SitesBLAST
Comparing WP_041096142.1 NCBI__GCF_000828635.1:WP_041096142.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6hulB Sulfolobus solfataricus tryptophan synthase ab complex (see paper)
48% identity, 91% coverage: 13:427/454 of query aligns to 4:412/416 of 6hulB
- binding pyridoxal-5'-phosphate: H103 (= H110), K104 (= K111), Q131 (= Q138), S220 (= S228), G255 (= G263), G256 (= G264), G257 (= G265), S258 (= S266), N259 (= N267), E371 (= E386), S400 (= S415), G401 (= G416)
- binding serine: K104 (= K111), T127 (= T134), G128 (= G135), A129 (= A136), G130 (= G137), W132 (= W139)
4qysB Trpb2 enzymes (see paper)
45% identity, 94% coverage: 3:427/454 of query aligns to 1:414/418 of 4qysB
- active site: K101 (= K111), E123 (= E133), S402 (= S415)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: H100 (= H110), K101 (= K111), G252 (= G263), G253 (= G264), G254 (= G265), S255 (= S266), N256 (= N267), E373 (= E386), S402 (= S415)
4qysA Trpb2 enzymes (see paper)
44% identity, 94% coverage: 3:427/454 of query aligns to 1:404/408 of 4qysA
- active site: K108 (= K111), E130 (= E133), S392 (= S415)
- binding pyridoxal-5'-phosphate: H107 (= H110), K108 (= K111), G242 (= G263), G243 (= G264), G244 (= G265), S245 (= S266), N246 (= N267), E363 (= E386), S392 (= S415)
- binding phosphoserine: K108 (= K111), G132 (= G135), A133 (= A136), Q135 (= Q138), W136 (= W139), G316 (= G339), R318 (= R341), E363 (= E386)
7rnpA Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9_h275e with 4-cl-trp non-covalently bound (see paper)
33% identity, 82% coverage: 50:422/454 of query aligns to 17:378/384 of 7rnpA
5vm5D Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9, with ser bound (see paper)
33% identity, 82% coverage: 50:422/454 of query aligns to 17:376/383 of 5vm5D
- active site: K82 (= K111), E104 (= E133), S369 (= S415)
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H81 (= H110), K82 (= K111), T105 (= T134), G106 (= G135), A107 (= A136), Q109 (= Q138), H110 (≠ W139), S185 (= S228), G227 (= G263), G229 (= G265), S230 (= S266), N231 (= N267), G296 (= G339), E343 (= E386), S369 (= S415)
6am8B Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9 with trp bound as e(aex2) (see paper)
33% identity, 82% coverage: 50:422/454 of query aligns to 17:378/385 of 6am8B
- active site: K82 (= K111), E104 (= E133), S371 (= S415)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-l-tryptophane: H81 (= H110), K82 (= K111), E104 (= E133), T105 (= T134), G106 (= G135), A107 (= A136), Q109 (= Q138), H110 (≠ W139), L161 (= L205), S185 (= S228), V187 (≠ L230), G227 (= G263), G228 (= G264), G229 (= G265), S230 (= S266), N231 (= N267), G298 (= G339), Y301 (= Y342), E345 (= E386), S371 (= S415), G372 (= G416)
- binding tryptophan: L169 (≠ V213), S274 (≠ T318), H275 (≠ P319)
Sites not aligning to the query:
5ixjD Tryptophan synthase beta-subunit from pyrococcus furiosus with l- threonine non-covalently bound in the active site (see paper)
33% identity, 82% coverage: 51:422/454 of query aligns to 18:376/394 of 5ixjD
5t6mA Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
33% identity, 82% coverage: 51:422/454 of query aligns to 18:376/383 of 5t6mA
5t6mB Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
32% identity, 82% coverage: 51:422/454 of query aligns to 18:378/386 of 5t6mB
1v8zA X-ray crystal structure of the tryptophan synthase b2 subunit from hyperthermophile, pyrococcus furiosus (see paper)
32% identity, 82% coverage: 51:422/454 of query aligns to 18:378/386 of 1v8zA
- active site: K82 (= K111), E104 (= E133), S371 (= S415)
- binding pyridoxal-5'-phosphate: H81 (= H110), K82 (= K111), Q109 (= Q138), S185 (= S228), G227 (= G263), G228 (= G264), G229 (= G265), S230 (= S266), N231 (= N267), E345 (= E386), S371 (= S415), G372 (= G416)
5dw0A Trpb from pyrococcus furiosus with l-serine bound as the external aldimine (see paper)
32% identity, 82% coverage: 51:422/454 of query aligns to 18:378/388 of 5dw0A
- active site: K82 (= K111), E104 (= E133), S371 (= S415)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: H81 (= H110), K82 (= K111), T105 (= T134), G106 (= G135), A107 (= A136), Q109 (= Q138), H110 (≠ W139), S185 (= S228), G227 (= G263), G229 (= G265), S230 (= S266), N231 (= N267), G298 (= G339), D300 (≠ R341), E345 (= E386), S371 (= S415)
5dw3A Tryptophan synthase beta-subunit from pyrococcus furiosus with product l-tryptophan non-covalently bound in the active site (see paper)
33% identity, 82% coverage: 51:422/454 of query aligns to 18:377/383 of 5dw3A
- active site: K82 (= K111), E104 (= E133), S370 (= S415)
- binding tryptophan: K82 (= K111), E104 (= E133), T105 (= T134), G106 (= G135), A107 (= A136), Q109 (= Q138), H110 (≠ W139), S185 (= S228), G228 (= G264), Y300 (= Y342)
6cutA Engineered holo trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3s)- isopropylserine bound as the external aldimine (see paper)
33% identity, 82% coverage: 50:422/454 of query aligns to 17:378/385 of 6cutA
- binding (2S,3S)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic acid (non-preferred name): H81 (= H110), K82 (= K111), T105 (= T134), G106 (= G135), A107 (= A136), Q109 (= Q138), H110 (≠ W139), S185 (= S228), G227 (= G263), G229 (= G265), S230 (= S266), N231 (= N267), G298 (= G339), E345 (= E386), S371 (= S415)
6cuzA Engineered trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3r)- ethylserine bound as the amino-acrylate (see paper)
33% identity, 82% coverage: 50:422/454 of query aligns to 17:378/383 of 6cuzA
- binding (2E)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]pent-2-enoic acid: H81 (= H110), K82 (= K111), T105 (= T134), G106 (= G135), A107 (= A136), Q109 (= Q138), H110 (≠ W139), S185 (= S228), G227 (= G263), G229 (= G265), S230 (= S266), N231 (= N267), G298 (= G339), E345 (= E386), S371 (= S415)
6usaB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk1-bound form (see paper)
30% identity, 88% coverage: 31:430/454 of query aligns to 18:401/404 of 6usaB
- active site: K97 (= K111), E119 (= E133), S386 (= S415)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H110), K97 (= K111), T120 (= T134), G121 (= G135), A122 (= A136), G123 (= G137), Q124 (= Q138), H125 (≠ W139), T200 (≠ S228), G242 (= G263), G244 (= G265), S245 (= S266), N246 (= N267), G313 (= G339), E360 (= E386), S386 (= S415)
- binding (3R,4R)-4-[4-(2-Chlorophenyl)piperazin-1-yl]-1,1-dioxothiolan-3-ol: F184 (≠ V213), W187 (≠ T216), Y196 (= Y224), F198 (≠ L226), G203 (vs. gap), P204 (vs. gap), F207 (vs. gap), H290 (≠ K304), G291 (= G305)
6dweB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and brd0059-bound form
30% identity, 88% coverage: 31:430/454 of query aligns to 18:401/404 of 6dweB
- active site: K97 (= K111), E119 (= E133), S386 (= S415)
- binding (2R,3S,4R)-3-(2',6'-difluoro-4'-methyl[1,1'-biphenyl]-4-yl)-4-(fluoromethyl)azetidine-2-carbonitrile: F184 (≠ V213), Y196 (= Y224), F198 (≠ L226), P204 (vs. gap), F207 (vs. gap), H290 (≠ K304)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H110), K97 (= K111), T120 (= T134), G121 (= G135), A122 (= A136), G123 (= G137), Q124 (= Q138), H125 (≠ W139), T200 (≠ S228), G242 (= G263), G244 (= G265), S245 (= S266), N246 (= N267), G313 (= G339), E360 (= E386), S386 (= S415)
6u6cB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk2-bound form (see paper)
30% identity, 88% coverage: 31:430/454 of query aligns to 19:402/405 of 6u6cB
- active site: K98 (= K111), E120 (= E133), S387 (= S415)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H97 (= H110), K98 (= K111), T121 (= T134), G122 (= G135), A123 (= A136), Q125 (= Q138), H126 (≠ W139), T201 (≠ S228), G243 (= G263), G245 (= G265), S246 (= S266), N247 (= N267), G314 (= G339), E361 (= E386), S387 (= S415)
- binding 1-(2-fluorobenzene-1-carbonyl)-N-methyl-2,3-dihydro-1H-indole-5-sulfonamide: Y26 (≠ P38), F185 (≠ V213), W188 (≠ T216), Y197 (= Y224), F199 (≠ L226), G204 (vs. gap), P205 (vs. gap), H291 (≠ K304), G292 (= G305)
5tciH Crystal structure of tryptophan synthase from m. Tuberculosis - brd4592-bound form (see paper)
30% identity, 88% coverage: 31:430/454 of query aligns to 19:402/406 of 5tciH
- active site: K98 (= K111), E120 (= E133), S387 (= S415)
- binding (2R,3S,4R)-3-(2'-fluoro[1,1'-biphenyl]-4-yl)-4-(hydroxymethyl)azetidine-2-carbonitrile: P28 (= P41), L31 (≠ M44), Y197 (= Y224), F199 (≠ L226), P205 (vs. gap), F208 (vs. gap), H291 (≠ K304)
6uapB Crystal structure of tryptophan synthase from m. Tuberculosis - open form with brd6309 bound
30% identity, 88% coverage: 31:430/454 of query aligns to 18:401/405 of 6uapB
- active site: K97 (= K111), E119 (= E133), S386 (= S415)
- binding (2R,3S,4R)-3-(4'-chloro-2',6'-difluoro[1,1'-biphenyl]-4-yl)-4-(fluoromethyl)azetidine-2-carbonitrile: I180 (= I209), N181 (≠ S210), F184 (≠ V213), Y196 (= Y224), F198 (≠ L226), P204 (vs. gap), F207 (vs. gap), H290 (≠ K304)
5ocwB Structure of mycobacterium tuberculosis tryptophan synthase in space group f222 (see paper)
30% identity, 88% coverage: 31:430/454 of query aligns to 14:397/399 of 5ocwB
- active site: K93 (= K111), E115 (= E133), S382 (= S415)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H92 (= H110), K93 (= K111), T116 (= T134), G117 (= G135), A118 (= A136), Q120 (= Q138), H121 (≠ W139), T196 (≠ S228), G238 (= G263), G240 (= G265), S241 (= S266), N242 (= N267), G309 (= G339), E356 (= E386), S382 (= S415)
Query Sequence
>WP_041096142.1 NCBI__GCF_000828635.1:WP_041096142.1
MSQTRIVLDQSEIPTHWYNIAADLKNPPSPPLGPDGKPVGPEQMLAIFPGPILEQEMSAE
RWIKIPDEVRQIYALWRPSPLCRAVRLEQMLGTPAKIFYKYEGVSPSGSHKPNSAVPQAY
LNKIAGTQQLLTETGAGQWGSSLAFAGQMFGLPVKIFMVKVSYDQKPYRRLMMQTFGGEV
VASPSNQTKTGRDILAADPDNQGSLGIAISEAVEATVTTPGSKYTLGSVLNHVLLHQTIV
GQEAKKQFEKFGEYPDMIFAPCGGGSNFAGVSFPFLGDAAAGDKRAQKIRFVAVEPASCP
TLTKGVYAYDYGDATGFTPLMKMYTLGHDFMPPGIHAGGLRYHGDSPLVSQLYNEKLIEA
VAVPQTATFEAGVMFARAEGIIPAPESAHGIRACIDEALRCKASGEPKTLFFNLSGHGHF
DMTAYESYFGGKLQDFEYPAEAIQASLARLPKVG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory