SitesBLAST

P30901 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus helveticus (Lactobacillus suntoryeus) (see paper)
34% identity, 95% coverage: 2:313/330 of query aligns to 3:316/337 of P30901

query
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P30901

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D176 (= D174) binding NAD(+)
VP 207:208 (≠ CP 204:205) binding NAD(+)
N213 (≠ T210) binding NAD(+)
D260 (= D257) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P26297 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) (see 2 papers)
35% identity, 95% coverage: 2:313/330 of query aligns to 3:316/333 of P26297

query
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P26297

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HI 156:157 (= HI 154:155) binding NAD(+)
D176 (= D174) binding NAD(+)
H206 (= H203) mutation to Q: Increase of activity.
VP 207:208 (≠ CP 204:205) binding NAD(+)
N213 (≠ T210) binding NAD(+)
R236 (= R233) mutation to K: Decrease of activity.
D260 (= D257) binding NAD(+); mutation to N: Decrease of activity.
E265 (= E262) mutation to Q: Decrease of activity.
H297 (= H294) mutation to Q: 90% loss of activity.

3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
39% identity, 83% coverage: 46:320/330 of query aligns to 44:320/334 of 3kb6B

query
sites
3kb6B

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active site: S97 (= S101), R231 (= R233), D255 (= D257), E260 (= E262), H294 (= H294)
binding lactic acid: F49 (= F51), S72 (= S76), V73 (≠ A77), G74 (= G78), Y96 (= Y100), R231 (= R233), H294 (= H294)
binding nicotinamide-adenine-dinucleotide: V73 (≠ A77), Y96 (= Y100), V101 (= V105), G150 (= G153), R151 (≠ H154), I152 (= I155), D171 (= D174), V172 (≠ P175), P203 (= P205), T229 (= T231), A230 (≠ G232), R231 (= R233), H294 (= H294), A296 (= A296), Y297 (≠ F297)

1j49A Insights into domain closure, substrate specificity and catalysis of d-lactate dehydrogenase from lactobacillus bulgaricus (see paper)
34% identity, 95% coverage: 2:313/330 of query aligns to 3:316/332 of 1j49A

query
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1j49A

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T

A

T

E

H

A

A

L

V

T

R

N

N

I

M

A

V

E

V

T

K

T

A

L

F

A

D

N

N

active site: S103 (= S101), R236 (= R233), D260 (= D257), E265 (= E262), H297 (= H294)
binding nicotinamide-adenine-dinucleotide: Y102 (= Y100), I107 (≠ V105), G153 (= G151), G155 (= G153), I157 (= I155), Y175 (≠ S173), D176 (= D174), I177 (≠ P175), V207 (≠ C204), P208 (= P205), N213 (≠ T210), V234 (≠ T231), S235 (≠ G232), R236 (= R233), H297 (= H294), A299 (= A296), F300 (= F297)

P17584 D-2-hydroxyisocaproate dehydrogenase; D-HICDH; EC 1.1.1.- from Lacticaseibacillus paracasei (Lactobacillus paracasei) (see paper)
32% identity, 100% coverage: 1:330/330 of query aligns to 1:331/333 of P17584

query
sites
P17584

M

M

R

K

I

I

A

I

V

A

F

Y

S

G

A

A

K

R

P

V

Y

D

D

E

R

I

T

Q

F

Y

L

F

T

K

R

Q

A

W

N

A

S

K

G

D

Q

T

R

G

H

N

H

T

L

L

S

E

F

Y

F

H

E

T

A

E

R

F

L

L

T

D

E

E

D

N

T

T

A

V

T

E

L

W

A

A

N

K

G

G

F

F

D

D

A

G

I

I

C

N

A

S

F

L

V

Q

N

T

D

T

C

P

L

Y

D

A

A

A

A

G

V

V

L

F

E

E

Q

K

L

M

H

H

N

A

G

Y

G

G

T

I

R

K

L

F

V

L

A

T

L

I

R

R

S

N

A

V

G

G

F

T

N

D

H

N

V

I

D

D

L

M

A

T

A

A

A

M

D

K

R

Q

L

Y

G

G

I

I

T

R

V

L

V

S

R

N

V

V

P

P

A

A

Y

Y

S

S

P

P

H

A

A

A

V

I

A

A

E

E

H

F

A

A

V

L

A

T

L

D

V

T

L

L

S

Y

L

L

N

L

R

R

M

N

T

M

Y

G

R

K

A

V

Y

Q

N

A

R

Q

V

L

R

Q

E

A

G

G

N

D

F

Y

A

E

L

K

D

A

G

G

-

T

L

F

L

I

G

G

F

K

D

E

L

L

H

G

G

Q

K

Q

T

T

V

V

G

G

I

V

I

M

G

G

T

T

G

G

H
|
H

I
|
I

G

G

L

Q

I

V

F

A

A

I

D

K

I

L

M

F

H

K

G

G

F

F

G

G

C

A

R

K

V

V

V

I

A

A

S

Y

D
|
D

P

P

F

Y

P

P

S

M

P

K

D

G

A

D

K

H

S

P

F

D

V

F

E

D

Y

Y

V

V

P

S

L

L

A

E

T

D

L

L

F

F

A

K

S

Q

A

S

D

D

I

V

I

I

S

D

L

L

H

H

C
x
V

P

P

L

G

T

I

P

E

E

Q

T
x
N

H

T

H

H

L

I

I

I

D

N

A

E

D

A

A

A

I

F

A

N

Q

L

M

M

K

K

E

P

G

G

V

A

M

I

L

V

I

I

N

N

T
|
T

G
x
A

R
|
R

G

P

R

N

V

L

V

I

D

D

T

T

Q

Q

V

A

V

M

I

L

A

S

G

N

L

L

K

K

S

S

G

G

K

K

I

L

G

A

R

G

L

V

G

G

L

I

D
|
D

V

T

Y

Y

E

E

E

Y

E

E

E

T

Q

E

L

D

F

L

E

N

D

L

L

A

S

K

Q

H

G

G

V

S

I

F

N

K

D

D

D

P

Q

L

F

W

M

D

R

E

L

L

T

L

T

G

F

M

H

P

N

N

V

V

L

V

I

L

T

S

G

P

H

H

Q

I

A

A

F

Y

T

T

A

E

E

T

A

A

L

V

T

H

N

N

I

M

A

V

E

Y

T

F

T

S

L

L

A

Q

N

H

I

L

D

V

A

D

F

F

E

L

R

T

G

K

S

G

G

E

T

T

L

S

H

T

R

E

V

V

T

T

T

G

P

P

HI 155:156 (= HI 154:155) binding NAD(+)
D175 (= D174) binding NAD(+)
V205 (≠ C204) binding NAD(+)
N211 (≠ T210) binding NAD(+)
TAR 232:234 (≠ TGR 231:233) binding NAD(+)
D258 (= D257) binding NAD(+)

1dxyA Structure of d-2-hydroxyisocaproate dehydrogenase (see paper)
32% identity, 99% coverage: 1:328/330 of query aligns to 1:329/330 of 1dxyA

query
sites
1dxyA

M

M

R

K

I

I

A

I

V

A

F

Y

S

G

A

A

K

R

P

V

Y

D

D

E

R

I

T

Q

F

Y

L

F

T

K

R

Q

A

W

N

A

S

K

G

D

Q

T

R

G

H

N

H

T

L

L

S

E

F

Y

F

H

E

T

A

E

R

F

L

L

T

D

E

E

D

N

T

T

A

V

T

E

L

W

A

A

N

K

G

G

F

F

D

D

A

G

I

I

C

N

A

S

F

L

V

Q

N

T

D

T

C

P

L

Y

D

A

A

A

A

G

V

V

L

F

E

E

Q

K

L

M

H

H

N

A

G

Y

G

G

T

I

R

K

L

F

V

L

A

T

L

I

R

R

S

N

A
x
V

G

G

F

T

N

D

H

N

V

I

D

D

L

M

A

T

A

A

A

M

D

K

R

Q

L

Y

G

G

I

I

T

R

V

L

V

S

R

N

V

V

P

P

A

A

Y
|
Y

S
|
S

P

P

H

A

A

A

V

I

A

A

E

E

H

F

A

A

V

L

A

T

L

D

V

T

L

L

S

Y

L

L

N

L

R

R

M

N

T

M

Y

G

R

K

A

V

Y

Q

N

A

R

Q

V

L

R

Q

E

A

G

G

N

D

F

Y

A

E

L

K

D

A

G

G

-

T

L

F

L

I

G

G

F

K

D

E

L

L

H

G

G

Q

K

Q

T

T

V

V

G

G

I

V

I

M

G
|
G

T

T

G
|
G

H
|
H

I
|
I

G

G

L

Q

I

V

F

A

A

I

D

K

I

L

M

F

H

K

G

G

F

F

G

G

C

A

R

K

V

V

V

I

A

A

S
x
Y

D
|
D

P
|
P

F

Y

P

P

S

M

P

K

D

G

A

D

K

H

S

P

F

D

V

F

E

D

Y

Y

V

V

P

S

L

L

A

E

T

D

L

L

F

F

A

K

S

Q

A

S

D

D

I

V

I

I

S

D

L

L

H
|
H

C
x
V

P
|
P

L

G

T

I

P

E

E

Q

T
x
N

H

T

H

H

L

I

I

I

D

N

A

E

D

A

A

A

I

F

A

N

Q

L

M

M

K

K

E

P

G

G

V

A

M

I

L

V

I

I

N

N

T
|
T

G
x
A

R
|
R

G

P

R

N

V

L

V

I

D

D

T

T

Q

Q

V

A

V

M

I

L

A

S

G

N

L

L

K

K

S

S

G

G

K

K

I

L

G

A

R

G

L

V

G

G

L

I

D
|
D

V

T

Y

Y

E

E

E

Y

E
|
E

E

T

Q

E

L

D

F

L

E

N

D

L

L

A

S

K

Q

H

G

G

V

S

I

F

N

K

D

D

D

P

Q

L

F

W

M

D

R

E

L

L

T

L

T

G

F

M

H

P

N

N

V

V

L

V

I

L

T

S

G

P

H
|
H

Q

I

A

A

F
x
Y

F

Y

T

T

A

E

E

T

A

A

L

V

T

H

N

N

I

M

A

V

E

Y

T

F

T

S

L

L

A

Q

N

H

I

L

D

V

A

D

F

F

E

L

R

T

G

K

S

G

G

E

T

T

L

S

H

T

R

E

V

V

T

T

active site: S101 (= S101), R234 (= R233), D258 (= D257), E263 (= E262), H295 (= H294)
binding 2-oxo-4-methylpentanoic acid: V77 (≠ A77), Y100 (= Y100), Y298 (≠ F297)
binding nicotinamide-adenine-dinucleotide: Y100 (= Y100), G152 (= G151), G154 (= G153), H155 (= H154), I156 (= I155), Y174 (≠ S173), D175 (= D174), P176 (= P175), H204 (= H203), V205 (≠ C204), P206 (= P205), N211 (≠ T210), T232 (= T231), A233 (≠ G232), R234 (= R233), H295 (= H294), Y298 (≠ F297)

4prlA Crystal structure of d-lactate dehydrogenase with NAD+ from lactobacillus jensenii (see paper)
32% identity, 97% coverage: 1:320/330 of query aligns to 1:321/330 of 4prlA

query
sites
4prlA

M

M

R

K

I

I

A

A

V

V

F

F

S

S

A

P

K

S

P

E

Y

S

D

E

R

R

T

K

F

L

L

V

T

A

R

A

A

T

N

E

S

K

G

K

Q

F

R

G

H

C

H

E

L

L

S

K

F

L

F

I

E

D

A

E

R

S

L

L

T

S

-

A

E

E

D

N

T

V

A

D

T

Q

L

V

A

A

N

D

G

C

F

D

D

G

A

V

I

L

C

L

A

K

F

P

V

L

N

G

D

N

C

L

L

D

D

D

A

E

A

I

V

V

L

Y

E

K

Q

K

L

L

H

A

N

D

G

Y

G

G

T

I

R

K

L

S

V

I

A

G

L

L

R

R

S

I

A

V

G

G

F

T

N

N

H

T

V

I

D

D

L

F

A

D

A

L

A

A

D

K

R

K

L

Y

G

H

I

L

T

T

V

V

V

T

R

N

V

V

P

P

A

V

Y
|
Y

S

S

P

P

H

R

A

A

V
x
I

A

A

E

E

H

M

A

A

V

V

A

T

L

Q

V

A

L

M

S

Y

L

L

N

N

R

R

M

K

T

I

Y

G

R

E

A

F

Y

K

N

A

R

N

V

M

R

D

E

K

G

G

N

D

F

F

A

T

-

N

L

P

D

D

G

S

L

L

L

I

G

S

F

N

D

E

L

I

H

Y

G

N

K

K

T

T

V

I

G

G

I

L

I

I

G

G

T
x
V

G
|
G

H
|
H

I
|
I

G

G

L

S

I

A

F

V

A

A

D

Q

I

I

M

F

H

S

G

A

F

M

G

G

C

A

R

K

V

V

V

L

A

A

S
x
Y

D
|
D

P

V

F

I

P

Y

S

N

P

P

D

E

A

V

K

E

S

P

F

Y

V

L

E

T

Y

Y

V

A

P

D

L

F

A

D

T

T

L

V

F

L

A

K

S

E

A

A

D

D

I

I

S

S

L

L

H
|
H

C
x
T

P
|
P

L

L

T

L

P

K

E

S

T

T

H

E

H

N

L

M

I

I

D

G

A

K

D

K

A

Q

I

F

A

A

Q

E

M

M

K

K

E

N

G

D

V

A

M

I

L

L

I

I

N

N

T
x
A

G
x
A

R

R

G

G

R

E

V

L

V

V

D

D

T

T

Q

A

V

A

V

L

I

I

A

E

G

A

L

L

K

E

S

K

G

H

K

E

I

I

G

A

R

A

L

A

G

G

L

L

D
|
D

V

T

Y

L

E

A

E

H

E

E

E

S

Q

S

L

Y

F

F

E

K

D

K

L

V

S

D

Q

D

G

A

V

Q

I

I

N

P

D

A

D

D

Q

-

F

Y

M

K

R

K

L

L

T

A

F

M

H

P

N

N

V

V

L

I

I

V

T

T

G

P

H
|
H

Q

S

A
|
A

F

Y

F

F

T

T

A

K

E

T

A

S

L

V

T

R

N

N

I

M

A

I

E

E

T

I

T

S

L

L

A

R

N

D

I

T

D

I

A

A

F

L

E

A

R

N

G

G

binding nicotinamide-adenine-dinucleotide: Y101 (= Y100), I106 (≠ V105), V154 (≠ T152), G155 (= G153), H156 (= H154), I157 (= I155), Y175 (≠ S173), D176 (= D174), H205 (= H203), T206 (≠ C204), P207 (= P205), A233 (≠ T231), A234 (≠ G232), D259 (= D257), H295 (= H294), A297 (= A296)

4xkjA A novel d-lactate dehydrogenase from sporolactobacillus sp
35% identity, 99% coverage: 1:327/330 of query aligns to 1:328/332 of 4xkjA

query
sites
4xkjA

M

M

R

K

I

I

A

I

V

M

F

F

S

S

A

V

K

R

P

D

Y

D

D

E

R

E

T

A

F

A

L

I

T

R

R

E

A

W

N

E

S

K

G

K

Q

T

R

G

H

V

H

Q

L

V

S

D

F

I

F

N

E

R

A

L

R

E

L

L

T

D

E

A

D

E

T

T

A

A

T

Q

L

L

A

T

N

K

G

G

F

Y

D

D

A

G

I

I

C

V

A

I

F

Q

V

Q

N

R

D

S

C

H

L

I

-

S

D

N

A

P

A

A

V

V

L

Y

E

E

Q

T

L

L

H

Q

N

K

G

N

G

G

T

L

R

R

L

Q

V

L

A

T

L

S

R

R

S

T

A

A

G

G

F

Y

N

D

H

M

V

I

D

D

L

L

A

E

A

Q

A

A

D

S

R

E

L

R

G

G

I

L

T

V

V

V

V

T

R

N

V

V

P

P

A

A

Y
|
Y

S
|
S

P

P

H

N

A

S

V
|
V

A

A

E

E

H

L

A

A

V

L

A

T

L

Q

V

T

L

M

S

R

L

L

N

I

R

R

M

N

T

L

Y

P

R

L

A

F

Y

D

N

A

R

R

V

G

R

A

E

E

G

Q

N

D

F

F

A

R

L

W

D

A

G

G

L

L

L

M

G

A

F

R

D

E

L

I

H

R

G

S

K

L

T

T

V

V

G

G

I

I

G
|
G

T

A

G
|
G

H
x
R

I
|
I

G

G

L

G

I

T

F

V

A

A

D

R

I

L

M

F

H

K

G

A

F

L

G

G

C

A

R

T