SitesBLAST
Comparing WP_038070594.1 NCBI__GCF_000526715.1:WP_038070594.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
23% identity, 29% coverage: 165:541/1283 of query aligns to 50:366/467 of 6lpnB
- binding flavin-adenine dinucleotide: P76 (= P191), G78 (= G193), G79 (= G194), N80 (≠ G195), T81 (= T196), G82 (= G197), M83 (≠ Y198), G86 (= G201), S87 (≠ A202), L140 (≠ P263), A142 (≠ S265), C146 (≠ S269), H147 (≠ C270), G150 (= G273), N151 (= N274), A153 (= A276), T154 (≠ M277), G208 (= G377), I212 (≠ L381), I213 (= I382)
Sites not aligning to the query:
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
23% identity, 29% coverage: 165:541/1283 of query aligns to 49:365/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R508), T337 (= T511), K348 (= K522)
- binding flavin-adenine dinucleotide: P75 (= P191), Q76 (≠ R192), G77 (= G193), G78 (= G194), N79 (≠ G195), T80 (= T196), G81 (= G197), M82 (≠ Y198), G85 (= G201), S86 (≠ A202), L139 (≠ P263), G140 (≠ T264), A141 (≠ S265), C145 (≠ S269), G149 (= G273), N150 (= N274), A152 (= A276), T153 (≠ M277), G157 (= G281), G207 (= G377), I212 (= I382)
Sites not aligning to the query:
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
23% identity, 29% coverage: 165:541/1283 of query aligns to 49:365/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (= P191), G77 (= G193), G78 (= G194), N79 (≠ G195), T80 (= T196), G81 (= G197), G85 (= G201), S86 (≠ A202), L139 (≠ P263), G140 (≠ T264), A141 (≠ S265), C145 (≠ S269), H146 (≠ C270), G148 (= G272), G149 (= G273), N150 (= N274), A152 (= A276), T153 (≠ M277), A155 (= A279), E206 (= E376), G207 (= G377), I211 (≠ L381), I212 (= I382)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R508), T337 (= T511), K348 (= K522)
Sites not aligning to the query:
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
23% identity, 29% coverage: 165:541/1283 of query aligns to 49:365/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (= P191), G77 (= G193), G78 (= G194), N79 (≠ G195), T80 (= T196), G81 (= G197), G85 (= G201), S86 (≠ A202), L139 (≠ P263), G140 (≠ T264), A141 (≠ S265), C145 (≠ S269), H146 (≠ C270), G149 (= G273), N150 (= N274), A152 (= A276), T153 (≠ M277), A155 (= A279), G157 (= G281), E206 (= E376), G207 (= G377), I211 (≠ L381), I212 (= I382)
- binding d-malate: M82 (≠ Y198), R333 (= R508), T337 (= T511), K348 (= K522)
Sites not aligning to the query:
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
23% identity, 29% coverage: 165:541/1283 of query aligns to 49:365/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R508), T337 (= T511), K348 (= K522)
- binding flavin-adenine dinucleotide: P75 (= P191), G77 (= G193), G78 (= G194), N79 (≠ G195), T80 (= T196), G81 (= G197), M82 (≠ Y198), G85 (= G201), S86 (≠ A202), L139 (≠ P263), G140 (≠ T264), A141 (≠ S265), C145 (≠ S269), G149 (= G273), N150 (= N274), A152 (= A276), T153 (≠ M277), A155 (= A279), G157 (= G281), G207 (= G377), I212 (= I382)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
23% identity, 29% coverage: 165:541/1283 of query aligns to 102:418/521 of Q8N465
- S109 (≠ E172) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ I190) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G194) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V211) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L217) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V243) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (≠ A260) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A276) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P302) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (≠ A304) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S497) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R508) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (= T511) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ A520) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K522) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
- 419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- 426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- 434 binding Zn(2+); H→A: Loss of catalytic activity.
- 436 G → V: slight reduction in catalytic activity
- 439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- 441 binding Zn(2+); H→A: Loss of catalytic activity.
- 443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
- 444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- 446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- 475 binding Zn(2+); E→A: Loss of catalytic activity.
- 476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
- 477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
25% identity, 31% coverage: 148:540/1283 of query aligns to 25:351/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ Y198), R317 (= R508), W321 (vs. gap)
- binding flavin-adenine dinucleotide: P68 (= P191), G70 (= G193), T71 (≠ G194), G72 (= G195), T73 (= T196), G74 (= G197), G78 (= G201), V79 (≠ A202), L90 (≠ T214), P132 (= P263), G133 (≠ T264), A134 (≠ S265), G140 (= G273), M141 (≠ N274), A143 (= A276), T144 (≠ M277), A146 (= A279), S147 (≠ G280), E200 (= E376), G201 (= G377), I206 (= I382), W321 (vs. gap), Y322 (vs. gap)
Sites not aligning to the query:
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
24% identity, 31% coverage: 148:542/1283 of query aligns to 25:354/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R508), W322 (vs. gap)
- binding flavin-adenine dinucleotide: P68 (= P191), G70 (= G193), T71 (≠ G194), G72 (= G195), T73 (= T196), G74 (= G197), G78 (= G201), V79 (≠ A202), L90 (≠ T214), P132 (= P263), G133 (≠ T264), A134 (≠ S265), G140 (= G273), M141 (≠ N274), A143 (= A276), T144 (≠ M277), A146 (= A279), S147 (≠ G280), E200 (= E376), G201 (= G377), I206 (= I382), W322 (vs. gap)
Sites not aligning to the query:
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
24% identity, 31% coverage: 148:540/1283 of query aligns to 25:353/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ A155), P68 (= P191), G70 (= G193), T71 (≠ G194), G72 (= G195), T73 (= T196), G74 (= G197), G78 (= G201), V79 (≠ A202), L90 (≠ T214), P132 (= P263), G133 (≠ T264), A134 (≠ S265), G140 (= G273), M141 (≠ N274), A143 (= A276), T144 (≠ M277), A146 (= A279), S147 (≠ G280), E200 (= E376), G201 (= G377), I206 (= I382), W323 (vs. gap)
- binding pyruvic acid: R319 (= R508)
Sites not aligning to the query:
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
24% identity, 31% coverage: 148:542/1283 of query aligns to 25:355/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P191), G70 (= G193), T71 (≠ G194), G72 (= G195), T73 (= T196), G74 (= G197), G78 (= G201), V79 (≠ A202), L90 (≠ T214), P132 (= P263), G133 (≠ T264), A134 (≠ S265), G140 (= G273), M141 (≠ N274), A143 (= A276), T144 (≠ M277), A146 (= A279), S147 (≠ G280), E200 (= E376), G201 (= G377), I206 (= I382), Y324 (vs. gap)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R508), W323 (vs. gap)
Sites not aligning to the query:
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
24% identity, 31% coverage: 148:542/1283 of query aligns to 25:355/456 of 8jdqA