SitesBLAST

5bjvA X-ray structure of the pglf udp-n-acetylglucosamine 4,6-dehydratase from campylobacterjejuni, d396n/k397a variant in complex with udp-n- acrtylglucosamine (see paper)
48% identity, 56% coverage: 261:603/618 of query aligns to 1:335/339 of 5bjvA

query
sites
5bjvA

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binding nicotinamide-adenine-dinucleotide: G31 (= G291), G34 (= G294), T35 (≠ S295), I36 (= I296), D56 (= D316), H57 (≠ I317), S82 (= S344), I83 (= I345), A104 (= A366), A105 (= A367), A106 (= A368), K108 (= K370), N123 (= N385), I146 (= I408), K162 (= K424), F184 (= F447), G185 (= G448), N186 (= N449), V187 (= V450), S190 (= S453), S191 (≠ N454)
binding uridine-diphosphate-n-acetylglucosamine: K108 (= K370), H109 (= H371), T148 (= T410), G185 (= G448), N186 (= N449), S193 (= S456), V194 (= V457), T209 (= T472), L210 (≠ V473), T211 (= T474), I215 (= I478), R217 (= R480), R276 (= R542), E279 (= E545)

6bwcC X-ray structure of pen from bacillus thuringiensis (see paper)
41% identity, 47% coverage: 281:568/618 of query aligns to 2:288/327 of 6bwcC

query
sites
6bwcC

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G291), T14 (≠ G293), G15 (= G294), T16 (≠ S295), I17 (= I296), S37 (≠ D316), R38 (≠ I317), S39 (≠ Y318), D63 (≠ S344), I64 (= I345), V83 (≠ A366), A84 (= A367), K87 (= K370), T125 (≠ I408), S127 (≠ T410), Y137 (≠ M420), K141 (= K424), F167 (= F447), V170 (= V450), S173 (= S453), R174 (≠ N454)
binding uridine-diphosphate-n-acetylglucosamine: K87 (= K370), H88 (= H371), S127 (≠ T410), N128 (≠ D411), Y137 (≠ M420), N169 (= N449), S176 (= S456), V177 (= V457), L180 (= L460), T192 (= T472), T194 (= T474), M198 (≠ I478), R200 (= R480), L234 (≠ I515), E265 (= E545)

2gn8A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp (see paper)
35% identity, 47% coverage: 282:571/618 of query aligns to 2:281/327 of 2gn8A

query
sites
2gn8A

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G291), T13 (≠ G293), G14 (= G294), S15 (= S295), F16 (≠ I296), S37 (≠ Y318), R38 (≠ E319), D39 (≠ N320), K42 (≠ Y323), D61 (≠ S344), V62 (≠ I345), A81 (= A366), A82 (= A367), A83 (= A368), K85 (= K370), T100 (≠ N385), L123 (≠ I408), S124 (= S409), K139 (= K424), Y165 (≠ F447), G166 (= G448), V168 (= V450), S171 (= S453), R172 (≠ N454)
binding uridine-5'-diphosphate: K127 (= K412), N167 (= N449), V175 (= V457), P191 (≠ T472), I192 (≠ V473), T193 (= T474), M197 (≠ I478), R199 (= R480), M233 (≠ I515), R252 (= R542)

O25511 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
35% identity, 48% coverage: 277:571/618 of query aligns to 3:287/333 of O25511

query
sites
O25511

N

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S

P

E

V

I

T

P

V

I

T

T

D

D

P

I

K

R

I

M

N

T

R

R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

A

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

I

R

A

M

H

H

G

G

E

E

I

I

F

F

V

V

L

P

D

K

M

I

G

P

K

S

P

-

V

M

K

K

I

M

V

T

D

D

L

L

A

A

R

K

D

A

L

L

I

A

T

-

L

-

S

-

G

-

Y

-

R

-

P

P

D

N

E

T

D

P

I

T

K

K

I

I

E

-

Y

-

T

I

G

G

L

I

R

R

P

P

G

G

E

E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

M

P

N

K

E

D

L

E

A

S

L

H

T

L

S

A

T

L

E

E

H

F

K

E

K

D

I

F

F

F

V

I

E

I

K

Q

P

P

TGSF 19:22 (≠ GGSI 293:296) binding NADP(+)
SRDELK 43:48 (≠ YENNVY 318:323) binding NADP(+)
DV 67:68 (≠ SI 344:345) binding NADP(+)
A87 (= A366) binding NADP(+)
K91 (= K370) binding NADP(+)
LS 129:130 (≠ IS 408:409) binding NADP(+)
K133 (= K412) mutation K->A,E: Loss of activity.
Y141 (≠ M420) binding NADP(+)
K145 (= K424) binding NADP(+)
VVGSR 174:178 (≠ VLGSN 450:454) binding NADP(+)

2gnaA Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-gal (see paper)
35% identity, 47% coverage: 282:571/618 of query aligns to 4:283/329 of 2gnaA

query
sites
2gnaA

I

L

K

D

D

N

K

Q

I

T

I

I

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

A

L

R

D

F

T

Y

T

P

N

K

A

Q

K

L

K

L

I

I

I

L

V

D

-

I

Y

Y
x
S

E
x
R

N
x
D

N

E

V

L

Y
x
K

D

Q

V

S

Q

E

M

M

E

A

L

M

N

E

Y

F

N

N

H

D

P

P

E

R

V

M

N

-

K

-

K

R

V

F

I

F

I

I

A

G

S
x
D

I
x
V

R

R

D

D

D

L

D

E

R

R

L

L

N

N

E

-

I

-

F

Y

S

A

H

L

Y

E

K

G

P

V

D

D

V

I

V

C

F

I

H

H

A
|
A

H

L

K
|
K

H

H

V

V

P

P

L

I

M

A

E

E

M

Y

N

N

P

P

A

L

E

E

A

C

V

I

K

K

N

T

N

N

I

I

I

M

G

G

T

A

Y

S

N

N

V

V

L

I

K

N

C

A

C

C

N

L

D

K

Y

N

N

A

V

I

K

S

K

Q

F

V

V

I

Q

A

I
x
L

S
|
S

T

T

D

D

K

K

A

A

V

A

N

N

P

P

T

I

N

N

I

L

M
x
Y

G

G

A

A

T

T

K
|
K

R

-

F

L

C

C

-

S

-

D

E

K

M

L

M

F

V

V

Q

S

A

A

-

N

F

F

D

K

A

G

V

S

S

S

K

Q

T

T

E

Q

Y

F

V

S

A

V

V

V

R

R

F
x
Y

G
|
G

N

N

V
|
V

L

V

G

G

S

S

N

R

G

G

S
|
S

V
|
V

I

V

P

P

L

F

F

F

K

K

Q

L

I

V

S

Q

H

N

-

K

G

A

G

S

P

E

V

I

T

P

V

I

T
|
T

D

D

P

I

K

R

I
x
M

N

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

A

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

I

R

A

M

H

H

G

G

E

E

I

I

F

F

V

V

L

P

D

K

M

I

G

P

K

S

P

-

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

D

A

L

L

I

A

T

-

L

-

S

-

G

-

Y

-

R

-

P

P

D

N

E

T

D

P

I

T

K

K

I

I

E

-

Y

-

T

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

M

P

N

K

E

D

L

E

A

S

L

H

T

L

S

A

T

L

E

E

H

F

K

E

K

D

I

F

F

F

V

I

E

I

K

Q

P

P

binding galactose-uridine-5'-diphosphate: K87 (= K370), S176 (= S456), V177 (= V457), T195 (= T474), M199 (≠ I478), R201 (= R480), M235 (≠ I515), R254 (= R542), E257 (= E545)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G291), T15 (≠ G293), G16 (= G294), S17 (= S295), F18 (≠ I296), S39 (≠ Y318), R40 (≠ E319), D41 (≠ N320), K44 (≠ Y323), D63 (≠ S344), V64 (≠ I345), A83 (= A366), A84 (= A367), A85 (= A368), K87 (= K370), L125 (≠ I408), S126 (= S409), Y137 (≠ M420), K141 (= K424), Y167 (≠ F447), G168 (= G448), V170 (= V450)

2gn9A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glc (see paper)
35% identity, 47% coverage: 282:571/618 of query aligns to 4:283/329 of 2gn9A

query
sites
2gn9A

I

L

K

D

D

N

K

Q

I

T

I

I

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

A

L

R

D

F

T

Y

T

P

N

K

A

Q

K

L

K

L

I

I

I

L

V

D

-

I

Y

Y

S

E
x
R

N
x
D

N

E

V

L

Y
x
K

D

Q

V

S

Q

E

M

M

E

A

L

M

N

E

Y

F

N

N

H

D

P

P

E

R

V

M

N

-

K

-

K

R

V

F

I

F

I

I

A

G

S
x
D

I
x
V

R

R

D

D

D

L

D

E

R

R

L

L

N

N

E

-

I

-

F

Y

S

A

H

L

Y

E

K

G

P

V

D

D

V

I

V

C

F

I

H

H

A
|
A

H

L

K
|
K

H

H

V

V

P

P

L

I

M

A

E

E

M

Y

N

N

P

P

A

L

E

E

A

C

V

I

K

K

N

T

N

N

I

I

I

M

G

G

T

A

Y

S

N

N

V

V

L

I

K

N

C

A

C

C

N

L

D

K

Y

N

N

A

V

I

K

S

K

Q

F

V

V

I

Q

A

I
x
L

S
|
S

T
|
T

D

D

K
|
K

A

A

V

A

N

N

P

P

T

I

N

N

I

L

M
x
Y

G

G

A

A

T

T

K
|
K

R

-

F

L

C

C

-

S

-

D

E

K

M

L

M

F

V

V

Q

S

A

A

-

N

F

F

D

K

A

G

V

S

S

S

K

Q

T

T

E

Q

Y

F

V

S

A

V

V

V

R

R

F
x
Y

G
|
G

N
|
N

V
|
V

L

V

G

G

S

S

N
x
R

G

G

S
|
S

V
|
V

I

V

P

P

L

F

F

F

K

K

Q

L

I

V

S

Q

H

N

-

K

G

A

G

S

P

E

V

I

T
x
P

V

I

T
|
T

D

D

P

I

K

R

I
x
M

N

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

A

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

I

R

A

M

H

H

G

G

E

E

I

I

F

F

V

V

L

P

D

K

M

I

G

P

K

S

P

-

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

D

A

L

L

I

A

T

-

L

-

S

-

G

-

Y

-

R

-

P

P

D

N

E

T

D

P

I

T

K

K

I

I

E

-

Y

-

T

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

M

P

N

K

E

D

L

E

A

S

L

H

T

L

S

A

T

L

E

E

H

F

K

E

K

D

I

F

F

F

V

I

E

I

K

Q

P

P

binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G291), T15 (≠ G293), G16 (= G294), S17 (= S295), F18 (≠ I296), R40 (≠ E319), D41 (≠ N320), K44 (≠ Y323), D63 (≠ S344), V64 (≠ I345), A83 (= A366), A84 (= A367), A85 (= A368), K87 (= K370), L125 (≠ I408), S126 (= S409), K141 (= K424), Y167 (≠ F447), G168 (= G448), V170 (= V450), R174 (≠ N454)
binding uridine-5'-diphosphate-glucose: K87 (= K370), T127 (= T410), K129 (= K412), Y137 (≠ M420), N169 (= N449), S176 (= S456), V177 (= V457), P193 (≠ T472), T195 (= T474), M199 (≠ I478), R201 (= R480), M235 (≠ I515), R254 (= R542), E257 (= E545)

2gn6A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glcnac (see paper)
35% identity, 47% coverage: 282:571/618 of query aligns to 4:283/329 of 2gn6A

query
sites
2gn6A

I

L

K

D

D

N

K

Q

I

T

I

I

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

A

L

R

D

F

T

Y

T

P

N

K

A

Q

K

L

K

L

I

I

I

L

V

D

-

I

Y

Y

S

E
x
R

N
x
D

N

E

V

L

Y
x
K

D

Q

V

S

Q

E

M

M

E

A

L

M

N

E

Y

F

N

N

H

D

P

P

E

R

V

M

N

-

K

-

K

R

V

F

I

F

I

I

A

G

S
x
D

I
x
V

R

R

D

D

D

L

D

E

R

R

L

L

N

N

E

-

I

-

F

Y

S

A

H

L

Y

E

K

G

P

V

D

D

V

I

V

C

F

I

H

H

A

A

A
|
A

H

L

K
|
K

H

H

V

V

P

P

L

I

M

A

E

E

M

Y

N

N

P

P

A

L

E

E

A

C

V

I

K

K

N

T

N

N

I

I

I

M

G

G

T

A

Y

S

N

N

V

V

L

I

K

N

C

A

C

C

N

L

D

K

Y

N

N

A

V

I

K

S

K

Q

F

V

V

I

Q

A

I

L

S
|
S

T

T

D
|
D

K
|
K

A

A

V

A

N

N

P

P

T

I

N

N

I

L

M
x
Y

G

G

A

A

T

T

K
|
K

R

-

F

L

C

C

-

S

-

D

E

K

M

L

M

F

V

V

Q

S

A

A

-

N

F

F

D

K

A

G

V

S

S

S

K

Q

T

T

E

Q

Y

F

V

S

A

V

V

V

R

R

F
x
Y

G
|
G

N
|
N

V
|
V

L

V

G

G

S
|
S

N
x
R

G
|
G

S
|
S

V
|
V

I

V

P

P

L

F

F

F

K

K

Q

L

I

V

S

Q

H

N

-

K

G

A

G

S

P

E

V

I

T
x
P

V
x
I

T

T

D

D

P

I

K

R

I
x
M

N

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

A

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

I

R

A

M

H

H

G

G

E

E

I

I

F

F

V

V

L

P

D

K

M

I

G

P

K

S

P

-

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

D

A

L

L

I

A

T

-

L

-

S

-

G

-

Y

-

R

-

P

P

D

N

E

T

D

P

I

T

K

K

I

I

E

-

Y

-

T

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

M

P

N

K

E

D

L

E

A

S

L

H

T

L

S

A

T

L

E

E

H

F

K

E

K

D

I

F

F

F

V

I

E

I

K

Q

P

P

binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G291), T15 (≠ G293), G16 (= G294), S17 (= S295), F18 (≠ I296), R40 (≠ E319), D41 (≠ N320), K44 (≠ Y323), D63 (≠ S344), V64 (≠ I345), A84 (= A367), A85 (= A368), K87 (= K370), S126 (= S409), Y137 (≠ M420), K141 (= K424), Y167 (≠ F447), G168 (= G448), V170 (= V450), S173 (= S453), R174 (≠ N454)
binding uridine-diphosphate-n-acetylglucosamine: K87 (= K370), D128 (= D411), K129 (= K412), N169 (= N449), G175 (= G455), S176 (= S456), V177 (= V457), P193 (≠ T472), I194 (≠ V473), M199 (≠ I478), R201 (= R480), M235 (≠ I515), R254 (= R542), E257 (= E545)

2gn4A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADPH and udp-glcnac (see paper)
35% identity, 47% coverage: 282:571/618 of query aligns to 4:283/329 of 2gn4A

query
sites
2gn4A

I

L

K

D

D

N

K

Q

I

T

I

I

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

K

E

C

L

F

C

V

R

R

Q

K

I

V

A

L

R

D

F

T

Y

T

P

N

K

A

Q

K

L

K

L

I

I

I

L

V

D

-

I

Y

Y
x
S

E
x
R

N
x
D

N

E

V

L

Y
x
K

D

Q

V

S

Q

E

M

M

E

A

L

M

N

E

Y

F

N

N

H

D

P

P

E

R

V

M

N

-

K

-

K

R

V

F

I

F

I

I

A

G

S
x
D

I
x
V

R

R

D

D

D

L

D

E

R

R

L

L

N

N

E

-

I

-

F

Y

S

A

H

L

Y

E

K

G

P

V

D

D

V

I

V

C

F

I

H

H

A
|
A

H

L

K
|
K

H

H

V

V

P

P

L

I

M

A

E

E

M

Y

N

N

P

P

A

L

E

E

A

C

V

I

K

K

N
x
T

N

N

I

I

I

M

G

G

T

A

Y

S

N

N

V

V

L

I

K

N

C

A

C

C

N

L

D

K

Y

N

N

A

V

I

K

S

K

Q

F

V

V

I

Q

A

I
x
L

S
|
S

T
|
T

D
|
D

K
|
K

A

A

V

A

N

N

P

P

T

I

N

N

I

L

M
x
Y

G

G

A

A

T

T

K
|
K

R

-

F

L

C

C

-

S

-

D

E

K

M

L

M

F

V

V

Q

S

A

A

-

N

F

F

D

K

A

G

V

S

S

S

K

Q

T

T

E

Q

Y

F

V

S

A

V

V

V

R

R

F
x
Y

G
|
G

N
|
N

V
|
V

L

V

G

G

S
|
S

N
x
R

G

G

S
|
S

V
|
V

I

V

P

P

L

F

F

F

K

K

Q

L

I

V

S

Q

H

N

-

K

G

A

G

S

P

E

V

I

T
x
P

V

I

T
|
T

D

D

P

I

K

R

I
x
M

N

T

R
|
R

F

F

F

W

M

I

T

T

I

L

P

D

E

E

A

G

A

V

Q

S

L

F

V

V

I

L

Q

K

A

S

G

L

A

K

I

R

A

M

H

H

G

G

E

E

I

I

F

F

V

V

L

P

D

K

M

I

G

P

K

S

P

-

V

M

K

K

I
x
M

V

T

D

D

L

L

A

A

R

K

D

A

L

L

I

A

T

-

L

-

S

-

G

-

Y

-

R

-

P

P

D

N

E

T

D

P

I

T

K

K

I

I

E

-

Y

-

T

I

G

G

L

I

R
|
R

P

P

G

G

E
|
E

K

K

L

L

Y

H

E

E

E

V

L

M

L

I

M

P

N

K

E

D

L

E

A

S

L

H

T

L

S

A

T

L

E

E

H

F

K

E

K

D

I

F

F

F

V

I

E

I

K

Q

P

P

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G13 (= G291), T15 (≠ G293), G16 (= G294), S17 (= S295), F18 (≠ I296), S39 (≠ Y318), R40 (≠ E319), D41 (≠ N320), K44 (≠ Y323), D63 (≠ S344), V64 (≠ I345), A83 (= A366), A84 (= A367), A85 (= A368), K87 (= K370), T102 (≠ N385), L125 (≠ I408), S126 (= S409), T127 (= T410), Y137 (≠ M420), K141 (= K424), Y167 (≠ F447), G168 (= G448), V170 (= V450), S173 (= S453), R174 (≠ N454)
binding uridine-diphosphate-n-acetylglucosamine: K87 (= K370), T127 (= T410), D128 (= D411), K129 (= K412), Y137 (≠ M420), N169 (= N449), S176 (= S456), V177 (= V457), P193 (≠ T472), T195 (= T474), M199 (≠ I478), R201 (= R480), M235 (≠ I515), R254 (= R542), E257 (= E545)

3w1vA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with inihibitor (see paper)
41% identity, 44% coverage: 284:555/618 of query aligns to 13:276/347 of 3w1vA

query
sites
3w1vA

D

D

K

K

I

I

I

L

L

L

V

I

T

T

G

G

G

T

G

G

S

S

I

F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

A

L

R

D

F

S

Y

N

P

I

K

K

Q

E

L

I

L

R

I

I

L

F

D

S

I

R

Y

D

E

E

N

K

V

Q

Y

D

D

D

V

I

Q

R

M

K

E

K

L

-

N

-

Y

Y

N

N

H

N

P

S

E

K

V

L

N

-

K

-

K

K

V

F

I

Y

I

I

A

G

S

D

I

V

R

R

D

D

D

S

D

Q

R

S

L

V

N

E

E

T

I

A

F

M

S

R

H

D

Y

V

K

-

P

-

D

D

V

Y

V

V

F

F

H

H

A

A

H

L

K
|
K

H
x
Q

V

V

P

P

L

S

M

C

E

E

M

F

N

F

P

P

A

V

E

E

A

A

V

V

K

K

N

T

N

N

I

I

G

G

T

T

Y

E

N

N

V

V

L

L

K

Q

C

S

C

A

N

I

D

H

Y

Q

N

N

V

V

K

K

F

V

V

I

Q

C

I

L

S

S

T

T

D

D

K

K

A

A

V

A

N

Y

P

P

T

I

N

N

I

A

M

M

G

G

A

I

T

S

K

K

R

-

F

-

C

-

E

A

M

M

V

E

Q

K

A

V

F

F

D

V

A

A

V

K

S

S

K

R

-

N

-

I

-

R

-

S

-

E

-

Q

T

T

E

L

Y

I

V

C

A

G

V

T

R

R

F

Y

G

G

N
|
N

V

V

L

M

G

A

S
|
S

N
x
R

G

G

S
|
S

V
|
V

I

I

P

P

L
|
L

F

F

K

I

K

D

Q

K

I

I

S

K

H

A

G

G

G

E

P

P

V

L

T
|
T

V
x
I

T
|
T

D

D

P

P

K

D

I
x
M

N

T

R
|
R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

A

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

I

H

A

A

H

E

G

T

G

G

E

D

I

I

F

M

V

V

L

-

D

-

M

Q

G

K

K

A

P

P

V

S

K

S

I

T

V
|
V

-

G

D

D

L

L

A

A

R

T

D

A

L

L

I

L

T

E

L

L

S

F

G

-

Y

-

R

-

P

-

D

E

E

A

D

D

I

N

K

A

I

I

E

E

Y

I

T

I

G

G

L

T

R
|
R

P

H

G

G

E
|
E

K

K

L

K

Y

A

E

E

E

T

L

L

M

T

N

R

E

E

binding [(2R,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,4-bis(oxidanyl)oxan-2-yl]methylimino-azanylidene-azanium: K93 (= K370), Q94 (≠ H371), N175 (= N449), S179 (= S453), R180 (≠ N454), S182 (= S456), V183 (= V457), L186 (= L460), T198 (= T472), I199 (≠ V473), T200 (= T474), M204 (≠ I478), R206 (= R480), V240 (= V516), R263 (= R542), E266 (= E545)

4g5hA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with by-product (see paper)
41% identity, 44% coverage: 284:555/618 of query aligns to 13:276/346 of 4g5hA

query
sites
4g5hA

D

D

K

K

I

I

I

L

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

A

L

R

D

F

S

Y

N

P

I

K

K

Q

E

L

I

L

R

I

I

L

F

D
x
S

I
x
R

Y
x
D

E

E

N

K

N
x
K

V

Q

Y

D

D

D

V

I

Q

R

M

K

E

K

L

-

N

-

Y

Y

N

N

H

N

P

S

E

K

V

L

N

-

K

-

K

K

V

F

I

Y

I

I

A

G

S
x
D

I
x
V

R

R

D

D

D

S

D

Q

R

S

L

V

N

E

E

T

I

A

F

M

S

R

H

D

Y

V

K

-

P

-

D

D

V

Y

V

V

F

F

H

H

A
|
A

H

L

K
|
K

H
x
Q

V
|
V

P

P

L

S

M

C

E

E

M

F

N

F

P

P

A

V

E

E

A

A

V

V

K

K

N

T

N

N

I

I

G

G

T

T

Y

E

N

N

V

V

L

L

K

Q

C

S

C

A

N

I

D

H

Y

Q

N

N

V

V

K

K

F

V

V

I

Q

C

I
x
L

S

S

T
|
T

D

D

K
|
K

A

A

V

A

N

Y

P

P

T

I

N

N

I

A

M

M

G

G

A

I

T

S

K
|
K

R

-

F

-

C

-

E

A

M

M

V

E

Q

K

A

V

F

F

D

V

A

A

V

K

S

S

K

R

-

N

-

I

-

R

-

S

-

E

-

Q

T

T

E

L

Y

I

V

C

A

G

V

T

R

R

F
x
Y

G

G

N
|
N

V

V

L

M

G

A

S

S

N

R

G

G

S
|
S

V
|
V

I

I

P

P

L
|
L

F

F

K

I

K

D

Q

K

I

I

S

K

H

A

G

G

G

E

P

P

V

L

T
|
T

V

I

T
|
T

D

D

P

P

K

D

I
x
M

N

T

R

R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

A

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

I

H

A

A

H

E

G

T

G

G

E

D

I

I

F

M

V

V

L

-

D

-

M

Q

G

K

K

A

P

P

V

S

K

S

I

T

V
|
V

-

G

D

D

L

L

A

A

R

T

D

A

L

L

I

L

T

E

L

L

S

F

G

-

Y

-

R

-

P

-

D

E

E

A

D

D

I

N

K

A

I

I

E

E

Y

I

T

I

G

G

L

T

R
|
R

P

H

G

G

E
|
E

K

K

L

K

Y

A

E

E

E

T

L

L

M

T

N

R

E

E

binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G291), T22 (≠ G293), G23 (= G294), S24 (= S295), F25 (≠ I296), S45 (≠ D316), R46 (≠ I317), D47 (≠ Y318), K50 (≠ N321), D69 (≠ S344), V70 (≠ I345), A89 (= A366), A90 (= A367), A91 (= A368), K93 (= K370), L131 (≠ I408), T133 (= T410), K147 (= K424), Y173 (≠ F447)
binding [(2R,3R,4R,6R)-3-acetamido-6-methyl-4-oxidanyl-5-oxidanylidene-oxan-2-yl] [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Q94 (≠ H371), V95 (= V372), K135 (= K412), N175 (= N449), S182 (= S456), V183 (= V457), L186 (= L460), T198 (= T472), T200 (= T474), M204 (≠ I478), V240 (= V516), R263 (= R542), E266 (= E545)

Sites not aligning to the query:

binding [(2R,3R,4R,6R)-3-acetamido-6-methyl-4-oxidanyl-5-oxidanylidene-oxan-2-yl] [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 278, 313, 314, 315, 316, 320

3vvcA Crystal structure of capsular polysaccharide synthesizing enzyme cape , k126e, in apo form (see paper)
40% identity, 44% coverage: 284:555/618 of query aligns to 5:268/318 of 3vvcA

query
sites
3vvcA

D

D

K

K

I

I

I

L

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S

S

I
x
F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

A

L

R

D

F

S

Y

N

P

I

K

K

Q

E

L

I

L

R

I

I

L

F

D
x
S

I
x
R

Y
x
D

E

E

N

K

N
x
K

V

Q

Y

D

D

D

V

I

Q

R

M

K

E

K

L

-

N

-

Y

Y

N

N

H

N

P

S

E

K

V

L

N

-

K

-

K

K

V

F

I

Y

I

I

A

G

S
x
D

I
x
V

R
|
R

D

D

D

S

D

Q

R

S

L

V

N

E

E

T

I

A

F

M

S

R

H

D

Y

V

K

-

P

-

D

D

V

Y

V

V

F

F

H

H

A
|
A

H

L

K
|
K

H

Q

V

V

P

P

L

S

M

C

E

E

M

F

N

F

P

P

A

V

E

E

A

A

V

V

K

K

N

T

N

N

I

I

G

G

T

T

Y

E

N

N

V

V

L

L

K

Q

C

S

C

A

N

I

D

H

Y

Q

N

N

V

V

K

K

F

V

V

I

Q

C

I

L

S
|
S

T
|
T

D

D

K

E

A

A

V

A

N

Y

P

P

T

I

N

N

I

A

M

M

G

G

A

I

T

S

K
|
K

R

-

F

-

C

-

E

A

M

M

V

E

Q

K

A

V

F

F

D

V

A

A

V

K

S

S

K

R

-

N

-

I

-

R

-

S

-

E

-

Q

T

T

E

L

Y

I

V

C

A

G

V

T

R

R

F
x
Y

G
|
G

N

N

V

V

L

M

G

A

S

S

N

R

G

G

S

S

V

V

I

I

P

P

L

L

F

F

K

I

K

D

Q

K

I

I

S

K

H

A

G

G

G

E

P

P

V

L

T

T

V

I

T

T

D

D

P

P

K

D

I

M

N

T

R

R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

A

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

I

H

A

A

H

E

G

T

G

G

E

D

I

I

F

M

V

V

L

-

D

-

M

Q

G

K

K

A

P

P

V

S

K

S

I

T

V

V

-

G

D

D

L

L

A

A

R

T

D

A

L

L

I

L

T

E

L

L

S

F

G

-

Y

-

R

-

P

-

D

E

E

A

D

D

I

N

K

A

I

I

E

E

Y

I

T

I

G

G

L

T

R

R

P

H

G

G

E

E

K

K

L

K

Y

A

E

E

E

T

L

L

M

T

N

R

E

E

binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G291), T14 (≠ G293), G15 (= G294), F17 (≠ I296), S37 (≠ D316), R38 (≠ I317), D39 (≠ Y318), K42 (≠ N321), D61 (≠ S344), V62 (≠ I345), R63 (= R346), A81 (= A366), A82 (= A367), A83 (= A368), K85 (= K370), S124 (= S409), T125 (= T410), K139 (= K424), Y165 (≠ F447), G166 (= G448)

4j2oC Crystal structure of NADP-bound wbjb from a. Baumannii community strain d1279779 (see paper)
39% identity, 44% coverage: 283:555/618 of query aligns to 3:268/316 of 4j2oC

query
sites
4j2oC

K

K

D

D

K

K

I

V

I

L

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

N

E

A

L

V

C

L

R

K

Q

R

I

F

A

L

R

E

F

T

Y

D

P

I

K

K

Q

E

L

I

L

R

I

I

L

F

D
x
S

I
x
R

Y
x
D

E

E

N

K

N
x
K

V

Q

Y

D

D

D

V

-

Q

-

M

M

E

R

L

K

N

K

Y

Y

N

H

H

S

P

A

E

K

V

L

N

-

K

-

K

K

V

F

I

Y

I

I

A

G

S
x
D

I
x
V

R

R

D

D

D

-

R

-

L

Y

N

N

E

S

I

I

F

L

S

N

H

A

Y

T

K

R

P

G

-

V

D

D

V

Y

V

I

F

Y

H

H

A
|
A

H

L

K
|
K

H

Q

V

V

P

P

L

S

M

C

E

E

M

F

N

H

P

P

A

M

E

E

A

A

V

V

K

K

N
x
T

N

N

I

V

I

L

G

G

T

T

Y

E

N

N

V

V

L

L

K

E

C

A

N

I

D

Q

Y

N

N

H

V

V

K

K

K

R

F

V

V

V

Q

C

I
x
L

S

S

T

T

D

D

K

K

A

A

V

V

N

Y

P

P

T

I

N

N

I

A

M

M

G

G

A

I

T

S

K
|
K

R

A

F

M

C

M

E

E

-

K

M

V

M

M

V

V

Q

A

A

K

F

S

D

R

A

N

V

L

S

E

K

G

T

L

E

D

Y

T

V

V

-

I

-

C

A

G

V

T

R

R

F
x
Y

G

G

N

N

V

V

L

M

G

A

S

S

N

R

G

G

S

S

V

V

I

I

P

P

L

L

F

F

K

V

K

D

Q

Q

I

I

S

R

H

Q

G

G

G

K

P

P

V

L

T

T

V

I

T

T

D

D

P

P

K

N

I

M

N

T

R

R

F

F

F

M

M

M

T

T

I

L

P

E

E

D

A

A

A

V

Q

D

L

L

V

V

I

L

Q

Y

A

A

G

F

A

E

I

H

A

G

H

E

G

N

G

G

E

D

I

I

F

F

V

V

L

-

D

Q

M

K

G

A

K

P

P

A

V

A

K

T

I

I

V

A

D

V

L

L

A

A

R

E

D

A

L

L

I

K

T

Q

L

L

S

L

G

N

Y

V

R

-

P

-

D

-

E

E

D

D

I

H

K

P

I

I

E

S

Y

I

T

M

G

G

L

T

R

R

P

H

G

G

E

E

K

K

L

A

Y

F

E

E

E

A

L

L

M

S

N

R

E

E

binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G291), T13 (≠ G293), G14 (= G294), S15 (= S295), F16 (≠ I296), S36 (≠ D316), R37 (≠ I317), D38 (≠ Y318), K41 (≠ N321), D60 (≠ S344), V61 (≠ I345), A80 (= A366), A81 (= A367), A82 (= A368), K84 (= K370), T99 (≠ N385), L122 (≠ I408), K138 (= K424), Y164 (≠ F447)

3vvbA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in apo form (see paper)
34% identity, 41% coverage: 284:534/618 of query aligns to 3:230/270 of 3vvbA

query
sites
3vvbA

D

D

K

K

I

I

I

L

L

L

V

I

T

T

G
|
G

G

G

G
x
T

G
|
G

S
|
S

I
x
F

G

G

S

N

E

A

L

V

C

M

R

K

Q

R

I

F

A

L

R

D

F

S

Y

N

P

I

K

K

Q

E

L

I

L

R

I

I

L

F

D
x
S

I
x
R

Y
x
D

E

E

N

K

N
x
K

V

Q

Y

D

D

D

V

I

Q

R

M

K

E

K

L

-

N

-

Y

Y

N

N

H

N

P

S

E

K

V

L

N

-

K

-

K

K

V

F

I

Y

I

I

A

G

S
x
D

I
x
V

R

R

D

D

D

S

D

Q

R

S

L

V

N

E

E

T

I

A

F

M

S

R

H

D

Y

V

K

-

P

-

D

D

V

Y

V

V

F

F

H

H

A

A

A
|
A

H

L

K
|
K

H

Q

V

V

P

P

L

S

M

C

E

E

M

F

N

F

P

P

A

V

E

E

A

A

V

V

K

K

N

T

N

N

I

I

G

G

T

T

Y

E

N

N

V

V

L

L

K

Q

C

S

C

A

N

I

D

H

Y

Q

N

N

V

V

K

K

F

V

V

I

Q

C

I
x
L

S

S

T
|
T

D

D

K

K

A

A

V

A

N

Y

P

P

T

I

N

N

I

A

M

M

G

G

A

I

T

S

K
|
K

R

-

F

-

C

-

E

A

M

M

V

E

Q

K

A

V

F

F

D

V

A

A

V

K

S

S

K

R

-

N

-

I

-

R

-

S

-

E

-

Q

T

T

E

L

Y

I

V

C

A

G

V

T

R

R

F
x
Y

G
|
G

N

N

V

V

L

M

G

-

S

-

N

-

G

-

S

-

V

-

I

-

P

-

L

-

F

-

K

-

Q

-

I

-

S

-

H

-

G

-

P

-

V

-

T

-

V

-

T

-

D

-

P

P

K

D

I

M

N

T

R

R

F

F

F

L

M

M

T

S

I

L

P

E

E

D

A

A

A

V

Q

E

L

L

V

V

I

V

Q

H

A

A

G

F

A

K

I

H

A

A

H

E

G

T

G

G

E

D

I

I

F

M

V

V

L

-

D

-

M

Q

G

K

K

A

P

P

V

S

K

S

I

T

V

V

D

G

-

D

-

L

-

A

-

T

-

A

-

L

L

L

A

A

R

E

D

T

L

L

I

L

T

T

L

R

S

E

G

E

Y

Y

R

A

P

Q

D

C

E

E

D

D

I

M

binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G291), T12 (≠ G293), G13 (= G294), S14 (= S295), F15 (≠ I296), S35 (≠ D316), R36 (≠ I317), D37 (≠ Y318), K40 (≠ N321), D59 (≠ S344), V60 (≠ I345), A80 (= A367), A81 (= A368), K83 (= K370), L121 (≠ I408), T123 (= T410), K137 (= K424), Y163 (≠ F447), G164 (= G448)

4tqgA Crystal structure of megavirus udp-glcnac 4,6-dehydratase, 5-epimerase mg534 (see paper)
30% identity, 45% coverage: 282:560/618 of query aligns to 3:254/297 of 4tqgA

query
sites
4tqgA

I

I

K

N

D

D

K

K

I

T

I

I

L

M

V

I

T

F

G
|
G

G

G

G
x
S

G
|
G

S
|
S

I
x
L

G

G

S

N

E

R

L

L

C

I

R

E

Q

T

I

-

A

-

R

-

F

-

Y

Y

P

I

K

N

Q

N

L

N

L

I

I

I

L

V

D

N

I

Y

Y

S

E
x
R

N
x
D

N

E

V

S

Y

K

D

H

V

W

Q

S

M

M

E

E

L

L

N

K

Y

Y

N

K

H

S

P

D

E

K

V

L

N

-

K

-

K

K

V

N

I

I

A

G

S
x
D

I
|
I

R

R

D

D

D

F

D

E

R

K

L

V

N

Q

E

Q

I

S

F

I

S

M

H

R

Y

I

K

N

P

P

D

D

V

I

F

I

H

I

A
|
A

H

L

K
|
K

H

H

V

I

P

D

L

R

M

C

E

E

M

Y

N

E

P

I

A

N

E

E

A

C

V

L

K

D

N

T

N

N

I

I

I

K

G

G

T

L

Y

Q

N

N

V

V

L

L

K

K

C

V

C

T

-

E

-

I

-

N

-

R

-

S

N

N

D

L

Y

S

N

N

V

L

K

K

K

A

F

V

V

C

Q

F

I
x
V

S

S

T

T

D

D

K

K

A

A

V

C

N

S

P

P

T

V

N

N

I

S

M
x
Y

G

G

A

M

T

S

K
|
K

R

A

F

I

C

C

E

E

-

T

M

L

M

V

V

V

Q

E

A

K

F

S

D

K

A

Y

V

I

S

K

K

D

T

I

E

K

Y

Y

V

V

A

C

V

V

R

R

F
x
Y

G
|
G

N

N

V
|
V

L

L

G

N

S

-

N

-

G

-

S

-

V

-

I

-

P

-

L

-

F

-

K

-

Q

-

I

-

S

-

H

-

G

-

P

-

V

F

T

T

V

L

T

T

D

H

P

T

K

S

I

M

N

T

R

R

F

F

F

I

M

M

T

T

I

L

P

D

E

D

A

S

A

V

Q

K

L

L

V

I

I

E

Q

Y

A

A

G

I

A

I

I

N

A

G

H

N

G

S

G

G

E

E

I

I

F

V

V

I

L

P

D

K

M

L

G

N

K

S

P

M

V

-

K

-

I

-

V

-

D

-

L

Y

A

I

R

K

D

D

L

M

I

I

T

E

L

L

S

F

G

A

Y

-

R

-

P

-

D

-

E

D

D

K

I

Y

K

P

I

I

E

V

Y

I

T

T

G

G

L

L

R

R

P

S

G

G

E

E

K

R

L

M

Y

Y

E

E

E

S

L

L

L

I

M

N

N

D

E

T

L

Q

A

S

L

M

T

K

S

T

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G291), S14 (≠ G293), G15 (= G294), S16 (= S295), L17 (≠ I296), R36 (≠ E319), D37 (≠ N320), D59 (≠ S344), I60 (= I345), A81 (= A366), A82 (= A367), A83 (= A368), K85 (= K370), V128 (≠ I408), Y140 (≠ M420), K144 (= K424), Y168 (≠ F447), G169 (= G448), V171 (= V450)

3pvzA Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
28% identity, 49% coverage: 261:562/618 of query aligns to 1:316/372 of 3pvzA

query
sites
3pvzA

N

S

I

I

E

L

D

S

L

L

L

I

G

G

R

R

-

D

-

T

-

E

-

L

-

F

-

H

E

Q

E

D

V

I

K

N

L

A

N

N

N

E

S

K

N

E

I

L

N

Q

K

S

Y

V

I

V

K

S

D

Q

K

S

I

R

I

F

L

L

V

V

T

L

G

G

G

A

G
|
G

S
|
S

I
|
I

G

G

S

Q

E

A

L

V

C

T

R

K

Q

E

I

I

A

F

R

K

F

R

Y

N

P

P

K

Q

Q

K

L

L

L

H

I

V

L

V

D
|
D

I
|
I

Y

S

E

E

N

N

-

M

-

V

-

E

-

L

V

V

Y

R

D

D

V

I

Q

R

M

S

E

S

L

F

N

G

Y

Y

N

I

H

N

P

G

E

D

V

F

N

Q

K

T

K

F

V

A

I

L

-
x
D

I
|
I

A

G

S

S

I

I

R

E

D

Y

D

D

D

A

R

F

L

I

N

K

E

-

I

-

F

-

S

A

H

D

Y

G

K

Q

P

Y

D

D

V

Y

V

V

F

L

H

N

A
x
L

A
x
S

A
|
A

H

L

K
|
K

H

H

V

V

P

R

L

-

M

S

E

E

M

K

N

D

P

P

-

F

-

T

-

L

A

M

E

R

A

M

V

I

K

D

N

V

N

N

I

V

I

F

G

N

T

T

Y

D

N

K

V

T

L

I

K

Q

C

Q

C

S

N

I

D

D

Y

A

N

G

V

A

K

K

F

Y

V

F

Q

C

I
x
V

S

S

T

T

D
|
D

K

K

A

A

V

A

N

N

P

P

T

V

N

N

I

M

M

M

G

G

A

A

T

S

K
|
K

R

R

F

I

C

M

E

E

M

M

M

F

V

L

Q

-

A

-

F

-

D

-

A

-

V

M

S

R

K

K

T

S

E

E

Y

E

V

I

A

A

V

I

-

S

-

T

-

A

R

R

F

F

G

A

N

N

V
|
V

L

A

G

F

S
|
S

N

D

G

G

S

S

V

L

I

L

P

H

L

G

F

F

K

N

K

Q

Q

R

I

I

S

Q

H

K

G

N

G

Q

P

P

V

I

T

-

V

V

T

A

D

P

P

N

K

D

I

I

N

K

R

R

F

Y

F

F

M

V

T

T

I

P

P

Q

E

E

A

S

A

G

Q

E

L

L

V

C

I

L

Q

M

A

S

G

C

A

I

I

F

A

G

H

E

G

N

G

R

E

D

I

I

F

F

V

F

L

P

D

K

M

L

G

S

K

E

P

A

V

L

K

H

I

L

V

I

-

S

-

F

-

A

D

D

L

I

A

A

R

V

D

K

L

Y

I

L

T

K

L

Q

S

L

G

G

Y

Y

R

E

P

P

-

H

-

L

-

C

-

E

D

S

E

E

D

D

I

E

K

A

I

R

E

E

Y

L

T

A

G

K

L

T

R

L

P

P

G

A

E

Q

K

G

L

K

Y

W

E

P

E

C

L

L

L

F

M

T

N

S

E

S

L

E

A

F

L

F

T

T

S

D

T

K

E

E

binding nicotinamide-adenine-dinucleotide: G40 (= G294), S41 (= S295), I42 (= I296), D62 (= D316), I63 (= I317), D92 (vs. gap), I93 (= I342), L114 (≠ A366), S115 (≠ A367), A116 (= A368), K118 (= K370), V158 (≠ I408), D161 (= D411), K174 (= K424), V198 (= V450), S201 (= S453)

Q9LPG6 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2; NDP-rhamnose synthase; Protein MUCILAGE-MODIFIED 4; Protein RHAMNOSE BIOSYNTHESIS 2; Rhamnose biosynthetic enzyme 2; AtRHM2; UDP-L-rhamnose synthase MUM4; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
28% identity, 41% coverage: 283:536/618 of query aligns to 7:281/667 of Q9LPG6

query
sites
Q9LPG6

K

K

D

P

K

K

I

N

I

I

L

L

V

I

T

T

G

G

G

A

G
|
G

S

F

I

I

G

A

S

S

E

H

L

V

C

A

R

N

Q

R

I

L

A

I

R

R

F

N

Y

Y

P

P

K

D

-

Y

Q
x
K

L

I

L

V

I

V

L

L

D

D

I

K

Y

L

E

D

N

Y

N

C

V

S

Y

D

D

L

V

K

Q

N

M

L

E

D

L

P

N

S

Y

F

N

S

H

S

P

P

E

-

V

-

N

N

K

F

K

K

V

F

I

V

I

K

A

G

S

D

I

I

R

A

D

S

D

D

R

L

L

V

N

N

E

Y

I

L

F

L

S

I

H

T

Y

E

K

N

P

I

D

D

V

T

V

I

F

M

H

H

A

F

A

A

H

Q

K

T

H

H

V

V

P
x
D

L

N

M

S

E

F

M

G

N

N

P

S

A

F

E

E

A

F

V

T

K

K

N

N

I

I

I

Y

G

G

T

T

Y

H

N

V

V

L

L

L

K

E

C

A

C

C

N

K

-

V

D

T

Y

G

N

Q

V

I

K

R

F

F

V

I

Q

H

I

V

S

S

T

T

D

D

K

E

A

V

V

Y

N

G

-

E

-

T

-

D

-

E

-

D

-

A

-

V

-

G

-

N

-

H

-

E

-

A

-

S

-

Q

-

L

P

P

T

T

N

N

I

P

M

Y

G

S

A

A

T

T

K
|
K

R

A

F

G

C

A

E

E

M

M

M

L

V

V

Q

M

A

A

F

Y

D

G

A

R

V

S

S

Y

K

G

T

L

E

P

Y

V

V

I

A

T

V

T

R

R

F

G

G

N

N

N

V

V

L

Y

G
|
G

S

P

N

N

G

Q

-

F

-

P

-

E

S

K

V

M

I

I

P

P

L

K

F

F

K

I

K

L

Q

L

I

A

S

M

H

S

G

G

G

K

P

P

V

L

T

P

V

I

T

H

D

G

P

D

K

G

I

S

N

N

-

V

R

R

F

S

F

Y

M

L

-

Y

-

C

-

E

T

D

I

V

P

A

E

E

A

A

A

F

Q

E

L

V

V

V

I

L

Q

H

A

K

G

G

A

E

I

I

A

G

H

H

G

-

E

-

I

V

F

Y

V

N

L

V

D

G

M

T

G

K

K

R

P

E

V

R

K

R

I

V

V

I

D

D

L

V

A

A

R

R

D

D

L

I

I

C

T

K

L

L

S

F

G

G

Y

K

R

D

P

P

D

E

E

S

D

S

I

I

K

Q

I

F

G18 (= G294) mutation to A: Abolishes dehydratase activity.
K36 (≠ Q311) mutation to A: Reduces dehydratase activity.
D96 (≠ P373) mutation to N: In mum4-1; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.
K165 (= K424) mutation to A: Abolishes dehydratase activity.
G193 (= G452) mutation to R: In mum4-2; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.

Sites not aligning to the query:

392 G→A: No effect on dehydratase activity.
413 K→A: No effect on dehydratase activity.
518 K→A: No effect on dehydratase activity.

3pvzB Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
29% identity, 39% coverage: 265:505/618 of query aligns to 3:236/300 of 3pvzB

query
sites
3pvzB

L

L

L

I

G

G

R

R

-

D

-

T

-

E

-

L

-

F

-

H

E

Q

E

D

V

I

K

N

L

A

N

N

N

E

S

K

N

E

I

L

N

Q

K

S

Y

V

I

V

K

S

D

Q

K

S

I

R

I

F

L

L

V

V

T

L

G

G

G

A

G
|
G

S
|
S

I
|
I

G

G

S

Q

E

A

L

V

C

T

R

K

Q

E

I

I

A

F

R

K

F

R

Y

N

P

P

K

Q

Q

K

L

L

L

H

I

V

L

V

D
|
D

I
|
I

Y

S

E

E

N

N

-

M

-

V

-

E

-

L

V

V

Y

R

D

D

V

I

Q

R

M

S

E

S

L

F

N

G

Y

Y

N

I

H

N

P

G

E

D

V

F

N

Q

K

T

K

F

V

A

I
x
L

-
x
D

I
|
I

A

G

S

S

I

I

R

E

D

Y

D

D

D

A

R

F

L

I

N

K

E

-

I

-

F

-

S

A

H

D

Y

G

K

Q

P

Y

D

D

V

Y

V

V

F

L

H

N

A
x
L

A
x
S

A
|
A

H

L

K
|
K

H

H

V

V

P

R

L

-

M

S

E

E

M

K

N

D

P

P

-

F

-

T

-

L

A

M

E

R

A

M

V

I

K

D

N

V

N

N

I

V

I

F

G

N

T

T

Y

D

N

K

V

T

L

I

K

Q

C

Q

C

S

N

I

D

D

Y

A

N

G

V

A

K

K

F

Y

V

F

Q

C

I

V

S

S

T

T

D
|
D

K

K

A

A

V

A

N

N

P

P

T

V

N

N

I

M

M

M

G

G

A

A

T

S

K
|
K

R

R

F

I

C

M

E

E

M

M

M

F

V

L

Q

-

A

-

F

-

D

-

A

-

V

M

S

R

K

K

T

S

E

E

Y

E

V

I

A

A

V

I

-

S

-

T

-

A

R

R

F

F

G

A

N
|
N

V
|
V

L

A

G

F

S
|
S

N

D

G

G

S

S

V

-

I

-

P

-

L

-

F

-

K

-

Q

R

I

I

S

Q

H

K

G

N

G

Q

P

P

V

I

T

-

V

-

T

-

D

-

P

-

K

-

I

-

N

-

R

R

F

Y

F

F

M

V

T

T

I

P

P

Q

E

E

A

S

A

G

Q

E

L

L

V

C

I

L

Q

M

A

S

G

C

A

I

I

F

A

G

H

E

G

N

G

R

E

D

I

I

F

F

binding nicotinamide-adenine-dinucleotide: G38 (= G294), S39 (= S295), I40 (= I296), D60 (= D316), I61 (= I317), L89 (≠ I341), D90 (vs. gap), I91 (= I342), L112 (≠ A366), S113 (≠ A367), A114 (= A368), K116 (= K370), D159 (= D411), K172 (= K424), N195 (= N449), V196 (= V450), S199 (= S453)

Q9SYM5 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1; Protein REPRESSOR OF LRX1 1; Rhamnose biosynthetic enzyme 1; AtRHM1; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
27% identity, 41% coverage: 285:535/618 of query aligns to 7:278/669 of Q9SYM5

query
sites
Q9SYM5

K

K

I

N

I

I

L

L

V

I

T

T

G

G

G

A

G

G

S

F

I

I

G

A

S

S

E

H

L

V

C

A

R

N

Q

R

I

L

A

I

R

R

F

S

Y

Y

P

P

K

D

-

Y

Q

K

L

I

L

V

I

V

L

L

D

D

I

K

-

L

-

D

Y

Y

E

C

N

S

N

N

V

L

Y

K

D

N

V

-

Q

-

M

-

E

-

L

L

N

N

Y

P

N

S

H

K

P

H

E

S

V

P

N

N

K

F

K

K

V

F

I

V

I

K

A

G

S

D

I

I

R

A

D

S

D

A

D

D

R

L

L

V

N

N

E

H

I

L

F

L

S

I

H

T

Y

E

K

G

P

I

D

D

V

T

V

I

F

M

H

H

A

F

A

A

H

Q

K

T

H

H

V

V

P

D

L

N

M

S

E

F

M

G

N

N

P

S

A

F

E

E

A

F

V

T

K

K

N

N

I

I

I

Y

G

G

T

T

Y

H

N

V

V

L

L

L

K

E

C

A

C

C

N

K

-

V

D

T

Y

G

N

Q

V

I

K

R

F

F

V

I

Q

H

I

V

S

S

T

T

D

D

K

E

A

V

V

Y

N

G

-

E

-

T

-

D

-

E

-

D

-

A

-

L

-

V

-

G

-

N

-

H

-

E

-

A

-

S

-

Q

-

L

P

P

T

T

N

N

I

P

M

Y

G

S

A

A

T

T

K

K

R

A

F

G

C

A

E

E

M

M

M

L

V

V

Q

M

A

A

F

Y

D

G

A

R

V

S

S

Y

K

G

T

L

E

P

Y

V

V

I

A

T

V

T

R

R

F

G

G

N

N

N

V

V

L

Y

G

G

S

P

N

N

G

Q

-

F

-

P

-

E

S

K

V

L

I

I

P

P

L

K

F

F

K

I

K

L

Q

L

I

A

S

M

H

R

G

G

G

Q

-

V

-

L

P

P

V

I

T

H

V

G

T

D

D

G

P

S

K

N

I

V

N

R

R

S

F

Y

F

L

M

Y

T

C

-

E

-

D

I

V

P

A

E

E

A

A

A

F

Q

E

L

V

V

V

I

L

Q

H

A

K

G

G

A

E

I

V

A

G

H

H

G

-

E

-

I

V

F

Y

V

N

L

I

D

G

M

T

G

K

K

K

P

E

V

R

K

R

I

V

V

N

D

D

L

V

A

A

R

K

D

D

L

I

I

C

T

K

L

L

S

F

G

N

Y

M

R

D

P

P

D

E

E

A

D

N

I

I

K

K

Sites not aligning to the query:

283 R→K: In rol1-2; Abolishes dehydratase activity in vitro (PubMed:16766693). Induces aberrant accumulation of flavonols leading to alterations in plant growth and cell shape formation (PubMed:18567791, PubMed:18757557).

6dntA Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
29% identity, 43% coverage: 282:545/618 of query aligns to 1:272/310 of 6dntA

query
sites
6dntA

I

M

K

K

D

D

K

K

I

N

I

V

L

V

V

V

T

T

G
|
G

G

G

G

L

G
|
G

S
x
F

I
|
I

G

G

S

S

E

H

L

I

C

V

R

D

Q

-

I

-

A

A

R

L

F

I

Y

D

P

D

K

N

Q

K

L

V

L

T

I

I

L

I

D
|
D

I

-

Y

-

E

-

N

-

N
|
N

V
x
L

Y
x
S

D
x
S

V
x
G

Q

K

M

M

E

E

L

-

N

N

Y

L

N

N

H

N

P

P

E

N

V

H

N

E

K

N

K

L

V

T

I

I

A

K

S

E

I
x
D

R
x
L

D

M

D

D

D

A

R

D

L

L

N

E

E

K

I

I

F

L

S

K

H

-

Y

-

K

D

P

K

D

D

V

Y

V

V

F

F

H

H

A
x
L

A
|
A

H

L

K
x
A

H

S

V

V

P

P

L

G

M

S

E

V

M

A

N

E

P

P

A

L

E

R

A

Y

V

N

K

Q

N

N

I

I

I

D

G

A

T

S

Y

L

N

K

V

L

L

F

K

I

C

A

C

C

N

K

D

N

Y

N

N

N

V

I

K

K

F

V

V

I

Q

-

I

F

S
|
S

T
x
S

D
x
S

K
x
S

A
|
A

V

V

N

Y

-

G

-

E

-

N

-

P

-

N

-

M

-

P

-

L

-

K

-

E

-

S

-

E

-

N

-

F

-

L

P

P

T

C

N

S

I

P

M
x
Y

G

A

A

A

T

Q

K
|
K

R

A

F

S

C

C

E

E

M

L

M

Y

V

L

Q

K

A

S

F

F

D

H

A

E

V

S

S

Y

K

G

T

L

E

D

Y

Y

V

V

A

A

V

L

R

R

F
x
Y

G
x
F

N
|
N

V
|
V

L

F

G

G

S

P

N

R

-

Q

-

D

-

E

-

N

-

S

-

P

-

Y

G

A

S
x
A

V
|
V

I

I

P

P

L
x
K

F

F

K

I

K

S

Q

A

I

I

S

L

H

N

G

G

-

E

G

S

P

P

V
|
V

T
x
I

V
x
Y

T

G

D

D

P

G

K
x
E

I
x
Q

N

S

R
|
R

F

D

F

F

M

I

T

Y

I

V

P

K

E

E

A

I

A

A

Q

K

L

A

V

N

I

I

Q

L

A

S

G

A

A

E

I

S

A

D

H

Y

G

N

G

G

E

V

I

I

F

N

V

V

L

A

D

-

M

L

G

G

K

K

P

S

V

M

-

T

-
x
I

-

N

K

R

I

L

V

F

D

E

L

I

A

I

R

S

D

D

L

V

I

L

T

-

L

-

S

-

G

-

Y

-

R

-

P

-

D

E

E

S

D

D

I

I

K

D

I

V

E

K

Y

Y

T

L

G

D

L

E

R
|
R

P

P

G

G

E
x
D

active site: S120 (≠ D411), S121 (≠ K412), A122 (= A413), Y144 (≠ M420), K148 (= K424), A187 (≠ S456)
binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: A80 (≠ K370), S120 (≠ D411), S121 (≠ K412), Y144 (≠ M420), F172 (≠ G448), N173 (= N449), A187 (≠ S456), V188 (= V457), K191 (≠ L460), V203 (= V471), I204 (≠ T472), Y205 (≠ V473), Q210 (≠ I478), R212 (= R480), I246 (vs. gap), R269 (= R542), D272 (≠ E545)
binding nicotinamide-adenine-dinucleotide: G10 (= G291), G13 (= G294), F14 (≠ S295), I15 (= I296), D33 (= D316), N34 (= N321), L35 (≠ V322), S36 (≠ Y323), S37 (≠ D324), G38 (≠ V325), D57 (≠ I345), L58 (≠ R346), L76 (≠ A366), A77 (= A367), A78 (= A368), A80 (≠ K370), S118 (= S409), S119 (≠ T410), Y144 (≠ M420), K148 (= K424), Y171 (≠ F447), V174 (= V450)
binding zinc ion: E209 (≠ K477)

Sites not aligning to the query:

binding zinc ion: 275

Query Sequence

>WP_039652482.1 NCBI__GCF_000816635.1:WP_039652482.1
MQKDKKLIIYDIALIIVSLYFAFLLRFDFSIPPEYMEFLKMSLIPVIGITLIFNRFFKLY
SSIWKYASIEELMSIIYSVSLSNAVFVVYSYIVSFRFLENRYYRFPYTVHIIFWILSVVT
LGGTRFLLRMFEQSAGEKGVKKKCKRLLIIGAGDASAMLIKEIKGHKNLNYDIVGLIDDD
SSKKKKFISGIEVLGGRADIPEICKRHRIEDIIIAIPSVDMHSKREIINICKSTACKLKT
LPGIYEIVDGKVNISKLRDVNIEDLLGREEVKLNNSNINKYIKDKIILVTGGGGSIGSEL
CRQIARFYPKQLLILDIYENNVYDVQMELNYNHPEVNKKVIIASIRDDDRLNEIFSHYKP
DVVFHAAAHKHVPLMEMNPAEAVKNNIIGTYNVLKCCNDYNVKKFVQISTDKAVNPTNIM
GATKRFCEMMVQAFDAVSKTEYVAVRFGNVLGSNGSVIPLFKKQISHGGPVTVTDPKINR
FFMTIPEAAQLVIQAGAIAHGGEIFVLDMGKPVKIVDLARDLITLSGYRPDEDIKIEYTG
LRPGEKLYEELLMNELALTSTEHKKIFVEKPNKIDMKFIEESILEFKEVVYEDREHIFKL
MEEKVPTYTRKNNERESA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory