SitesBLAST
Comparing WP_041098567.1 NCBI__GCF_000828635.1:WP_041098567.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
36% identity, 92% coverage: 22:263/264 of query aligns to 17:258/259 of 5zaiC
- active site: A65 (≠ H70), F70 (≠ M75), S82 (≠ F88), R86 (≠ G92), G110 (≠ A116), E113 (≠ Q119), P132 (≠ S138), E133 (≠ G139), I138 (≠ L144), P140 (≠ C146), G141 (≠ S147), A226 (= A233), F236 (vs. gap)
- binding coenzyme a: K24 (≠ Q29), L25 (≠ F30), A63 (= A68), G64 (= G69), A65 (≠ H70), D66 (= D71), I67 (≠ L72), P132 (≠ S138), R166 (≠ F171), F248 (= F253), K251 (= K256)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 97% coverage: 7:261/264 of query aligns to 1:249/250 of 3q0gD
- active site: A64 (≠ H70), M69 (= M75), T75 (≠ M84), F79 (= F88), G103 (≠ A116), E106 (≠ Q119), P125 (≠ S138), E126 (≠ G139), V131 (≠ L144), P133 (≠ C146), G134 (≠ S147), L219 (≠ Q231), F229 (≠ M241)
- binding Butyryl Coenzyme A: F225 (≠ M237), F241 (= F253)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
35% identity, 95% coverage: 13:262/264 of query aligns to 11:258/260 of 2hw5C
- active site: A68 (≠ H70), M73 (= M75), S83 (≠ M84), L87 (≠ F88), G111 (≠ A116), E114 (≠ Q119), P133 (≠ S138), E134 (≠ G139), T139 (≠ L144), P141 (≠ C146), G142 (≠ S147), K227 (≠ Q231), F237 (≠ M241)
- binding crotonyl coenzyme a: K26 (≠ T28), A27 (≠ Q29), L28 (≠ F30), A30 (≠ S32), K62 (= K64), I70 (≠ L72), F109 (≠ T114)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
33% identity, 97% coverage: 7:261/264 of query aligns to 2:254/255 of 3q0jC
- active site: A65 (≠ H70), M70 (= M75), T80 (≠ M84), F84 (= F88), G108 (≠ A116), E111 (≠ Q119), P130 (≠ S138), E131 (≠ G139), V136 (≠ L144), P138 (≠ C146), G139 (≠ S147), L224 (≠ Q231), F234 (≠ M241)
- binding acetoacetyl-coenzyme a: Q23 (≠ T28), A24 (≠ Q29), L25 (≠ F30), A27 (≠ S32), A63 (= A68), G64 (= G69), A65 (≠ H70), D66 (= D71), I67 (≠ L72), K68 (= K73), M70 (= M75), F84 (= F88), G107 (≠ A115), G108 (≠ A116), E111 (≠ Q119), P130 (≠ S138), E131 (≠ G139), P138 (≠ C146), G139 (≠ S147), M140 (≠ T148)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 97% coverage: 7:261/264 of query aligns to 2:254/255 of 3q0gC
- active site: A65 (≠ H70), M70 (= M75), T80 (≠ M84), F84 (= F88), G108 (≠ A116), E111 (≠ Q119), P130 (≠ S138), E131 (≠ G139), V136 (≠ L144), P138 (≠ C146), G139 (≠ S147), L224 (≠ Q231), F234 (≠ M241)
- binding coenzyme a: L25 (≠ F30), A63 (= A68), I67 (≠ L72), K68 (= K73), Y104 (≠ I112), P130 (≠ S138), E131 (≠ G139), L134 (≠ V142)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
33% identity, 97% coverage: 7:261/264 of query aligns to 1:253/256 of 3h81A
- active site: A64 (≠ H70), M69 (= M75), T79 (≠ M84), F83 (= F88), G107 (≠ A116), E110 (≠ Q119), P129 (≠ S138), E130 (≠ G139), V135 (≠ L144), P137 (≠ C146), G138 (≠ S147), L223 (≠ Q231), F233 (≠ M241)
- binding calcium ion: F233 (≠ M241), Q238 (≠ A246)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
35% identity, 92% coverage: 19:262/264 of query aligns to 16:252/254 of 2dubA
- active site: A67 (≠ H70), M72 (= M75), S82 (≠ K93), G105 (≠ A116), E108 (≠ Q119), P127 (≠ S138), E128 (≠ G139), T133 (≠ L144), P135 (≠ C146), G136 (≠ S147), K221 (≠ Q231), F231 (≠ M241)
- binding octanoyl-coenzyme a: K25 (≠ T28), A26 (≠ Q29), L27 (≠ F30), A29 (≠ S32), A65 (= A68), A67 (≠ H70), D68 (= D71), I69 (≠ L72), K70 (= K73), G105 (≠ A116), E108 (≠ Q119), P127 (≠ S138), E128 (≠ G139), G136 (≠ S147), A137 (≠ T148)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
35% identity, 92% coverage: 19:262/264 of query aligns to 17:258/260 of 1dubA
- active site: A68 (≠ H70), M73 (= M75), S83 (vs. gap), L87 (≠ M95), G111 (≠ A116), E114 (≠ Q119), P133 (≠ S138), E134 (≠ G139), T139 (≠ L144), P141 (≠ C146), G142 (≠ S147), K227 (≠ Q231), F237 (≠ M241)
- binding acetoacetyl-coenzyme a: K26 (≠ T28), A27 (≠ Q29), L28 (≠ F30), A30 (≠ S32), A66 (= A68), A68 (≠ H70), D69 (= D71), I70 (≠ L72), Y107 (≠ I112), G110 (≠ A115), G111 (≠ A116), E114 (≠ Q119), P133 (≠ S138), E134 (≠ G139), L137 (≠ V142), G142 (≠ S147), F233 (≠ M237), F249 (= F253)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
35% identity, 92% coverage: 19:262/264 of query aligns to 47:288/290 of P14604
- E144 (≠ Q119) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ G139) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
35% identity, 92% coverage: 19:262/264 of query aligns to 15:256/258 of 1ey3A
- active site: A66 (≠ H70), M71 (= M75), S81 (vs. gap), L85 (≠ M95), G109 (≠ A116), E112 (≠ Q119), P131 (≠ S138), E132 (≠ G139), T137 (≠ L144), P139 (≠ C146), G140 (≠ S147), K225 (≠ Q231), F235 (≠ M241)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ T28), L26 (≠ F30), A28 (≠ S32), A64 (= A68), G65 (= G69), A66 (≠ H70), D67 (= D71), I68 (≠ L72), L85 (≠ M95), W88 (vs. gap), G109 (≠ A116), P131 (≠ S138), L135 (≠ V142), G140 (≠ S147)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
34% identity, 92% coverage: 19:262/264 of query aligns to 17:256/258 of 1mj3A
- active site: A68 (≠ H70), M73 (= M75), S83 (≠ K93), L85 (≠ M95), G109 (≠ A116), E112 (≠ Q119), P131 (≠ S138), E132 (≠ G139), T137 (≠ L144), P139 (≠ C146), G140 (≠ S147), K225 (≠ Q231), F235 (≠ M241)
- binding hexanoyl-coenzyme a: K26 (≠ T28), A27 (≠ Q29), L28 (≠ F30), A30 (≠ S32), A66 (= A68), G67 (= G69), A68 (≠ H70), D69 (= D71), I70 (≠ L72), G109 (≠ A116), P131 (≠ S138), E132 (≠ G139), L135 (≠ V142), G140 (≠ S147)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
32% identity, 96% coverage: 10:263/264 of query aligns to 4:256/257 of 6slbAAA
- active site: Q64 (≠ H70), F69 (≠ M75), L80 (≠ F88), N84 (≠ G92), A108 (= A116), S111 (≠ Q119), A130 (≠ S138), F131 (≠ G139), L136 (= L144), P138 (≠ C146), D139 (vs. gap), A224 (≠ Q231), G234 (≠ M241)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K64), A62 (= A68), Q64 (≠ H70), D65 (= D71), L66 (= L72), Y76 (≠ M84), A108 (= A116), F131 (≠ G139), D139 (vs. gap)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
32% identity, 96% coverage: 10:263/264 of query aligns to 1:244/245 of 6slaAAA
- active site: Q61 (≠ H70), L68 (≠ S80), N72 (≠ G92), A96 (= A116), S99 (≠ Q119), A118 (≠ S138), F119 (≠ G139), L124 (= L144), P126 (≠ C146), N127 (≠ S147), A212 (≠ Q231), G222 (≠ M241)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ F30), A59 (= A68), Q61 (≠ H70), D62 (= D71), L63 (= L72), L68 (≠ S80), Y71 (= Y83), A94 (≠ T114), G95 (≠ A115), A96 (= A116), F119 (≠ G139), I122 (≠ V142), L124 (= L144), N127 (≠ S147), F234 (= F253), K237 (= K256)
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 93% coverage: 17:261/264 of query aligns to 85:330/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
32% identity, 97% coverage: 8:263/264 of query aligns to 4:260/261 of 5jbxB
- active site: A67 (≠ H70), R72 (= R76), L84 (≠ F88), R88 (≠ G92), G112 (≠ A116), E115 (≠ Q119), T134 (≠ S138), E135 (≠ G139), I140 (≠ L144), P142 (≠ C146), G143 (≠ S147), A228 (≠ Q231), L238 (≠ M241)
- binding coenzyme a: S24 (≠ T28), R25 (≠ Q29), R26 (≠ F30), A28 (≠ S32), A65 (= A68), D68 (= D71), L69 (= L72), K70 (= K73), L110 (≠ T114), G111 (≠ A115), T134 (≠ S138), E135 (≠ G139), L138 (≠ V142), R168 (≠ F171)
Q5HH38 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Staphylococcus aureus (strain COL) (see paper)
33% identity, 93% coverage: 17:261/264 of query aligns to 21:266/273 of Q5HH38
- R34 (≠ F30) binding in other chain
- SGGD--Q 73:77 (≠ AGHDLKQ 68:74) binding in other chain
- S149 (≠ L144) binding in other chain
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
30% identity, 91% coverage: 22:262/264 of query aligns to 24:266/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
2uzfA Crystal structure of staphylococcus aureus 1,4-dihydroxy-2-naphthoyl coa synthase (menb) in complex with acetoacetyl coa (see paper)
33% identity, 93% coverage: 17:261/264 of query aligns to 16:253/260 of 2uzfA
- active site: G70 (≠ H70), R80 (≠ K86), L84 (≠ Q90), G108 (≠ A116), V111 (≠ Q119), T130 (≠ S138), G131 (= G139), S136 (≠ L144), D138 (vs. gap), A139 (≠ C146), A225 (= A233), Y233 (≠ M241)
- binding acetoacetyl-coenzyme a: V28 (≠ Q29), R29 (≠ F30), S68 (≠ A68), G69 (= G69), G70 (≠ H70), D71 (= D71), Y104 (≠ I112), G108 (≠ A116)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
30% identity, 96% coverage: 9:262/264 of query aligns to 8:264/266 of O53561
- K135 (≠ R134) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 134:141, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ N141) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
4emlA Synechocystis sp. Pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase (menb) in complex with bicarbonate (see paper)
32% identity, 92% coverage: 18:261/264 of query aligns to 19:254/261 of 4emlA
- active site: G77 (≠ H70), R81 (= R76), L85 (≠ S80), G109 (≠ A116), V112 (≠ Q119), G132 (= G139), S137 (≠ L144), D139 (vs. gap), G140 (≠ C146), A226 (= A233), Y234 (≠ M241)
- binding bicarbonate ion: G108 (≠ A115), Q130 (≠ V137), G132 (= G139), W160 (≠ L166)
- binding chloride ion: D184 (≠ E190), R185 (≠ Q191), E187 (≠ D193), E188 (≠ A194)
Query Sequence
>WP_041098567.1 NCBI__GCF_000828635.1:WP_041098567.1
MNAPETTLPILMREDREGVATLTLNRPTQFNSLSEEMLGELQAALNAIAADKTVRAVVIA
GAGKAFCAGHDLKQMRANHSKTYMQKLFKQCGKVMMSIVEMPQPVIARIHGIATAAGCQL
VAMCDLAVAAEGARFAVSGINVGLFCSTPSVALGRAMGRKEAMEMLLTGDFIDAAEAQRR
GLINRVVPLEQLDAEVKKLTDSICAKSAVAVGMGKQMFYKQLEMGLDGAYQLAVETMACN
MMADDAAEGIDAFMQKRKPEWKGC
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory