SitesBLAST
Comparing WP_041096374.1 NCBI__GCF_000828635.1:WP_041096374.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6rxcD Leishmania major pteridine reductase 1 (lmptr1) in complex with inhibitor 4 (nmt-c0026) (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 2:252/256 of 6rxcD
- active site: R13 (= R18), D155 (= D144), Y168 (= Y157)
- binding methyl 1-[4-[[2,4-bis(azanyl)pteridin-6-yl]methyl-(3-oxidanylpropyl)amino]phenyl]carbonylpiperidine-4-carboxylate: S99 (= S95), F101 (= F97), Y165 (= Y154), Y168 (= Y157), L200 (vs. gap)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), L14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), R35 (≠ S40), S36 (= S41), D61 (= D66), L62 (= L67), N97 (= N93), A98 (= A94), S99 (= S95), S100 (= S96), M153 (≠ I142), D155 (= D144), K172 (= K161), P198 (= P186), S201 (vs. gap)
6rxcA Leishmania major pteridine reductase 1 (lmptr1) in complex with inhibitor 4 (nmt-c0026) (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 2:259/263 of 6rxcA
- active site: R13 (= R18), D159 (= D144), Y172 (= Y157)
- binding methyl 1-[4-[[2,4-bis(azanyl)pteridin-6-yl]methyl-(3-oxidanylpropyl)amino]phenyl]carbonylpiperidine-4-carboxylate: S100 (= S95), F102 (= F97), L166 (= L151), Y169 (= Y154), Y172 (= Y157), L204 (vs. gap)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), L14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), R35 (≠ S40), S36 (= S41), L62 (= L67), N98 (= N93), A99 (= A94), S100 (= S95), S101 (= S96), S124 (= S116), M157 (≠ I142), D159 (= D144), K176 (= K161), P202 (= P186), S205 (vs. gap)
7pxxA The crystal structure of leishmania major pteridine reductase 1 in complex with substrate folic acid (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 2:260/264 of 7pxxA
- binding folic acid: S100 (= S95), F102 (= F97), L164 (= L151), Y167 (= Y154), Y170 (= Y157), L202 (vs. gap), H217 (≠ N194)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), L14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), R35 (≠ S40), S36 (= S41), L62 (= L67), N98 (= N93), A99 (= A94), S100 (= S95), S101 (= S96), M155 (≠ I142), V156 (≠ T143), D157 (= D144), Y170 (= Y157), K174 (= K161), P200 (= P186), G201 (= G187), S203 (vs. gap)
2qhxA Structure of pteridine reductase from leishmania major complexed with a ligand (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 2:260/264 of 2qhxA
- active site: R13 (= R18), R35 (≠ S40), N41 (≠ E46), N97 (= N92), D157 (= D144), Q162 (≠ R149), Y170 (= Y157)
- binding methyl 1-(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)amino}benzoyl)piperidine-4-carboxylate: S100 (= S95), F102 (= F97), L164 (= L151), Y170 (= Y157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), L14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), R35 (≠ S40), S36 (= S41), L62 (= L67), N98 (= N93), A99 (= A94), S100 (= S95), S101 (= S96), M155 (≠ I142), V156 (≠ T143), K174 (= K161), P200 (= P186), L202 (vs. gap), S203 (vs. gap)
4clhA Crystal structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor and inhibitor (see paper)
40% identity, 95% coverage: 8:245/250 of query aligns to 3:246/252 of 4clhA
- active site: R13 (= R18), D145 (= D144), Y158 (= Y157), K162 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), I14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), N35 (≠ S40), S36 (= S41), D61 (= D66), L62 (= L67), N92 (= N93), A93 (= A94), S94 (= S95), T117 (≠ S116), L143 (≠ I142), K162 (= K161), P188 (= P186), G189 (= G187), S191 (vs. gap)
- binding 4-thiomorpholino-7H-pyrrolo[2,3-d]pyrimidin-2-amine: S94 (= S95), F96 (= F97), F96 (= F97), D145 (= D144), C152 (≠ L151), F155 (≠ Y154), Y158 (= Y157), P194 (= P188), W205 (≠ A202)
4cleA Crystal structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor and inhibitor (see paper)
40% identity, 95% coverage: 8:245/250 of query aligns to 3:246/252 of 4cleA
- active site: R13 (= R18), D145 (= D144), Y158 (= Y157), K162 (= K161)
- binding 4-(pyrrolidin-1-yl)-7H-pyrrolo[2,3-d]pyrimidin-2-amine: S94 (= S95), F96 (= F97), F96 (= F97), D145 (= D144), C152 (≠ L151), Y158 (= Y157), G189 (= G187), P194 (= P188), W205 (≠ A202)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), I14 (≠ V19), Y33 (≠ C38), H34 (≠ R39), N35 (≠ S40), S36 (= S41), L62 (= L67), N92 (= N93), A93 (= A94), S94 (= S95), T117 (≠ S116), K162 (= K161), P188 (= P186), S191 (vs. gap)
5l42A Leishmania major pteridine reductase 1 (ptr1) in complex with compound 3 (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 2:261/265 of 5l42A
- active site: R13 (= R18), D158 (= D144), Y171 (= Y157)
- binding (2~{R})-2-[3,4-bis(oxidanyl)phenyl]-6-oxidanyl-2,3-dihydrochromen-4-one: F102 (= F97), Y171 (= Y157), G202 (= G187), L203 (vs. gap), Y260 (≠ R246)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), L14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), R35 (≠ S40), S36 (= S41), L62 (= L67), N98 (= N93), A99 (= A94), S100 (= S95), S101 (= S96), M156 (≠ I142), D158 (= D144), K175 (= K161), L203 (vs. gap), S204 (vs. gap)
5l4nA Leishmania major pteridine reductase 1 (ptr1) in complex with compound 1 (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 2:250/254 of 5l4nA
- active site: R13 (= R18), D156 (= D144), Y169 (= Y157)
- binding (2~{R})-2-(3-hydroxyphenyl)-6-oxidanyl-2,3-dihydrochromen-4-one: S100 (= S95), F102 (= F97), Y169 (= Y157), L201 (≠ P188)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), L14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), R35 (≠ S40), S36 (= S41), D61 (= D66), L62 (= L67), N98 (= N93), A99 (= A94), S100 (= S95), S101 (= S96), M154 (≠ I142), D156 (= D144), K173 (= K161), P199 (= P186), S202 (≠ I189)
2bfmA Leishmania major pteridine reductase 1 in complex with NADP and trimethoprim (see paper)
39% identity, 96% coverage: 7:247/250 of query aligns to 1:262/266 of 2bfmA
- active site: R12 (= R18), D159 (= D144), Y172 (= Y157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R12 (= R18), L13 (≠ V19), Y32 (≠ C38), H33 (≠ R39), R34 (≠ S40), S35 (= S41), L61 (= L67), N97 (= N93), A98 (= A94), S99 (= S95), M157 (≠ I142), V158 (≠ T143), D159 (= D144), K176 (= K161), P202 (= P186), L204 (vs. gap), S205 (vs. gap)
- binding trimethoprim: F101 (= F97), L166 (= L151), Y172 (= Y157), L204 (vs. gap), H219 (≠ N194)
7pxxC The crystal structure of leishmania major pteridine reductase 1 in complex with substrate folic acid (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 1:249/253 of 7pxxC
- binding folic acid: S99 (= S95), F101 (= F97), Y168 (= Y157), L200 (≠ P188)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R12 (= R18), L13 (≠ V19), H31 (= H37), Y32 (≠ C38), H33 (≠ R39), R34 (≠ S40), S35 (= S41), D60 (= D66), L61 (= L67), N97 (= N93), A98 (= A94), S99 (= S95), S100 (= S96), M153 (≠ I142), D155 (= D144), Y168 (= Y157), K172 (= K161), P198 (= P186), G199 (= G187), L200 (≠ P188), S201 (≠ I189)
2qhxC Structure of pteridine reductase from leishmania major complexed with a ligand (see paper)
40% identity, 96% coverage: 7:247/250 of query aligns to 1:250/254 of 2qhxC
- active site: R12 (= R18), R34 (≠ S40), N40 (≠ E46), N96 (= N92), D155 (= D144), Q160 (≠ R149), Y168 (= Y157)
- binding methyl 1-(4-{[(2,4-diaminopteridin-6-yl)methyl](methyl)amino}benzoyl)piperidine-4-carboxylate: S99 (= S95), F101 (= F97), Y165 (= Y154), Y168 (= Y157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R12 (= R18), L13 (≠ V19), H31 (= H37), Y32 (≠ C38), H33 (≠ R39), R34 (≠ S40), S35 (= S41), L61 (= L67), N97 (= N93), A98 (= A94), S99 (= S95), S100 (= S96), M153 (≠ I142), V154 (≠ T143), D155 (= D144), K172 (= K161), P198 (= P186), L200 (≠ P188), S201 (≠ I189)
3gn1C Structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor (NADP+) and inhibitor (ddd00067116) (see paper)
41% identity, 95% coverage: 8:245/250 of query aligns to 3:242/248 of 3gn1C
- active site: R13 (= R18), D141 (= D144), Y154 (= Y157), K158 (= K161)
- binding 1H-benzimidazol-2-amine: F96 (= F97), Y154 (= Y157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), I14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), N35 (≠ S40), S36 (= S41), D61 (= D66), L62 (= L67), N92 (= N93), A93 (= A94), S94 (= S95), T116 (≠ S116), L139 (≠ I142), C140 (≠ T143), D141 (= D144), K158 (= K161), P184 (= P186), G185 (= G187), V186 (vs. gap), S187 (vs. gap)
1p33B Pteridine reductase from leishmania tarentolae complex with NADPH and mtx (see paper)
40% identity, 97% coverage: 4:245/250 of query aligns to 1:263/269 of 1p33B
- active site: R15 (= R18), D162 (= D144), Y175 (= Y157)
- binding methotrexate: R15 (= R18), S103 (= S95), F105 (= F97), Y172 (= Y154), Y175 (= Y157), L207 (≠ P188), P211 (= P192), M214 (≠ Q195)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R15 (= R18), L16 (≠ V19), H36 (≠ R39), R37 (≠ S40), S38 (= S41), D63 (= D66), L64 (= L67), N101 (= N93), A102 (= A94), S103 (= S95), S104 (= S96), M160 (≠ I142), V161 (≠ T143), K179 (= K161), G206 (= G187), S208 (≠ I189), V209 (≠ E190)
3jq7C Crystal structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor (NADP+) and inhibitor 6- phenylpteridine-2,4,7-triamine (dx2) (see paper)
41% identity, 95% coverage: 8:245/250 of query aligns to 2:233/239 of 3jq7C
- active site: R12 (= R18), D140 (= D144), Y153 (= Y157), K157 (= K161)
- binding 6-phenylpteridine-2,4,7-triamine: R12 (= R18), S93 (= S95), F95 (= F97), Y153 (= Y157), V185 (≠ P188), L188 (≠ W191), P189 (= P192)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R12 (= R18), I13 (≠ V19), Y32 (≠ C38), H33 (≠ R39), N34 (≠ S40), S35 (= S41), D60 (= D66), L61 (= L67), N91 (= N93), A92 (= A94), S93 (= S95), T115 (≠ S116), L138 (≠ I142), C139 (≠ T143), D140 (= D144), K157 (= K161), G184 (= G187), V185 (≠ P188), S186 (≠ I189), L187 (≠ E190)
1e7wA One active site, two modes of reduction correlate the mechanism of leishmania pteridine reductase with pterin metabolism and antifolate drug resistance in trpanosomes (see paper)
39% identity, 96% coverage: 7:247/250 of query aligns to 2:263/267 of 1e7wA
- active site: R13 (= R18), D160 (= D144), Y173 (= Y157)
- binding methotrexate: S103 (= S95), F105 (= F97), L167 (= L151), Y170 (= Y154), Y173 (= Y157), D211 (vs. gap), M212 (vs. gap)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), L14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), R35 (≠ S40), S36 (= S41), L62 (= L67), N101 (= N93), A102 (= A94), S103 (= S95), M158 (≠ I142), D160 (= D144), K177 (= K161), P203 (= P186), L205 (vs. gap), S206 (vs. gap)
3jq8A Crystal structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor (NADP+) and inhibitor 6,7,7- trimethyl-7,8-dihydropteridine-2,4-diamine (dx3) (see paper)
41% identity, 95% coverage: 8:245/250 of query aligns to 3:242/248 of 3jq8A
- active site: R13 (= R18), D142 (= D144), Y155 (= Y157), K159 (= K161)
- binding 6,7,7-trimethyl-7,8-dihydropteridine-2,4-diamine: S94 (= S95), F96 (= F97), Y155 (= Y157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), I14 (≠ V19), H32 (= H37), Y33 (≠ C38), H34 (≠ R39), N35 (≠ S40), S36 (= S41), D61 (= D66), L62 (= L67), N92 (= N93), A93 (= A94), S94 (= S95), T116 (≠ S116), C141 (≠ T143), D142 (= D144), K159 (= K161), P185 (= P186), G186 (= G187), S188 (≠ I189)
2bfpA Leishmania major pteridine reductase 1 in complex with NADP and tetrahydrobiopterin (see paper)
39% identity, 96% coverage: 7:247/250 of query aligns to 1:263/267 of 2bfpA
- active site: R12 (= R18), D160 (= D144), Y173 (= Y157)
- binding 5,6,7,8-tetrahydrobiopterin: S99 (= S95), F101 (= F97), Y173 (= Y157), L205 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R12 (= R18), L13 (≠ V19), Y32 (≠ C38), H33 (≠ R39), R34 (≠ S40), S35 (= S41), L61 (= L67), N97 (= N93), A98 (= A94), S99 (= S95), S100 (= S96), M158 (≠ I142), K177 (= K161), P203 (= P186), L205 (vs. gap), S206 (vs. gap)
4cmiA Crystal structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor and inhibitor (see paper)
41% identity, 95% coverage: 8:245/250 of query aligns to 3:244/250 of 4cmiA
- active site: R13 (= R18), D143 (= D144), Y156 (= Y157), K160 (= K161)
- binding 2-amino-6-(4-bromophenyl)-5-phenyl-3H-pyrrolo[2,3-d]pyrimidin-4(7H)-one: S94 (= S95), F96 (= F97), M145 (≠ H146), C150 (≠ L151), Y156 (= Y157), L191 (vs. gap), P192 (= P188)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), I14 (≠ V19), Y33 (≠ C38), H34 (≠ R39), N35 (≠ S40), S36 (= S41), L62 (= L67), N92 (= N93), S94 (= S95), T116 (≠ S116), L141 (≠ I142), C142 (≠ T143), K160 (= K161), P186 (= P186), G187 (= G187), S189 (vs. gap)
4cmgA Crystal structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor and inhibitor (see paper)
41% identity, 95% coverage: 8:245/250 of query aligns to 3:244/250 of 4cmgA
- active site: R13 (= R18), D143 (= D144), Y156 (= Y157), K160 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), I14 (≠ V19), Y33 (≠ C38), H34 (≠ R39), N35 (≠ S40), S36 (= S41), D61 (= D66), L62 (= L67), N92 (= N93), A93 (= A94), S94 (= S95), T116 (≠ S116), L141 (≠ I142), C142 (≠ T143), K160 (= K161), P186 (= P186), V188 (vs. gap), S189 (vs. gap)
- binding 6-(4-fluorophenyl)-5-(4-methoxyphenyl)-7H-pyrrolo[2,3-d]pyrimidine-2,4-diamine: S94 (= S95), F96 (= F97), M145 (≠ H146), C150 (≠ L151), Y156 (= Y157), G187 (= G187), L191 (vs. gap), M195 (≠ W191), W203 (≠ A202)
4cmaA Crystal structure of pteridine reductase 1 (ptr1) from trypanosoma brucei in ternary complex with cofactor and inhibitor (see paper)
41% identity, 95% coverage: 8:245/250 of query aligns to 3:244/250 of 4cmaA
- active site: R13 (= R18), D143 (= D144), Y156 (= Y157), K160 (= K161)
- binding 5,6-diphenyl-7H-pyrrolo[2,3-d]pyrimidine-2,4-diamine: S94 (= S95), F96 (= F97), D143 (= D144), M145 (≠ H146), C150 (≠ L151), Y156 (= Y157), G187 (= G187), L191 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R13 (= R18), I14 (≠ V19), H34 (≠ R39), N35 (≠ S40), S36 (= S41), D61 (= D66), L62 (= L67), N92 (= N93), A93 (= A94), S94 (= S95), T116 (≠ S116), L141 (≠ I142), K160 (= K161), P186 (= P186), V188 (vs. gap), S189 (vs. gap)
Query Sequence
>WP_041096374.1 NCBI__GCF_000828635.1:WP_041096374.1
MTNSPRLPVVLVTGAARRVGAEIARSLHAAGARVAIHCRSSVAEAEALAAELNGARADSA
AVFAADLLDSAALPGLVESVVARFGRLDALVNNASSFFATKVGAVDTAAWDDLIGSNLKA
PLFLSQAAAPHLERSGGCIVNITDIHAERPLKGYPLYCAAKAGLLGLSRALALELGPRVR
VNAVAPGPIEWPQNQHDFPPDARTAIIEHTLLKRIGSPADIARTVKFLVFDAPYVTGQVI
NVDGGRTAHL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory