SitesBLAST
Comparing WP_040685231.1 NCBI__GCF_000341205.1:WP_040685231.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
36% identity, 90% coverage: 24:306/315 of query aligns to 22:304/304 of 1wwkA
- active site: S96 (≠ I99), R230 (= R232), D254 (= D256), E259 (= E261), H278 (= H280)
- binding nicotinamide-adenine-dinucleotide: V100 (≠ T102), G146 (= G147), F147 (≠ L148), G148 (= G149), R149 (= R150), I150 (= I151), Y168 (≠ W169), D169 (≠ S170), P170 (≠ E171), V201 (≠ L203), P202 (≠ Q204), T207 (= T209), T228 (= T230), S229 (= S231), D254 (= D256), H278 (= H280), G280 (= G282)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
34% identity, 87% coverage: 35:307/315 of query aligns to 37:315/334 of 5aovA
- active site: L100 (≠ I99), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H280)
- binding glyoxylic acid: M52 (= M50), L53 (vs. gap), L53 (vs. gap), Y74 (≠ T73), A75 (≠ G74), V76 (≠ R75), G77 (≠ R76), R241 (= R232), H288 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ R75), T104 (= T102), F158 (≠ L148), G159 (= G149), R160 (= R150), I161 (= I151), S180 (= S170), R181 (≠ E171), A211 (≠ H202), V212 (≠ L203), P213 (≠ Q204), T218 (= T209), I239 (≠ T230), A240 (≠ S231), R241 (= R232), H288 (= H280), G290 (= G282)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 20:281/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G149), I148 (= I151), Y166 (≠ W169), D167 (≠ S170), P168 (≠ E171), I169 (≠ N172), I170 (≠ L173), H198 (= H202), T199 (≠ L203), L208 (= L212), R228 (= R232)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 23:284/303 of 6plgA
7dkmA Phgdh covalently linked to oridonin (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 24:285/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ R75), A102 (≠ T102), G148 (= G147), R151 (= R150), I152 (= I151), Y170 (≠ W169), D171 (≠ S170), P172 (≠ E171), I173 (≠ N172), H202 (= H202), T203 (≠ L203), P204 (≠ Q204), T209 (= T209), C230 (≠ T230), A231 (≠ S231), R232 (= R232), H279 (= H280), G281 (= G282)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 23:284/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (= L146), G147 (= G147), L148 (= L148), G149 (= G149), R150 (= R150), I151 (= I151), G152 (= G152), D170 (≠ S170), H201 (= H202), T202 (≠ L203), P203 (≠ Q204)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 23:284/302 of 6rihA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 24:285/305 of 6plfA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 22:283/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ I99), A100 (≠ T102), R149 (= R150), I150 (= I151), Y168 (≠ W169), D169 (≠ S170), P170 (≠ E171), I171 (≠ N172), H200 (= H202), T201 (≠ L203), P202 (≠ Q204), T207 (= T209), C228 (≠ T230), A229 (≠ S231), R230 (= R232), H277 (= H280), G279 (= G282)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 22:283/297 of 6rj3A
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
36% identity, 83% coverage: 24:286/315 of query aligns to 23:284/301 of 6rj5A
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
36% identity, 84% coverage: 24:287/315 of query aligns to 28:290/533 of O43175
- T78 (≠ R75) binding NAD(+)
- R135 (vs. gap) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 150:151) binding NAD(+)
- D175 (≠ S170) binding NAD(+)
- T207 (≠ L203) binding NAD(+)
- CAR 234:236 (≠ TSR 230:232) binding NAD(+)
- D260 (= D256) binding NAD(+)
- V261 (= V257) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HLGY 280:283) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
37% identity, 93% coverage: 18:309/315 of query aligns to 19:307/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
37% identity, 93% coverage: 18:309/315 of query aligns to 18:306/526 of 3dc2A
Sites not aligning to the query:
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
34% identity, 81% coverage: 60:313/315 of query aligns to 58:327/334 of 3kb6B
- active site: S97 (= S100), R231 (= R232), D255 (= D256), E260 (= E261), H294 (= H280)
- binding lactic acid: S72 (≠ G74), V73 (≠ R75), G74 (≠ R76), Y96 (≠ I99), R231 (= R232), H294 (= H280)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ R75), Y96 (≠ I99), V101 (≠ T102), G150 (= G149), R151 (= R150), I152 (= I151), D171 (≠ E171), V172 (≠ N172), P203 (≠ Q204), T229 (= T230), A230 (≠ S231), R231 (= R232), H294 (= H280), A296 (≠ G282), Y297 (= Y283)
Sites not aligning to the query:
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
33% identity, 86% coverage: 37:307/315 of query aligns to 38:314/332 of 6biiA