SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_041091094.1 NCBI__GCF_000829435.1:WP_041091094.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 87% coverage: 16:253/273 of query aligns to 26:263/265 of P07821

query
sites
P07821

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K50 (= K40) mutation to Q: Lack of activity.
D172 (= D162) mutation to E: Lack of activity.
E173 (= E163) mutation to A: Lack of activity.

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
35% identity, 81% coverage: 1:221/273 of query aligns to 1:228/232 of 1f3oA

query
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1f3oA

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binding adenosine-5'-diphosphate: Y11 (= Y11), G41 (= G37), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
31% identity, 83% coverage: 1:226/273 of query aligns to 1:224/240 of 4ymuJ

query
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4ymuJ

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binding adenosine-5'-triphosphate: F11 (≠ Y11), V16 (≠ I16), S36 (≠ N36), G37 (= G37), S38 (≠ C38), G39 (= G39), K40 (= K40), S41 (= S41), T42 (= T42), E162 (= E163), H194 (= H196)
binding magnesium ion: S41 (= S41), E162 (= E163)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
35% identity, 81% coverage: 1:221/273 of query aligns to 1:228/230 of 1l2tA

query
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1l2tA

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binding adenosine-5'-triphosphate: Y11 (= Y11), S40 (≠ N36), G41 (= G37), S42 (≠ C38), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42), F138 (= F134), Q145 (≠ E137), S147 (= S139), G149 (= G141), Q150 (= Q142), H204 (= H196)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
30% identity, 79% coverage: 12:227/273 of query aligns to 37:252/382 of 7ahhC

query
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7ahhC

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binding phosphoaminophosphonic acid-adenylate ester: T39 (≠ S14), V40 (≠ I15), G41 (≠ I16), G62 (= G37), G64 (= G39), K65 (= K40), D187 (= D162), E188 (= E163)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
30% identity, 79% coverage: 12:227/273 of query aligns to 37:252/382 of 7aheC

query
sites
7aheC

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L

L

L

I

K

E

P

P

Q

T

S

S

G

G

S

K

V

I

Q

F

L

I

N

D

G

N

K

Q

E

D

I

V

S

A

-

T

-

L

-

N

-

K

-

E

-

D

-

L

-

Q

-

V

-

R

R

R

Y

K

S

T

T

M

R

S

K

M

V

V

A

F

Q

Q

E

N

L

F

A

G

I

L

L

F

P

P

Q

H

S

R

P

-

T

-

A

-

P

-

E

-

G

-

L

-

T

T

V

I

L

L

Q

E

L

N

V

T

K

E

Q

Y

G

G

R

L

Y

E

P

V

Y

Q

Q

N

G

V

F

P

M

K

H

E

Q

E

W

R

S

R

K

K

E

R

D

A

E

E

E

K

C

A

V

L

Q

D

N

N

A

A

L

-

K

-

A

-

T

-

G

N

M

L

E

L

E

D

L

F

Q

K

D

D

R

Q

F

Y

V

P

D

K

E

Q

L

L

S

S

G

G

Q

M

R

Q

Q

Q

R

R

A

V

W

G

I

L

A

A

M

R

T

A

L

L

A

A

Q

N

D

D

T

P

D

E

I

I

I

L

L

L

L

M

D

D

E

E

P

A

T

F

T

S

Y

A

L

L

D

D

M

P

T

L

H

I

Q

R

I

R

E

E

I

M

L

Q

D

D

L

E

L

L

F

L

D

E

L

L

N

Q

E

A

N

K

E

F

N

Q

R

K

T

T

I

I

V

I

M

F

V

V

L

S

H

H

D

D

L

L

N

N

L

E

A

A

C

L

R

R

Y

I

A

G

H

D

N

R

L

I

I

A

A

I

V

M

K

K

D

D

R

G

K

K

V

I

Y

M

Q

Q

Q

I

G

G

K

T

P

G

E

E

V

I

I

L

S

T

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
30% identity, 79% coverage: 12:227/273 of query aligns to 37:252/260 of 7ahdC

query
sites
7ahdC

G

G

D

A

S
x
T

I

V

I

G

I

V

E

Y

E

D

L

T

N

N

L

F

T

E

I

I

P

N

K

E

G

G

E

E

I

I

S

F

V

V

L

I

I

M

G

G

G

L

N
x
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

S

L

M

L

A

N

R

R

L

L

L

I

K

E

P

P

Q

T

S

S

G

G

S

K

V

I

Q

F

L

I

N

D

G

N

K

Q

E

D

I

V

S

A

-

T

-

L

-

N

-

K

-

E

-

D

-

L

-

Q

-

V

-

R

R

R

Y

K

S

T

T

M

R

S

K

M

V

V

A

F

Q
|
Q

E

N

L

F

A

G

I

L

L

F

P

P

Q

H

S

R

P

-

T

-

A

-

P

-

E

-

G

-

L

-

T

T

V

I

L

L

Q

E

L

N

V

T

K

E

Q

Y

G

G

R

L

Y

E

P

V

Y

Q

Q

N

G

V

F

P

M

K

H

E

Q

E

W

R

S

R

K

K

E

R

D

A

E

E

E

K

C

A

V

L

Q

D

N

N

A

A

L

-

K

-

A

-

T

-

G

N

M

L

E

L

E

D

L

F

Q

K

D

D

R

Q

F

Y

V

P

D
x
K

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
x
M

R

Q

Q

Q

R

R

A

V

W

G

I

L

A

A

M

R

T

A

L

L

A

A

Q

N

D

D

T

P

D

E

I

I

I

L

L

L

L

M

D

D

E
x
Q

P

A

T

F

T

S

Y

A

L

L

D

D

M

P

T

L

H

I

Q

R

I

R

E

E

I

M

L

Q

D

D

L

E

L

L

F

L

D

E

L

L

N

Q

E

A

N

K

E

F

N

Q

R

K

T

T

I

I

V

I

M

F

V

V

L

S

H
|
H

D

D

L

L

N

N

L

E

A

A

C

L

R

R

Y

I

A

G

H

D

N

R

L

I

I

A

A

I

V

M

K

K

D

D

R

G

K

K

V

I

Y

M

Q

Q

Q

I

G

G

K

T

P

G

E

E

V

I

I

L

S

T

binding adenosine-5'-triphosphate: T39 (≠ S14), S61 (≠ N36), G62 (= G37), G64 (= G39), K65 (= K40), S66 (= S41), T67 (= T42), Q111 (= Q75), K161 (≠ D136), Q162 (≠ E137), S164 (= S139), G166 (= G141), M167 (≠ Q142), Q188 (≠ E163), H221 (= H196)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8g4cB Bceabs atpgs high res tm (see paper)
30% identity, 80% coverage: 1:219/273 of query aligns to 3:224/248 of 8g4cB

query
sites
8g4cB

M

I

L

L

K

E

T

A

Q

N

S

K

L

I

T

R

L

K

S

S

Y
|
Y

G

G

D

N

S

K

I

L

-

N

-

K

-

Q

-

E

I

V

I

L

E

K

E

G

L

I

N

D

L

I

T

H

I

I

P

E

K

K

G

G

E

E

I

F

S

V

V

S

L

I

I

M

G

G

G

A

N
x
S

G

G

C
x
S

G

G

K

K

S
x
T

T

T

L

L

R

N

S

V

M

L

A

S

R

S

L

I

L

D

K

Q

P

V

Q

S

S

H

G

G

S

T

V

I

Q

H

L

I

N

N

G

G

K

N

E

D

I

M

S

T

R

A

Y

M

S

K

T

E

R

K

K

Q

V

L

A

A

-

E

-

F

-

R

-

K

Q

Q

E

H

L

L

A

G

I

F

L

I

P

F

Q

Q

S

D

P

Y

T

N

A

L

P

L

E

D

G

T

L

L

T

T

V

V

L

-

Q

-

L

-

V

-

K

K

Q

E

G

N

R

I

Y

L

P

L

Y

P

Q

L

G

S

F

I

M

T

H

K

Q

L

W

S

S

K

K

K

E

E

D

A

E

N

E

R

C

K

V

F

Q

E

N

E

A

V

L

A

K

K

A

E

T

L

G

G

M

I

E

Y

E

E

L

L

Q

R

D

D

R

K

F

Y

V

P

D

N

E

E

L

I

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

T

W

S

I

A

A

G

M

R

T

A

L

F

A

I

Q

H

D

D

T

P

D

S

I

I

L

F

L

A

D
|
D

E

E

P

P

T

T

T

G

Y

A

L

L

D

D

M

S

T

K

H

S

Q

A

I

S

E

D

I

L

L

L

D

N

L

K

L

L

F

S

D

Q

L

L

N

N

E

Q

N

K

E

R

N

N

R

A

T

T

I

I

V

I

M

M

V

V

L

T

H

H

D

D

L

-

N

P

L

V

A

A

C

A

R

S

Y

Y

A

C

H

G

N

R

L

V

I

I

A

F

V

I

K

K

D

D

R

G

K

Q

V

M

Y

Y

Q

T

Q

Q

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y11), S42 (≠ N36), S44 (≠ C38), T47 (≠ S41), D168 (= D162)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 83% coverage: 1:227/273 of query aligns to 2:225/241 of 4u00A

query
sites
4u00A

M

I

L

I

K

R

T

I

Q

R

S

N

L

L

T

H

L

K

S

W

Y
x
F

G

G

D

P

S

L

I

H

I
x
V

I

L

E

K

E

G

L

I

N

H

L

L

T

E

I

V

P

A

K

P

G

G

E

E

I

K

S

L

V

V

L

I

I

I

G

G

G

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

S

T

M

I

A

N

R

R

L

L

L

E

K

D

P

F

Q

Q

S

E

G

G

S

E

V

V

Q

V

L

V

N

D

G

G

K

L

E

S

I

V

S

K

R

D

-

D

Y

R

S

A

T

L

R

R

K

E

V

I

A

R

Q

R

E

E

L

V

A

G

I

M

L

V

P

F

Q

Q

S

Q

P

F

T

N

A

L

P

F

E

P

G

H

L

M

T

T

V

V

L

L

Q

E

L

N

V

V

K

T

Q

L

G

A

R

-

Y

-

P

P

Y

M

Q

R

G

-

F

-

M

V

H

R

Q

R

W

W

S

P

K

R

E

E

D

K

-

A

E

E

E

K

C

K

V

A

Q

L

N

E

A

L

L

L

K

E

A

R

T

V

G

G

M

I

E

L

E

D

L

Q

Q

A

D

R

R

K

F

Y

V

P

D

A

E

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

L

L

A

A

Q

M

D

E

T

P

D

K

I

I

I

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

Y

A

L

L

D

D

M

P

T

E

H

M

Q

V

I

G

E

E

I

V

L

L

D

D

L

V

L

M

F

R

D

D

L

L

N

A

E

Q

N

G

E

-

N

G

R

M

T

T

I

M

V

V

M

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

C

R

R

E

Y

V

A

A

H

D

N

R

L

V

I

V

A

F

V

M

K

D

D

G

R

G

K

Q

V

I

Y

V

Q

E

G

G

K

R

P

P

E

E

V

I

I

F

S

T

binding adenosine-5'-diphosphate: F12 (≠ Y11), V17 (≠ I16), S37 (≠ N36), G38 (= G37), S39 (≠ C38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (= T42)

7tchB Bceab e169q variant atp-bound conformation (see paper)
29% identity, 80% coverage: 1:219/273 of query aligns to 2:223/245 of 7tchB

query
sites
7tchB

M

I

L

L

K

E

T

A

Q

N

S

K

L

I

T

R

L

K

S

S

Y
|
Y

G

G

D

N

S

K

I

L

-

N

-

K

-
x
Q

-

E

I
x
V

I

L

E

K

E

G

L

I

N

D

L

I

T

H

I

I

P

E

K

K

G

G

E

E

I

F

S

V

V

S

L

I

I

M

G

G

G

A

N
x
S

G
|
G

C
x
S

G

G

K
|
K

S
x
T

T

T

L

L

R

N

S

V

M

L

A

S

R

S

L

I

L

D

K

Q

P

V

Q

S

S

H

G

G

S

T

V

I

Q

H

L

I

N

N

G

G

K

N

E

D

I

M

S

T

R

A

Y

M

S

K

T

E

R

K

K

Q

V

L

A

A

-

E

-

F

-

R

-

K

Q

Q

E

H

L

L

A

G

I

F

L

I

P

F

Q

Q

S

D

P

Y

T

N

A

L

P

L

E

D

G

T

L

L

T

T

V

V

L

-

Q

-

L

-

V

-

K

K

Q

E

G

N

R

I

Y

L

P

L

Y

P

Q

L

G

S

F

I

M

T

H

K

Q

L

W

S

S

K

K

K

E

E

D

A

E

N

E

R

C

K

V

F

Q

E

N

E

A

V

L

A

K

K

A

E

T

L

G

G

M

I

E

Y

E

E

L

L

Q

R

D

D

R

K

F

Y

V

P

D

N

E
|
E

L

I

S

S

G
|
G

Q

Q

R

K

Q

Q

R

R

A

T

W

S

I

A

A

G

M

R

T

A

L

F

A

I

Q

H

D

D

T

P

D

S

I

I

L

F

L

A

D

D

E

Q

P

P

T

T

T

G

Y

A

L

L

D

D

M

S

T

K

H

S

Q

A

I

S

E

D

I

L

L

L

D

N

L

K

L

L

F

S

D

Q

L

L

N

N

E

Q

N

K

E

R

N

N

R

A

T

T

I

I

V

I

M

M

V

V

L

T

H

H

D

D

L

-

N

P

L

V

A

A

C

A

R

S

Y

Y

A

C

H

G

N

R

L

V

I

I

A

F

V

I

K

K

D

D

R

G

K

Q

V

M

Y

Y

Q

T

Q

Q

binding adenosine-5'-triphosphate: Y12 (= Y11), Q19 (vs. gap), V21 (≠ I16), S41 (≠ N36), G42 (= G37), S43 (≠ C38), K45 (= K40), T46 (≠ S41), E142 (= E137), G145 (= G140), G146 (= G141)

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
34% identity, 82% coverage: 2:225/273 of query aligns to 4:225/350 of 3fvqB

query
sites
3fvqB

L

L

K

H

T

I

Q

G

S

H

L

L

T

S

L

K

S

S

Y
x
F

G
x
Q

D

N

S
x
T

I

P

I
x
V

I

L

E

N

E

D

L

I

N

S

L

L

T

S

I

L

P

D

K

P

G

G

E

E

I

I

S

L

V

F

L

I

I

I

G

G

G

A

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

L

L

R

R

S

C

M

L

A

A

R

G

L

F

L

E

K

Q

P

P

Q

D

S

S

G

G

S

E

V

I

Q

S

L

L

N

S

G

G

K

K

E

T

I

I

S

F

R

S

Y

K

S

N

T

T

R

N

K

L

V

P

A

V

Q

R

E

E

-

R

L

L

A

G

I

Y

L

L

P

V

Q
|
Q

S

E

P

G

T

V

A

L

P

F

E

P

G

H

L

L

T

T

V

V

L

Y

Q

R

L

N

V

I

K

A

Q

Y

G

G

R

L

Y

G

P

N

Y

G

Q

K

G

G

F

-

M

-

H

-

Q

-

W

R

S

T

K

A

E

Q

D

E

E

R

E

Q

C

R

V

I

Q

E

N

A

A

M

L

L

K

E

A

L

T

T

G

G

M

I

E

S

E

E

L

L

Q

A

D

G

R
|
R

F

Y

V

P

D

H

E
|
E

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

Q

Q

Q

R

R

A

A

W

A

I

L

A

A

M

R

T

A

L

L

A

A

Q

P

D

D

T

P

D

E

I

L

I

I

L

L

D

D

E

E

P

P

T

F

T

S

Y

A

L

L

D

D

M

E

T

Q

H

L

Q

R

I

R

E

Q

I

I

L

R

-

E

D

D

L

M

L

I

F

A

D

A

L

L

N

R

E

A

N

N

E

-

N

G

R

K

T

S

I

A

V

V

M

F

V

V

L

S

H

H

D

D

L

R

N

E

L

E

A

A

C

L

R

Q

Y

Y

A

A

H

D

N

R

L

I

I

A

A

V

V

M

K

K

D

Q

R

G

K

R

V

I

Y

L

Q

Q

Q

T

G

A

K

S

P

P

E

H

E

E

V

L

binding adenosine-5'-triphosphate: F13 (≠ Y11), Q14 (≠ G12), T16 (≠ S14), V18 (≠ I16), S38 (≠ N36), G39 (= G37), C40 (= C38), G41 (= G39), K42 (= K40), T43 (≠ S41), T44 (= T42), R133 (= R133), E137 (= E137), S139 (= S139), G141 (= G141), Q142 (= Q142)
binding calcium ion: T43 (≠ S41), Q86 (= Q82)

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 83% coverage: 1:226/273 of query aligns to 3:226/242 of 3c4jA

query
sites
3c4jA

M

M

L

I

K

D

T

V

Q

H

S

Q

L

L

T

K

L

K

S

S

Y
x
F

G

G

D

S

S

L

I

E

I
x
V

I

L

E

K

E

G

L

I

N

N

L

V

T

H

I

I

P

R

K

E

G

G

E

E

I

V

S

V

V

V

L

V

I

I

G

G

G

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

S

C

M

L

A

N

R

L

L

L

L

E

K

D

P

F

Q

D

S

E

G

G

S

E

V

I

Q

I

L

I

N

D

G

G

K

I

E

N

I

L

S

K

R

A

Y

K

S

D

T

T

R

N

-

L

-

N

K

K

V

V

A

R

Q

E

E

E

L

V

A

G

I

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

L

M

T

T

V

V

L

L

Q

N

L

N

V

I

K

T

Q

L

G

A

R

-

Y

-

P

P

Y

M

Q

K

G

-

F

-

M

V

H

R

Q

K

W

W

S

P

K

R

E

E

D

K

E

A

E

E

C

A

-

K

V

A

Q

M

N

E

A

L

L

L

K

D

A

K

T

V

G

G

M

L

E

K

E

D

L

K

Q

A

D

H

R

A

F

Y

V

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

L

L

A

A

Q

M

D

E

T

P

D

K

I

I

I

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

Y

A

L

L

D

D

M

P

T

E

H

M

Q

V

I

G

E

E

I

V

L

L

D

S

L

V

L

M

F

K

D

Q

L

L

N

-

E

A

N

N

E

E

N

G

R

M

T

T

I

M

V

V

M

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

C

R

R

E

Y

V

A

G

H

D

N

R

L

V

I

L

A

F

V

M

K

D

D

G

R

G

K

Y

V

I

Y

I

Q

E

G

G

K

K

P

P

E

E

E

D

V

L

I

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y11), V18 (≠ I16), S38 (≠ N36), G39 (= G37), S40 (≠ C38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 83% coverage: 1:226/273 of query aligns to 3:226/242 of 3c41J

query
sites
3c41J

M

M

L

I

K

D

T

V

Q

H

S

Q

L

L

T

K

L

K

S

S

Y
x
F

G

G

D

S

S

L

I

E

I
x
V

I

L

E

K

E

G

L

I

N

N

L

V

T

H

I

I

P

R

K

E

G

G

E

E

I

V

S

V

V

V

L

V

I

I

G

G

G

P

N
x
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

S

C

M

L

A

N

R

L

L

L

L

E

K

D

P

F

Q

D

S

E

G

G

S

E

V

I

Q

I

L

I

N

D

G

G

K

I

E

N

I

L

S

K

R

A

Y

K

S

D

T

T

R

N

-

L

-

N

K

K

V

V

A

R

Q

E

E

E

L

V

A

G

I

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

L

M

T

T

V

V

L

L

Q

N

L

N

V

I

K

T

Q

L

G

A

R

-

Y

-

P

P

Y

M

Q

K

G

-

F

-

M

V

H

R

Q

K

W

W

S

P

K

R

E

E

D

K

E

A

E

E

C

A

-

K

V

A

Q

M

N

E

A

L

L

L

K

D

A

K

T

V

G

G

M

L

E

K

E

D

L

K

Q

A

D

H

R

A

F

Y

V

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

L

L

A

A

Q

M

D

E

T

P

D

K

I

I

I

M

L

L

L

F

D
|
D

E

E

P

P

T

T

T

S

Y

A

L

L

D

D

M

P

T

E

H

M

Q

V

I

G

E

E

I

V

L

L

D

S

L

V

L

M

F

K

D

Q

L

L

N

-

E

A

N

N

E

E

N

G

R

M

T

T

I

M

V

V

M

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

C

R

R

E

Y

V

A

G

H

D

N

R

L

V

I

L

A

F

V

M

K

D

D

G

R

G

K

Y

V

I

Y

I

Q

E

G

G

K

K

P

P

E

E

E

D

V

L

I

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y11), V18 (≠ I16), S38 (≠ N36), G39 (= G37), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)
binding magnesium ion: S43 (= S41), D163 (= D162)

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 83% coverage: 1:226/273 of query aligns to 3:226/242 of 2olkA

query
sites
2olkA

M

M

L

I

K

D

T

V

Q

H

S

Q

L

L

T

K

L

K

S

S

Y
x
F

G

G

D

S

S

L

I

E

I
x
V

I

L

E

K

E

G

L

I

N

N

L

V

T

H

I

I

P

R

K

E

G

G

E

E

I

V

S

V

V

V

L

V

I

I

G

G

G

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

S

C

M

L

A

N

R

L

L

L

L

E

K

D

P

F

Q

D

S

E

G

G

S

E

V

I

Q

I

L

I

N

D

G

G

K

I

E

N

I

L

S

K

R

A

Y

K

S

D

T

T

R

N

-

L

-

N

K

K

V

V

A

R

Q

E

E

E

L

V

A

G

I

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

L

M

T

T

V

V

L

L

Q

N

L

N

V

I

K

T

Q

L

G

A

R

-

Y

-

P

P

Y

M

Q

K

G

-

F

-

M

V

H

R

Q

K

W

W

S

P

K

R

E

E

D

K

E

A

E

E

C

A

-

K

V

A

Q

M

N

E

A

L

L

L

K

D

A

K

T

V

G

G

M

L

E

K

E

D

L

K

Q

A

D

H

R

A

F

Y

V

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

L

L

A

A

Q

M

D

E

T

P

D

K

I

I

I

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

Y

A

L

L

D

D

M

P

T

E

H

M

Q

V

I

G

E

E

I

V

L

L

D

S

L

V

L

M

F

K

D

Q

L

L

N

-

E

A

N

N

E

E

N

G

R

M

T

T

I

M

V

V

M

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

C

R

R

E

Y

V

A

G

H

D

N

R

L

V

I

L

A

F

V

M

K

D

D

G

R

G

K

Y

V

I

Y

I

Q

E

G

G

K

K

P

P

E

E

E

D

V

L

I

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y11), V18 (≠ I16), S38 (≠ N36), G39 (= G37), S40 (≠ C38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

2oljA Abc protein artp in complex with adp/mg2+
30% identity, 83% coverage: 1:226/273 of query aligns to 3:226/242 of 2oljA

query
sites
2oljA

M

M

L

I

K

D

T

V

Q

H

S

Q

L

L

T

K

L

K

S

S

Y
x
F

G

G

D

S

S

L

I

E

I
x
V

I

L

E

K

E

G

L

I

N

N

L

V

T

H

I

I

P

R

K

E

G

G

E

E

I

V

S

V

V

V

L

V

I

I

G

G

G

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

S

C

M

L

A

N

R

L

L

L

L

E

K

D

P

F

Q

D

S

E

G

G

S

E

V

I

Q

I

L

I

N

D

G

G

K

I

E

N

I

L

S

K

R

A

Y

K

S

D

T

T

R

N

-

L

-

N

K

K

V

V

A

R

Q

E

E

E

L

V

A

G

I

M

L

V

P

F

Q

Q

S

R

P

F

T

N

A

L

P

F

E

P

G

H

L

M

T

T

V

V

L

L

Q

N

L

N

V

I

K

T

Q

L

G

A

R

-

Y

-

P

P

Y

M

Q

K

G

-

F

-

M

V

H

R

Q

K

W

W

S

P

K

R

E

E

D

K

E

A

E

E

C

A

-

K

V

A

Q

M

N

E

A

L

L

L

K

D

A

K

T

V

G

G

M

L

E

K

E

D

L

K

Q

A

D

H

R

A

F

Y

V

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

W

A

I

I

A

A

M

R

T

A

L

L

A

A

Q

M

D

E

T

P

D

K

I

I

I

M

L

L

L

F

D

D

E

E

P

P

T

T

T

S

Y

A

L

L

D

D

M

P

T

E

H

M

Q

V

I

G

E

E

I

V

L

L

D

S

L

V

L

M

F

K

D

Q

L

L

N

-

E

A

N

N

E

E

N

G

R

M

T

T

I

M

V

V

M

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

C

R

R

E

Y

V

A

G

H

D

N

R

L

V

I

L

A

F

V

M

K

D

D

G

R

G

K

Y

V

I

Y

I

Q

E

G

G

K

K

P

P

E

E

E

D

V

L

I

F

binding adenosine-5'-diphosphate: F13 (≠ Y11), V18 (≠ I16), S38 (≠ N36), G39 (= G37), S40 (≠ C38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

7chaI Cryo-em structure of p.Aeruginosa mlafebd with amppnp (see paper)
30% identity, 84% coverage: 2:229/273 of query aligns to 4:231/262 of 7chaI

query
sites
7chaI

L

V

K

E

T

L

Q

K

S

G

L

L

T

T

L

F

S

K

Y
x
R

G

G

D

S

S

R

I

A

I

I

I

F

E

D

E

N

L

I

N

D

L

V

T

R

I

I

P

P

K

R

G

G

E

K

I

V

S

T

V

G

L

I

I

M

G

G

G

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

L

L

R

R

S

L

M

I

A

A

R

S

L

Q

L

L

K

R

P

P

Q

S

S

K

G

G

S

E

V

V

Q

W

L

V

N

N

G

G

K

Q

-

N

-

L

-

P

E

Q

I

L

S

S

R

R

Y

G

S

D

T

L

R

F

K

D

V

M

A

R

Q

K

E

Q

L

F

A

G

I

V

L

L

P

F

Q

Q

S

S

P

G

T

A

A

L

P

F

E

T

G

D

L

L

T

D

V

V

L

F

Q

E

L

N

V

V

K

A

Q

-

G

-

R

-

Y

F

P

P

Y

L

Q

R

G

-

F

-

M

V

H

H

Q

T

W

Q

S

L

K

P

E

E

D

E

-

M

-

I

E

R

E

D

C

I

V

V

Q

L

N

M

A

K

L

L

K

Q

A

A

T

V

G

G

M

L

E

R

E

G

L

A

Q

V

D

E

R

L

F

M

V

P

D

D

E

E

L

L

S

S

G

G

Q

M

R

K

Q

R

R

R

A

V

W

A

I

L

A

A

M

R

T

A

L

I

A

A

Q

L

D

D

T

P

D

Q

I

I

I

L

L

L

L

Y

D

D

E

E

P

P

T

F

T

V

Y

G

L

Q

D

D

M

P

T

I

H

A

Q

M

I

G

E

V

I

L

L

V

D

R

L

L

L

I

F

R

D

L

L

L

N

N

E

D

N

A

E

L

N

G

R

I

T

T

I

S

V

I

M

V

V

V

L

S

H

H

D

D

L

L

N

A

L

E

A

T

C

A

R

S

Y

I

A

A

H

D

N

Y

L

I

I

Y

A

I

V

V

K

G

D

D

R

G

K

R

V

V

Y

L

Q

G

Q

H

G

G

K

T

P

P

E

D

E

V

V

L

I

K

S

E

C

T

D

D

binding phosphoaminophosphonic acid-adenylate ester: R13 (≠ Y11), S38 (≠ N36), G39 (= G37), C40 (= C38), G41 (= G39), K42 (= K40), T43 (≠ S41), T44 (= T42)

8j5qD Cryo-em structure of mycobacterium tuberculosis oppabcd in the pre- translocation state (see paper)
31% identity, 83% coverage: 1:227/273 of query aligns to 360:588/611 of 8j5qD

query
sites
8j5qD

M

L

L

A

K

K

T

G

Q

V

S

V

L

L

T

R

L

R

S

A

Y

I

G

G

D

E

S

V

I

R

I

A

I

V

E

D

E

G

L

I

N

S

L

L

T

E

I

L

P

R

K

Q

G

G

E

R

I

T

S

L

V

G

L

I

I

V

G

G

G

E

N

S

G

G

C

S

G

G

K

K

S

S

T

T

L

T

L

L

R

H

S

E

M

I

A

L

R

E

L

L

L

A

K

A

P

P

Q

Q

S

S

G

G

S

S

V

I

Q

E

L

V

N

L

G

G

K

T

E

D

I

V

S

A

R

T

Y

L

S

G

T

T

-

A

-

E

-

R

R

R

K

S

V

L

A

R

Q

R

E

D

L

I

A

Q

I

V

L

V

P

F

Q

Q

S

D

P

P

T

V

A

A

-

S

-

L

-

D

P

P

E

R

G

-

L

L

T

P

V

V

L

F

Q

D

L

L

V

I

K

A

Q

E

G

-

R

-

Y

-

P

P

Y

L

Q

Q

-

A

-

N

G

G

F

F

M

-

H

-

Q

-

W

G

S

K

K

N

E

E

D

T

E

H

E

A

C

R

V

V

Q

A

N

E

A

L

L

L

K

D

A

I

T

V

G

G

M

L

E

R

E

H

L

G

Q

D

-

A

D

S

R

R

F

Y

V

P

D

A

E

E

L

F

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

I

W

G

I

I

A

A

M

R

T

A

L

L

A

A

Q

L

D

Q

T

P

D

K

I

I

I

L

L

A

L

L

D

D

E

E

P

P

T

V

T

S

Y

A

L

L

D

D

M

V

T

S

H

I

Q

Q

I

A

E

G

I

I

L

I

D

N

L

L

F

L

D

D

L

L

N

Q

E

E

N

Q

E

F

N

G

R

L

T

S

I

Y

V

L

M

F

V

V

L

S

H

H

D

D

L

L

N

S

L

V

A

V

C

K

R

H

Y

L

A

A

H

H

N

Q

L

V

I

A

A

V

V

M

K

L

D

A

R

G

K

T

V

V

Y

V

Q

E

Q

Q

G

G

K

D

P

S

E

E

V

V

I

F

S

G

Sites not aligning to the query:

binding iron/sulfur cluster: 285, 291, 298, 316, 318

4fi3C Structure of vitamin b12 transporter btucd-f in a nucleotide-bound state (see paper)
31% identity, 84% coverage: 13:240/273 of query aligns to 11:234/248 of 4fi3C

query
sites
4fi3C

D

E

S

S

I

T

I
x
R

I

L

E

G

E

P

L

L

N

S

L

G

T

E

I

V

P

R

K

A

G

G

E

E

I

I

S

L

V

H

L

L

I

V

G

G

G

P

N
|
N

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

A

S

R

M

M

A

A

R

G

L

M

L

T

K

S

P

G

Q

K

S

-

G

G

S

S

V

I

Q

Q

L

F

N

A

G

G

K

Q

E

P

I

L

S

E

R

A

Y

W

S

S

T

A

R

T

K

K

V

L

A

A

Q

L

E

H

L

R

A

A

I

Y

L

L

P

S

Q
|
Q

S

Q

P

Q

T

T

A

P

P

P

E

F

G

A

L

T

T

P

V

V

L

W

Q

H

L

Y

V

L

K

T

Q

-

G

-

R

-

Y

-

P

-

Y

-

Q

-

G

-

F

-

M

L

H

H

Q

Q

W

H

S

D

K

K

E

T

D

R

E

T

E

E

C

L

V

L

Q

N

N

D

A

V

L

A

K

G

A

A

T

L

G

A

M

L

E

D

L

K

Q

L

D

G

R
|
R

F

S

V

T

D

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
x
E

R

W

Q

Q

R

R

A

V

W

R

I

L

A

A

M

A

T

V

L

V

A

L

Q

Q

D

I

T

T

-

P

-

Q

-

A

-

N

-

P

-

A

-

G

D

Q

I

L

L

L

D

D

E
x
Q

P

P

T

M

T

C

Y

S

L

L

D

D

M

V

T

A

H

Q

Q

Q

I

-

E

S

I

A

L

L

D

D

L

K

L

I

F

L

D

S

L

A

N

L

E

S

N

Q

E

Q

N

G

R

L

T

A

I

I

V

V

M

M

V

S

L

S

H

H

D

D

L

L

N

N

L

H

A

T

C

L

R

R

Y

H

A

A

H

H

N

R

L

A

I

W

A

L

V

L

K

K

D

G

R

G

K

K

V

M

Y

L

Q

A

Q

S

G

G

K

R

P

R

E

E

V

V

I

L

S

T

C

P

D

P

M

N

V

L

R

A

H

Q

V

A

F

Y

D

G

M

M

N

N

C

F

Q

R

binding phosphoaminophosphonic acid-adenylate ester: R14 (≠ I16), N34 (= N36), G35 (= G37), G37 (= G39), K38 (= K40), S39 (= S41), T40 (= T42), R121 (= R133), Q125 (≠ E137), S127 (= S139), G129 (= G141), E130 (≠ Q142), Q158 (≠ E163)
binding magnesium ion: S39 (= S41), Q79 (= Q82)

8j5tD Cryo-em structure of mycobacterium tuberculosis oppabcd in the catalytic intermediate state (see paper)
31% identity, 83% coverage: 1:227/273 of query aligns to 360:588/608 of 8j5tD

query
sites
8j5tD

M

L

L

A

K

K

T

G

Q

V

S

V

L

L

T

R

L

R

S

A

Y

I

G

G

D

E

S

V

I

R

I

A

I

V

E

D

E

G

L

I

N

S

L

L

T

E

I

L

P

R

K

Q

G

G

E

R

I

T

S

L

V

G

L

I

I

V

G

G

G

E

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

T

L

L

R

H

S

E

M

I

A

L

R

E

L

L

L

A

K

A

P

P

Q

Q

S

S

G

G

S

S

V

I

Q

E

L

V

N

L

G

G

K

T

E

D

I

V

S

A

R

T

Y

L

S

G

T

T

-

A

-

E

-

R

R

R

K

S

V

L

A

R

Q

R

E

D

L

I

A

Q

I

V

L

V

P

F

Q
|
Q

S

D

P

P

T

V

A

A

-

S

-

L

-

D

P

P

E

R

G

-

L

L

T

P

V

V

L

F

Q

D

L

L

V

I

K

A

Q

E

G

-

R

-

Y

-

P

P

Y

L

Q

Q

-

A

-

N

G

G

F

F

M

-

H

-

Q

-

W

G

S

K

K

N

E

E

D

T

E

H

E

A

C

R

V

V

Q

A

N

E

A

L

L

L

K

D

A

I

T

V

G

G

M

L

E

R

E

H

L

G

Q

D

-

A

D

S

R
|
R

F

Y

V

P

D

A

E
|
E

L

F

S
|
S

G

G

G
|
G

Q

Q

R

K

Q

Q

R

R

A

I

W

G

I

I

A

A

M

R

T

A

L

L

A

A

Q

L

D

Q

T

P

D

K

I

I

I

L

L

A

L

L

D

D

E

D

P

P

T

V

T

S

Y

A

L

L

D

D

M

V

T

S

H

I

Q

Q

I

A

E

G

I

I

L

I

D

N

L

L

F

L

D

D

L

L

N

Q

E

E

N

Q

E

F

N

G

R

L

T

S

I

Y

V

L

M

F

V

V

L

S

H
|
H

D

D

L

L

N

S

L

V

A

V

C

K

R

H

Y

L

A

A

H

H

N

Q

L

V

I

A

A

V

V

M

K

L

D

A

R

G

K

T

V

V

Y

V

Q

E

Q

Q

G

G

K

D

P

S

E

E

V

V

I

F

S

G

binding adenosine-5'-triphosphate: S395 (≠ N36), G396 (= G37), S397 (≠ C38), G398 (= G39), K399 (= K40), S400 (= S41), T401 (= T42), Q444 (= Q82), R494 (= R133), E498 (= E137), S500 (= S139), G502 (= G141), H557 (= H196)
binding magnesium ion: S400 (= S41), Q444 (= Q82)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13, 42, 43, 45, 46, 47, 48, 95, 146, 153, 155, 157, 158, 212, 358
binding magnesium ion: 47, 95
binding iron/sulfur cluster: 248, 285, 288, 291, 298, 316, 318

8j5sD Cryo-em structure of mycobacterium tuberculosis oppabcd in the pre- catalytic intermediate state (see paper)
31% identity, 83% coverage: 1:227/273 of query aligns to 360:588/608 of 8j5sD

query
sites
8j5sD

M

L

L

A

K

K

T

G

Q

V

S

V

L

L

T

R

L

R

S

A

Y

I

G

G

D

E

S

V

I

R

I
x
A

I

V

E

D

E

G

L

I

N

S

L

L

T

E

I

L

P

R

K

Q

G

G

E

R

I

T

S

L

V

G

L

I

I

V

G

G

G

E

N

S

G
|
G

C
x
S

G
|
G

K
|
K