SitesBLAST
Comparing WP_038017795.1 NCBI__GCF_000757425.2:WP_038017795.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8gy3B Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
53% identity, 95% coverage: 2:149/156 of query aligns to 4:151/154 of 8gy3B
- binding fe2/s2 (inorganic) cluster: G38 (= G36), C39 (= C37), G40 (= G38), G42 (≠ S40), C44 (= C42), G45 (= G43), C47 (= C45), C59 (= C57), C97 (= C96), C100 (= C99), Q101 (= Q100), C132 (= C130), C134 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q96 (= Q95), C134 (= C132)
P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
38% identity, 95% coverage: 1:148/156 of query aligns to 63:226/229 of P77165
- C99 (= C37) binding [2Fe-2S] cluster
- C104 (= C42) binding [2Fe-2S] cluster
- G105 (= G43) binding [2Fe-2S] cluster
- C107 (= C45) binding [2Fe-2S] cluster
- C119 (= C57) binding [2Fe-2S] cluster
- C158 (= C96) binding [2Fe-2S] cluster
- C161 (= C99) binding [2Fe-2S] cluster
- C208 (= C130) binding [2Fe-2S] cluster
- C210 (= C132) binding [2Fe-2S] cluster
5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
38% identity, 95% coverage: 1:148/156 of query aligns to 12:175/175 of 5g5gA
- binding fe2/s2 (inorganic) cluster: G47 (= G36), C48 (= C37), D49 (≠ G38), G51 (≠ S40), C53 (= C42), G54 (= G43), C56 (= C45), C68 (= C57), C107 (= C96), G108 (= G97), C110 (= C99), C157 (= C130), C159 (= C132)
1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
34% identity, 98% coverage: 3:155/156 of query aligns to 6:161/906 of 1dgjA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y35), G39 (= G36), C40 (= C37), G41 (= G38), G43 (≠ S40), Q44 (= Q41), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding pterin cytosine dinucleotide: Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 391, 427, 503, 507, 535, 869, 870
- binding molybdenum (iv)oxide: 424, 535, 698, 869
- binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
35% identity, 97% coverage: 1:151/156 of query aligns to 5:157/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G36), C41 (= C37), D42 (≠ G38), G44 (≠ S40), C46 (= C42), G47 (= G43), C49 (= C45), C61 (= C57), C101 (= C96), G102 (= G97), C104 (= C99), C136 (= C130), C138 (= C132)
- binding pterin cytosine dinucleotide: Q100 (= Q95), C138 (= C132)
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y35), C40 (= C37), E41 (≠ G38), G43 (≠ S40), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y35), C40 (= C37), E41 (≠ G38), G43 (≠ S40), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y35), C40 (= C37), E41 (≠ G38), G43 (≠ S40), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of 4c7yA
- binding fe2/s2 (inorganic) cluster: C40 (= C37), E41 (≠ G38), G43 (≠ S40), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 535, 539
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
- binding hydrogen peroxide: 696, 697, 869
3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of 3fc4A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y35), C40 (= C37), E41 (≠ G38), G43 (≠ S40), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 1,2-ethanediol: 535, 622, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of 3fahA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y35), C40 (= C37), E41 (≠ G38), G43 (≠ S40), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of 1sijA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y35), C40 (= C37), E41 (≠ G38), G43 (≠ S40), C45 (= C42), G46 (= G43), C48 (= C45), R58 (= R55), C60 (= C57), Q99 (= Q95), C100 (= C96), G101 (= G97), C103 (= C99), C137 (= C130), C139 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C139 (= C132)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding arsenite: 535, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Q46509 Aldehyde oxidoreductase; Molybdenum iron sulfur protein; EC 1.2.99.7 from Megalodesulfovibrio gigas (Desulfovibrio gigas) (see paper)
36% identity, 93% coverage: 3:147/156 of query aligns to 6:154/907 of Q46509
- C40 (= C37) binding [2Fe-2S] cluster
- C45 (= C42) binding [2Fe-2S] cluster
- C48 (= C45) binding [2Fe-2S] cluster
- C60 (= C57) binding [2Fe-2S] cluster
- C100 (= C96) binding [2Fe-2S] cluster
- C103 (= C99) binding [2Fe-2S] cluster
- C137 (= C130) binding [2Fe-2S] cluster
- C139 (= C132) binding [2Fe-2S] cluster
1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
34% identity, 96% coverage: 1:150/156 of query aligns to 5:155/161 of 1sb3C
- binding fe2/s2 (inorganic) cluster: Q39 (≠ Y35), C41 (= C37), G44 (≠ S40), C46 (= C42), G47 (= G43), C49 (= C45), C61 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C135 (= C130), C137 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q95), C137 (= C132)
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
37% identity, 94% coverage: 3:148/156 of query aligns to 14:159/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C37), S50 (= S40), C52 (= C42), G53 (= G43), C55 (= C45), C67 (= C57), C106 (= C96), G107 (= G97), C109 (= C99), C141 (= C130), C143 (= C132)
- binding pterin cytosine dinucleotide: Q105 (= Q95), C143 (= C132)
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
33% identity, 92% coverage: 5:148/156 of query aligns to 8:152/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ Y35), C40 (= C37), S43 (= S40), C45 (= C42), G46 (= G43), C48 (= C45), C60 (= C57), C99 (= C96), G100 (= G97), C102 (= C99), C134 (= C130), C136 (= C132)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q95), C136 (= C132)
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
33% identity, 92% coverage: 5:148/156 of query aligns to 9:153/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C37), S44 (= S40), H45 (≠ Q41), C46 (= C42), G47 (= G43), C49 (= C45), C61 (= C57), C100 (= C96), G101 (= G97), C103 (= C99), C135 (= C130), C137 (= C132)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
35% identity, 92% coverage: 1:143/156 of query aligns to 6:149/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ L39), G45 (≠ S40), E46 (≠ Q41)
- binding fe2/s2 (inorganic) cluster: E40 (≠ Y35), C42 (= C37), S43 (≠ G38), G45 (≠ S40), C47 (= C42), G48 (= G43), C50 (= C45), C62 (= C57), Q100 (= Q95), C101 (= C96), G102 (= G97), C104 (= C99), C136 (= C130), C138 (= C132)
- binding pterin cytosine dinucleotide: Q100 (= Q95), C138 (= C132)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
35% identity, 92% coverage: 1:143/156 of query aligns to 6:149/157 of Q0QLF3
- C42 (= C37) binding [2Fe-2S] cluster
- C47 (= C42) binding [2Fe-2S] cluster
- C50 (= C45) binding [2Fe-2S] cluster
- C62 (= C57) binding [2Fe-2S] cluster
- C101 (= C96) binding [2Fe-2S] cluster
- C104 (= C99) binding [2Fe-2S] cluster
- C136 (= C130) binding [2Fe-2S] cluster
- C138 (= C132) binding [2Fe-2S] cluster
P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
38% identity, 95% coverage: 1:148/156 of query aligns to 6:155/166 of P19921
- C42 (= C37) binding [2Fe-2S] cluster
- C47 (= C42) binding [2Fe-2S] cluster
- C50 (= C45) binding [2Fe-2S] cluster
- C62 (= C57) binding [2Fe-2S] cluster
- C102 (= C96) binding [2Fe-2S] cluster
- C105 (= C99) binding [2Fe-2S] cluster
- C137 (= C130) binding [2Fe-2S] cluster
- C139 (= C132) binding [2Fe-2S] cluster
Query Sequence
>WP_038017795.1 NCBI__GCF_000757425.2:WP_038017795.1
MELLINNRTVQIDVEGDTPLLWVIRDDLGMTGTKYGCGLSQCGACSVMVDGQLIRSCVTP
IDKLEGKHITTIEAIDTDDLGKRVVAAWVKHQVPQCGYCQSGQVIAATALLKQVAHPTEE
EIAAAMVNLCRCGTYNAISAAIHEVASEEVRNEKTS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory