SitesBLAST
Comparing WP_041098386.1 NCBI__GCF_000828635.1:WP_041098386.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3ozbA Crystal structure of 5'-methylthioinosine phosphorylase from psedomonas aeruginosa in complex with hypoxanthine (see paper)
52% identity, 96% coverage: 4:239/245 of query aligns to 6:236/241 of 3ozbA
- active site: T10 (≠ S8), P33 (= P31), F56 (≠ I57), P57 (= P58), A83 (≠ S84), M182 (= M183), T183 (= T184), N206 (= N207), A208 (= A209), M218 (≠ F221)
- binding hypoxanthine: A83 (≠ S84), V84 (= V85), G85 (= G86), L163 (= L164), E164 (= E165), V180 (= V181), G181 (= G182), M182 (= M183), N206 (= N207)
Q8U2I1 6-oxopurine nucleoside phosphorylase; Purine nucleoside phosphorylase; PNP; PfPNP; EC 2.4.2.1 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 2 papers)
45% identity, 88% coverage: 2:216/245 of query aligns to 4:220/265 of Q8U2I1
- C136 (≠ D132) modified: Disulfide link with 202
- C162 (≠ T158) modified: Disulfide link with 190
- E169 (= E165) mutation to S: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with D-211, D-213 and A-223.
- C190 (≠ M186) modified: Disulfide link with 162
- C202 (≠ P198) modified: Disulfide link with 136
- N211 (= N207) mutation to D: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-213 and A-223.
- A213 (= A209) mutation to D: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and A-223.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 223 H→A: Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and D-213.
- 254 modified: Disulfide link with 256; C→S: Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-256.
- 256 modified: Disulfide link with 254; C→S: Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-254.
9jd2A Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase from aeropyrum pernix complex with 5'-deoxy-5'-methylthioadenosine 353k
41% identity, 100% coverage: 2:245/245 of query aligns to 12:252/273 of 9jd2A
1wtaA Crystal structure of 5'-deoxy-5'-methylthioadenosine from aeropyrum pernix (r32 form)
41% identity, 100% coverage: 2:245/245 of query aligns to 12:252/273 of 1wtaA
Q8U4Q8 S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; PfMTAP; EC 2.4.2.28 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
37% identity, 96% coverage: 2:235/245 of query aligns to 4:240/257 of Q8U4Q8
- C130 (≠ D132) modified: Disulfide link with 195
- C195 (≠ P198) modified: Disulfide link with 130
Sites not aligning to the query:
- 246 modified: Disulfide link with 248
- 248 modified: Disulfide link with 246
3t94A Crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase (mtap) ii complexed with 5'-deoxy-5'-methylthioadenosine and sulfate (see paper)
37% identity, 100% coverage: 2:245/245 of query aligns to 10:249/270 of 3t94A
- active site: S16 (= S8), P39 (= P31), H63 (= H55), I65 (= I57), P66 (= P58), A92 (≠ S84), M190 (= M183), T191 (= T184), D214 (≠ N207), D216 (≠ A209), A225 (≠ F221)
- binding 5'-deoxy-5'-methylthioadenosine: G94 (= G86), F170 (≠ L164), I188 (≠ V181), M190 (= M183), D214 (≠ N207), A225 (≠ F221), V228 (≠ L224)
Q97W94 S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; MTAPII; EC 2.4.2.28 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
37% identity, 100% coverage: 2:245/245 of query aligns to 10:249/270 of Q97W94
- C138 (≠ D132) modified: Disulfide link with 205
- C200 (≠ R193) modified: Disulfide link with 262
- C205 (≠ P198) modified: Disulfide link with 138
Sites not aligning to the query:
- 259 modified: Disulfide link with 261; C→S: Reduces thermostability of the enzyme; when associated with S-261.
- 261 modified: Disulfide link with 259; C→S: Reduces thermostability of the enzyme; when associated with S-259.
- 262 modified: Disulfide link with 200; C→S: Reduces thermostability of the enzyme.
4gljA Crystal structure of methylthioadenosine phosphorylase in complex with rhodamine b (see paper)
44% identity, 84% coverage: 2:208/245 of query aligns to 6:213/287 of 4gljA
- active site: D212 (≠ N207)
- binding N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium: S12 (= S8), Y15 (≠ T11), H55 (= H51), A88 (≠ S84), G90 (= G86), F169 (≠ L164), F169 (≠ L164), I186 (≠ V181), G187 (= G182), M188 (= M183), D212 (≠ N207), F213 (≠ W208)
Sites not aligning to the query:
1sd2A Structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase complexed with 5'-methylthiotubercidin (see paper)
36% identity, 84% coverage: 2:208/245 of query aligns to 4:207/262 of 1sd2A
- active site: T10 (≠ S8), P33 (= P31), H57 (= H55), I59 (= I57), M60 (≠ P58), A86 (≠ S84), M182 (= M183), T183 (= T184), D206 (≠ N207)
- binding 2-(4-amino-pyrrolo[2,3-d]pyrimidin-7-yl)-5-methylsulfanylmethyl-tetrahydro-furan-3,4-diol: C87 (≠ V85), G88 (= G86), F163 (≠ L164), I180 (≠ V181), N181 (≠ G182), M182 (= M183), D206 (≠ N207)
Sites not aligning to the query:
5f7jB Crystal structure of mutant n87t of adenosine/methylthioadenosine phosphorylase from schistosoma mansoni in complex with adenine (see paper)
36% identity, 97% coverage: 2:239/245 of query aligns to 6:261/291 of 5f7jB
- active site: K34 (≠ E30), H59 (= H55), D230 (≠ N207), D232 (≠ A209)
- binding adenine: A88 (≠ S84), C89 (≠ V85), G90 (= G86), F187 (≠ L164), V204 (= V181), N205 (≠ G182), M206 (= M183), D230 (≠ N207), D232 (≠ A209), V246 (≠ S223)
6dz0A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-((pent-4-yn-1-ylthio)methyl)pyrrolidin-3- ol (see paper)
31% identity, 97% coverage: 2:239/245 of query aligns to 6:245/274 of 6dz0A
- active site: T12 (≠ S8), P35 (= P31), H59 (= H55), I61 (= I57), M62 (≠ P58), A88 (≠ S84), M190 (= M183), T191 (= T184), D214 (≠ N207), D216 (≠ A209), V227 (≠ F221)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(pent-4-yn-1-yl)sulfanyl]methyl}pyrrolidin-3-ol: T12 (≠ S8), H59 (= H55), A88 (≠ S84), C89 (≠ V85), G90 (= G86), F171 (≠ L164), I188 (≠ V181), N189 (≠ G182), M190 (= M183), T213 (≠ A206), D214 (≠ N207), D216 (≠ A209), V227 (≠ F221), V230 (≠ L224)
- binding phosphate ion: G11 (= G7), T12 (≠ S8), R54 (= R50), H55 (= H51), T87 (≠ A83), T191 (= T184)
5tc5A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with butylthio-dadme-immucillin-a and chloride
31% identity, 97% coverage: 2:239/245 of query aligns to 17:256/286 of 5tc5A
- active site: T23 (≠ S8), P46 (= P31), H70 (= H55), I72 (= I57), M73 (≠ P58), A99 (≠ S84), M201 (= M183), T202 (= T184), D225 (≠ N207), D227 (≠ A209), V238 (≠ F221)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(butylsulfanyl)methyl]pyrrolidin-3-ol: A99 (≠ S84), C100 (≠ V85), G101 (= G86), F182 (≠ L164), I199 (≠ V181), M201 (= M183), D225 (≠ N207), D227 (≠ A209), V241 (≠ L224)
5eubA Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with 2-amino-mta and sulfate
31% identity, 97% coverage: 2:239/245 of query aligns to 3:242/272 of 5eubA
- active site: T9 (≠ S8), P32 (= P31), H56 (= H55), I58 (= I57), M59 (≠ P58), A85 (≠ S84), M187 (= M183), T188 (= T184), D211 (≠ N207), D213 (≠ A209), V224 (≠ F221)
- binding (2~{R},3~{R},4~{S},5~{S})-2-[2,6-bis(azanyl)purin-9-yl]-5-(methylsulfanylmethyl)oxolane-3,4-diol: A85 (≠ S84), C86 (≠ V85), G87 (= G86), F168 (≠ L164), I185 (≠ V181), N186 (≠ G182), T210 (≠ A206), D211 (≠ N207), D213 (≠ A209), V227 (≠ L224)
6dz3A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-(((3-(1-butyl-1h-1,2,3-triazol-4-yl) propyl)thio)methyl)pyrrolidin-3-ol (see paper)
31% identity, 97% coverage: 2:239/245 of query aligns to 4:243/273 of 6dz3A
- active site: T10 (≠ S8), P33 (= P31), H57 (= H55), I59 (= I57), M60 (≠ P58), A86 (≠ S84), M188 (= M183), T189 (= T184), D212 (≠ N207), D214 (≠ A209), V225 (≠ F221)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-({[3-(1-butyl-1H-1,2,3-triazol-4-yl)propyl]sulfanyl}methyl)pyrrolidin-3-ol: T84 (≠ V82), A86 (≠ S84), C87 (≠ V85), G88 (= G86), F169 (≠ L164), I186 (≠ V181), N187 (≠ G182), M188 (= M183), D212 (≠ N207), D214 (≠ A209), V228 (≠ L224), L232 (= L228)
6dyzA Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with (3r,4s)-1-((4-amino-5h-pyrrolo[3,2- d]pyrimidin-7-yl)methyl)-4-((prop-2-yn-1-ylthio)methyl)pyrrolidin-3- ol (see paper)
31% identity, 97% coverage: 2:239/245 of query aligns to 4:243/273 of 6dyzA
- active site: T10 (≠ S8), P33 (= P31), H57 (= H55), I59 (= I57), M60 (≠ P58), A86 (≠ S84), M188 (= M183), T189 (= T184), D212 (≠ N207), D214 (≠ A209), V225 (≠ F221)
- binding (3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-{[(prop-2-yn-1-yl)sulfanyl]methyl}pyrrolidin-3-ol: T10 (≠ S8), H57 (= H55), A86 (≠ S84), C87 (≠ V85), G88 (= G86), F169 (≠ L164), I186 (≠ V181), N187 (≠ G182), M188 (= M183), D212 (≠ N207), D214 (≠ A209), V228 (≠ L224)
- binding phosphate ion: G9 (= G7), T10 (≠ S8), R52 (= R50), H53 (= H51), T85 (≠ A83), A86 (≠ S84), T189 (= T184)
5tc8A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with methylthio-dadme-immucillin-a
31% identity, 97% coverage: 2:239/245 of query aligns to 4:243/273 of 5tc8A
- active site: T10 (≠ S8), P33 (= P31), H57 (= H55), I59 (= I57), M60 (≠ P58), A86 (≠ S84), M188 (= M183), T189 (= T184), D212 (≠ N207), D214 (≠ A209), V225 (≠ F221)
- binding (3r,4s)-1-[(4-amino-5h-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-4-[(methylsulfanyl)methyl]pyrrolidin-3-ol: T10 (≠ S8), A86 (≠ S84), C87 (≠ V85), G88 (= G86), F169 (≠ L164), I186 (≠ V181), N187 (≠ G182), M188 (= M183), T211 (≠ A206), D212 (≠ N207), D214 (≠ A209), V228 (≠ L224)
5tc6A Crystal structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase in complex with propylthio-immucillin-a
31% identity, 97% coverage: 2:239/245 of query aligns to 4:243/273 of 5tc6A
- active site: T10 (≠ S8), P33 (= P31), H57 (= H55), I59 (= I57), M60 (≠ P58), A86 (≠ S84), M188 (= M183), T189 (= T184), D212 (≠ N207), D214 (≠ A209), V225 (≠ F221)
- binding (2S,3S,4R,5S)-2-(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)-5-[(propylsulfanyl)methyl]pyrrolidine-3,4-diol: T10 (≠ S8), A86 (≠ S84), C87 (≠ V85), G88 (= G86), F169 (≠ L164), N187 (≠ G182), M188 (= M183), D212 (≠ N207), V225 (≠ F221), V228 (≠ L224), L229 (≠ E225)
3ozcA Crystal structure of human 5'-deoxy-5'-methyladenosine phosphorylase in complex with pcl-phenylthiodadmeimma
31% identity, 97% coverage: 2:239/245 of query aligns to 4:243/273 of 3ozcA