SitesBLAST
Comparing WP_038211943.1 NCBI__GCF_000745855.1:WP_038211943.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
54% identity, 99% coverage: 3:262/262 of query aligns to 2:257/257 of 6pejA
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
37% identity, 98% coverage: 5:261/262 of query aligns to 3:247/247 of 4jroC
- active site: G16 (= G18), S142 (= S145), Q152 (≠ V155), Y155 (= Y158), K159 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (≠ G16), R15 (≠ G17), G16 (= G18), I17 (= I19), N35 (vs. gap), Y36 (≠ V37), N37 (≠ D38), G38 (≠ R39), S39 (≠ A40), N63 (≠ D64), V64 (= V65), N90 (= N91), A91 (= A92), I93 (≠ V94), I113 (≠ V114), S142 (= S145), Y155 (= Y158), K159 (= K162), P185 (= P188), I188 (≠ V191), T190 (= T193)
3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
35% identity, 96% coverage: 11:262/262 of query aligns to 5:257/257 of 3a28C
- active site: G12 (= G18), S140 (≠ A144), Y153 (= Y158), K157 (= K162), L198 (≠ F203)
- binding nicotinamide-adenine-dinucleotide: G8 (= G14), Q11 (≠ G17), I13 (= I19), D32 (= D38), L33 (vs. gap), Q36 (≠ A40), L59 (≠ A63), D60 (= D64), V61 (= V65), N87 (= N91), S140 (≠ A144), Y153 (= Y158), K157 (= K162), P183 (= P188), V186 (= V191), T188 (= T193), M190 (= M195), W191 (= W196)
Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
35% identity, 96% coverage: 11:262/262 of query aligns to 6:258/258 of Q9ZNN8
- QGI 12:14 (≠ GGI 17:19) binding NAD(+)
- D33 (= D38) binding NAD(+)
- Q37 (≠ A40) binding NAD(+)
- DV 61:62 (= DV 64:65) binding NAD(+)
- N88 (= N91) binding NAD(+)
- I142 (≠ S145) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- F148 (≠ E152) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- Y154 (= Y158) binding NAD(+)
- K158 (= K162) binding NAD(+)
- PGIVGT 184:189 (≠ PGVVDT 188:193) binding NAD(+)
3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
38% identity, 98% coverage: 7:262/262 of query aligns to 1:255/255 of 3wyeA
- active site: G12 (= G18), S138 (= S145), Y151 (= Y158), K155 (= K162), L196 (≠ F203)
- binding nicotinamide-adenine-dinucleotide: G8 (= G14), Q11 (≠ G17), G12 (= G18), I13 (= I19), D32 (= D38), Y33 (≠ R39), V57 (vs. gap), D58 (= D64), V59 (= V65), N85 (= N91), A86 (= A92), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (= P188), G182 (= G189), V184 (= V191), T186 (= T193), M188 (= M195), W189 (= W196)
C1DMX5 L-rhamnose 1-dehydrogenase (NAD(P)(+)); RhaDH; AvLRA1; EC 1.1.1.378 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
37% identity, 98% coverage: 5:262/262 of query aligns to 3:254/256 of C1DMX5
- G12 (= G14) binding NADP(+)
- S14 (≠ G16) binding NADP(+)
- R15 (≠ G17) binding NADP(+); mutation to T: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+).
- I17 (= I19) binding NADP(+)
- S37 (≠ R39) binding NADP(+); mutation to H: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+) and an increase in catalytic efficiency with NAD(+).
- D66 (≠ R67) binding NADP(+)
- A67 (≠ R68) binding NADP(+)
- N93 (= N91) binding NADP(+)
- F99 (≠ L97) mutation F->A,Y: Shows a strong decrease in catalytic efficiency with L-rhamnose, L-lyxose and L-mannose.
- S146 (= S145) binding beta-L-rhamnose
- S148 (≠ A147) binding beta-L-rhamnose
- Q156 (≠ V155) binding beta-L-rhamnose; mutation to A: Almost loss of activity with L-rhamnose as substrate.
- Y159 (= Y158) binding beta-L-rhamnose; binding NADP(+)
- K163 (= K162) binding NADP(+)
- T191 (≠ V190) binding beta-L-rhamnose; mutation to F: Retains 4% of wild-type activity with L-rhamnose as substrate.
- I192 (≠ V191) binding NADP(+)
- I196 (≠ M195) mutation to A: Shows a strong decrease in catalytic efficiency with L-rhamnose as substrate, but does not affect catalytic efficiency with L-lyxose and L-mannose.
- N197 (≠ R205) binding beta-L-rhamnose
- D200 (≠ N208) mutation to A: Retains 16% of wild-type activity with L-rhamnose as substrate.; mutation to H: Retains 22% of wild-type activity with L-rhamnose as substrate.
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
37% identity, 98% coverage: 5:262/262 of query aligns to 5:258/258 of 3ak4A
- active site: G18 (= G18), S141 (= S145), L151 (≠ V155), Y154 (= Y158), K158 (= K162), E199 (≠ F203)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ G17), G18 (= G18), I19 (= I19), D38 (= D38), L39 (≠ R39), V60 (≠ A63), D61 (= D64), V62 (= V65), N88 (= N91), A89 (= A92), G90 (≠ A93), T139 (≠ M143), S141 (= S145), Y154 (= Y158), K158 (= K162), G185 (= G189), V187 (= V191), T189 (= T193), M191 (= M195)
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
37% identity, 98% coverage: 5:262/262 of query aligns to 3:254/256 of 7do7A
- active site: G16 (= G18), S146 (= S145), Y159 (= Y158)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), R15 (≠ G17), G16 (= G18), I17 (= I19), S37 (≠ R39), D66 (≠ R67), A67 (≠ R68), N93 (= N91), A94 (= A92), G95 (≠ A93), I96 (≠ V94), V144 (≠ M143), S145 (≠ A144), S146 (= S145), Y159 (= Y158), K163 (= K162), P189 (= P188), G190 (= G189), I192 (≠ V191), T194 (= T193), I196 (≠ M195)
- binding beta-L-rhamnopyranose: F99 (≠ L97), S146 (= S145), S148 (≠ A147), Q156 (≠ V155), Y159 (= Y158), N197 (≠ R205), D235 (= D243), M236 (≠ E244), R238 (= R246)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
37% identity, 98% coverage: 5:262/262 of query aligns to 3:254/256 of 7b81A
- active site: G16 (= G18), S146 (= S145), Y159 (= Y158)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), S14 (≠ G16), R15 (≠ G17), I17 (= I19), D66 (≠ R67), A67 (≠ R68), N93 (= N91), A94 (= A92), G95 (≠ A93), I96 (≠ V94), T116 (≠ V114), V144 (≠ M143), S146 (= S145), Y159 (= Y158), K163 (= K162), P189 (= P188), G190 (= G189), I192 (≠ V191), T194 (= T193), I196 (≠ M195)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
36% identity, 98% coverage: 4:261/262 of query aligns to 4:244/244 of 4nbuB
- active site: G18 (= G18), N111 (= N115), S139 (= S145), Q149 (≠ V155), Y152 (= Y158), K156 (= K162)
- binding acetoacetyl-coenzyme a: D93 (≠ L97), K98 (≠ E102), S139 (= S145), N146 (≠ E152), V147 (≠ A153), Q149 (≠ V155), Y152 (= Y158), F184 (≠ V190), M189 (= M195), K200 (≠ G212)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), N17 (≠ G17), G18 (= G18), I19 (= I19), D38 (= D38), F39 (≠ R39), V59 (≠ A63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (≠ A93), I90 (≠ V94), T137 (≠ M143), S139 (= S145), Y152 (= Y158), K156 (= K162), P182 (= P188), F184 (≠ V190), T185 (≠ V191), T187 (= T193), M189 (= M195)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
34% identity, 98% coverage: 1:258/262 of query aligns to 1:251/255 of 5itvA
- active site: G18 (= G18), S141 (= S145), Y154 (= Y158), K158 (= K162)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (≠ G17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (≠ A40), T61 (≠ A63), I63 (≠ V65), N89 (= N91), G91 (≠ A93), T139 (≠ M143), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (= P188), G185 (= G189), I186 (≠ V190), I187 (≠ V191)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
33% identity, 98% coverage: 7:262/262 of query aligns to 2:256/256 of Q48436
- 6:33 (vs. 11:38, 46% identical) binding NAD(+)
- D59 (= D64) binding NAD(+)
- K156 (= K162) binding NAD(+)
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
35% identity, 98% coverage: 7:262/262 of query aligns to 2:256/256 of 1gegE
- active site: G13 (= G18), S139 (= S145), Y152 (= Y158), K156 (= K162), V197 (≠ F203)
- binding alpha-D-glucopyranose: R63 (= R68), D64 (≠ A69), F67 (≠ D72), E123 (≠ R128)
- binding nicotinamide-adenine-dinucleotide: G9 (= G14), Q12 (≠ G17), I14 (= I19), D33 (= D38), Y34 (≠ R39), V58 (vs. gap), D59 (= D64), V60 (= V65), N86 (= N91), A87 (= A92), I109 (≠ V114), S139 (= S145), Y152 (= Y158), K156 (= K162), P182 (= P188), V185 (= V191), T187 (= T193), M189 (= M195)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
35% identity, 98% coverage: 4:261/262 of query aligns to 5:247/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G14), R18 (≠ G17), G19 (= G18), I20 (= I19), D39 (= D38), R40 (= R39), C63 (≠ A63), I65 (≠ V65), N91 (= N91), G93 (≠ A93), I94 (≠ V94), V114 (= V114), Y155 (= Y158), K159 (= K162), I188 (≠ V191), T190 (= T193), T193 (≠ P210)
7do6A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NADP bound-form) (see paper)
35% identity, 98% coverage: 5:262/262 of query aligns to 3:245/247 of 7do6A
- active site: G16 (= G18), S146 (= S145), Y159 (= Y158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (≠ G16), R15 (≠ G17), G16 (= G18), I17 (= I19), H36 (≠ D38), S37 (≠ R39), G42 (≠ P44), D66 (≠ R67), A67 (≠ R68), N93 (= N91), A94 (= A92), G95 (≠ A93), I96 (≠ V94), T116 (≠ V114), S146 (= S145), Y159 (= Y158), K163 (= K162), I192 (≠ V191)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
37% identity, 98% coverage: 2:258/262 of query aligns to 8:262/267 of Q9LBG2
- 17:42 (vs. 11:36, 54% identical) binding NAD(+)
- E103 (≠ F95) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
5t5qC Crystal structure of short-chain dehydrogenase/reductase sdr:glucose/ribitol dehydrogenase from brucella melitensis
32% identity, 100% coverage: 1:261/262 of query aligns to 1:244/245 of 5t5qC
- active site: G18 (= G18), S140 (= S145), N150 (≠ V155), Y153 (= Y158), K157 (= K162)
- binding nicotinamide-adenine-dinucleotide: N16 (≠ G16), G17 (= G17), G18 (= G18), I19 (= I19), D38 (= D38), L39 (≠ R39), D63 (= D64), A64 (≠ V65), S90 (≠ N91), I113 (≠ V114), Y153 (= Y158), K157 (= K162), P182 (= P188), I185 (≠ V191), T187 (= T193), M189 (= M195)
1iy8A Crystal structure of levodione reductase (see paper)
37% identity, 97% coverage: 4:258/262 of query aligns to 1:253/258 of 1iy8A
- active site: G15 (= G18), S143 (= S145), Q153 (≠ V155), Y156 (= Y158), K160 (= K162)
- binding nicotinamide-adenine-dinucleotide: G11 (= G14), S14 (≠ G17), G15 (= G18), L16 (≠ I19), D35 (= D38), V36 (≠ R39), A62 (= A63), D63 (= D64), V64 (= V65), N90 (= N91), G92 (≠ A93), I93 (≠ V94), T141 (≠ M143), S143 (= S145), Y156 (= Y158), K160 (= K162), P186 (= P188), G187 (= G189), T191 (= T193), P192 (= P194), M193 (= M195)
4za2D Crystal structure of pectobacterium carotovorum 2-keto-3-deoxy-d- gluconate dehydrogenase complexed with NAD+ (see paper)
33% identity, 97% coverage: 5:258/262 of query aligns to 8:243/247 of 4za2D
- binding nicotinamide-adenine-dinucleotide: G17 (= G14), D19 (≠ G16), L22 (≠ I19), I42 (≠ P41), D65 (= D64), M66 (≠ V65), N92 (= N91), A93 (= A92), G94 (≠ A93), L115 (≠ V114), I143 (≠ M143), S145 (= S145), Y158 (= Y158), K162 (= K162), G189 (= G189), M191 (≠ V191), T193 (= T193), N195 (≠ M195)
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
36% identity, 97% coverage: 4:258/262 of query aligns to 3:245/249 of 4bmsF
- active site: S137 (= S145), H147 (≠ V155), Y150 (= Y158), K154 (= K162), Q195 (≠ R205)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G14), N15 (≠ G16), S16 (≠ G17), I18 (= I19), R38 (= R39), R39 (≠ A40), A59 (= A63), D60 (= D64), V61 (= V65), N87 (= N91), S88 (≠ A92), G89 (≠ A93), V110 (= V114), S137 (= S145), Y150 (= Y158), K154 (= K162), G181 (= G189), I183 (≠ V191), T185 (= T193), I187 (≠ M195)
Query Sequence
>WP_038211943.1 NCBI__GCF_000745855.1:WP_038211943.1
MTPRLDQRHVLITGAGGGIGLAMALACRAEGARCTLVDRAPQAPAEAAAALASHPQALHY
VAADVTRRADIDRLLPEAVARFGPVHTLINNAAVFDLAPLLESDEASFDRLFAVNVKGMF
FVMQAVLRHMVEAGTQGASVINMASQAGRRGEALVSHYCATKAAVISYTQSAALAMAPHG
IRVNGIAPGVVDTPMWEQVDGLFARHENLPRGEKKRQVGLAVPLGRMGLPQDVAGAAVFL
ASDEARYITAQTLNVDGGNVMN
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory