SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_038070547.1 NCBI__GCF_000526715.1:WP_038070547.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
41% identity, 76% coverage: 22:133/148 of query aligns to 5:116/204 of 7bgnA

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7bgnA

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binding adenosine monophosphate: W60 (= W77), K62 (= K79), G63 (= G80), T65 (≠ E82), S66 (= S83), T94 (≠ A111), H96 (= H113)
binding zinc ion: D78 (= D95), D80 (= D97), D82 (= D99), C95 (= C112), C102 (= C119)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
43% identity, 70% coverage: 22:124/148 of query aligns to 7:109/213 of 7bgmA

query
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7bgmA

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binding zinc ion: D80 (= D95), D82 (= D97), D84 (= D99), C97 (= C112), C104 (= C119)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lA Crystal structure of bi-functional enzyme (see paper)
45% identity, 60% coverage: 32:120/148 of query aligns to 16:104/200 of 6j2lA

query
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6j2lA

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binding zinc ion: D79 (= D95), D81 (= D97), D83 (= D99), C96 (= C112), C103 (= C119)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
42% identity, 60% coverage: 32:120/148 of query aligns to 15:97/185 of 6j2lB

query
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6j2lB

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binding zinc ion: D78 (= D95), D80 (= D97), D82 (= D99)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>WP_038070547.1 NCBI__GCF_000526715.1:WP_038070547.1
MTTSFKSLEKAHQGDTFDWAELKSQLKFDDKGLIPAIAQQHDTGEVLMMAWMNADSIAET
LKTGRVCYWSRSRQSYWRKGEESGQIQVLKSMRLDCDGDTILLLVDQTGPACHTGRKSCF
YTEISPESAVILTDPLIDPETLYAKKEK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory