SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_037577051.1 NCBI__GCF_000744815.1:WP_037577051.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0CH37 NADP-dependent alcohol dehydrogenase C 2; Ms-ADHC 2; EC 1.1.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
64% identity, 100% coverage: 1:348/348 of query aligns to 1:347/349 of P0CH37

query
sites
P0CH37

M

M

S

S

T

T

T

V

S

S

V

-

P

-

A

A

Y

Y

A

A

P

T

A

S

P

A

S

T

A

E

P

P

L

L

E

T

R

K

T

T

I

T

I

I

P

T

R

R

E

A

L

V

R

G

E

P

G

H

D

D

I

V

L

A

I

F

D

D

I

I

K

H

Y

F

A

A

G

G

I

I

C

C

H

H

S

S

D

D

I

I

H

H

Q

T

V

V

R

K

N

A

E

E

W

W

G

G

E

V

G

P

I

N

F

Y

P

P

M

V

V

V

P

P

G

G

H

H

E

E

I

I

A

A

G

G

V

V

A

T

E

E

V

V

G

G

P

S

G

E

V

V

T

T

D

K

F

Y

S

K

V

V

G

G

D

D

R

R

V

V

G

G

V

V

G

G

C

C

M

F

V

V

D

D

S

S

C

C

G

R

E

E

C

C

V

D

N

N

C

C

R

K

K

A

G

G

E

L

E

E

Q

Q

Y

Y

C

C

T

T

-

G

A

T

G

G

N

M

I

V

P

G

T

T

Y

Y

N

N

G

A

I

I

G

D

K

R

D

D

G

G

E

T

P

P

T

T

Y

H

G

G

Y

Y

S

S

T

G

Q

A

V

I

V

V

D

D

H

E

K

N

F

Y

V

V

L

L

R

R

I

I

P

P

H

D

G

S

I

L

S

P

L

L

D

D

E

A

A

A

P

P

L

L

C

C

A

A

G

G

I

I

T

T

Y

Y

S

S

P

P

L

L

R

R

H

H

W

W

N

N

A

A

G

G

P

P

G

G

R

K

Q

K

V

V

A

A

V

V

L

I

G

G

L

L

G

G

L

L

G

G

H

H

L

V

A

A

V

V

K

K

I

L

A

A

H

K

A

A

M

M

G

G

A

A

E

D

V

V

T

T

V

V

L

L

S

S

H

Q

S

S

E

L

K
|
K

K

K

R

M

E

E

D

D

A

G

A

L

K

R

L

L

G

G

A

A

D

S

H

A

Y

Y

V

Y

N

A

T

T

S

S

V

D

E

P

G

E

A

T

L

F

E

D

A

K

L

L

A

A

G

G

K

S

F

F

D

D

V

L

I

I

V

L

N

N

T

T

V

V

S

S

A

A

S

N

L

L

E

D

L

L

D

G

A

A

Y

Y

L

L

A

G

T

L

L

L

T

K

V

L

D

D

G

G

A

A

M

L

A

V

N

E

V

L

G

G

A

L

P

P

E

E

K

H

P

P

V

M

S

E

L

V

N

P

L

A

F

F

S

P

L

L

I

L

G

A

G

Q

R

R

S

N

L

L

A

T

G

G

S

S

M

M

I

I

G

G

I

I

R

P

E

E

T

T

Q

Q

E

E

M

M

L

L

D

D

F

F

C

C

A

A

E

E

H

H

G

D

I

V

G

R

A

P

E

E

I

I

E

E

L

I

I

I

S

T

A

P

D

D

R

Y

I

I

N

N

E

E

A

A

Y

Y

D

E

R

R

V

V

V

L

A

A

S

S

D

D

V

V

R

R

Y

Y

R

R

F

F

V

V

I

I

D

D

T

T

A

A

T

S

I

L

K210 (= K211) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P0CH36 NADP-dependent alcohol dehydrogenase C 1; Ms-ADHC 1; EC 1.1.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
64% identity, 100% coverage: 1:348/348 of query aligns to 1:347/349 of P0CH36

query
sites
P0CH36

M

M

S

S

T

T

T

V

S

S

V

-

P

-

A

A

Y

Y

A

A

P

T

A

S

P

A

S

T

A

E

P

P

L

L

E

T

R

K

T

T

I

T

I

I

P

T

R

R

E

A

L

V

R

G

E

P

G

H

D

D

I

V

L

A

I

F

D

D

I

I

K

H

Y

F

A

A

G

G

I

I

C

C

H

H

S

S

D

D

I

I

H

H

Q

T

V

V

R

K

N

A

E

E

W

W

G

G

E

V

G

P

I

N

F

Y

P

P

M

V

V

V

P

P

G

G

H

H

E

E

I

I

A

A

G

G

V

V

A

T

E

E

V

V

G

G

P

S

G

E

V

V

T

T

D

K

F

Y

S

K

V

V

G

G

D

D

R

R

V

V

G

G

V

V

G

G

C

C

M

F

V

V

D

D

S

S

C

C

G

R

E

E

C

C

V

D

N

N

C

C

R

K

K

A

G

G

E

L

E

E

Q

Q

Y

Y

C

C

T

T

-

G

A

T

G

G

N

M

I

V

P

G

T

T

Y

Y

N

N

G

A

I

I

G

D

K

R

D

D

G

G

E

T

P

P

T

T

Y

H

G

G

Y

Y

S

S

T

G

Q

A

V

I

V

V

D

D

H

E

K

N

F

Y

V

V

L

L

R

R

I

I

P

P

H

D

G

S

I

L

S

P

L

L

D

D

E

A

A

A

P

P

L

L

C

C

A

A

G

G

I

I

T

T

Y

Y

S

S

P

P

L

L

R

R

H

H

W

W

N

N

A

A

G

G

P

P

G

G

R

K

Q

K

V

V

A

A

V

V

L

I

G

G

L

L

G

G

L

L

G

G

H

H

L

V

A

A

V

V

K

K

I

L

A

A

H

K

A

A

M

M

G

G

A

A

E

D

V

V

T

T

V

V

L

L

S

S

H

Q

S

S

E

L

K
|
K

K

K

R

M

E

E

D

D

A

G

A

L

K

R

L

L

G

G

A

A

D

S

H

A

Y

Y

V

Y

N

A

T

T

S

S

V

D

E

P

G

E

A

T

L

F

E

D

A

K

L

L

A

A

G

G

K

S

F

F

D

D

V

L

I

I

V

L

N

N

T

T

V

V

S

S

A

A

S

N

L

L

E

D

L

L

D

G

A

A

Y

Y

L

L

A

G

T

L

L

L

T

K

V

L

D

D

G

G

A

A

M

L

A

V

N

E

V

L

G

G

A

L

P

P

E

E

K

H

P

P

V

M

S

E

L

V

N

P

L

A

F

F

S

P

L

L

I

L

G

A

G

Q

R

R

S

N

L

L

A

T

G

G

S

S

M

M

I

I

G

G

I

I

R

P

E

E

T

T

Q

Q

E

E

M

M

L

L

D

D

F

F

C

C

A

A

E

E

H

H

G

D

I

V

G

R

A

P

E

E

I

I

E

E

L

I

I

I

S

T

A

P

D

D

R

Y

I

I

N

N

E

E

A

A

Y

Y

D

E

R

R

V

V

V

L

A

A

S

S

D

D

V

V

R

R

Y

Y

R

R

F

F

V

V

I

I

D

D

T

T

A

A

T

S

I

L

K210 (= K211) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WQC5 NADP-dependent alcohol dehydrogenase C; EC 1.1.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
61% identity, 99% coverage: 4:348/348 of query aligns to 2:346/346 of P9WQC5

query
sites
P9WQC5

T

S

S

T

V

V

P

A

A

A

Y

Y

A

A

P

M

A

S

P

A

S

T

A

E

P

P

L

L

E

T

R

K

T

T

I

T

I

I

P

T

R

R

E

D

L

P

R

G

E

P

G

H

D

D

I

V

L

A

I

I

D

D

I

I

K

K

Y

F

A

A

G

G

I

I

C

C

H

H

S

S

D

D

I

I

H

H

Q

T

V

V

R

K

N

A

E

E

W

W

G

G

E

Q

G

P

I

N

F

Y

P

P

M

V

V

V

P

P

G

G

H

H

E

E

I

I

A

A

G

G

V

V

A

T

E

A

V

V

G

G

P

S

G

E

V

V

T

T

D

K

F

Y

S

R

V

Q

G

G

D

D

R

R

V

V

G

G

V

V

G

G

C

C

M

F

V

V

D

D

S

S

C

C

G

R

E

E

C

C

V

N

N

S

C

C

R

T

K

R

G

G

E

I

E

E

Q

Q

Y

Y

C

C

T

K

A

P

G

G

N

A

I

N

P

F

T

T

Y

Y

N

N

G

S

I

I

G

G

K

K

D

D

G

G

E

Q

P

P

T

T

Y

Q

G

G

Y

Y

S

S

T

E

Q

A

V

I

V

V

D

D

H

E

K

N

F

Y

V

V

L

L

R

R

I

I

P

P

H

D

G

V

I

L

S

P

L

L

D

D

E

V

A

A

P

P

L

L

C

C

A

A

G

G

I

I

T

T

T

L

Y

Y

S

S

P

P

L

L

R

R

H

H

W

W

N

N

A

A

G

G

P

A

G

N

R

T

Q

R

V

V

A

A

V

I

L

I

G

G

L

L

G

G

L

L

G

G

H

H

L

M

A

G

V

V

K

K

I

L

A

G

H

A

A

A

M

M

G

G

A

A

E

D

V

V

T

T

V

V

L

L

S

S

H

Q

S

S

E

L

K
|
K

K

K

R

M

E

E

D

D

A

G

A

L

K

R

L

L

G

G

A

A

D

K

H

S

Y

Y

V

Y

N

A

T

T

S

A

V

D

E

P

G

D

A

T

L

F

E

R

A

K

L

L

A

R

G

G

K

G

F

F

D

D

V

L

I

I

V

L

N

N

T

T

V

V

S

S

A

A

S

N

L

L

E

D

L

L

D

G

A

Q

Y

Y

L

L

A

N

T

L

L

L

T

D

V

V

D

D

G

G

A

T

M

L

A

V

N

E

V

L

G

G

A

I

P

P

E

E

K

H

P

P

V

M

S

A

L

V

N

P

L

A

F

F

S

A

L

L

I

A

G

L

G

M

R

R

S

S

L

L

A

A

G

G

S

S

M

N

I

I

G

G

I

I

R

A

E

E

T

T

Q

Q

E

E

M

M

L

L

D

N

F

F

C

C

A

A

E

E

H

H

G

G

I

V

G

T

A

P

E

E

I

I

E

E

L

L

I

I

S

E

A

P

D

D

R

Y

I

I

N

N

E

D

A

A

Y

Y

D

E

R

R

V

V

V

L

A

A

S

S

D

D

V

V

R

R

Y

Y

R

R

F

F

V

V

I

I

D

D

T

I

A

S

T

A

I

L

K209 (= K211) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

1uufA Crystal structure of a zinc-type alcohol dehydrogenase-like protein yahk
55% identity, 99% coverage: 5:348/348 of query aligns to 3:338/339 of 1uufA

query
sites
1uufA

S

A

V

V

P

G

A

A

Y

Y

A

S

A

A

P

K

A

Q

P

P

S

L

A

E

P

P

L

M

E

D

R

-

T

-

I

-

I

I

P

T

R

R

E

E

L

P

R

G

E

P

G

N

D

D

I

V

L

K

I

I

D

E

I

I

K

A

Y

Y

A

C

G

G

I

V

C
|
C

H
|
H

S
|
S

D

D

I

L

H
|
H

Q

Q

V

V

R

R

N

S

E

E

W

W

G

A

E

G

G

T

I

V

F

Y

P

P

M

C

V

V

P

P

G

G

H
|
H

E
|
E

I

I

A

V

G

G

V

R

V

V

A

V

E

A

V

V

G

G

P

D

G

Q

V

V

T

E

D

K

F

Y

S

A

V

P

G

G

D

D

R

L

V

V

G

G

V

V

G

G

C

C

M

I

V

V

D

D

S

S

C
|
C

G

K

E

H

C
|
C

V

E

N

E

C
|
C

R

E

K

D

G

G

E

L

E

E

Q

N

Y

Y

C
|
C

T

D

A

H

G

M

N
x
T

I

-

P

G

T

T

Y

Y

N

N

G

S

I

P

G

T

K

P

D

D

G

-

E

E

P

P

-

G

-

H

T

T

Y

L

G

G

Y

Y

S

S

T

Q

Q

Q

V

I

V

V

D

H

H

E

K

R

F

Y

V

V

L

L

R

R

I

I

P

R

H

H

G

P

I

Q

-

E

S

Q

L

L

D

A

E

A

A

V

A

A

P

P

L

L

C
|
C

A

A

G

G

I

I

T
|
T

T

T

Y

Y

S

S

P

P

L

L

R

R

H

H

W

W

N

Q

A

A

G

G

P

P

G

G

R

K

Q

K

V

V

A

G

V

V

L

V

G

G

L

I

G

G

L

L

G

G

H

H

L

M

A

G

V

I

K

K

I

L

A

A

H

H

A

A

M

M

G

G

A

A

E

H

V

V

T

V

V

A

L

F

S

T

H

T

S

S

E

E

K

A

K

K

R

R

E

E

D

A

A

A

A

K

K

A

L

L

G

G

A

A

D

D

H

E

Y

V

V

V

N

N

T

S

S

R

V

N

E

A

G

D

A

E

L

M

E

A

A

A

L

H

A

L

G

K

K

S

F

F

D

D

V

F

I

I

V

L

N

N

T

T

V

V

S

A

A

A

S

P

L

H

E

N

L

L

D

D

A

D

Y

F

L

T

A

T

T

L

L

L

T

K

V

R

D

D

G

G

A

T

M

M

A

T

N

L

V

V

G

G

A

A

P

P

E

E

K

-

P

-

V

-

S

-

L

-

N

-

L

V

F

F

S

N

L

L

I

I

G

M

G

K

R

R

S

A

L

I

A

A

G

G

S

S

M

M

I

I

G

G

I

I

R

P

E

E

T

T

Q

Q

E

E

M

M

L

L

D

D

F

F

C

C

A

A

E

E

H

H

G

G

I

I

G

V

A

A

E

D

I

I

E

E

L

M

I

I

S

R

A

A

D

D

R

Q

I

I

N

N

E

E

A

A

Y

Y

D

E

R

R

V

M

V

L

A

R

S

G

D

D

V

V

R

K

Y

Y

R
|
R

F

F

V

V

I

I

D

D

T

N

A

R

T

T

I

L

active site: C38 (= C43), H39 (= H44), S40 (= S45), H43 (= H48), H60 (= H65), E61 (= E66), C91 (= C96), C94 (= C99), C97 (= C102), C105 (= C110), T109 (≠ N114), C156 (= C160), T160 (= T164), R330 (= R340)
binding zinc ion: C38 (= C43), H60 (= H65), C91 (= C96), C94 (= C99), C97 (= C102), C105 (= C110), C156 (= C160)

Sites not aligning to the query:

binding zinc ion: 339

1yqdA Sinapyl alcohol dehydrogenase complexed with NADP+ (see paper)
48% identity, 97% coverage: 9:344/348 of query aligns to 13:349/359 of 1yqdA

query
sites
1yqdA

Y

W

A

A

P

R

A

D

P

Q

S

S

A

G

P

H

L

L

E

S

R

P

T

F

I

N

I

F

P

S

R

R

E

A

L

T

R

G

E

E

G

E

D

D

I

V

L

R

I

F

D

K

I

V

K

L

Y

Y

A

C

G

G

I

V

C
|
C

H
|
H

S
|
S

D

D

I

L

H
|
H

Q

S

V

I

R

K

N

N

E

D

W

W

G

G

E

F

G

S

I

M

F

Y

P

P

M

L

V

V

P

P

G

G

H
|
H

E
|
E

I

I

A

V

G

G

V

E

V

V

A

T

E

E

V

V

G

G

P

S

G

K

V

V

T

K

D

K

F

V

S

N

V

V

G

G

D

D

R

K

V

V

G

G

V

V

G

G

C

C

M

L

V

V

D

G

S

A

C
|
C

G

H

E

S

C
|
C

V

E

N

S

C
|
C

R

A

K

N

G

D

E

L

E

E

Q

N

Y

Y

C
|
C

T

P

A

K

G

-

N

M

I
|
I

P

L

T

T

Y

Y

N

A

G

S

I

I

G

Y

K

H

D

D

G

G

E

T

P

I

T

T

Y

Y

G

G

Y

Y

S

S

T

N

Q

H

V

M

V

V

V

A

D

N

H

E

K

R

F

Y

V

I

L

I

R

R

I

F

P

P

H

D

G

N

I

M

S

P

L

L

D

D

E

G

A

G

A

A

P

P

L

L

C
|
C

A

A

G

G

I

I

T
|
T

T

V

Y

Y

S

S

P

P

L

L

R

K

H

Y

W

F

N

G

A

L

G

D

-

E

P

P

G

G

R

K

Q

H

V

I

A

G

V

I

L

V

G
|
G

L
|
L

G
|
G

L
|
L

G

G

H

H

L

V

A

A

V

V

K

K

I

F

A

A

H

K

A

A

M

F

G

G

A

S

E

K

V

V

T

T

V

V

L

I

S
|
S

H
x
T

S
|
S

E

P

K

S

K
|
K

R

K

E

E

D

E

A

A

A

L

K

K

-

N

L

F

G

G

A

A

D

D

H

S

Y

F

V

L

N

V

T

S

S

R

V

D

E

Q

G

E

A

Q

L

M

E

Q

A

A

L

A

A

A

G

G

K

T

F

L

D

D

V

G

I

I

V

I

N

D

T
|
T

V
|
V

S
|
S

A

A

S

V

L

H

E

P

L

L

D

L

A

P

Y

L

L

F

A

G

T

L

L

L

T

K

V

S

D

H

G

G

A

K

M

L

A

I

N

L

V
|
V

G
|
G

A
|
A

P

P

E

E

K

K

P

P

V

L

S

E

L

L

N

P

L

A

F

F

S

S

L

L

I

I

G

A

G

G

R

R

R

K

S

I

L

V

A

A

G

G

S

S

M
x
G

I
|
I

G

G

I

M

R

K

E

E

T

T

Q

Q

E

E

M

M

L

I

D

D

F

F

C

A

A

A

E

K

H

H

G

N

I

I

G

T

A

A

E

D

I

I

E

E

L

V

I

I

S

S

A

T

D

D

R

Y

I

L

N

N

E

T

A

A

Y

M

D

E

R

R

V

L

V

A

A

K

S
x
N

D

D

V

V

R

R

Y

Y

R
|
R

F

F

V

V

I

I

D

D

active site: C47 (= C43), H48 (= H44), S49 (= S45), H52 (= H48), H69 (= H65), E70 (= E66), C100 (= C96), C103 (= C99), C106 (= C102), C114 (= C110), I118 (= I115), C163 (= C160), T167 (= T164), R345 (= R340)
binding nadp nicotinamide-adenine-dinucleotide phosphate: C47 (= C43), H48 (= H44), S49 (= S45), H52 (= H48), C163 (= C160), T167 (= T164), G188 (= G184), L189 (= L185), G190 (= G186), G191 (= G187), L192 (= L188), S211 (= S207), T212 (≠ H208), S213 (= S209), K216 (= K212), T251 (= T246), V252 (= V247), S253 (= S248), V274 (= V269), G275 (= G270), A276 (= A271), G299 (≠ M294), I300 (= I295), N340 (≠ S335), R345 (= R340)
binding zinc ion: C47 (= C43), H69 (= H65), C100 (= C96), C103 (= C99), C106 (= C102), C114 (= C110), C163 (= C160)

3twoB The crystal structure of cad from helicobacter pylori complexed with NADP(h) (see paper)
47% identity, 90% coverage: 32:344/348 of query aligns to 31:342/348 of 3twoB

query
sites
3twoB

D

D

I

V

L

L

I

I

D

D

I

I

K

L

Y

Y

A

A

G

G

I

I

C
|
C

H
|
H

S
|
S

D

D

I

I

H
|
H

Q

S

V

A

R

Y

N

S

E

E

W

W

G

K

E

E

G

G

I

I

F

Y

P

P

M

M

V

I

P

P

G

G

H
|
H

E
|
E

I

I

A

A

G

G

V

I

A

K

E

E

V

V

G

G

P

K

G

G

V

V

T

K

D

K

F

F

S

K

V

I

G

G

D

D

R

V

V

V

G

G

V

V

G

G

C

C

M

F

V

V

D

N

S

S

C
|
C

G

K

E

A

C
|
C

V

K

N

P

C
|
C

R

K

K

E

G

H

E

Q

E

E

Q

Q

Y

F

C
|
C

T

T

A

K

G

-

N

V

I
x
V

P

F

T

T

Y

Y

N

D

G

C

I

L

G

D

K

S

-

F

-

H

D

D

G

N

E

E

P

P

T

H

Y

M

G

G

Y

Y

S

S

T

N

Q

N

V

I

V

V

D

D

H

E

K

N

F

Y

V

V

L

I

R

S

I

V

P

D

H

K

G

N

I

A

S

P

L

L

D

E

E

K

A

V

A

A

P

P

L

L

C
|
C

A

A

G

G

I

I

T
|
T

T

T

Y

Y

S

S

P

P

L

L

R

K

H

F

W

S

N

K

A

V

G

T

P

K

G

G

R

T

Q

K

V

V

A

G

V

V

L

A

G
|
G

L
x
F

G
|
G

L
|
L

G

G

H

S

L

M

A

A

V

V

K

K

I

Y

A

A

H

V

A

A

M

M

G

G

A

A

E

E

V

V

T

S

V

V

L

F

S

A

H
x
R

S

N

E

E

K

H

K

K

R

K

E

Q

D

D

A

A

A

L

K

S

L

M

G

G

A

V

D

K

H

H

Y

F

V

Y

N

T

T

D

S

P

V

K

E

Q

G

C

A

K

L

E

E

E

A

-

L

-

A

-

G

-

K

-

F

L

D

D

V

F

I

I

V

I

N

S

T
|
T

V
x
I

S
x
P

A
x
T

S

H

L

Y

E

D

L

L

D

K

A

D

Y

Y

L

L

A

K

T

L

L

L

T

T

V

Y

D

N

G

G

A

D

M

L

A

A

N

L

V
|
V

G
|
G

A
x
L

P

P

E

P

K

V

P

E

V

V

S

A

-

P

-

V

L

L

N

S

L

V

F

F

S

D

L

F

I

I

G

H

-

L

G

G

R

N

R

R

S

K

L

V

A

Y

G

G

S

S

M
x
L

I
|
I

G

G

I

I

R

K

E

E

T

T

Q

Q

E

E

M

M

L

V

D

D

F

F

C

S

A

I

E

K

H

H

G

N

I

I

G

Y

A

P

E

E

I

I

E

D

L

L

I

I

S

L

A

G

D

K

R

D

I

I

N

D

E

T

A

A

Y

Y

D

H

R

N

V
x
L

V

T

A

H

S
x
G

D

K

V

A

R

K

Y

F

R
|
R

F

Y

V

V

I

I

D

D

active site: C42 (= C43), H43 (= H44), S44 (= S45), H47 (= H48), H64 (= H65), E65 (= E66), C95 (= C96), C98 (= C99), C101 (= C102), C109 (= C110), V113 (≠ I115), C160 (= C160), T164 (= T164), R338 (= R340)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: C42 (= C43), H43 (= H44), S44 (= S45), H47 (= H48), C160 (= C160), T164 (= T164), G184 (= G184), F185 (≠ L185), G186 (= G186), G187 (= G187), L188 (= L188), R208 (≠ H208), T241 (= T246), I242 (≠ V247), P243 (≠ S248), T244 (≠ A249), V264 (= V269), G265 (= G270), L266 (≠ A271), L292 (≠ M294), I293 (= I295), L330 (≠ V332), G333 (≠ S335), R338 (= R340)
binding zinc ion: C42 (= C43), H64 (= H65), C95 (= C96), C98 (= C99), C101 (= C102), C109 (= C110), C160 (= C160)

5z0cA Nerol dehydrogenase from persicaria minor (see paper)
48% identity, 98% coverage: 3:344/348 of query aligns to 4:346/367 of 5z0cA

query
sites
5z0cA

T

T

T

V

S

K

V

A

P

V

A

G

Y

W

A

A

P

R

A

D

P

P

S

S

A

G

P

H

L

L

E

S

R

P

T

F

I

T

I

F

P

S

R

R

E

A

L

T

R

G

E

E

G

L

D

D

I

V

L

T

I

F

D

K

I

V

K

L

Y

Y

A

C

G

G

I

I

C
|
C

H

H

S
|
S

D

D

I

L

H
|
H

Q

Y

V

I

R

K

N

N

E

E

W

W

G

S

E

N

G

T

I

I

F

Y

P

P

M

A

V

L

P

P

G

G

H
|
H

E

E

I

I

A

V

G

G

V

E

V

V

A

T

E

E

V

V

G

G

P

S

G

K

V

V

T

N

D

K

F

F

S

K

V

V

G

G

D

D

R

K

V

V

G

G

V

V

G

G

C

C

M

I

V

V

D

G

S

S

C
|
C

G

H

E

S

C
|
C

V

P

N

N

C
|
C

R

N

K

N

G

H

E

L

E

E

Q

N

Y

Y

C
|
C

T

-

A

P

G

N

N

R

I

I

P

L

T

T

Y

F

N

G

G

S

I

R

G

Y

K

Y

D

D

G

G

E

T

P

L

T

N

Y

H

G

G

Y

Y

S

S

T

D

Q

L

V

M

V

V

D

Q

H

E

K

H

F

F

V

A

L

V

R

R

I

I

P

P

H

D

G

A

I

L

S

P

L

L

D

D

E

S

A

A

P

P

L

L

C
|
C

A

A

G

G

I

I

T

T

T

V

Y

Y

S

S

P

P

L

L

R

R

H

Y

W

Y

N

G

A

L

-

D

G

K

P

P

G

G

R

L

Q

H

V

V

A

G

V

V

L

V

G

G

L

L

G

G

L

L

G

G

H

H

L

M

A

A

V

V

K

K

I

F

A

A

H

K

A

A

M

F

G

G

A

V

E

K

V

V

T

T

V

V

L

V

S

S

H

T

S

S

E

P

K

A

K

K

R

K

E

E

D

D

A

A

-

I

A

S

K

G

L

L

G

G

A

A

D

H

H

S

Y

F

V

I

N

L

T

S

S

T

V

D

E

A

G

E

A

Q

L

M

E

Q

A

A

L

A

A

V

G

G

K

T

F

M

D

D

V

G

I

I

V

I

N

D

T

T

V

V

S

S

A

A

S

S

L

H

E

P

L

L

D

P

A

P

Y

L

L

I

A

S

T

L

L

L

T

K

V

S

D

H

G

G

A

K

M

L

A

V

N

M

V

V

G

G

A

D

P

P

E

P

K

K

P

P

V

L

S

E

L

L

N

P

L

V

F

F

S

P

L

L

I

L

G

L

G

G

R

R

R

K

S

M

L

V

A

A

G

G

S

S

M

A

I

I

G

G

I

M

R

K

E

E

T

T

Q

Q

E

E

M

M

L

I

D

D

F

F

C

A

A

A

E

K

H

E

G

G

I

V

G

R

A

A

E

D

I

V

E

E

L

V

I

I

S

P

A

M

D

D

R

Y

I

V

N

N

E

T

A

A

Y

M

D

Q

R

R

V

V

V

S

A

K

S

S

D

D

V

V

R

K

Y

Y

R

R

F

F

V

V

I

I

D

D

active site: C44 (= C43), S46 (= S45), H49 (= H48), H66 (= H65), C160 (= C160)
binding zinc ion: C44 (= C43), H66 (= H65), C97 (= C96), C100 (= C99), C103 (= C102), C111 (= C110), C160 (= C160)

6k3gB Crystal structure of 10-hydroxygeraniol dehydrogenase from cantharanthus roseus in complex with NADP+ (see paper)
50% identity, 97% coverage: 9:347/348 of query aligns to 13:352/356 of 6k3gB

query
sites
6k3gB

Y

W

A

A

P

R

A

D

P

T

S

S

A

G

P

H

L

L

E

S

R

P

T

F

I

H

I

F

P

S

R

R

E

A

L

T

R

G

E

E

G

H

D

D

I

V

L

Q

I

F

D

K

I

V

K

L

Y

Y

A

C

G

G

I

I

C
|
C

H
|
H

S
|
S

D

D

I

L

H
|
H

Q

M

V

I

R

K

N

N

E

E

W
|
W

G

G

E

F

G

T

I

K

F

Y

P

P

M

I

V

V

P

P

G

G

H
|
H

E

E

I

I

A

V

G

G

V

I

V

V

A

T

E

E

V

V

G

G

P

S

G

K

V

V

T

E

D

K

F

F

S

K

V

V

G

G

D

D

R

K

V

V

G

G

V

V

G

G

C

C

M

L

V

V

D

G

S

S

C
|
C

G

R

E

K

C
|
C

V

D

N

M

C
|
C

R

T

K

K

G

D

E

L

E

E

Q

N

Y

Y

C
|
C

T

-

A

P

G

G

N

Q

I

I

P

L

T

T

Y

Y

N

S

G

A

I

T

G

Y

K

T

D

D

G

G

E

T

P

T

T

T

Y

Y

G

G

Y

Y

S

S

T

D

Q

L

V

M

V

V

V

A

D

D

H

E

K

H

F

F

V

V

L

I

R

R

I

W

P

P

H

E

G

N

I

L

S

P

L

M

D

D

E

I

A

G

A

A

P

P

L

L

C
|
C

A

A

G

G

I

I

T
|
T

T

T

Y

Y

S

S

P

P

L

L

R

R

H

Y

W

F

N

G

A

L

-

D

G

K

P

P

G

G

R

T

Q

H

V

V

A

G

V

V

L

V

G
|
G

L
|
L

G
|
G

L
|
L

G

G

H

H

L

V

A

A

V

V

K

K

I

F

A

A

H

K

A

A

M

F

G

G

A

A

E

K

V

V

T

T

V

V

L

I

S
|
S

H
x
T

S
|
S

E

E

K

S

K
|
K

R

K

E

Q

D

E

A

A

-

L

A

E

K

K

L

L

G

G

A

A

D

D

H

S

Y

F

V

L

N

V

T

S

S

R

V

D

E

P

G

E

A

Q

L

M

E

K

A

A

L

A

A

A

G

A

K

S

F

L

D

D

V

G

I

I

V

I

N

D

T
|
T

V
|
V

S
|
S

A
|
A

S

I

L

H

E

P

L

I

D

M

A

P

Y

L

L

L

A

S

T

I

L

L

T

K

V

S

D

H

G

G

A

K

M

L

A

I

N

L

V
|
V

G
|
G

A
|
A

P

P

E

E

K

K

P

P

V

L

S

E

L

L

N

P

L

S

F

F

S

P

L

L

I

I

G

A

G

G

R

R

R

K

S

I

L

I

A

A

G

G

S

S

M
x
A

I
|
I

G

G

I

L

R

K

E

E

T

T

Q

Q

E

E

M

M

L

I

D

D

F

F

C

A

A

A

E

K

H

H

G

N

I

V

G

L

A

P

E

D

I

V

E

E

L

L

I

V

S

S

A

M

D

D

R

Y

I

V

N

N

E

T

A

A

Y

M

D

E

R

R

V

L

A

K

S

A

D

D

V

V

R

K

Y

Y

R
|
R

F

F

V

V

I

I

D

D

T

V

A

A

T

N

active site: C47 (= C43), S49 (= S45), H52 (= H48), H69 (= H65), C163 (= C160)
binding nadp nicotinamide-adenine-dinucleotide phosphate: H48 (= H44), S49 (= S45), H52 (= H48), W58 (= W54), C163 (= C160), T167 (= T164), G188 (= G184), L189 (= L185), G190 (= G186), G191 (= G187), L192 (= L188), S211 (= S207), T212 (≠ H208), S213 (= S209), K216 (= K212), T251 (= T246), V252 (= V247), S253 (= S248), A254 (= A249), V274 (= V269), G275 (= G270), A276 (= A271), A299 (≠ M294), I300 (= I295), R345 (= R340)
binding zinc ion: C47 (= C43), H69 (= H65), C100 (= C96), C103 (= C99), C106 (= C102), C114 (= C110), C163 (= C160)

7cguA Crystal structure of abhar
48% identity, 97% coverage: 9:344/348 of query aligns to 7:344/352 of 7cguA

query
sites
7cguA

Y

W

A

A

P

M

A

D

P

S

S

S

A

G

P

V

L

L

E

S

R

P

T

F

I

T

I

F

P

S

R

R

E

A

L

T

R

G

E

E

G

E

D

D

I

V

L

R

I

L

D

K

I

V

K

L

Y

Y

A

C

G

G

I

I

C
|
C

H

H

S

S

D

D

I

L

H

G

Q

C

V

I

R

K

N

N

E

E

W

W

G

G

E

W

G

C

I

S

F

Y

P

P

M

L

V

V

P

P

G

G

H
|
H

E
|
E

I

I

A

V

G

G

V

I

V

A

A

T

E

E

V

V

G

G

P

S

G

K

V

V

T

T

D

K

F

F

S

K

V

V

G

G

D

D

R

R

V

V

G

G

V

V

G

G

C

C

M

M

V

V

D

G

S

S

C

C

G

G

E

T

C

C

V

Q

N

N

C

C

R

T

K

Q

G

N

E

Q

E

E

Q

S

Y

Y

C

C

T

P

A

E

G

-

N

V

I

I

P

M

T

T

Y

C

N

A

G

S

I

A

G

Y

K

P

D

D

G

G

E

T

P

P

T

T

Y

Y

G

G

Y

F

S

S

T

N

Q

Q

V

M

V

V

V

A

D

N

H

E

K

K

F

F

V

V

L

I

R

R

I

I

P

P

H

N

G

S

I

L

S

P

L

L

D

D

E

A

A

A

P

P

L

L

C
|
C

A

A

G

G

I

S

T

T

T

V

Y

Y

S

S

P

A

L

M

R

K

H

F

W

Y

N

G

-

L

A

C

G

S

P

Q

G

G

R

L

Q

H

V

L

A

G

V

V

L

V

G

G

L

L

G

G

L

L

G

G

H

H

L

V

A

A

V

V

K

K

I

F

A

A

H

K

A

A

M

F

G

G

A

M

E

K

V

V

T

T

V

V

L

I

S

S

H

T

S

S

E

L

K

G

K

K

R

K

E

E

D

E

A

A

A

I

-

N

K

Q

L

L

G

G

A

A

D

D

H

S

Y

F

-

L

V

I

N

N

T

T

S

D

V

T

E

E

-

Q

-

M

-

Q

G

G

A

A

L

M

E

E

A

V

L

M

A

D

G

G

K

-

F

-

D

-

V

-

I

I

V

I

N

D

T

T

V

V

S

S

A

A

S

L

L

H

E

P

L

I

D

E

A

P

Y

L

L

L

A

G

T

L

L

L

T

K

V

S

-

H

D

Q

G

G

A

K

M

L

A

I

N

I

V

V

G

G

A

L

P

P

E

N

K

K

P

Q

V

P

S

E

L

L

N

P

L

V

F

F

S

S

L

L

I

I

G

N

G

G

R

R

R

K

S

M

L

I

A

G

G

G

S

S

M

A

I

V

G

G

I

V

R

K

E

E

T

T

Q

Q

E

E

M

M

L

I

D

D

F

F

C

A

A

A

E

E

H

H

G

N

I

I

G

T

A

A

E

D

I

I

E

E

L

I

I

V

S

P

A

M

D

D

R

Y

I

V

N

N

E

T

A

A

Y

M

D

E

R

R

V

L

V

E

A

K

S

G

D

D

V

V

R

K

Y

F

R

R

F

F

V

V

I

I

D

D

binding zinc ion: C41 (= C43), H63 (= H65), E64 (= E66), C157 (= C160)

5vktA Cinnamyl alcohol dehydrogenases (sbcad4) from sorghum bicolor (l.) Moench (see paper)
45% identity, 97% coverage: 9:346/348 of query aligns to 6:346/353 of 5vktA

query
sites
5vktA

Y

W

A

A

P

R

A

D

P

A

S

S

A

G

P

H

L

L

E

S

R

P

T

Y

I

S

I

F

P

S

R

A

R

R

E

I

L

Q

R

G

E

D

G

A

D

D

I

V

L

T

I

I

D

K

I

V

K

L

Y

F

A

C

G

G

I

I

C
|
C

H
|
H

S
x
T

D

D

I

L

H
|
H

Q

V

V

I

R

K

N

N

E

E

W

W

G

G

E

N

G

A

I

M

F

Y

P

P

M

V

V

V

P

P

G

G

H
|
H

E
|
E

I

V

A

V

G

G

V

V

A

T

E

D

V

V

G

G

P

H

G

G

V

V

T

T

D

K

F

F

S

K

V

A

G

G

D

D

R

T

V

V

G

G

V

V

G

G

C

Y

M

F

V

V

D

D

S

S

C
|
C

G

R

E

T

C
|
C

V

E

N

S

C
|
C

R

S

K

T

G

G

E

H

E

E

Q

N

Y

Y

C
|
C

T

-

A

P

G

D

N

L

I
x
V

P

L

T

T

Y

S

N

N

G

G

I

V

G

D

-

H

K

H

D

H

G

G

E

A

P

T

T

T

Y

K

G

G

Y

F

S

S

T

D

Q

V

V

L

V

V

D

S

H

Q

K

D

F

F

V

V

L

V

R

R

I

V

P

P

H

E

G

S

I

L

S

P

L

L

D

D

E

G

A

A

P

P

L

L

C
|
C

A

A

G

G

I

V

T
|
T

T

V

Y

Y

S

S

P

P

L

M

R

A

H

Q

W

Y

N

A

A

L

G

N

-

E

P

P

G

G

R

K

Q

H

V

L

A

G

V

V

L

V

G
|
G

L
|
L

G
|
G

L
|
L

G

G

H

H

L

M

A

A

V

V

K

K

I

F

A

A

H

K

A

A

M

F

G

G

A

M

E

T

V

V

T

T

V

V

L

I

S
|
S

H
x
S

S
|
S

E

P

K

G

K
|
K

R

R

E

D

D

E

A

A

-

L

A

G

K

R

L

L

G

G

A

A

D

D

H

A

Y

F

V

L

N

V

T

S

S

H

V

D

E

A

G

A

A

Q

L

M

E

K

A

A

L

A

A

A

G

A

K

T

F

L

D

D

V

G

I

I

V

I

N

D

T
|
T

V
|
V

S
|
S

A
|
A

S

G

L

H

E

Q

L

I

D

V

A

P

Y

L

L

L

A

A

T

L

L

L

T

K

V

P

D

M

G

G

A

Q

M

M

A

V

N

V

V
|
V

G
|
G

A

A

P

P

E

S

K

T

P

P

V

L

S

E

L

L

N

P

L

A

F

Y

S

A

L

I

I

I

G

T

G

G

R

G

R

K

S

R

L

V

A

A

G

G

S
x
N

M
x
G

I

V

G

G

G

S

I

V

R

A

E

D

T

C

Q

Q

E

A

M

M

L

L

D

D

F

F

C

A

A

G

E

E

H

H

G

G

I

V

G

T

A

A

E

D

I

I

E

E

L

V

I

V

S

Q

A

M

D

D

R

Y

I

V

N

N

E

T

A

A

Y

I

D

E

R

R

V

L

V

E

A

K

S
x
N

D

D

V

V

R

R

Y

Y

R
|
R

F

F

V

V

I

I

D

D

T

V

A

A

active site: C40 (= C43), H41 (= H44), T42 (≠ S45), H45 (= H48), H62 (= H65), E63 (= E66), C93 (= C96), C96 (= C99), C99 (= C102), C107 (= C110), V111 (≠ I115), C158 (= C160), T162 (= T164), R340 (= R340)
binding nadp nicotinamide-adenine-dinucleotide phosphate: C40 (= C43), H41 (= H44), T42 (≠ S45), H45 (= H48), C158 (= C160), T162 (= T164), G183 (= G184), L184 (= L185), G185 (= G186), G186 (= G187), L187 (= L188), S206 (= S207), S207 (≠ H208), S208 (= S209), K211 (= K212), T246 (= T246), V247 (= V247), S248 (= S248), A249 (= A249), V269 (= V269), G270 (= G270), N293 (≠ S293), G294 (≠ M294), N335 (≠ S335), R340 (= R340)
binding zinc ion: C40 (= C43), H62 (= H65), C93 (= C96), C96 (= C99), C99 (= C102), C107 (= C110), C158 (= C160)

8a3nB Geissoschizine synthase from catharanthus roseus - binary complex with NADP+ (see paper)
44% identity, 97% coverage: 9:344/348 of query aligns to 10:344/352 of 8a3nB

query
sites
8a3nB

Y

W

A

G

A

A

P

A

A

D

P

A

S

S

A

G

P

V

L

L

E

Q

R

P

T

I

I

K

I

F

P

Y

R

R

E

V

L

P

R

G

E

E

G

R

D

D

I

V

L

K

I

I

D

R

I

V

K

L

Y

Y

A

S

G

G

I

V

C
|
C

H
x
N

S

F

D

D

I

M

H

E

Q

M

V

V

R

R

N

N

E

K

W

W

G

G

E

F

G

T

I

R

F

Y

P

P

M

Y

V

V

P

F

G

G

H
|
H

E
|
E

I

T

A

A

G

G

V

E

V

V

A

V

E

E

V

V

G

G

P

S

G

K

V

V

T

E

D

K

F

F

S

K

V

V

G

G

D

D

R

K

V

V

G

A

V

V

G

G

C

C

M

M

V

V

D

G

S

S

C
|
C

G

G

E

Q

C
|
C

V

Y

N

N

C
|
C

R

Q

K

S

G

G

E

M

E

E

Q

N

Y

Y

C
|
C

T

P

A

E

G

P

N

N

I

M

P

A

T

D

Y

-

N

-

G

-

I

-

G

G

K

S

D

V

G

Y

E

E

P

R

T

S

Y

Y

G

G

Y

C

S

S

T

N

Q

V

V

M

V

V

D

D

H

E

K

K

F

F

V

V

L

L

R

R

I

W

P

P

H

E

G

N

I

L

S

P

L

Q

D

D

E

K

A

G

A

V

P

A

L

L

C
|
C

A

A

G

G

I

V

T
x
V

T

V

Y

Y

S

S

P

P

L

M

R

K

H

H

W

L

N

G

A

L

-

D

G

K

P

P

G

G

R

K

Q

H

V

I

A

G

V

V

L

F

G
|
G

L
|
L

G

G

L
|
L

G

G

H

S

L

V

A

A

V

V

K

K

I

F

A

I

H

K

A

A

M

F

G

G

A

G

E

K

V

A

T

T

V

V

L

I

S
|
S

H
x
T

S

S

E

R

K

R

K
|
K

R

E

E

K

D

E

A

A

-

I

A

E

K

E

L

H

G

G

A

A

D

D

H

A

Y

F

V

V

N

V

T

N

S

T

V

D

E

S

G

E

A

Q

L

L

E

K

A

A

L

L

A

A

G

G

K

T

F

M

D

D

V

G

I

V

V

V

N

D

T
|
T

V

T

-
x
P

S

G

A

G

S

R

L

T

E

P

L

M

D

S

A

L

Y

M

L

L

A

N

T

L

L

L

T

K

V

F

D

D

G

G

A

A

M

V

A

M

N

L

V
|
V

G

G

A
|
A

P

P

E

E

K

S

P

L

V

F

S

E

L

L

N

P

L

A

F

A

S

P

L

L

I

I

G

M

G

G

R

R

R

K

S

K

L

I

A

I

G

G

S

S

M
x
S

I

T

G

G

I

L

R

K

E

E

T

Y

Q

Q

E

E

M

M

L

L

D

D

F

F

C

A

A

A

E

K

H

H

G

N

I

I

G

V

A

C

E

D

I

T

E

E

L

V

I

I

S

G

A

I

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D

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Y

I

L

N

S

E

T

A

A

Y

M

D

E

R

R

V

I

V

K

A

N

S
x
L

D

D

V

V

R

K

Y

Y

R
|
R

F

F

V

A

I

I

D

D

binding nadp nicotinamide-adenine-dinucleotide phosphate: N45 (≠ H44), V161 (≠ T164), G182 (= G184), L183 (= L185), L186 (= L188), S205 (= S207), T206 (≠ H208), K210 (= K212), T245 (= T246), P247 (vs. gap), V269 (= V269), A271 (= A271), S294 (≠ M294), L335 (≠ S335), R340 (= R340)
binding zinc ion: C44 (= C43), H66 (= H65), E67 (= E66), C97 (= C96), C100 (= C99), C103 (= C102), C111 (= C110), C157 (= C160)

O49482 Cinnamyl alcohol dehydrogenase 5; AtCAD5; Cinnamyl alcohol dehydrogenase D; EC 1.1.1.195 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
46% identity, 97% coverage: 9:344/348 of query aligns to 13:349/357 of O49482

query
sites
O49482

Y

W

A

A

P

R

A

D

P

P

S

S

A

G

P

I

L

L

E

S

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Y

I

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I

Y

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T

R

L

R

R

E

E

L

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E

P

G

E

D

D

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I

I

D

R

I

I

K

I

Y

C

A

C

G

G

I

I

C
|
C

H

H

S
x
T

D

D

I

L

H

H

Q

Q

V

T

R

K

N

N

E

D

W

L

G

G

E

M

G

S

I

N

F

Y

P

P

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M

V

V

P

P

G

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H
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H

E
|
E

I

V

A

V

G

G

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E

V

V

A

V

E

E

V

V

G

G

P

S

G

D

V

V

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S

D

K

F

F

S

T

V

V

G

G

D

D

R

I

V

V

G

G

V

V

G

G

C

C

M

L

V

V

D

G

S

C

C
|
C

G

G

E

G

C
|
C

V

S

N

P

C
|
C

R

E

K

R

G

D

E

L

E

E

Q

Q

Y

Y

C
|
C

T

P

A

K

G

-

N

K

I

I

P

W

T

S

Y

Y

N

N

G

D

I

V

G

Y

K

I

D

N

G

G

E

Q

P

P

T

T

Y

Q

G

G

Y

F

S

A

T

K

Q

A

V

T

V

V

D

H

H

Q

K

K

F

F

V

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L

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R

K

I

I

P

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E

G

G

I

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S

A

L

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D

E

E

Q

A

A

P

P

L

L

C
|
C

A

A

G

G

I

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|
T

T

V

Y

Y

S

S

P

P

L

L

R

S

H

H

W

F

N

G

-

L

A

K

G

Q

P

P

G

G

R

L

Q

R

V

G

A

G

V

I

L

L

G
|
G

L
|
L

G
|
G

L
x
V

G
|
G

H

H

L

M

A

G

V

V

K

K

I

I

A

A

H

K

A

A

M

M

G

G

A

H

E

H

V

V

T

T

V

V

L

I

S
|
S

H
x
S

S
|
S

E
x
N

K
|
K

R

R

E

E

D

E

A

A

A

L

K

Q

-

D

L

L

G

G

A

A

D

D

H

D

Y

Y

V

V

N

I

T

G

S

S

V

D

E

Q

G

A

A

K

L

M

E

S

A

E

L

L

A

A

G

D

K

S

F

L

D

D

V

Y

I

V

V

I

N

D

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|
T

V

V

S

P

A

V

S

H

L

H

E

A

L

L

D

E

A

P

Y

Y

L

L

A

S

T

L

L

L

T

K

V

L

D

D

G

G

A

K

M

L

A

I

N

L

V

M

G
|
G

A

V

P

I

E

N

K

N

P

P

V

L

S

Q

L

F

N

L

L

T

F

P

S

L

L

L

I

M

G

L

G

G

R

R

R

K

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V

L

I

A

T

G

G

S
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S

M
x
F

I
|
I

G

G

G

S

I

M

R

K

E

E

T

T

Q

E

E

E

M

M

L

L

D

E

F

F

C

C

A

K

E

E

H

K

G

G

I

L

G

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A

S

E

I

I

I

E

E

L

V

I

V

S

K

A

M

D

D

R

Y

I

V

N

N

E

T

A

A

Y

F

D

E

R

R

V

L

V

E

A

K

S

N

D

D

V

V

R

R

Y

Y

R

R

F

F

V

V

I

V

D

D

C47 (= C43) binding Zn(2+)
T49 (≠ S45) binding NADP(+)
H69 (= H65) binding Zn(2+)
E70 (= E66) binding Zn(2+); mutation to A: Loss of activity.
C100 (= C96) binding Zn(2+)
C103 (= C99) binding Zn(2+)
C106 (= C102) binding Zn(2+)
C114 (= C110) binding Zn(2+)
C163 (= C160) binding Zn(2+)
T167 (= T164) binding NADP(+)
GLGGVG 188:193 (≠ GLGGLG 184:189) binding NADP(+)
SSSNKK 211:216 (≠ SHSEKK 207:212) binding NADP(+)
T251 (= T246) binding NADP(+)
G275 (= G270) binding NADP(+)
SFI 298:300 (≠ SMI 293:295) binding NADP(+)

Q6ZHS4 Cinnamyl alcohol dehydrogenase 2; OsCAD2; Protein GOLD HULL AND INTERNODE 2; EC 1.1.1.195 from Oryza sativa subsp. japonica (Rice) (see paper)
45% identity, 98% coverage: 5:346/348 of query aligns to 9:351/363 of Q6ZHS4

query
sites
Q6ZHS4

S

T

V

V

P

T

A

G

Y

W

A

A

P

R

A

D

P

A

S

S

A

G

P

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L

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I

N

I

Y

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L

R

R

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L

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G

E

P

G

E

D

D

I

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L

V

I

V

D

K

I

V

K

L

Y

Y

A

C

G

G

I

I

C

C

H

H

S

T

D

D

I

I

H

H

Q

Q

V

A

R

K

N

N

E

H

W

L

G

G

E

A

G

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I

K

F

Y

P

P

M

M

V

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P

P

G

G

H

H

E

E

I

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A

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G

G

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E

V

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A

V

E

E

V

V

G

G

P

P

G

E

V

V

T

T

D

K

F

Y

S

S

V

A

G

G

D

D

R

V

V

V

G

G

V

V

G

G

C

V

M

I

V

V

D

G

S

C

C

C

G

R

E

E

C

C

V

H

N

P

C

C

R

K

K

A

G

N

E

V

E

E

Q

Q

Y

Y

C

C

T

N

A

K

G

-

N

R

I

I

P

W

T

S

Y

Y

N

N

G

D

I

V

G

Y

K

T

D

D

G

G

E

R

P

P

T

T

Y

Q

G

G

Y

F

S

A

T

S

Q

A

V

M

V

V

D

D

H

Q

K

K

F

F

V

V

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V

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K

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I

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P

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A

G

G

I

L

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A