

A

V

V

V

V

L

G

G

G

S

V

I

A
x
N

V
x
A

E
x
D

T

H

A

I

L

L

A

N

V

L

P

Q

R

S

L

F

P

P

G

T

V

P

G

G

E

E

T

T

V

V

V

T

G

G

G

N

P

H

A

Y

A

Q

R

V

R

A

P

F

G

G

G
|
G

Q
x
K

G
|
G

L
x
A

R
x
N

Q

Q

A

A

L

V

A

A

A

A

A

G

R

R

L

-

A

-

V

-

G

-

S

-

T

-

G

-

G

-

L

-

G

S

G

G

V

A

R

N

V

I

A

A

I

F

L

I

G

A

R

C

V

T

G

G

A

D

D

D

P

S

D

I

G

G

E

E

L

S

A

V

R

R

S

Q

A

Q

L

L

A

A

E

T

E

D

D

N

V

I

D

D

V

I

A

T

W

-

L

-

M

-

G

-

S

P

V

V

D

S

V

V

M

I

T

K

G

G

Q

E

R

S

-

T

-

G

-

V

-

A

-

L

I

I

T

F

L

V

H

N

P

G

D

E

G

G

E

E

S

N

A

V

G

I

G

G

Q

-

A

-

A

-

A

-

V

I

S

H

P

A

G

G

A

A

N

N

A

A

E

A

L

L

S

S

A

P

E

A

D

L

C

V

A

E

A

A

A

Q

G

R

A

E

L

R

L

I

R

A

D

N

A

A

E

S

V

A

T

L

L

L

L

M

Q

Q

H

L

G
x
E

G

S

A

-

G

-

D

-

R

-

F

-

G

-

A

-

G

-

A

-

R

-

G

-

A

-

L

-

A

-

D

-

P

-

G

-

A

-

P

-

G

-

V

-

P

P

D

L

E

E

A

S

A

V

A

M

A

A

A

A

A

A

R

K

L

I

A

A

G

H

G

Q

T

N

-

K

-

T

-

I

V

V

L

A

L

L

I

N

P

P

G

A

P

P

V

A

G

R

E

E

V

L

P

P

A

D

D

E

L

L

L

L

A

A

M

L

V

V

D

D

V

I

L

I

V

T

P

P

D
x
N

R

E

D

T

G

E

L

A

A

E

A

K

V

L

V

T

G

G

A

I

A

R

P

V

E

E

D

N

L

D

P

E

D

D

L

-

H

-

A

-

V

A

A

A

D

K

A

A

A

A

R

Q

S

V

L

L

-

H

-

E

R

K

G

G

P

I

A

R

S

T

V

V

V

L

V

I

R
x
T

L
|
L

G
|
G

P
x
S

E
x
R

G
|
G

NAD 14:16 (≠ AVE 9:11) binding substrate
GKGAN 42:46 (≠ GQGLR 37:41) binding substrate
E143 (≠ G150) binding substrate
N187 (≠ D212) binding ATP
TLGSRG 223:228 (≠ RLGPEG 249:254) binding ATP

Sites not aligning to the query:

254:255 binding ATP
255 binding substrate
279 binding ATP

1gqtB Activation of ribokinase by monovalent cations (see paper)
28% identity, 68% coverage: 2:254/372 of query aligns to 6:227/307 of 1gqtB

query
sites
1gqtB

I

L

A

V

V

V

V

L

G

G

G

S

V

I

A
x
N

V

A

E
x
D

T

H

A

I

L

L

A

N

V

L

P

Q

R

S

L

F

P

P

G

T

V

P

G

G

E

E

T

T

V

V

V

T

G

G

G

N

P

H

A

Y

A

Q

R

V

R

A

P

F

G

G

G
|
G

Q

K

G

G

L

A

R
x
N

Q

Q

A

A

L

V

A

A

A

A

A

G

R

R

L

-

A

-

V

-

G

-

S

-

T

-

G

-

G

-

L

-

G

S

G

G

V

A

R

N

V

I

A

A

I

F

L

I

G

A

R

C

V

T

G

G

A

D

D

D

P

S

D

I

G

G

E

E

L

S

A

V

R

R

S

Q

A

Q

L

L

A

A

E

T

E

D

D

N

V

I

D

D

V

I

A

T

W

-

L

-

M

-

G

-

S

P

V

V

D

S

V

V

M

I

T

K

G

G

Q

E

R

S

-

T

-

G

-

V

-

A

-

L

I

I

T

F

L

V

H

N

P

G

D

E

G

G

E

E

S

N

A

V

G

I

G

G

Q

-

A

-

A

-

A

-

V

I

S

H

P

A

G

G

A

A

N

N

A

A

E

A

L

L

S

S

A

P

E

A

D

L

C

V

A

E

A

A

A

Q

G

R

A

E

L

R

L

I

R

A

D

N

A

A

E

S

V

A

T

L

L

L

L

M

Q

Q

H

L

G
x
E

G

S

A

-

G

-

D

-

R

-

F

-

G

-

A

-

G

-

A

-

R

-

G

-

A

-

L

-

A

-

D

-

P

-

G

-

A

-

P

-

G

-

V

-

P

P

D

L

E

E

A

S

A

V

A

M

A

A

A

A

A

A

R

K

L

I

A

A

G

H

G

Q

T

N

-

K

-

T

-

I

V

V

L

A

L

L

I

N

P

P

G

A

P

P

V

A

G

R

E

E

V

L

P

P

A

D

D

E

L

L

L

L

A

A

M

L

V

V

D

D

V

I

L

I

V

T

P

P

D
x
N

R

E

D

T

G

E

L

A

A

E

A

K

V

L

V

T

G

G

A

I

A

R

P

V

E

E

D

N

L

D

P

E

D

D

L

-

H

-

A

-

V

A

A

A

D

K

A

A

A

A

R

Q

S

V

L

L

-

H

-

E

R

K

G

G

P

I

A

R

S

T

V

V

V

L

V

I

R
x
T

L

L

G
|
G

P
x
S

E

R

G

G

binding phosphomethylphosphonic acid adenylate ester: N186 (≠ D212), T222 (≠ R249), G224 (= G251), S225 (≠ P252)
binding alpha-D-ribofuranose: N13 (≠ A9), D15 (≠ E11), G41 (= G37), N45 (≠ R41), E142 (≠ G150)

Sites not aligning to the query:

active site: 251, 252, 253, 254
binding phosphomethylphosphonic acid adenylate ester: 252, 253, 278, 281
binding cesium ion: 248, 250, 284, 287, 293
binding alpha-D-ribofuranose: 254

Query Sequence

>WP_037573514.1 NCBI__GCF_000744815.1:WP_037573514.1
MIAVVGGVAVETALAVPRLPGVGETVVGGPAARRPGGQGLRQALAAARLAVGSTGGLGGV
RVAILGRVGADPDGELARSALAEEDVDVAWLMGSVDVMTGQRITLHPDGESAGGQAAAVS
PGANAELSAEDCAAAGALLRDAEVTLLQHGGAGDRFGAGARGALADPGAPGVPDEAAAAA
ARLAGGTVLLIPGPVGEVPADLLAMVDVLVPDRDGLAAVVGAAPEDLPDLHAVADAARSL
RGPASVVVRLGPEGALVVEDGAVLLIPPPAAMPSATLAGTPTATPGTAAGAEAGAGAGAG
AGAVDAGGRWGALAGDAFCAGLAVAVAEREPLHEAARFACAAGALVAHGGVRGLSALPTR
EEVERITSRGSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory