SitesBLAST
Comparing WP_038019026.1 NCBI__GCF_000757425.2:WP_038019026.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8jwmB Crystal structure of akrtyl-NADP-tylosin complex (see paper)
34% identity, 98% coverage: 6:315/317 of query aligns to 6:318/331 of 8jwmB
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), M21 (≠ N21), D48 (= D48), Y53 (= Y53), N161 (= N159), Q186 (= Q184), W214 (≠ Y213), S215 (≠ Y214), P216 (≠ S215), L217 (= L216), G219 (≠ S218), G220 (= G219), R235 (≠ S234), R236 (≠ K235), A237 (≠ R236), I287 (= I284), G289 (≠ S286), R291 (≠ T288), Q295 (= Q292), S298 (= S295)
- binding tylosin: Y53 (= Y53), W55 (= W56), H130 (= H128), C192 (≠ Y190), E193 (≠ D191), R195 (≠ S193), G240 (= G239), R241 (vs. gap), Q250 (≠ R247), E253 (≠ K250), Q254 (≠ I251), R257 (≠ T254), E317 (≠ T314), I318 (≠ V315)
Sites not aligning to the query:
8jwoL Crystal structure of akrtyl-tylosin complex (see paper)
34% identity, 98% coverage: 6:315/317 of query aligns to 6:298/311 of 8jwoL
Sites not aligning to the query:
8jwkD The second purified state crystal structure of akrtyl (see paper)
35% identity, 98% coverage: 6:315/317 of query aligns to 6:301/314 of 8jwkD
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), M21 (≠ N21), D48 (= D48), Y53 (= Y53), H130 (= H128), Q186 (= Q184), W214 (≠ Y213), S215 (≠ Y214), P216 (≠ S215), L217 (= L216), I270 (= I284), G272 (≠ S286), R274 (≠ T288), Q278 (= Q292), S281 (= S295)
8jwkH The second purified state crystal structure of akrtyl (see paper)
34% identity, 98% coverage: 6:315/317 of query aligns to 6:294/307 of 8jwkH
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), M21 (≠ N21), D48 (= D48), Y53 (= Y53), H129 (= H128), N160 (= N159), Q185 (= Q184), W213 (≠ Y213), S214 (≠ Y214), P215 (≠ S215), L216 (= L216), G218 (≠ S218), I263 (= I284), G265 (≠ S286), R267 (≠ T288), Q271 (= Q292), S274 (= S295)
P46336 Aldo-keto reductase IolS; AKR11A; Vegetative protein 147; VEG147; EC 1.1.1.- from Bacillus subtilis (strain 168) (see paper)
30% identity, 99% coverage: 1:315/317 of query aligns to 1:308/310 of P46336
6ow0A Crystal structure of mithramycin 3-side chain keto-reductase mtmw in complex with NAD+ and peg (see paper)
31% identity, 99% coverage: 1:315/317 of query aligns to 1:315/323 of 6ow0A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), L21 (≠ V22), D49 (= D48), Y54 (= Y53), S151 (= S158), Y204 (= Y213), F205 (≠ Y214), L207 (= L216), Q209 (≠ S218), G210 (= G219), T213 (= T222), K215 (= K224), R227 (≠ Q228), V284 (≠ I284), G286 (≠ S286), Q292 (= Q292), N296 (≠ F296)
8hw0A The structure of akr6d1
32% identity, 99% coverage: 1:315/317 of query aligns to 1:318/329 of 8hw0A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), W21 (≠ N21), Q27 (≠ T26), D49 (= D48), Y54 (≠ N60), R123 (≠ W129), S152 (= S158), Q178 (= Q184), W207 (≠ Y213), S208 (≠ Y214), P209 (≠ S215), L210 (= L216), S212 (= S218), K218 (= K224), S227 (≠ L231), R228 (≠ Q232), I285 (= I284), G287 (≠ S286), S289 (≠ T288), Q293 (= Q292), D296 (≠ S295), N297 (≠ F296)
1lqaA Tas protein from escherichia coli in complex with NADPH (see paper)
29% identity, 100% coverage: 1:317/317 of query aligns to 1:339/346 of 1lqaA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), M21 (≠ N21), D48 (= D48), Y53 (= Y53), H132 (= H128), N180 (= N159), Q205 (= Q184), Y233 (= Y213), S234 (≠ Y214), L236 (= L216), F238 (≠ S218), G239 (= G219), T242 (= T222), K244 (= K224), A254 (≠ S234), R255 (≠ K235), G308 (≠ S286), T310 (= T288), Q314 (= Q292), N318 (≠ F296)
P0A9T4 Protein tas from Escherichia coli (strain K12) (see paper)
29% identity, 100% coverage: 1:317/317 of query aligns to 1:339/346 of P0A9T4
- 234:244 (vs. 214:224, 55% identical) binding NADP(+)
6ow0B Crystal structure of mithramycin 3-side chain keto-reductase mtmw in complex with NAD+ and peg (see paper)
32% identity, 99% coverage: 1:315/317 of query aligns to 1:291/301 of 6ow0B
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G19 (= G19), L21 (≠ N21), Y50 (= Y53), H117 (= H128), S147 (= S158), Y200 (= Y213), F201 (≠ Y214), L203 (= L216), Q205 (≠ S218), T209 (= T222), Q268 (= Q292), N272 (≠ F296)
1pz0A Structure of NADPH-dependent family 11 aldo-keto reductase akr11a(holo) (see paper)
30% identity, 99% coverage: 3:315/317 of query aligns to 2:307/311 of 1pz0A
- active site: D52 (= D48), Y57 (= Y53), N91 (≠ A91), H124 (= H128)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: H124 (= H128), Q174 (= Q184), Y202 (= Y213), F203 (≠ Y214), P204 (≠ S215), L205 (= L216), S207 (= S218), G208 (= G219), A211 (≠ T222), K213 (= K224)
P80874 Aldo-keto reductase YhdN; AKR11B; General stress protein 69; GSP69; EC 1.1.1.- from Bacillus subtilis (strain 168) (see paper)
33% identity, 94% coverage: 18:315/317 of query aligns to 23:309/331 of P80874
- D52 (= D48) binding NADP(+)
- Q175 (= Q184) binding NADP(+)
- YGSLCR 203:208 (≠ YYSLAS 213:218) binding NADP(+)
- K214 (= K224) binding NADP(+)
- R227 (≠ G237) binding NADP(+)
- GAR 280:282 (≠ SAT 286:288) binding NADP(+)
- Q286 (= Q292) binding NADP(+)
Sites not aligning to the query:
1pz1A Structure of NADPH-dependent family 11 aldo-keto reductase akr11b(holo) (see paper)
33% identity, 94% coverage: 18:315/317 of query aligns to 23:309/333 of 1pz1A
- active site: D52 (= D48), Y57 (= Y53), K90 (vs. gap), Q93 (≠ E96), H125 (= H128)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: Q175 (= Q184), Y203 (= Y213), G204 (≠ Y214), L206 (= L216), R208 (≠ S218), K214 (= K224), G280 (≠ S286), R282 (≠ T288), Q286 (= Q292)
Sites not aligning to the query:
7wf3C Composite map of human kv1.3 channel in apo state with beta subunits (see paper)
29% identity, 94% coverage: 4:302/317 of query aligns to 7:306/328 of 7wf3C