SitesBLAST
Comparing WP_039969720.1 NCBI__GCF_000195635.1:WP_039969720.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P32171 L-Rhamnulokinase; RhaB; RhuK; ATP:L-rhamnulose phosphotransferase; L-rhamnulose 1-kinase; Rhamnulose kinase; EC 2.7.1.5 from Escherichia coli (strain K12) (see paper)
42% identity, 96% coverage: 7:473/489 of query aligns to 8:471/489 of P32171
- ASSGR 13:17 (≠ ATSGR 12:16) binding ATP
- C68 (≠ A65) modified: Disulfide link with 222
- E69 (≠ R66) mutation to A: Same kinase activity as the wild-type; when associated with A-70 and A-73.
- E70 (= E68) mutation to A: Same kinase activity as the wild-type; when associated with A-69 and A-73.
- R73 (≠ E71) mutation to A: Same kinase activity as the wild-type; when associated with A-69 and A-70.
- G83 (= G81) binding substrate
- C222 (≠ T219) modified: Disulfide link with 68
- HDT 236:238 (= HDT 239:241) binding substrate
- D237 (= D240) active site, Proton acceptor
- T259 (= T262) binding ATP
- N296 (= N299) binding substrate
- Q304 (≠ E307) binding ATP
- C353 (= C356) modified: Disulfide link with 370
- C370 (= C373) modified: Disulfide link with 353
- G402 (= G405) binding ATP
2uytA Structure of l-rhamnulose kinase in complex with adp and beta-l- rhamnulose. (see paper)
42% identity, 96% coverage: 7:473/489 of query aligns to 7:470/479 of 2uytA
- binding adenosine-5'-diphosphate: S13 (≠ T13), R16 (= R16), G257 (= G261), T258 (= T262), Q303 (≠ E307), L314 (≠ Y318), G401 (= G405), Q404 (≠ R408), N405 (= N409)
- binding 6-deoxy-beta-L-fructofuranose: W81 (= W80), H235 (= H239), D236 (= D240), W259 (= W263)
2cglA Crystal structure of l-rhamnulose kinase from escherichia coli in complex with l-fructose, adp and a modeled atp gamma phosphate. (see paper)
42% identity, 96% coverage: 7:473/489 of query aligns to 7:470/479 of 2cglA
- binding adenosine-5'-diphosphate: S13 (≠ T13), R16 (= R16), G257 (= G261), T258 (= T262), Q303 (≠ E307), L314 (≠ Y318), G401 (= G405), Q404 (≠ R408), N405 (= N409)
- binding beta-L-fructofuranose: W81 (= W80), G82 (= G81), H235 (= H239), D236 (= D240), W259 (= W263), N295 (= N299)
- binding phosphite ion: G11 (= G11), T258 (= T262)
2cgjA Crystal structure of l-rhamnulose kinase from escherichia coli in complex with l-fructose and adp. (see paper)
42% identity, 96% coverage: 7:473/489 of query aligns to 7:470/479 of 2cgjA
- binding adenosine-5'-diphosphate: S13 (≠ T13), G257 (= G261), T258 (= T262), Q303 (≠ E307), L314 (≠ Y318), G401 (= G405), Q404 (≠ R408), N405 (= N409)
- binding beta-L-fructofuranose: G82 (= G81), Y101 (= Y100), H235 (= H239), D236 (= D240), T237 (= T241), W259 (= W263), N295 (= N299)
Q1R415 Rhamnulokinase; Rhamnulose kinase; EC 2.7.1.5 from Escherichia coli (strain UTI89 / UPEC) (see paper)
41% identity, 96% coverage: 7:473/489 of query aligns to 8:471/489 of Q1R415
- ASSGR 13:17 (≠ ATSGR 12:16) binding ATP
- G83 (= G81) binding substrate
- HDT 236:238 (= HDT 239:241) binding substrate
- D237 (= D240) active site, Proton acceptor
- T259 (= T262) binding ATP
- N296 (= N299) binding substrate
- Q304 (≠ E307) binding ATP
- G402 (= G405) binding ATP
P18157 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Bacillus subtilis (strain 168) (see paper)
22% identity, 96% coverage: 1:469/489 of query aligns to 1:472/496 of P18157
- H230 (≠ Q223) mutation to R: Increased activity.
- F232 (≠ M225) mutation to S: Increased activity.
3h3nX Glycerol kinase h232r with glycerol (see paper)
24% identity, 99% coverage: 2:486/489 of query aligns to 3:491/501 of 3h3nX
3ll3B The crystal structure of ligand bound xylulose kinase from lactobacillus acidophilus
20% identity, 96% coverage: 5:472/489 of query aligns to 4:464/490 of 3ll3B
- binding adenosine-5'-diphosphate: G258 (= G261), T259 (= T262), G298 (≠ R295), P314 (≠ S317), G399 (= G405), F400 (≠ G406), K402 (≠ R408)
- binding 1-deoxy-d-xylulose-5-phosphate: H127 (≠ M130), N295 (= N292), G338 (≠ D347), E340 (= E349), A347 (≠ S357)
O34153 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus casseliflavus (Enterococcus flavescens) (see 3 papers)
24% identity, 99% coverage: 2:486/489 of query aligns to 4:492/506 of O34153
- R84 (≠ G81) binding glycerol
- E85 (≠ V82) binding glycerol
- Y136 (≠ F132) binding glycerol
- H232 (≠ T226) modified: Phosphohistidine; by HPr; mutation to A: Loss of phosphorylation, no effect on activity.; mutation to E: Loss of phosphorylation, 2.5-fold reduced activity.; mutation to R: Loss of phosphorylation, 3.4-fold increased activity.
- D246 (= D240) binding glycerol
- Q247 (≠ T241) binding glycerol
3ll3A The crystal structure of ligand bound xylulose kinase from lactobacillus acidophilus
20% identity, 96% coverage: 5:472/489 of query aligns to 5:466/492 of 3ll3A