SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_037576987.1 NCBI__GCF_000744815.1:WP_037576987.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

6kvcA Moee5 in complex with udp-glucose and NAD (see paper)
28% identity, 70% coverage: 3:217/309 of query aligns to 3:212/299 of 6kvcA

query
sites
6kvcA

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active site: S109 (≠ E111), S110 (≠ A112), S111 (≠ R113), Y132 (≠ A141), K136 (≠ R145)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ W11), I12 (= I12), D31 (≠ A31), R32 (= R32), R33 (≠ S33), D46 (= D51), L47 (= L52), L65 (= L71), A66 (= A72), A67 (vs. gap), P69 (vs. gap), A107 (≠ S109), S109 (≠ E111), K136 (≠ R145), F161 (= F167), T163 (= T170), V164 (= V171), R170 (= R175), M173 (≠ G178)
binding uridine-5'-diphosphate-glucose: P69 (vs. gap), R72 (≠ K74), S109 (≠ E111), S110 (≠ A112), Y132 (≠ A141), T163 (= T170), M173 (≠ G178), F174 (= F179), R177 (≠ Q182), E189 (≠ G194), I190 (≠ Y195), Y191 (≠ V196), Q196 (≠ T201), R198 (≠ W203)

Sites not aligning to the query:

binding uridine-5'-diphosphate-glucose: 260

H1ZZB0 Aurachin B dehydrogenase; EC 1.1.1.394 from Stigmatella aurantiaca (see paper)
26% identity, 73% coverage: 1:226/309 of query aligns to 1:222/334 of H1ZZB0

query
sites
H1ZZB0

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S111 (= S119) mutation to A: Does not produce aurachin B.
Y139 (≠ A141) mutation to F: Does not produce aurachin B.

6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
30% identity, 72% coverage: 2:224/309 of query aligns to 3:234/308 of 6wj9B

query
sites
6wj9B

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active site: A119 (vs. gap), A120 (≠ N103), A121 (≠ G104), F144 (= F138), K148 (≠ S143)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (≠ W11), I13 (= I12), D32 (≠ A31), D33 (≠ R32), S35 (≠ A34), T36 (≠ A35), G37 (≠ S36), D55 (= D51), A56 (≠ L52), L75 (= L71), A76 (= A72), A77 (vs. gap), S94 (= S86), A117 (vs. gap), A119 (vs. gap), F144 (= F138), K148 (≠ S143), F171 (= F167), F172 (≠ S168), I174 (≠ V171)
binding uridine-diphosphate-n-acetylglucosamine: V81 (≠ F73), N173 (≠ T170), G187 (vs. gap), V188 (≠ L183), F192 (≠ A187), T203 (vs. gap), L204 (≠ Y195), F205 (≠ V196), R212 (≠ W203)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylglucosamine: 248, 271, 274

6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
30% identity, 72% coverage: 2:224/309 of query aligns to 2:233/307 of 6wjaA

query
sites
6wjaA

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active site: A118 (vs. gap), A119 (≠ N103), A120 (≠ G104), F143 (= F138), K147 (≠ S143)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ W11), I12 (= I12), D31 (≠ A31), D32 (≠ R32), S34 (≠ A34), T35 (≠ A35), G36 (≠ S36), A55 (≠ L52), L74 (= L71), A75 (= A72), A76 (vs. gap), S93 (= S86), F143 (= F138), K147 (≠ S143), F170 (= F167), F171 (≠ S168), I173 (≠ V171)
binding uridine-diphosphate-n-acetylgalactosamine: V80 (≠ F73), A120 (≠ G104), N172 (≠ T170), G186 (vs. gap), V187 (≠ L183), F191 (≠ A187), T202 (vs. gap), F204 (≠ V196), R211 (≠ W203)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylgalactosamine: 247, 270, 273

6kv9A Moee5 in complex with udp-glucuronic acid and NAD (see paper)
28% identity, 70% coverage: 3:217/309 of query aligns to 3:213/299 of 6kv9A

query
sites
6kv9A

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S

R

A

V

G

V

G

V

S
x
A

G
x
S

E
x
S

A
x
S

R
x
S

P

V

F

Y

L

G

I

G

A

A

S

D

G

G

L

V

A

M

S

S

G

-

S

-

P

-

G

-

R

-

P

-

L

-

T

-

E

E

E

D

D

D

-

L

P

P

S

R

P

P

F

L

H

S

G

P

A
x
Y

D

G

S

V

M

T

R
x
K

G

L

G

A

S

A

E

E

N

R

L

L

A

A

L

L

S

A

Y

F

A

A

E

A

R

R

G

G

V

D

R

A

P

E

V

L

-

S

-

V

-

G

A

A

L

L

R

R

F
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F

S

F

P

-

T
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T

V
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V

H

Y

-

G

-

P

G

G

M

Q

R
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R

D

P

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D

G
x
M

F
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F

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I

A

S

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x
R

L

L

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K

R

V

A

A

T

R

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R

R

G

G

E

A

P

A

V

G
x
E

Y
x
I

V
x
Y

G

G

D

D

G

G

S

T

T
x
Q

R

L

W
x
R

A

D

A

F

V

T

H

H

R

V

S

S

D

D

A

V

A

V

R

R

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A

V

L

R

M

L

L

active site: S110 (≠ E111), S111 (≠ A112), S112 (≠ R113), Y133 (≠ A141), K137 (≠ R145)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ W11), I12 (= I12), D31 (≠ A31), R32 (= R32), R33 (≠ S33), D47 (= D51), L48 (= L52), L66 (= L71), A67 (= A72), A68 (vs. gap), P70 (vs. gap), C85 (≠ S86), A108 (≠ S109), S109 (≠ G110), K137 (≠ R145), F162 (= F167), T164 (= T170), V165 (= V171), R171 (= R175), M174 (≠ G178)
binding uridine-5'-diphosphate-glucuronic acid: P70 (vs. gap), R73 (≠ K74), S110 (≠ E111), S111 (≠ A112), Y133 (≠ A141), T164 (= T170), R171 (= R175), M174 (≠ G178), F175 (= F179), R178 (≠ Q182), E190 (≠ G194), I191 (≠ Y195), Y192 (≠ V196), Q197 (≠ T201), R199 (≠ W203)

Sites not aligning to the query:

binding uridine-5'-diphosphate-glucuronic acid: 235, 261

1y1pA X-ray structure of aldehyde reductase with NADPH (see paper)
37% identity, 25% coverage: 3:79/309 of query aligns to 14:102/342 of 1y1pA

query
sites
1y1pA

V

V

F

L

V

V

T

T

G
|
G

A

A

T
x
N

G
|
G

W
x
F

I
x
V

G

A

S

S

A

H

V

V

T

V

D

E

E

Q

L

L

L

L

A

E

H

H

G

G

H

Y

Q

K

V

V

V

R

G

G

L

T

A

A

R
|
R

S

S

A

A

A

S

S

K

A

L

A

A

G

N

L

L

Q

Q

A

K

K

R

G

W

A

D

A

A

V

K

H

Y

R

P

G

G

D

R

L

F

D

E

D

T

-

A

-

V

-

V

T

E

D

D

G
x
M

L

L

A

K

K

Q

A

G

A

A

-

Y

-

D

-

E

-

V

-

I

K

K

A

G

A

A

D

A

A

G

V

V

I

A

H

H

L
x
I

A

A

-
x
S

-

V

-

V

-

S

F

F

K

S

H

N

D

K

F

Y

D

D

D

E

binding adenosine monophosphate: G18 (= G7), N20 (≠ T9), G21 (= G10), F22 (≠ W11), R43 (= R32), M71 (≠ G57), S92 (vs. gap)
binding beta-nicotinamide ribose monophosphate: F22 (≠ W11), V23 (≠ I12), I90 (≠ L71)

Sites not aligning to the query:

Q9UUN9 Aldehyde reductase 2; Aldehyde reductase II; ARII; EC 1.1.1.2 from Sporidiobolus salmonicolor (Yeast-like fungus) (Sporobolomyces salmonicolor) (see paper)
37% identity, 25% coverage: 3:79/309 of query aligns to 15:103/343 of Q9UUN9

query
sites
Q9UUN9

V

V

F

L

V

V

T

T

G
|
G

A

A

T

N

G
|
G

W

F

I

V

G
x
A

S

S

A

H

V

V

T

V

D

E

E

Q

L

L

L

L

A

E

H

H

G

G

H

Y

Q

K

V

V

V

R

G

G

L

T

A

A

R

R

S

S

A

A

A

S

S

K

A

L

A

A

G

N

L

L

Q

Q

A

K

K

R

G

W

A

D

A

A

V

K

H

Y

R

P

G

G

D

R

L

F

D

E

D

T

-

A

-

V

-

V

T

E

D

D

G

M

L

L

A

K

K

Q

A

G

A

A

-

Y

-

D

-

E

-

V

-

I

K

K

A

G

A

A

D

A

A

G

V

V

I

A

H

H

L

I

A

A

-

S

-

V

-

V

-

S

F

F

K

S

H

N

D

K

F

Y

D

D

D

E

G19 (= G7) mutation to A: Results in loss of substrate inhibition and of NADPH-dependent reductase activity.
G22 (= G10) mutation to A: Results in loss of substrate inhibition and of NADPH-dependent reductase activity.
A25 (≠ G13) mutation to G: Only active when NADPH is replaced by NADH.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2p5uA Crystal structure of thermus thermophilus hb8 udp-glucose 4-epimerase complex with NAD
40% identity, 20% coverage: 1:63/309 of query aligns to 1:63/311 of 2p5uA

query
sites
2p5uA

M

M

R

R

V

V

F

L

V

V

T

T

G

G

A

G

T

A

G
|
G

W
x
F

I
|
I

G

G

S

S

A

H

V

I

T

V

D

E

E

D

L

L

L

L

A

A

H

R

G

G

H

L

Q

E

V

V

V

A

G

V

L

L

A
x
D

R
x
N

S
x
L

A
|
A

A
x
T

S
x
G

A

K

A

R

G

E

L

N

Q

V

A

P

K

K

G

G

A

V

A

P

V

F

H

F

R

R

G

V

D
|
D

L
|
L

D

R

D

D

T

K

D

E

G

G

L

V

A

E

K

R

A

A

A

F

K

R

binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (≠ W11), I12 (= I12), D31 (≠ A31), N32 (≠ R32), L33 (≠ S33), A34 (= A34), T35 (≠ A35), G36 (≠ S36), D51 (= D51), L52 (= L52)

Sites not aligning to the query:

active site: 117, 119, 120, 143, 147, 181, 185
binding nicotinamide-adenine-dinucleotide: 73, 74, 75, 77, 116, 143, 147, 173

Query Sequence

>WP_037576987.1 NCBI__GCF_000744815.1:WP_037576987.1
MRVFVTGATGWIGSAVTDELLAHGHQVVGLARSAASAAGLQAKGAAVHRGDLDDTDGLAK
AAKAADAVIHLAFKHDFDDYAAAGRSEHQAVLRMLDAIADGGNGSAGGSGEARPFLIASG
LASGSPGRPLTEEDPSPFHGADSMRGGSENLALSYAERGVRPVALRFSPTVHGMRDHGFT
AQLTKVARERGAAGYVGDGSTRWAAVHRSDAARLVRLALEQAPAGSRVHAVAEEGVPSRD
IAAAIGASLGLPTVSVAAEDADAHFGWIGRFFGMDIPASSDRTRKLLDWTPTGPTLLEDI
AAGAYTTAD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory