SitesBLAST
Comparing WP_041097743.1 NCBI__GCF_000828635.1:WP_041097743.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
68% identity, 100% coverage: 3:697/697 of query aligns to 1:691/692 of 6iunB
- active site: A60 (= A62), F65 (= F67), E73 (= E75), H77 (≠ L79), G101 (= G103), E104 (= E106), E124 (= E126), G132 (= G134), K248 (= K254), S407 (= S413), H428 (= H434), E440 (= E446), N478 (= N484)
- binding nicotinamide-adenine-dinucleotide: G300 (= G306), T301 (= T307), M302 (= M308), E321 (= E327), T322 (≠ M328), Y365 (= Y371), A377 (= A383), V378 (= V384), E380 (= E386), V384 (= V390), V388 (= V394), N405 (= N411), S407 (= S413)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
42% identity, 99% coverage: 7:693/697 of query aligns to 6:705/723 of Q08426
- V40 (≠ T41) to G: in dbSNP:rs1062551
- I41 (≠ A42) to R: in dbSNP:rs1062552
- T75 (≠ N77) to I: in dbSNP:rs1062553
- K165 (≠ G167) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ E173) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (≠ G278) to T: in dbSNP:rs2302819
- A325 (≠ M325) to G: in dbSNP:rs1062555
- K346 (≠ N343) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (≠ Q573) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ T587) to T: in dbSNP:rs1042437
- T606 (≠ E595) to P: in dbSNP:rs1042438
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh'
41% identity, 99% coverage: 7:693/697 of query aligns to 11:707/723 of 6zibAAA
- active site: A66 (= A62), F71 (= F67), G81 (vs. gap), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), G136 (= G134), K254 (= K254), S413 (= S413), H434 (= H434), E446 (= E446), N484 (= N484)
- binding acetoacetyl-coenzyme a: P25 (= P21), V26 (= V22), A64 (≠ G60), G65 (= G61), A66 (= A62), D67 (= D63), I68 (= I64), G104 (= G102), G105 (= G103), E128 (= E126), Y161 (≠ P159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G310 (= G306), T311 (= T307), M312 (= M308), E331 (= E327), S332 (≠ M328), Q336 (≠ A332), A383 (= A383), V384 (= V384), F385 (= F385), E386 (= E386), N411 (= N411), H434 (= H434)
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
41% identity, 99% coverage: 7:693/697 of query aligns to 11:709/725 of 5omoA
- active site: A66 (= A62), F71 (= F67), G81 (vs. gap), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K254), S415 (= S413), H436 (= H434), E448 (= E446), N486 (= N484)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P21), V26 (= V22), A28 (≠ G24), P31 (≠ Y27), A64 (≠ G60), A66 (= A62), D67 (= D63), I68 (= I64), L103 (≠ M101), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), Y161 (≠ P159), F260 (= F260), K280 (≠ R280)
- binding 3-keto-decanoyl-coa: S415 (= S413), N486 (= N484), K519 (≠ A517), M520 (= M518), V525 (≠ M523), Y658 (= Y643)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
41% identity, 99% coverage: 7:693/697 of query aligns to 11:709/725 of 5mgbA
- active site: A66 (= A62), F71 (= F67), G81 (vs. gap), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K254), S415 (= S413), H436 (= H434), E448 (= E446), N486 (= N484)
- binding acetoacetyl-coenzyme a: P25 (= P21), V26 (= V22), A64 (≠ G60), G65 (= G61), A66 (= A62), D67 (= D63), I68 (= I64), G105 (= G103), E128 (= E126), Y161 (≠ P159)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ I303), G308 (= G304), G310 (= G306), T311 (= T307), M312 (= M308), E331 (= E327), S332 (≠ M328), Q336 (≠ A332), V386 (= V384), F387 (= F385), E388 (= E386), N413 (= N411), S415 (= S413), H436 (= H434)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
41% identity, 99% coverage: 7:693/697 of query aligns to 11:709/725 of 3zwcA
- active site: A66 (= A62), F71 (= F67), G81 (vs. gap), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K254), S415 (= S413), H436 (= H434), E448 (= E446), N486 (= N484)
- binding (s)-3-hydroxydecanoyl-coa: V26 (= V22), A64 (≠ G60), G65 (= G61), A66 (= A62), D67 (= D63), I68 (= I64), G77 (≠ N77), L78 (= L78), L80 (vs. gap), V101 (= V99), G104 (= G102), G105 (= G103), E108 (= E106), E128 (= E126), F260 (= F260)
- binding nicotinamide-adenine-dinucleotide: G308 (= G304), G310 (= G306), T311 (= T307), M312 (= M308), E331 (= E327), Q336 (≠ A332), A385 (= A383), V386 (= V384), F387 (= F385), E388 (= E386), K393 (= K391), N413 (= N411), S415 (= S413), H436 (= H434)
2x58A The crystal structure of mfe1 liganded with coa (see paper)
41% identity, 99% coverage: 7:693/697 of query aligns to 11:709/725 of 2x58A
- active site: A66 (= A62), F71 (= F67), G81 (vs. gap), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K254), S415 (= S413), H436 (= H434), E448 (= E446), N486 (= N484)
- binding adenosine-5'-diphosphate: G310 (= G306), T311 (= T307), M312 (= M308), E331 (= E327), S332 (≠ M328), Q336 (≠ A332), V386 (= V384), L392 (≠ V390)
- binding coenzyme a: V26 (= V22), A28 (≠ G24), A64 (≠ G60), A66 (= A62), D67 (= D63), I68 (= I64), E128 (= E126)
3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
41% identity, 99% coverage: 7:693/697 of query aligns to 9:707/723 of 3zw9A
- active site: A64 (= A62), F69 (= F67), G79 (vs. gap), G103 (= G103), E106 (= E106), P125 (= P125), E126 (= E126), P133 (= P133), G134 (= G134), K252 (= K254), S413 (= S413), H434 (= H434), E446 (= E446), N484 (= N484)
- binding nicotinamide-adenine-dinucleotide: L305 (≠ I303), G306 (= G304), G308 (= G306), T309 (= T307), M310 (= M308), E329 (= E327), Q334 (≠ A332), A383 (= A383), V384 (= V384), F385 (= F385), E386 (= E386), N411 (= N411), S413 (= S413), H434 (= H434)
- binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (= V22), A62 (≠ G60), G63 (= G61), A64 (= A62), I66 (= I64), G102 (= G102), G103 (= G103), E106 (= E106), E126 (= E126), P133 (= P133), Y159 (≠ P159)
3zwaA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-hexanoyl-coa (see paper)
41% identity, 99% coverage: 7:693/697 of query aligns to 12:710/727 of 3zwaA
- active site: A67 (= A62), F72 (= F67), G82 (vs. gap), G106 (= G103), E109 (= E106), P128 (= P125), E129 (= E126), P136 (= P133), G137 (= G134), K255 (= K254), S416 (= S413), H437 (= H434), E449 (= E446), N487 (= N484)
- binding (S)-3-Hydroxyhexanoyl-CoA: V27 (= V22), A65 (≠ G60), G66 (= G61), A67 (= A62), D68 (= D63), I69 (= I64), L104 (≠ M101), E109 (= E106), R124 (≠ S121), E129 (= E126), L132 (= L129), G137 (= G134), Y162 (≠ P159)
6zicAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with 3s-hydroxybutanoyl-coa and nadh'
41% identity, 99% coverage: 7:693/697 of query aligns to 11:707/723 of 6zicAAA
- active site: A66 (= A62), F71 (= F67), G81 (vs. gap), G105 (= G103), E108 (= E106), P127 (= P125), E128 (= E126), G136 (= G134), K254 (= K254), S413 (= S413), H434 (= H434), E446 (= E446), N484 (= N484)
- binding 3-hydroxybutanoyl-coenzyme a: P25 (= P21), V26 (= V22), A28 (≠ G24), A66 (= A62), D67 (= D63), I68 (= I64), G104 (= G102), G105 (= G103), E108 (= E106), E128 (= E126), Y161 (≠ P159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G308 (= G304), G310 (= G306), T311 (= T307), M312 (= M308), E331 (= E327), S332 (≠ M328), Q336 (≠ A332), A383 (= A383), V384 (= V384), F385 (= F385), E386 (= E386), L390 (≠ V390), K391 (= K391), N411 (= N411), S413 (= S413), H434 (= H434)
6z5oAAA Peroxisomal bifunctional enzyme (see paper)
40% identity, 99% coverage: 7:693/697 of query aligns to 12:703/716 of 6z5oAAA
- active site: A67 (= A62), F72 (= F67), G82 (vs. gap), G106 (= G103), E109 (= E106), P128 (= P125), E129 (= E126), G137 (= G134), K255 (= K254), S409 (= S413), H430 (= H434), E442 (= E446), N480 (= N484)
- binding coenzyme a: P26 (= P21), V27 (= V22), A65 (≠ G60), D68 (= D63), I69 (= I64), P128 (= P125), Y162 (≠ P159), F277 (= F276), K281 (≠ R280)
- binding nicotinamide-adenine-dinucleotide: G309 (= G304), G311 (= G306), T312 (= T307), M313 (= M308), E332 (= E327), S333 (≠ M328), Q337 (≠ A332), A379 (= A383), V380 (= V384), F381 (= F385), E382 (= E386), K387 (= K391), N407 (= N411), S409 (= S413), H430 (= H434)
- binding nicotinamide: A67 (= A62), E109 (= E106), E129 (= E126), P136 (= P133), F261 (= F260)
3zwbA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa (see paper)
41% identity, 99% coverage: 7:693/697 of query aligns to 11:709/725 of 3zwbA
- active site: A66 (= A62), G81 (vs. gap), G105 (= G103), E108 (= E106), P127 (= P125), A128 (≠ E126), P135 (= P133), G136 (= G134), S415 (= S413), H436 (= H434), E448 (= E446), N486 (= N484)
- binding (2E)-Hexenoyl-CoA: P25 (= P21), V26 (= V22), A28 (≠ G24), A64 (≠ G60), G65 (= G61), A66 (= A62), D67 (= D63), I68 (= I64), V101 (= V99), L103 (≠ M101), G105 (= G103), E108 (= E106), G136 (= G134), Y161 (≠ P159), K280 (≠ R280)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
31% identity, 98% coverage: 2:687/697 of query aligns to 39:751/763 of P40939
- V282 (≠ A215) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- I305 (= I238) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- L342 (≠ A275) to P: in LCHAD deficiency; dbSNP:rs137852772
- E510 (= E446) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
33% identity, 95% coverage: 37:696/697 of query aligns to 44:713/715 of 1wdlA
- active site: A69 (= A62), N89 (vs. gap), N93 (vs. gap), G117 (= G103), E120 (= E106), P139 (= P125), E140 (= E126), P147 (= P133), G148 (= G134), S430 (= S413), H451 (= H434), E463 (= E446), N501 (= N484)
- binding nicotinamide-adenine-dinucleotide: A322 (= A305), I324 (≠ T307), M325 (= M308), D344 (≠ E327), I345 (≠ M328), A400 (= A383), V401 (= V384), E403 (= E386), N428 (= N411), T429 (= T412), S430 (= S413)
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
33% identity, 95% coverage: 37:696/697 of query aligns to 44:713/715 of P28793
- D297 (≠ E279) binding substrate
- M325 (= M308) binding NAD(+)
- D344 (≠ E327) binding NAD(+)
- VVE 401:403 (≠ VFE 384:386) binding NAD(+)
- K408 (= K391) binding NAD(+)
- S430 (= S413) binding NAD(+)
- N454 (≠ S437) binding NAD(+)
- N501 (= N484) binding substrate
- Y660 (≠ T641) binding substrate
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
33% identity, 95% coverage: 37:696/697 of query aligns to 44:705/707 of 1wdmA
- active site: A69 (= A62), N89 (vs. gap), N93 (vs. gap), G117 (= G103), E120 (= E106), P139 (= P125), E140 (= E126), P147 (= P133), G148 (= G134), S430 (= S413), H451 (= H434), E463 (= E446), N501 (= N484)
- binding acetyl coenzyme *a: K142 (= K128), D297 (≠ E279), M459 (= M442), N501 (= N484), P534 (≠ A517), Y652 (≠ T641), L658 (= L647)
- binding nicotinamide-adenine-dinucleotide: G321 (= G304), A322 (= A305), I324 (≠ T307), M325 (= M308), D344 (≠ E327), V401 (= V384), E403 (= E386), N428 (= N411), S430 (= S413), N454 (≠ S437)
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
31% identity, 98% coverage: 11:696/697 of query aligns to 12:702/707 of 6yswA
- active site: A66 (= A62), I71 (≠ F67), A84 (≠ T80), Q88 (vs. gap), G112 (= G103), E115 (= E106), P136 (= P125), E137 (= E126), G145 (= G134), D264 (≠ K254), S422 (= S413), H443 (= H434), E455 (= E446), N493 (= N484)
- binding coenzyme a: E23 (vs. gap), M25 (≠ V22), A66 (= A62), D67 (= D63), I68 (= I64), P136 (= P125), E137 (= E126), L140 (= L129), T290 (≠ R280), K293 (≠ S283)
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
32% identity, 93% coverage: 13:660/697 of query aligns to 27:679/719 of 6tnmA
- active site: A68 (= A62), F73 (= F67), G116 (= G103), E119 (= E106), P138 (= P125), E139 (= E126), G147 (= G134), N271 (≠ K254), S429 (= S413), H450 (= H434), E462 (= E446), N500 (= N484)
- binding adenosine-5'-triphosphate: D343 (≠ E327), I344 (≠ M328), V400 (= V384), V401 (≠ F385), V406 (= V390), K584 (= K564)
P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 93% coverage: 13:660/697 of query aligns to 27:679/729 of P21177
- G116 (= G103) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
- G322 (= G306) mutation to A: 10-fold increase in KM for NADH.
- H450 (= H434) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.
8oqqA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-79
29% identity, 97% coverage: 14:687/697 of query aligns to 20:723/723 of 8oqqA
Query Sequence
>WP_041097743.1 NCBI__GCF_000828635.1:WP_041097743.1
MSSTNYQLHGSTAVITLDNPPVNGLGYDLRCGIVAGIDQATADPAVKAVVLIGSDRAFSG
GADIREFGSPKSYAEPNLLTVIRIVEACPKPVVAAIGGVCMGGGLELTLGCHFRVAIAGA
SIALPEIKLGLLPGAGGTQRMPRIVGVEAALNLILSGNPVPSEKFKGTPLFDEFIEGNGG
DLLKGALAFADKVVAEKRPLKRIRDIRIDYPGQEAFFQFSRNMVKGVAGPFPAPFKCIDA
VQAALTKPFDEGMKFERACFIELMGTPESRALRHAFTGERAASKIPDVPDDTPARKIARV
GVIGAGTMGGGITMNFLNAGIPVTMLEMKQEALDKGIATIRKNYENSMKKGKLTQEKLDQ
RMGMLSGTLSYDDFKDADLIIEAVFEEISVKEAVFKQLDAIAKPGAILASNTSTLDVNKI
ANFTKRPQDVVGMHFFSPANVMKLLEVVRGAATAKDVMATVMQVAKKIKKTAVVSGVCDG
FIGNRMIEHYGRVAGFLLEEGATPAQVDKALEKWGMAMGPFRMGDLAGNDIGWAIRKRRY
VEKPSIRYAKFADKLCEQGRYGQKTGKGWYLYQPGNRKAIPDPEVDTMLEAYRKELGITP
RKISDAEIVQRCIYALVNEGARILEEGIAARASDIDMIYLTGYGFPLHRGGPMLYADEVG
LYNVARSMKEFAATSGDAFWEPAPLIAKRVADGKSLT
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory