SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_037574635.1 NCBI__GCF_000744815.1:WP_037574635.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
67% identity, 96% coverage: 16:643/652 of query aligns to 12:649/651 of P9WQD1

query
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P9WQD1

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K617 (= K611) modified: N6-acetyllysine; mutation to R: Complete loss of acetyl-coenzyme A synthetase activity.

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
52% identity, 94% coverage: 33:644/652 of query aligns to 28:645/652 of Q8ZKF6

query
sites
Q8ZKF6

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R194 (≠ K196) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T311) binding CoA
N335 (≠ D335) binding CoA
A357 (= A357) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D517) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S523) binding CoA
G524 (= G524) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (≠ N526) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ G586) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K611) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
52% identity, 94% coverage: 33:644/652 of query aligns to 24:639/641 of 2p20A

query
sites
2p20A

Y

Y

A

K

H

Q

A

S

K

I

E

N

D

D

R

P

L

D

A

T

F

F

W

W

A

G

E

E

Q

Q

A

G

R

K

R

I

L

L

S

D

W

W

E

-

V

I

E

T

P

P

T

Y

E

Q

T

K

L

V

D

-

W

-

S

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

Q

E

D

D

G

G

R

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

R

R

V

T

A

A

I

I

H

I

F

W

E

E

G

G

E

D

-

D

P

T

G

S

D

Q

G

S

R

K

T

H

L

I

T

S

Y

Y

A

R

Q

E

L

L

K

H

D

R

E

D

V

V

G

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

E

D

L

L

G

G

I

I

R

K

K

K

G

G

D

D

R

V

V

V

A

A

V

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

T

A

A

A

I

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

A

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

A

P

E

E

A

A

L

V

S

A

A

G

R

R

I

I

Q

I

D

D

A

S

D

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

G

E

G

G

Y

V

R

R

R

A

G

G

K

R

P

S

N

I

A

P

L

L

K

K

A

K

I

N

V

V

D

D

E

D

A

A

V

L

-

K

-

N

P

P

R

N

C

V

P

T

T

S

I

V

E

E

N

H

V

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

Q

S

D

D

V

I

S

D

W

W

G

Q

E

E

-

G

K

R

D

D

K

L

W

W

H

R

E

D

L

L

V

I

E

E

G

K

Q

A

S

S

P

P

E

E

H

H

T

Q

P

P

E

E

A

A

F

M

E

N

A

A

E

E

H

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

T

V

Q

Y

V

A

S

A

Y

T

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

L

Y

K

H

P

P

E

-

T

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

F

L

V

L

Y

Y

G

G

P

P

L

L

S

A

N

C

G

G

A

A

T

T

Q

T

V

L

M

M

Y

F

E

E

G

G

T

V

P

P

D

N

T

W

P

P

H

T

Q

P

G

A

R

R

F

M

W

C

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

L

L

Y

Y

A

T

A

A

P

P

T

T

A

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

D

D

D

K

I

A

P

I

A

E

K

G

F

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

I

E

W

W

Y

Y

R

W

K

K

N

K

I

I

G

G

G

K

E

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

A

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

I

E

E

T

L

K

K

P

A

G

G

A

S

A

A

Q

T

V

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

T

A

V

L

V

V

D

D

D

N

E

E

G

G

R

H

E

P

V

Q

P

E

D

G

G

A

A

T

G

E

G

G

Y

N

L

L

V

V

L

I

T

T

E

D

P

S

W

W

P

P

S

G

M

Q

L

A

R

R

T

T

I

L

W

F

G

G

D

D

D

H

Q

E

R

R

Y

F

I

E

D

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

P

K

G

N

R

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

K

R

D

D

D

E

D

D

G

G

D

Y

I

Y

W

W

L
x
I

L

T

G

G

R
|
R

V

V

D

D

V

V

M

L

L
x
N

V

V

S

S

G

G

H

H

N
x
R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

K

K

V

I

A

A

E

E

S

A

A

A

V

V

G

G

A

I

T

P

D

H

A

A

T

I

T

K

G

G

Q

Q

A

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

-

G

-

T

N

A

H

A

G

S

E

D

E

D

P

G

S

D

P

A

E

L

L

L

Y

A

A

E

E

L

V

R

R

D

N

H

W

V

V

G

A

K

K

T

E

L

I

G

G

P

P

I

L

A

A

K

T

P

P

K

D

R

V

I

L

L

H

T

W

V

T

T

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

A

N

G

R

D

Q

T

I

S

G

N

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

T

G

V

V

M

V

D

E

-

K

L

L

I

L

Q

E

A

E

K

K

active site: T260 (= T263), T412 (= T416), E413 (= E417), N517 (≠ L521), R522 (≠ N526), K605 (= K611)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (≠ L512), R511 (= R515), R522 (≠ N526)

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
52% identity, 94% coverage: 33:644/652 of query aligns to 24:638/640 of 5jrhA

query
sites
5jrhA

Y

Y

A

K

H

Q

A

S

K

I

E

N

D

D

R

P

L

D

A

T

F

F

W

W

A

G

E

E

Q

Q

A

G

R

K

R

I

L

L

S

D

W

W

E

-

V

I

E

T

P

P

T

Y

E

Q

T

K

L

V

D

-

W

-

S

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

Q

E

D

D

G

G

R

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

R

R

V

T

A

A

I

I

H

I

F
x
W

E

E

G

G

E

D

-

D

P

T

G

S

D

Q

G

S

R

K

T

H

L

I

T

S

Y

Y

A

R

Q

E

L

L

K

H

D

R

E

D

V

V

G

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

E

D

L

L

G

G

I

I

R

K

K

K

G

G

D

D

R

V

V

V

A

A

V

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E
|
E

T

A

A

A

I

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

A

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

A

P

E

E

A

A

L

V

S

A

A
x
G

R

R

I

I

Q

I

D

D

A

S

D

S

A

S

K

R

L

L

V

V

I

I

T

T

A

A

D

D

G

E

G

G

Y

V

R
|
R

R

A

G

G

K

R

P

S

N

I

A

P

L

L

K

K

A

K

I

N

V

V

D

D

E

D

A

A

V

L

-

K

-

N

P

P

R

N

C

V

P

T

T

S

I

V

E

E

N

H

V

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

Q

S

D

D

V

I

S

D

W

W

G

Q

E

E

-

G

K

R

D

D

K

L

W

W

H

R

E

D

L

L

V

I

E

E

G

K

Q

A

S

S

P

P

E

E

H

H

T

Q

P

P

E

E

A

A

F

M

E

N

A

A

E

E

H

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

T

V

Q

Y

V

A

S

A

Y

T

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

L

Y

K

H

P

P

E

-

T

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

F

L

V

L

Y

Y

G

G

P

P

L

L

S

A

N

C

G

G

A

A

T

T

Q

T

V

L

M

M

Y

F

E

E

G

G

T

V

P

P

D

N

T

W

P

P

H

T

Q

P

G

A

R

R

F

M

W

C

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

L

L

Y

Y

A

T

A

A

P

P

T

T

A

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

D

D

D

K

I

A

P

I

A

E

K

G

F

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

I

E

W

W

Y

Y

R

W

K

K

N

K

I

I

G

G

G

K

E

E

R

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

A

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

I

E

E

T

L

K

K

P

A

G

G

A

S

A

A

Q

T

V

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

T

A

V

L

V

V

D

D

D

N

E

E

G

G

R

H

E

P

V

Q

P

E

D

G

G

A

A

T

G

E

G

G

Y

N

L

L

V

V

L

I

T

T

E

D

P

S

W

W

P

P

S

G

M

Q

L

A

R

R

T

T

I

L

W

F

G

G

D

D

D

H

Q

E

R

R

Y

F

I

E

D

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

P

K

G

N

R

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

K

R

D

D

D

E

D

D

G

G

D

Y

I

Y

W

W

L
x
I

L

T

G

G

R

R

V

V

D

D

V

V

M

L

L
x
N

V

V

S
|
S

G

G

H

H

N
x
R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

P

P

K

K

V
x
I

A

A

E

E

S

A

A

A

V

V

G

G

A

I

T

P

D

H

A

A

T

I

T

K

G

G

Q

Q

A

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

-

G

-

T

N

A

H

A

G

S

E

D

E

D

P

G

S

D

P

A

E

L

L

L

Y

A

A

E

E

L

V

R

R

D

N

H

W

V

V

G
x
R

K

K

T

E

L

I

G

G

P
|
P

I

L

A

A

K

T

P

P

K

D

R

V

I

L

L

H

T

W

V

T

T

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

E

A

N

G

R

-

Q

D

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

T

G

V

V

M

V

D

E

-

K

L

L

I

L

Q

E

A

E

K

K

active site: T260 (= T263), T412 (= T416), E413 (= E417), N517 (≠ L521), R522 (≠ N526), K605 (= K611)
binding (r,r)-2,3-butanediol: W93 (≠ F100), E140 (= E146), G169 (≠ A175), K266 (= K269), P267 (= P270)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (≠ L512), N517 (≠ L521), R522 (≠ N526)
binding coenzyme a: F159 (= F165), G160 (= G166), G161 (= G167), R187 (= R193), S519 (= S523), R580 (≠ G586), P585 (= P591)
binding magnesium ion: V533 (= V537), H535 (= H539), I538 (≠ V542)

8rplB Amp-forming acetyl-coa synthetase from chloroflexota bacterium with bound acetyl amp (see paper)
50% identity, 95% coverage: 10:630/652 of query aligns to 1:630/630 of 8rplB

query
sites
8rplB

L

L

K

I

E

E

R

R

R

L

F

F

A

P

P

P

R

E

E

D

L

I

A

V

A

K

A

N

A

A

N

N

V

I

T

T

A

A

D

Y

A

M

Y

K

A

S

H

K

A

G

K

F

E

D

D

D

-

Y

-

E

-

A

-

F

-

Y

-

R

-

W

-

S

-

L

-

A

-

N

R

R

L

F

A

E

F

F

W

W

A

N

E

D

Q

M

A

A

R

K

R

E

L

L

S

H

W

W

E

F

V

E

E

P

P

W

T

K

E

S

T

T

L

F

D

E

W

W

S

T

N

D

P

K

P

P

F

F

A

F

K

K

W

W

F

F

Q

T

D

D

G

G

R

K

L

F

N

N

V

I

A

A

Y

Y

N

N

C

C

V

L

D

D

R

R

H

Y

V

M

E

G

A

T

G

P

H

I

G

E

D

D

R

K

V

V

A

A

I

F

H

Y

F

W

E

E

G

G

E

D

P

D

G

G

D

S

G

S

R

R

T

A

L

Y

T

T

Y

Y

A

K

Q

E

L

M

K

Y

D

V

E

L

V

T

G

N

K

R

A

V

A

A

N

K

A

V

L

L

T

Q

E

N

L

Q

G

G

I

V

R

K

K

K

G

G

D

D

R

R

V

V

A

A

V

I

Y

Y

M

M

P

P

M

M

I

I

P

P

E

E

T

M

A

A

I

A

A

S

M

V

L

L

A

A

C

C

A

A

R

R

I

L

G

G

A

A

A

P

H

H

S

M

V

V

F

F

G

G

F

F

S

A

A

A

E

S

A

S

L

L

S

R

A

D

R

R

I

M

Q

N

D

D

A

C

D

D

A

A

K

K

L

V

V

L

I

I

T

T

A

A

D

D

G

G

Y

Y

R

R

R

G

G

G

K

K

P

V

N

I

A

E

L

L

K

K

A

K

I

I

V

A

D

D

E

E

A

A

V

V

P

A

R

E

C

T

P

P

T

T

I

I

E

E

N

K

V

V

L

F

V

V

V

Q

R

R

R

H

T

T

G

G

Q

F

D

E

V

V

S

P

W

M

G

A

E

E

K

G

D

R

K

D

W

V

W

Y

H

L

E

D

L

V

V

L

E

L

G

N

Q

D

S

I

P

P

E

E

H

D

T

T

-

V

-

P

P

C

E

E

A

P

F

V

E

D

A

S

E

E

H

D

P

M

L

L

F

Y

I

I

L

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

V

H

H

T

V

S

H

G

G

Y

Y

L

A

T

V

Q

G

V

C

S

Y

Y

A

T

T

H

T

H

K

A

F

V

V

F

F

D

D

L

I

K

K

P

P

E

-

T

S

D

D

V

V

Y

F

W

W

C

C

T

T

A

A

D

D

I

I

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

F

T

V

I

Y

Y

G

G

P

P

L

M

S

M

N

N

G

A

A

A

T

S

Q

I

V

V

M

L

Y

F

E

E

G

G

T

I

P

P

D

T

T

Y

P

P

H

A

Q

A

G

D

R

R

F

F

W

W

E

S

I

I

I

V

Q

E

K

K

Y

Y

K

K

V

V

S

N

I

I

L

I

Y

Y

A

T

A

A

P

P

T

T

A

A

I

I

R

R

T

S

F

L

M

M

K

R

W

F

G

G

D

E

I

L

P

P

A

A

K

R

F

H

D

D

L

L

S

S

L

L

R

R

V

I

L

L

G

G

S

T

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

I

M

W

W

Y

Y

R

R

K

K

N

N

I

I

G

G

G

H

E

N

R

E

C

L

P

P

I

I

V

M

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E

E

T

T

G

G

A

M

I

I

M

L

I

I

S

S

P

P

L

T

P

P

G

-

V

I

T

L

E

P

T

L

K

K

P

P

G

G

A

S

A

A

Q

S

V

R

P

P

L

L

P

P

G

T

I

I

S

E

A

A

T

D

V

V

D

N

D

K

E

D

G

G

R

K

E

P

V

V

P

G

D

P

G

E

A

H

G

G

Y

F

L

L

V

I

L

I

T

R

E

H

P

P

W

W

P

P

S

A

M

Q

L

M

R

R

T

T

I

I

W

F

G

G

D

D

D

P

Q

D

R

R

Y

Y

I

-

D

K

T

T

Y

Y

W

W

S

E

R

T

F

I

P

P

G

D

R

V

Y

Y

F

F

A

A

G

G

D
|
D

G

A

A

A

K

T

K

M

D

D

D

K

D

M

G

G

D

Y

I

F

W

R

L

I

L

Q

G

G

R
|
R

V

V

D

D

V

V

M

I

L
x
K

V

V

S

S

G

G

H

H

N
x
R

I

L

S

G

T

S

T

M

E

E

V

I

E

E

S

S

A

S

L

L

V
|
V

S

S

H
|
H

P

P

K

A

V

V

A

A

E

E

S

A

A

A

V

A

V

I

G

G

A

K

T

P

D

D

A

E

T

V

T

K

G

G

Q

E

A

H

I

V

V

K

A

V

F

F

V

V

I

I

L

L

R

R

G

N

T

-

A

-

A

G

S

V

D

E

D

P

G

T

D

E

A

S

L

L

L

A

A

V

E

E

L

L

R

K

D

R

H

H

V

V

G

R

K

T

T

L

L

V

G

G

P

P

I

L

A

A

K

T

P

P

K

D

R

E

I

L

L

E

T

F

V

V

T

T

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K

K

I

I

M

M

R

R

L

V

R

R

D

A

V

R

A

E

E

L

N

G

R

E

Q

P

I

V

G

G

D

D

V

I

T

T

binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] ethanoate: G391 (= G387), E392 (= E388), P393 (= P389), T416 (= T412), W417 (= W413), W418 (= W414), Q419 (= Q415), T420 (= T416), D502 (= D500), R517 (= R515), K523 (≠ L521), R528 (≠ N526)
binding magnesium ion: V539 (= V537), H541 (= H539)

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
52% identity, 92% coverage: 43:644/652 of query aligns to 38:645/652 of P27550

query
sites
P27550

F

F

W

W

A

G

E

E

Q

Q

A

G

R

K

R

I

L

L

S

D

W

W

E

-

V

I

E

K

P

P

T

Y

E

Q

T

K

L

V

D

-

W

-

S

K

N

N

P

T

P

S

F

F

A

A

-

P

-

G

-

N

-

V

-

S

-

I

K

K

W

W

F

Y

Q

E

D

D

G

G

R

T

L

L

N

N

V

L

A

A

Y

A

N

N

C

C

V

L

D

D

R

R

H

H

V

L

E

Q

A

E

G

-

H

N

G

G

D

D

R

R

V

T

A

A

I

I

H

I

F

W

E

E

G

G

E

D

-

D

P

A

G

S

D

Q

G

S

R

K

T

H

L

I

T

S

Y

Y

A

K

Q

E

L

L

K

H

D

R

E

D

V

V

G

C

K

R

A

F

A

A

N

N

A

T

L

L

T

L

E

E

L

L

G

G

I

I

R

K

K

K

G

G

D

D

R

V

V

V

A

A

V

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

T

A

A

A

I

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

A

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

A

P

E

E

A

A

L

V

S

A

A

G

R

R

I

I

Q

I

D

D

A

S

D

N

A

S

K

R

L

L

V

V

I

I

T

T

A

S

D

D

G

E

G

G

Y

V

R

R

R

A

G

G

K

R

P

S

N

I

A

P

L

L

K

K

A

K

I

N

V

V

D

D

E

D

A

A

V

L

-

K

-

N

P

P

R

N

C

V

P

T

T

S

I

V

E

E

N

H

V

V

L

V

V

V

V

L

R

K

R

R

T

T

G

G

Q

G

D

K

V

I

S

D

W

W

G

Q

E

E

-

G

K

R

D

D

K

L

W

W

H

H

E

D

L

L

V

V

E

E

G

Q

Q

A

S

S

P

D

E

Q

H

H

T

Q

P

A

E

E

A

E

F

M

E

N

A

A

E

E

H

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

I

V

L

L

H

H

T

T

S

T

G

G

Y

Y

L

L

T

V

Q

Y

V

A

S

A

Y

L

T

T

H

F

H

K

A

Y

V

V

F

F

D

D

L

Y

K

H

P

P

E

-

T

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

F

L

V

L

Y

Y

G

G

P

P

L

L

S

A

N

C

G

G

A

A

T

T

Q

T

V

L

M

M

Y

F

E

E

G

G

T

V

P

P

D

N

T

W

P

P

H

T

Q

P

G

A

R

R

F

M

W

A

E

Q

I

V

Q

D

K

K

Y

H

K

Q

V

V

S

N

I

I

L

L

Y

Y

A

T

A

A

P

P

T

T

A

A

I

I

R

R

T

A

F

L

M

M

K

A

W

E

G

G

D

D

D

K

I

A

P

I

A

E

K

G

F

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

I

E

W

W

Y

Y

R

W

K

K

N

K

I

I

G

G

G

N

E

E

R

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

A

G

I

F

M

M

I

I

S

T

P

P

L

L

P

P

G

G

V

A

T

T

E

E

T

L

K

K

P

A

G

G

A

S

A

A

Q

T

V

R

P

P

L

F

P

F

G

G

I

V

S

Q

A

P

T

A

V

L

V

V

D

D

D

N

E

E

G

G

R

N

E

P

V

L

P

E

D

G

G

A

A

T

G

E

G

G

Y

S

L

L

V

V

L

I

T

T

E

D

P

S

W

W

P

P

S

G

M

Q

L

A

R

R

T

T

I

L

W

F

G

G

D

D

D

H

Q

E

R

R

Y

F

I

E

D

Q

T

T

Y

Y

W

F

S

S

R

T

F

F

P

K

G

N

R

M

Y

Y

F

F

A

S

G

G

D

D

G

G

A

A

K

R

D

D

D

E

D

D

G

G

D

Y

I

Y

W

W

L

I

L

T

G

G

R

R

V

V

D

D

V

V

M

L

L

N

V

V

S

S

G

G

H

H

N

R

I

L

S

G

T

T

T

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

K

K

V

I

A

A

E

E

S

A

A

A

V

V

G

G

A

I

T

P

D

H

A

N

T

I

T

K

G

G

Q

Q

A

A

I

I

V

Y

A

A

F

Y

V

V

I

T

L

L

R

-

G

-

T

N

A

H

A

G

S

E

D

E

D

P

G

S

D

P

A

E

L

L

L

Y

A

A

E

E

L

V

R

R

D

N

H

W

V

V

G

R

K

K

T

E

L

I

G

G

P

P

I

L

A

A

K

T

P

P

K

D

R

V

I

L

L

H

T

W

V

T

T

D

E

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

D

K

V

I

A

A

-

A

-

G

E

D

N

T

R

S

Q

N

I

L

G

G

D

D

V

T

T

S

T

T

L

L

A

A

D

D

S

P

T

G

V

V

M

V

D

E

-

K

L

L

I

L

Q

E

A

E

K

K

K609 (= K611) modified: N6-acetyllysine; by autocatalysis

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
52% identity, 94% coverage: 33:644/652 of query aligns to 23:635/637 of 2p2fA

query
sites
2p2fA

Y

Y

A

K

H

Q

A

S

K

I

E

N

D

D

R

P

L

D

A

T

F

F

W

W

A

G

E

E

Q

Q

A

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active site: T259 (= T263), T411 (= T416), E412 (= E417), N516 (≠ L521), R521 (≠ N526), K604 (= K611)
binding adenosine monophosphate: G382 (= G387), E383 (= E388), P384 (= P389), T407 (= T412), W408 (= W413), W409 (= W414), Q410 (= Q415), T411 (= T416), D495 (= D500), I507 (≠ L512), R510 (= R515), N516 (≠ L521), R521 (≠ N526)
binding coenzyme a: F158 (= F165), R186 (= R193), W304 (= W309), T306 (= T311), P329 (= P334), A352 (= A357), A355 (= A360), S518 (= S523), R579 (≠ G586), P584 (= P591)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
52% identity, 93% coverage: 31:639/652 of query aligns to 25:636/648 of Q89WV5

query
sites
Q89WV5

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