SitesBLAST
Comparing WP_038019118.1 NCBI__GCF_000757425.2:WP_038019118.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1z05A Crystal structure of the rok family transcriptional regulator, homolog of e.Coli mlc protein.
43% identity, 97% coverage: 13:405/406 of query aligns to 1:395/396 of 1z05A
P50456 DNA-binding transcriptional repressor Mlc; Making large colonies protein; Membrane linked control from Escherichia coli (strain K12) (see 4 papers)
40% identity, 99% coverage: 6:405/406 of query aligns to 4:405/406 of P50456
- R52 (= R54) mutation to H: Shows increased expression and forms larger colonies.
- H86 (= H87) mutation to R: Can be bound and inactivated by MtfA.
- F136 (≠ I137) mutation to A: Decreases association with PtsG EIIB domain.
- H247 (= H247) binding Zn(2+)
- C257 (= C257) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-259.; mutation to S: Strongly reduced activity; when associated with S-259.
- C259 (= C259) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-257.; mutation to S: Strongly reduced activity; when associated with S-257.
- C264 (= C264) binding Zn(2+)
- R306 (≠ Q306) mutation to G: Forms dimers but not tetramers; when associated with G-310.
- L310 (= L310) mutation to G: Forms dimers but not tetramers; when associated with G-306.
1z6rA Crystal structure of mlc from escherichia coli (see paper)
39% identity, 96% coverage: 15:405/406 of query aligns to 2:381/382 of 1z6rA
5f7qE Rok repressor lmo0178 from listeria monocytogenes bound to operator (see paper)
29% identity, 82% coverage: 10:343/406 of query aligns to 6:337/396 of 5f7qE
- binding zinc ion: H243 (= H247), C253 (= C257), C255 (= C259), C260 (= C264)
- binding : K8 (≠ I12), K12 (= K16), N15 (= N19), T32 (≠ I36), S43 (≠ A47), T44 (≠ S48), T67 (= T71), G68 (= G72), G68 (= G72), G69 (= G73), G69 (= G73), R70 (= R74), R70 (= R74), R71 (= R75), A72 (= A76), K73 (≠ I77)
Sites not aligning to the query:
5f7rA Rok repressor lmo0178 from listeria monocytogenes bound to inducer (see paper)
28% identity, 64% coverage: 85:343/406 of query aligns to 1:253/306 of 5f7rA
- binding alpha-D-glucopyranose: K7 (≠ R92), E10 (≠ R95), G70 (= G156), N110 (≠ D195), N110 (≠ D195), S134 (≠ R219), V135 (≠ I220), G138 (= G223), L139 (≠ T224), G140 (= G225), E159 (= E244), H162 (= H247), E181 (= E266), E253 (= E343)
- binding zinc ion: H162 (= H247), C172 (= C257), C174 (= C259), C179 (= C264)
Sites not aligning to the query:
3vglA Crystal structure of a rok family glucokinase from streptomyces griseus in complex with glucose and amppnp (see paper)
28% identity, 75% coverage: 88:392/406 of query aligns to 3:311/312 of 3vglA
- binding phosphoaminophosphonic acid-adenylate ester: G9 (= G94), T11 (≠ Q96), K12 (≠ D97), G130 (≠ H221), T131 (≠ R222), G180 (≠ N271), G214 (≠ I297), S218 (≠ C301), G260 (≠ E343), V261 (≠ L344), E264 (= E346)
- binding beta-D-glucopyranose: G65 (= G156), P78 (= P169), N103 (≠ H194), D104 (= D195), L133 (≠ T224), G134 (= G225), E153 (= E244), H156 (= H247), E175 (= E266)
- binding zinc ion: H156 (= H247), C166 (= C257), C168 (= C259), C173 (= C264)
3vgkB Crystal structure of a rok family glucokinase from streptomyces griseus (see paper)
28% identity, 75% coverage: 88:392/406 of query aligns to 3:311/312 of 3vgkB
2qm1B Crystal structure of glucokinase from enterococcus faecalis
25% identity, 74% coverage: 89:390/406 of query aligns to 9:319/325 of 2qm1B
2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
27% identity, 68% coverage: 89:364/406 of query aligns to 6:279/308 of 2yi1A
- binding adenosine-5'-diphosphate: G11 (= G94), T13 (≠ Q96), N14 (≠ D97), R16 (≠ T99), T140 (≠ R222), G189 (≠ N271), L216 (≠ S298), V261 (≠ E343)
- binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G12 (≠ R95), G71 (≠ P155), G72 (= G156), R73 (≠ L157), S84 (≠ M168), T85 (≠ P169), L87 (≠ I171), N112 (≠ H194), D113 (= D195), G139 (≠ H221), T140 (≠ R222), G141 (= G223), I142 (≠ T224), E162 (= E244), H165 (= H247), E184 (= E266)
- binding calcium ion: N112 (≠ H194), N115 (≠ R197), G144 (≠ A226), A161 (≠ G243)
- binding zinc ion: H165 (= H247), C175 (= C257), C177 (= C259), C182 (= C264)
2yhyA Structure of n-acetylmannosamine kinase in complex with n- acetylmannosamine and adp (see paper)
27% identity, 68% coverage: 89:364/406 of query aligns to 6:279/308 of 2yhyA
- binding adenosine-5'-diphosphate: G11 (= G94), G12 (≠ R95), T13 (≠ Q96), N14 (≠ D97), R16 (≠ T99), T140 (≠ R222), G189 (≠ N271), L216 (≠ S298), V261 (≠ E343)
- binding calcium ion: N112 (≠ H194), N115 (≠ R197), G144 (≠ A226), A161 (≠ G243)
- binding zinc ion: H165 (= H247), C175 (= C257), C177 (= C259), C182 (= C264)
Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 18 papers)
27% identity, 68% coverage: 89:364/406 of query aligns to 410:688/722 of Q9Y223
- D413 (≠ R92) binding Mg(2+)
- G416 (≠ R95) binding an N-acyl-D-mannosamine 6-phosphate
- T417 (≠ Q96) binding ADP
- N418 (≠ D97) binding ADP
- R420 (≠ T99) binding ADP
- I472 (≠ V152) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 50% of the wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity
- G476 (= G156) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- R477 (≠ L157) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- T489 (≠ P169) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- N516 (≠ H194) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- D517 (= D195) active site; binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
- N519 (≠ R197) to S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910; mutation to S: Decreased N-acylmannosamine kinase activity.
- A524 (= A202) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
- F528 (= F206) to C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986; mutation to C: Decreased N-acylmannosamine kinase activity.
- G545 (= G223) binding an N-acyl-D-mannosamine 6-phosphate
- E566 (= E244) binding an N-acyl-D-mannosamine
- H569 (= H247) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; binding Zn(2+)
- V572 (= V250) to L: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70-80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
- G576 (= G254) to E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
- C579 (= C257) binding Zn(2+)
- C581 (= C259) binding Zn(2+)
- C586 (= C264) binding Zn(2+)
- I587 (≠ L265) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603; mutation to T: Decreased N-acylmannosamine kinase activity.
- E588 (= E266) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
- A630 (= A303) to T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
- A631 (= A304) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs121908626; to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 70% of wild-type activity; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs62541771; mutation A->V,T: Decreased N-acylmannosamine kinase activity.
Sites not aligning to the query:
- 13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
- 19 binding UDP
- 23 binding UDP
- 113 binding UDP
- 132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 10% of wild-type activity; impaired homohexamers formation
- 176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
- 177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to less than 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs539332585
- 200 I → F: in NM; uncertain significance; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 90% of wild-type activity; decreased N-acylmannosamine kinase activity; retains 75% of wild-type activity; dbSNP:rs369328625
- 206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
- 220 binding UDP
- 253 binding UDP
- 259 binding CMP-N-acetyl-beta-neuraminate
- 263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
- 266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
- 271 binding CMP-N-acetyl-beta-neuraminate
- 280 binding CMP-N-acetyl-beta-neuraminate
- 281 binding CMP-N-acetyl-beta-neuraminate
- 282 binding UDP
- 301 binding UDP
- 302 binding UDP
- 303 C → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; retains 80% of wild-type activity; decreased N-acylmannosamine kinase activity; corresponding to 60% of wild-type activity; requires 2 nucleotide substitutions; dbSNP:rs121908633
- 307 binding UDP
- 321 binding UDP
- 331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 20% of wild-type activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
- 378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; corresponding to 10-30% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs199877522
- 708 G → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; severely decreased; dbSNP:rs1554657922
- 712 M→T: Decreased N-acylmannosamine kinase activity.
3vovB Crystal structure of rok hexokinase from thermus thermophilus (see paper)
31% identity, 46% coverage: 149:335/406 of query aligns to 60:236/298 of 3vovB
2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
27% identity, 68% coverage: 89:364/406 of query aligns to 6:280/309 of 2yhwA
O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
26% identity, 63% coverage: 89:344/406 of query aligns to 410:671/722 of O35826