SitesBLAST
Comparing WP_038019545.1 NCBI__GCF_000423825.1:WP_038019545.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
1tuuA Acetate kinase crystallized with atpgs (see paper)
38% identity, 98% coverage: 1:373/381 of query aligns to 1:395/399 of 1tuuA
- active site: N7 (= N7), R91 (= R83), H180 (= H171), R241 (= R227), E384 (= E362)
- binding adenosine-5'-diphosphate: K14 (= K14), G210 (= G196), D283 (vs. gap), F284 (≠ D266), R285 (= R267), G331 (= G313), I332 (= I314), N335 (= N317)
- binding sulfate ion: R91 (= R83), H180 (= H171), G212 (= G198)
1tuuB Acetate kinase crystallized with atpgs (see paper)
38% identity, 98% coverage: 1:373/381 of query aligns to 1:395/398 of 1tuuB
- active site: N7 (= N7), R91 (= R83), H180 (= H171), R241 (= R227), E384 (= E362)
- binding adenosine monophosphate: D283 (vs. gap), R285 (= R267), G331 (= G313), I332 (= I314), N335 (= N317), S336 (≠ A318)
- binding trihydrogen thiodiphosphate: H180 (= H171), G212 (= G198), R241 (= R227)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
38% identity, 98% coverage: 1:373/381 of query aligns to 1:395/408 of P38502
- N7 (= N7) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S10) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (= S12) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K14) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R83) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V85) mutation to A: Decreases affinity for acetate.
- L122 (= L114) mutation to A: Decreases affinity for acetate.
- D148 (= D140) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F170) mutation to A: Decreases affinity for acetate.
- N211 (≠ S197) mutation to A: Slightly reduced enzyme activity.
- P232 (= P218) mutation to A: Decreases affinity for acetate.
- R241 (= R227) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E362) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
7fj9A Kpacka (pduw) with amppnp complex structure
41% identity, 98% coverage: 3:376/381 of query aligns to 4:394/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
41% identity, 98% coverage: 3:376/381 of query aligns to 4:394/395 of 7fj8A
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwsA
- active site: N8 (= N7), R83 (= R83), H172 (= H171), R233 (= R227)
- binding cytidine-5'-triphosphate: G202 (= G196), N203 (≠ S197), G204 (= G198), D275 (= D268), L276 (≠ M269), R277 (≠ A270), G323 (= G313), I324 (= I314), N327 (= N317)
- binding 1,2-ethanediol: V21 (≠ S20), C24 (≠ A23), H115 (= H115), N203 (≠ S197), T232 (= T226), R233 (= R227), K262 (≠ E256)
Sites not aligning to the query:
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwrA
- active site: N8 (= N7), R83 (= R83), H172 (= H171), R233 (= R227)
- binding cytidine-5'-monophosphate: G202 (= G196), N203 (≠ S197), D275 (= D268), L276 (≠ M269), R277 (≠ A270), G323 (= G313), I324 (= I314), N327 (= N317)
Sites not aligning to the query:
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwqA
- active site: N8 (= N7), R83 (= R83), H172 (= H171), R233 (= R227)
- binding guanosine-5'-triphosphate: H172 (= H171), N203 (≠ S197), G204 (= G198), D275 (= D268), L276 (≠ M269), R277 (≠ A270), E280 (≠ L273), G323 (= G313), I324 (= I314), N327 (= N317)
Sites not aligning to the query:
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwpA
- active site: N8 (= N7), R83 (= R83), H172 (= H171), R233 (= R227)
- binding 1,2-ethanediol: S11 (= S10), H115 (= H115), K262 (≠ E256)
- binding guanosine-5'-diphosphate: N203 (≠ S197), D275 (= D268), L276 (≠ M269), R277 (≠ A270), E280 (≠ L273), G323 (= G313), I324 (= I314), N327 (= N317), S328 (≠ A318)
Sites not aligning to the query:
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwoA
- active site: N8 (= N7), R83 (= R83), H172 (= H171), R233 (= R227)
- binding guanosine-5'-monophosphate: G202 (= G196), N203 (≠ S197), D275 (= D268), L276 (≠ M269), R277 (≠ A270), E280 (≠ L273), G323 (= G313), I324 (= I314), N327 (= N317)
- binding 1,2-ethanediol: E100 (≠ R100), N104 (≠ E104)
Sites not aligning to the query:
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwnA
- active site: N8 (= N7), R83 (= R83), H172 (= H171), R233 (= R227)
- binding adenosine-5'-tetraphosphate: H172 (= H171), H200 (= H194), N203 (≠ S197), G204 (= G198), D275 (= D268), L276 (≠ M269), R277 (≠ A270), G323 (= G313), I324 (= I314), N327 (= N317)
Sites not aligning to the query:
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwmA
- active site: N8 (= N7), R83 (= R83), H172 (= H171), R233 (= R227)
- binding adenosine-5'-triphosphate: H172 (= H171), H200 (= H194), N203 (≠ S197), G204 (= G198), D275 (= D268), L276 (≠ M269), R277 (≠ A270), G323 (= G313), I324 (= I314), N327 (= N317)
- binding 1,2-ethanediol: H172 (= H171), R233 (= R227)
Sites not aligning to the query:
4fwkA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with amp (see paper)
41% identity, 89% coverage: 3:341/381 of query aligns to 4:351/394 of 4fwkA