SitesBLAST
Comparing WP_038858356.1 NCBI__GCF_001277175.1:WP_038858356.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P27830 dTDP-glucose 4,6-dehydratase 2; EC 4.2.1.46 from Escherichia coli (strain K12) (see 2 papers)
83% identity, 99% coverage: 1:353/355 of query aligns to 1:353/355 of P27830
- FI 12:13 (= FI 12:13) binding NAD(+)
- DKLT 33:36 (= DKLT 33:36) binding NAD(+)
- DI 59:60 (= DI 59:60) binding NAD(+)
- T100 (= T100) binding NAD(+)
- D135 (= D135) active site, Proton donor
- E136 (= E136) active site, Proton acceptor
- Y160 (= Y160) active site, Proton acceptor
- YSASK 160:164 (= YSASK 160:164) binding NAD(+)
- N190 (= N190) binding NAD(+)
1bxkB Dtdp-glucose 4,6-dehydratase from e. Coli
81% identity, 99% coverage: 1:350/355 of query aligns to 1:344/344 of 1bxkB
- active site: S125 (≠ A125), T134 (= T134), D135 (= D135), E136 (= E136), S158 (= S158), Y160 (= Y160), S161 (= S161), K164 (= K164)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), I13 (= I13), D33 (= D33), K34 (= K34), L35 (= L35), T36 (= T36), A38 (= A38), G39 (= G39), D59 (= D59), I60 (= I60), L81 (= L81), A83 (= A83), T100 (= T100), I132 (= I132), S133 (= S133), T134 (= T134), K164 (= K164), C187 (= C187)
1kewA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with thymidine diphosphate bound (see paper)
71% identity, 97% coverage: 3:347/355 of query aligns to 2:350/361 of 1kewA
- active site: T133 (= T134), D134 (= D135), E135 (= E136), L152 (vs. gap), L154 (≠ F147), F155 (= F148), T158 (= T151), Y167 (= Y160), K171 (= K164)
- binding nicotinamide-adenine-dinucleotide: G10 (= G11), F11 (= F12), I12 (= I13), D32 (= D33), K33 (= K34), L34 (= L35), T35 (= T36), A37 (= A38), G38 (= G39), D58 (= D59), I59 (= I60), L80 (= L81), A81 (= A82), A82 (= A83), S84 (= S85), T99 (= T100), I131 (= I132), S132 (= S133), T133 (= T134), Y167 (= Y160), K171 (= K164), C194 (= C187), N196 (= N189), N197 (= N190)
- binding thymidine-5'-diphosphate: E135 (= E136), N196 (= N189), K206 (= K199), L207 (= L200), P222 (= P215), Y224 (= Y217), R231 (= R224), N266 (= N259), R297 (= R294), H300 (= H297)
Sites not aligning to the query:
1keuA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with dtdp-d-glucose bound (see paper)
71% identity, 97% coverage: 3:347/355 of query aligns to 2:350/361 of 1keuA
- active site: T133 (= T134), D134 (= D135), E135 (= E136), L152 (vs. gap), L154 (≠ F147), F155 (= F148), T158 (= T151), Y167 (= Y160), K171 (= K164)
- binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S84 (= S85), T133 (= T134), D134 (= D135), E135 (= E136), Y167 (= Y160), N196 (= N189), K206 (= K199), L207 (= L200), P222 (= P215), Y224 (= Y217), R231 (= R224), N266 (= N259), R297 (= R294), H300 (= H297)
- binding nicotinamide-adenine-dinucleotide: G10 (= G11), F11 (= F12), I12 (= I13), D32 (= D33), K33 (= K34), L34 (= L35), T35 (= T36), G38 (= G39), D58 (= D59), L80 (= L81), A81 (= A82), A82 (= A83), S84 (= S85), T99 (= T100), S132 (= S133), T133 (= T134), Y167 (= Y160), K171 (= K164), C194 (= C187), N196 (= N189), N197 (= N190)
Sites not aligning to the query:
P26391 dTDP-glucose 4,6-dehydratase; EC 4.2.1.46 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
71% identity, 97% coverage: 3:347/355 of query aligns to 2:350/361 of P26391
Sites not aligning to the query:
8du1A Crystal structure of NAD bound dtdp-glucose 4,6-dehydratase from elizabethkingia anophelis
54% identity, 98% coverage: 1:347/355 of query aligns to 3:353/361 of 8du1A
- binding nicotinamide-adenine-dinucleotide: G10 (= G8), G13 (= G11), F14 (= F12), I15 (= I13), D36 (= D33), A37 (≠ K34), L38 (= L35), T39 (= T36), G42 (= G39), D62 (= D59), I63 (= I60), L84 (= L81), A85 (= A82), A86 (= A83), T103 (= T100), S143 (= S133), T144 (= T134), Y169 (= Y160), K173 (= K164), C196 (= C187)
2hunA Crystal structure of hypothetical protein ph0414 from pyrococcus horikoshii ot3
49% identity, 96% coverage: 3:343/355 of query aligns to 3:318/329 of 2hunA
- active site: T125 (= T134), D126 (= D135), E127 (= E136), Y149 (= Y160), K153 (= K164)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), I13 (= I13), D34 (= D33), K35 (= K34), S40 (≠ G39), D60 (= D59), V61 (≠ I60), L80 (= L81), A81 (= A82), A82 (= A83), S99 (≠ T100), T125 (= T134), K153 (= K164), C176 (= C187), T177 (≠ S188), N178 (= N189), N179 (= N190)
1kerB The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with dtdp-d-glucose bound (see paper)
48% identity, 95% coverage: 1:339/355 of query aligns to 3:329/347 of 1kerB
- active site: T124 (= T134), D125 (= D135), E126 (= E136), Y160 (= Y160), K164 (= K164)
- binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S85 (= S85), N87 (≠ V87), T124 (= T134), D125 (= D135), E126 (= E136), Y160 (= Y160), N189 (= N189), K199 (= K199), F200 (≠ L200), R203 (≠ L203), Q204 (≠ T204), K215 (≠ P215), L216 (≠ V216), Y217 (= Y217), R224 (= R224), N259 (= N259), R283 (= R294), H286 (= H297)
- binding nicotinamide-adenine-dinucleotide: G13 (= G11), F14 (= F12), I15 (= I13), D36 (= D33), K37 (= K34), L38 (= L35), T39 (= T36), G42 (= G39), D61 (= D59), I62 (= I60), Y81 (≠ L81), A82 (= A82), A83 (= A83), S85 (= S85), T100 (= T100), S123 (= S133), T124 (= T134), Y160 (= Y160), K164 (= K164), C187 (= C187), N190 (= N190)
1ketA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with thymidine diphosphate bound (see paper)
48% identity, 95% coverage: 1:339/355 of query aligns to 2:328/346 of 1ketA
- active site: T123 (= T134), D124 (= D135), E125 (= E136), Y159 (= Y160), K163 (= K164)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), F13 (= F12), I14 (= I13), D35 (= D33), K36 (= K34), L37 (= L35), T38 (= T36), A40 (= A38), G41 (= G39), D60 (= D59), I61 (= I60), Y80 (≠ L81), A82 (= A83), S84 (= S85), T99 (= T100), S122 (= S133), T123 (= T134), Y159 (= Y160), K163 (= K164)
- binding thymidine-5'-diphosphate: E125 (= E136), N188 (= N189), F199 (≠ L200), R202 (≠ L203), Q203 (≠ T204), K214 (≠ P215), Y216 (= Y217), R223 (= R224), N258 (= N259), R282 (= R294), H285 (= H297)
1kepA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with dtdp-xylose bound (see paper)
48% identity, 95% coverage: 1:339/355 of query aligns to 2:328/346 of 1kepA
- active site: T123 (= T134), D124 (= D135), E125 (= E136), Y159 (= Y160), K163 (= K164)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), F13 (= F12), I14 (= I13), D35 (= D33), K36 (= K34), L37 (= L35), T38 (= T36), G41 (= G39), D60 (= D59), I61 (= I60), Y80 (≠ L81), A81 (= A82), A82 (= A83), S84 (= S85), T99 (= T100), S122 (= S133), Y159 (= Y160), K163 (= K164), N189 (= N190)
- binding thymidine-5'-diphospho-beta-d-xylose: S84 (= S85), T123 (= T134), E125 (= E136), Y159 (= Y160), N188 (= N189), K198 (= K199), R223 (= R224), R282 (= R294)
P95780 dTDP-glucose 4,6-dehydratase; EC 4.2.1.46 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see paper)
47% identity, 95% coverage: 2:339/355 of query aligns to 5:330/348 of P95780
- FI 15:16 (= FI 12:13) binding NAD(+)
- DKLT 37:40 (= DKLT 33:36) binding NAD(+)
- DI 62:63 (= DI 59:60) binding NAD(+)
- YAAES 82:86 (≠ LAAES 81:85) binding NAD(+)
- N88 (≠ V87) binding substrate
- T101 (= T100) binding NAD(+)
- T125 (= T134) binding substrate
- N190 (= N189) binding substrate
- N191 (= N190) binding NAD(+)
- KFIPRQ 200:205 (≠ KLIPLT 199:204) binding substrate
- KLY 216:218 (≠ PVY 215:217) binding substrate
- R225 (= R224) binding substrate
- N260 (= N259) binding substrate
1r66A Crystal structure of desiv (dtdp-glucose 4,6-dehydratase) from streptomyces venezuelae with NAD and tyd bound (see paper)
48% identity, 96% coverage: 3:342/355 of query aligns to 2:322/322 of 1r66A