SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_038211951.1 NCBI__GCF_000745855.1:WP_038211951.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7cagA Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
27% identity, 98% coverage: 2:281/285 of query aligns to 6:280/285 of 7cagA

query
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7cagA

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binding alpha-D-glucopyranose: E159 (≠ A159), K162 (≠ Q161), R216 (≠ E215), D219 (≠ F218)

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
30% identity, 68% coverage: 79:273/285 of query aligns to 280:487/490 of 4ki0F

query
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4ki0F

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binding alpha-D-glucopyranose: Y310 (≠ F108), N361 (≠ W160), G365 (vs. gap), Y368 (≠ F165), S418 (≠ E215), N422 (≠ L219)

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
30% identity, 68% coverage: 79:273/285 of query aligns to 295:502/514 of P02916

query
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P02916

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A

L334 (= L117) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ I156) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ W160) mutation N->K,H: No growth on maltose.
G380 (vs. gap) mutation G->C,S: No growth on maltose.
E401 (= E183) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A185) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G189) mutation G->A,P: No effect.
P420 (= P202) mutation to A: No effect.

P94529 Arabinooligosaccharides transport system permease protein AraP from Bacillus subtilis (strain 168) (see paper)
23% identity, 91% coverage: 2:260/285 of query aligns to 28:286/313 of P94529

query
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P94529

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A

E205 (= E180) mutation to A: Has no effect on the growth rate.
E208 (= E183) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D188) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

Query Sequence

>WP_038211951.1 NCBI__GCF_000745855.1:WP_038211951.1
MNRLLPRALMAPAVLTLFLWMIVPLAMTLYFSFVNYNLMQPGEHPFAGIDNFHYFVTDPD
FWPSVGNTLLLIGSVIAATVVLGIGLALLIDAPFPGRGIVRVLLISPFFVMPTVNALLWK
HMMMNPIYGILADVWRFFGAEPVDWMADWPLLSVIIMVAWQWLPFACLIFMTSLQSLDRE
QMEAARMDGAGPFQRFFYLVLPHLARPMAVVIMIEMIFLLSVFAEIAITTAGGPGNASTN
LTYLIYKQSLMNFDVGIASAGALFAVILANVVAAFLIRIIGKNLD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory