SitesBLAST
Comparing WP_041099045.1 NCBI__GCF_000828635.1:WP_041099045.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
40% identity, 95% coverage: 15:268/268 of query aligns to 12:257/257 of 6slbAAA
- active site: Q64 (≠ G67), F69 (= F72), L80 (≠ I91), N84 (≠ H95), A108 (≠ F119), S111 (= S122), A130 (= A141), F131 (≠ Y142), L136 (≠ T147), P138 (= P149), D139 (= D150), A224 (≠ Q235), G234 (≠ A245)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ E61), A62 (= A65), Q64 (≠ G67), D65 (= D68), L66 (≠ I69), Y76 (≠ L87), A108 (≠ F119), F131 (≠ Y142), D139 (= D150)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
39% identity, 95% coverage: 15:268/268 of query aligns to 9:245/245 of 6slaAAA
- active site: Q61 (≠ G67), L68 (≠ I91), N72 (≠ H95), A96 (≠ F119), S99 (= S122), A118 (= A141), F119 (≠ Y142), L124 (≠ T147), P126 (= P149), N127 (≠ D150), A212 (≠ Q235), G222 (≠ A245)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L27), A59 (= A65), Q61 (≠ G67), D62 (= D68), L63 (≠ I69), L68 (≠ I91), Y71 (≠ V94), A94 (= A117), G95 (= G118), A96 (≠ F119), F119 (≠ Y142), I122 (= I145), L124 (≠ T147), N127 (≠ D150), F234 (= F257), K237 (= K260)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
33% identity, 97% coverage: 8:268/268 of query aligns to 6:259/259 of 5zaiC
- active site: A65 (≠ G67), F70 (= F72), S82 (≠ I91), R86 (≠ H95), G110 (≠ F119), E113 (≠ S122), P132 (≠ A141), E133 (≠ Y142), I138 (≠ T147), P140 (= P149), G141 (≠ D150), A226 (≠ Q235), F236 (≠ A245)
- binding coenzyme a: K24 (≠ A26), L25 (= L27), A63 (= A65), G64 (= G66), A65 (≠ G67), D66 (= D68), I67 (= I69), P132 (≠ A141), R166 (= R175), F248 (= F257), K251 (= K260)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
33% identity, 99% coverage: 1:265/268 of query aligns to 1:254/255 of 3q0jC
- active site: A65 (≠ G67), M70 (≠ F72), T80 (≠ A80), F84 (≠ R84), G108 (≠ F119), E111 (≠ S122), P130 (≠ A141), E131 (≠ Y142), V136 (≠ T147), P138 (= P149), G139 (≠ D150), L224 (≠ Q235), F234 (≠ A245)
- binding acetoacetyl-coenzyme a: Q23 (≠ K25), A24 (= A26), L25 (= L27), A27 (= A29), A63 (= A65), G64 (= G66), A65 (≠ G67), D66 (= D68), I67 (= I69), K68 (= K70), M70 (≠ F72), F84 (≠ R84), G107 (= G118), G108 (≠ F119), E111 (≠ S122), P130 (≠ A141), E131 (≠ Y142), P138 (= P149), G139 (≠ D150), M140 (≠ G151)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 99% coverage: 1:265/268 of query aligns to 1:254/255 of 3q0gC
- active site: A65 (≠ G67), M70 (≠ F72), T80 (≠ A80), F84 (≠ R84), G108 (≠ F119), E111 (≠ S122), P130 (≠ A141), E131 (≠ Y142), V136 (≠ T147), P138 (= P149), G139 (≠ D150), L224 (≠ Q235), F234 (≠ A245)
- binding coenzyme a: L25 (= L27), A63 (= A65), I67 (= I69), K68 (= K70), Y104 (≠ A115), P130 (≠ A141), E131 (≠ Y142), L134 (≠ I145)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 97% coverage: 6:265/268 of query aligns to 5:249/250 of 3q0gD
- active site: A64 (≠ G67), M69 (≠ F72), T75 (≠ A80), F79 (≠ R84), G103 (≠ F119), E106 (≠ S122), P125 (≠ A141), E126 (≠ Y142), V131 (≠ T147), P133 (= P149), G134 (≠ D150), L219 (≠ Q235), F229 (≠ A245)
- binding Butyryl Coenzyme A: F225 (= F241), F241 (= F257)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
33% identity, 97% coverage: 6:265/268 of query aligns to 5:253/256 of 3h81A
- active site: A64 (≠ G67), M69 (≠ F72), T79 (≠ A80), F83 (≠ R84), G107 (≠ F119), E110 (≠ S122), P129 (≠ A141), E130 (≠ Y142), V135 (≠ T147), P137 (= P149), G138 (≠ D150), L223 (≠ Q235), F233 (≠ A245)
- binding calcium ion: F233 (≠ A245), Q238 (≠ F250)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
32% identity, 94% coverage: 14:265/268 of query aligns to 15:257/260 of 1dubA
- active site: A68 (≠ G67), M73 (≠ F72), S83 (≠ I91), L87 (≠ H95), G111 (≠ F119), E114 (≠ S122), P133 (≠ A141), E134 (≠ Y142), T139 (= T147), P141 (= P149), G142 (≠ D150), K227 (≠ Q235), F237 (≠ A245)
- binding acetoacetyl-coenzyme a: K26 (= K25), A27 (= A26), L28 (= L27), A30 (= A29), A66 (= A65), A68 (≠ G67), D69 (= D68), I70 (= I69), Y107 (≠ A115), G110 (= G118), G111 (≠ F119), E114 (≠ S122), P133 (≠ A141), E134 (≠ Y142), L137 (≠ I145), G142 (≠ D150), F233 (= F241), F249 (= F257)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
32% identity, 94% coverage: 14:265/268 of query aligns to 13:255/258 of 1ey3A
- active site: A66 (≠ G67), M71 (≠ F72), S81 (≠ I91), L85 (≠ H95), G109 (≠ F119), E112 (≠ S122), P131 (≠ A141), E132 (≠ Y142), T137 (= T147), P139 (= P149), G140 (≠ D150), K225 (≠ Q235), F235 (≠ A245)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K25), L26 (= L27), A28 (= A29), A64 (= A65), G65 (= G66), A66 (≠ G67), D67 (= D68), I68 (= I69), L85 (≠ H95), W88 (≠ V98), G109 (≠ F119), P131 (≠ A141), L135 (≠ I145), G140 (≠ D150)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
32% identity, 94% coverage: 14:265/268 of query aligns to 45:287/290 of P14604
- E144 (≠ S122) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ Y142) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
32% identity, 94% coverage: 14:265/268 of query aligns to 14:251/254 of 2dubA
- active site: A67 (≠ G67), M72 (≠ F72), S82 (≠ E96), G105 (≠ F119), E108 (≠ S122), P127 (≠ A141), E128 (≠ Y142), T133 (= T147), P135 (= P149), G136 (≠ D150), K221 (≠ Q235), F231 (≠ A245)
- binding octanoyl-coenzyme a: K25 (= K25), A26 (= A26), L27 (= L27), A29 (= A29), A65 (= A65), A67 (≠ G67), D68 (= D68), I69 (= I69), K70 (= K70), G105 (≠ F119), E108 (≠ S122), P127 (≠ A141), E128 (≠ Y142), G136 (≠ D150), A137 (≠ G151)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
33% identity, 94% coverage: 14:265/268 of query aligns to 15:255/258 of 1mj3A
- active site: A68 (≠ G67), M73 (≠ F72), S83 (≠ I91), L85 (≠ E93), G109 (≠ F119), E112 (≠ S122), P131 (≠ A141), E132 (≠ Y142), T137 (= T147), P139 (= P149), G140 (≠ D150), K225 (≠ Q235), F235 (≠ A245)
- binding hexanoyl-coenzyme a: K26 (= K25), A27 (= A26), L28 (= L27), A30 (= A29), A66 (= A65), G67 (= G66), A68 (≠ G67), D69 (= D68), I70 (= I69), G109 (≠ F119), P131 (≠ A141), E132 (≠ Y142), L135 (≠ I145), G140 (≠ D150)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
32% identity, 93% coverage: 16:265/268 of query aligns to 17:257/260 of 2hw5C
- active site: A68 (≠ G67), M73 (≠ F72), S83 (≠ R83), L87 (= L87), G111 (≠ F119), E114 (≠ S122), P133 (≠ A141), E134 (≠ Y142), T139 (= T147), P141 (= P149), G142 (≠ D150), K227 (≠ Q235), F237 (≠ A245)
- binding crotonyl coenzyme a: K26 (= K25), A27 (= A26), L28 (= L27), A30 (= A29), K62 (≠ E61), I70 (= I69), F109 (≠ A117)
3t3wF Crystal structure of probable enoyl-coa hydratase from mycobacterium thermoresistibile (see paper)
36% identity, 83% coverage: 6:227/268 of query aligns to 4:217/248 of 3t3wF
- active site: H65 (≠ G67), D71 (≠ H73), S83 (≠ R83), L87 (= L87), G111 (≠ F119), L114 (≠ S122), V134 (≠ L140), I139 (= I145), G140 (= G146), E143 (≠ P149)
- binding zinc ion: E82 (= E82), E143 (≠ P149)
Sites not aligning to the query:
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
33% identity, 97% coverage: 8:268/268 of query aligns to 7:261/261 of 5jbxB
- active site: A67 (≠ G67), R72 (≠ T75), L84 (= L87), R88 (≠ H95), G112 (≠ F119), E115 (≠ S122), T134 (≠ A141), E135 (≠ Y142), I140 (≠ T147), P142 (= P149), G143 (≠ D150), A228 (≠ Q235), L238 (≠ A245)
- binding coenzyme a: S24 (≠ K25), R25 (≠ A26), R26 (≠ L27), A28 (= A29), A65 (= A65), D68 (= D68), L69 (≠ I69), K70 (= K70), L110 (≠ A117), G111 (= G118), T134 (≠ A141), E135 (≠ Y142), L138 (≠ I145), R168 (= R175)
3omeC Crystal structure of a probable enoyl-coa hydratase from mycobacterium smegmatis (see paper)
34% identity, 84% coverage: 3:227/268 of query aligns to 1:216/247 of 3omeC
- active site: H65 (≠ G67), E70 (≠ R83), A82 (= A97), L86 (vs. gap), G110 (≠ F119), L113 (≠ S122), V133 (≠ L140), I138 (= I145), G139 (= G146), E142 (≠ P149)
- binding zinc ion: E81 (= E96), E142 (≠ P149)
Sites not aligning to the query:
6l3pA Crystal strcuture of feruloyl-coa hydratase lyase(fchl) complexed with coa
33% identity, 79% coverage: 3:215/268 of query aligns to 5:210/244 of 6l3pA
- active site: M69 (≠ G67), Y74 (≠ F72), R86 (≠ G92), Q90 (≠ E96), G114 (≠ F119), S117 (= S122), S136 (≠ A141), E137 (≠ Y142), I142 (≠ T147), P144 (= P149), G145 (≠ D150)
- binding coenzyme a: K28 (≠ A26), R29 (≠ L27), A31 (= A29), A67 (= A65), M69 (≠ G67), D70 (= D68), L71 (≠ I69), G113 (= G118)
Sites not aligning to the query:
Q62651 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; EC 5.3.3.- from Rattus norvegicus (Rat) (see paper)
28% identity, 95% coverage: 10:264/268 of query aligns to 60:320/327 of Q62651
- D176 (≠ S122) mutation D->A,D: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- E196 (≠ Y142) mutation E->D,Q: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- D204 (= D150) mutation D->A,N: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
6p5uE Structure of an enoyl-coa hydratase/aldolase isolated from a lignin- degrading consortium (see paper)
36% identity, 74% coverage: 15:213/268 of query aligns to 15:212/246 of 6p5uE
- active site: M67 (≠ G67), Y72 (vs. gap), D77 (≠ L76), R89 (= R89), A93 (≠ E93), G117 (≠ F119), T120 (≠ S122), E140 (≠ Y142), I145 (≠ T147), P147 (= P149), A148 (≠ D150)
- binding coenzyme a: D25 (≠ K25), K26 (≠ A26), R27 (≠ L27), A29 (= A29), A65 (= A65), M67 (≠ G67), D68 (= D68), L69 (≠ I69), W113 (≠ A115), F115 (≠ A117), S139 (≠ A141), W143 (≠ I145)
Sites not aligning to the query:
4elxA Structure of apo e.Coli. 1,4-dihydroxy-2- naphthoyl coa synthases with cl (see paper)
31% identity, 95% coverage: 12:265/268 of query aligns to 27:261/268 of 4elxA
- active site: G83 (= G67), H88 (≠ F72), L92 (= L76), G116 (≠ F119), V119 (≠ S122), G139 (≠ Y142), S144 (≠ T147), D146 (≠ P149), G147 (≠ D150), A233 (= A236), Y241 (≠ A245)
- binding chloride ion: G115 (= G118), G139 (≠ Y142), W167 (≠ A170)
Query Sequence
>WP_041099045.1 NCBI__GCF_000828635.1:WP_041099045.1
MNGDGVLLETDAASGIATITLNRPKALNALDMAMADGLRATTRHVAGDATVRAVVIQGAG
EHFMAGGDIKAFHETLDAPAAERRALLERLIGEVHEAVTSIRTMEKPVIASVRGAVAGFG
FSLMSACDLAIAADSSYFTLAYRHIGTSPDGGSTYALPRLVGAKQAMEIALLGERFNAAR
ALELGLVNRVVPAAELAAATQALALELANGPTIALGRTKLLINESLNRKLAEQLQAEQDS
FAACAMTQDFARGVRGFVAKQKPRFLGN
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory