SitesBLAST
Comparing WP_041099069.1 NCBI__GCF_000828635.1:WP_041099069.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 18 hits to proteins with known functional sites (download)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
38% identity, 99% coverage: 5:399/400 of query aligns to 3:396/408 of P38502
- N7 (= N9) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S12) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (= S14) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K16) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R100) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V102) mutation to A: Decreases affinity for acetate.
- L122 (= L131) mutation to A: Decreases affinity for acetate.
- D148 (= D157) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F187) mutation to A: Decreases affinity for acetate.
- N211 (= N219) mutation to A: Slightly reduced enzyme activity.
- P232 (≠ A240) mutation to A: Decreases affinity for acetate.
- R241 (= R249) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E387) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuA Acetate kinase crystallized with atpgs (see paper)
38% identity, 99% coverage: 5:399/400 of query aligns to 3:396/399 of 1tuuA
- active site: N7 (= N9), R91 (= R100), H180 (= H188), R241 (= R249), E384 (= E387)
- binding adenosine-5'-diphosphate: K14 (= K16), G210 (= G218), D283 (= D291), F284 (≠ M292), R285 (= R293), G331 (= G336), I332 (= I337), N335 (≠ H340)
- binding sulfate ion: R91 (= R100), H180 (= H188), G212 (= G220)
1tuuB Acetate kinase crystallized with atpgs (see paper)
38% identity, 99% coverage: 5:399/400 of query aligns to 3:396/398 of 1tuuB
- active site: N7 (= N9), R91 (= R100), H180 (= H188), R241 (= R249), E384 (= E387)
- binding adenosine monophosphate: D283 (= D291), R285 (= R293), G331 (= G336), I332 (= I337), N335 (≠ H340), S336 (≠ G341)
- binding trihydrogen thiodiphosphate: H180 (= H188), G212 (= G220), R241 (= R249)
7fj9A Kpacka (pduw) with amppnp complex structure
42% identity, 99% coverage: 5:398/400 of query aligns to 4:391/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
42% identity, 99% coverage: 5:398/400 of query aligns to 4:391/395 of 7fj8A
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
37% identity, 97% coverage: 5:392/400 of query aligns to 4:383/394 of 4fwsA
- active site: N8 (= N9), R83 (= R100), H172 (= H188), R233 (= R249), E378 (= E387)
- binding cytidine-5'-triphosphate: G202 (= G218), N203 (= N219), G204 (= G220), D275 (= D291), L276 (≠ M292), R277 (= R293), G323 (= G336), I324 (= I337), N327 (≠ H340)
- binding 1,2-ethanediol: V21 (≠ Y22), C24 (≠ A25), H115 (= H132), N203 (= N219), T232 (≠ S248), R233 (= R249), K262 (= K278)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
37% identity, 97% coverage: 5:392/400 of query aligns to 4:383/394 of 4fwrA
- active site: N8 (= N9), R83 (= R100), H172 (= H188), R233 (= R249), E378 (= E387)
- binding cytidine-5'-monophosphate: G202 (= G218), N203 (= N219), D275 (= D291), L276 (≠ M292), R277 (= R293), G323 (= G336), I324 (= I337), N327 (≠ H340)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
37% identity, 97% coverage: 5:392/400 of query aligns to 4:383/394 of 4fwqA
- active site: N8 (= N9), R83 (= R100), H172 (= H188), R233 (= R249), E378 (= E387)
- binding guanosine-5'-triphosphate: H172 (= H188), N203 (= N219), G204 (= G220), D275 (= D291), L276 (≠ M292), R277 (= R293), E280 (≠ L296), G323 (= G336), I324 (= I337), N327 (≠ H340)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
37% identity, 97% coverage: 5:392/400 of query aligns to 4:383/394 of 4fwpA
- active site: N8 (= N9), R83 (= R100), H172 (= H188), R233 (= R249), E378 (= E387)
- binding 1,2-ethanediol: S11 (= S12), H115 (= H132), K262 (= K278)
- binding guanosine-5'-diphosphate: N203 (= N219), D275 (= D291), L276 (≠ M292), R277 (= R293), E280 (≠ L296), G323 (= G336), I324 (= I337), N327 (≠ H340), S328 (≠ G341)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
37% identity, 97% coverage: 5:392/400 of query aligns to 4:383/394 of 4fwoA
- active site: N8 (= N9), R83 (= R100), H172 (= H188), R233 (= R249), E378 (= E387)
- binding guanosine-5'-monophosphate: G202 (= G218), N203 (= N219), D275 (= D291), L276 (≠ M292), R277 (= R293), E280 (≠ L296), G323 (= G336), I324 (= I337), N327 (≠ H340)
- binding 1,2-ethanediol: E100 (≠ A117), N104 (≠ A121)
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
37% identity, 97% coverage: 5:392/400 of query aligns to 4:383/394 of 4fwnA
- active site: N8 (= N9), R83 (= R100), H172 (= H188), R233 (= R249), E378 (= E387)
- binding adenosine-5'-tetraphosphate: H172 (= H188), H200 (= H216), N203 (= N219), G204 (= G220), D275 (= D291), L276 (≠ M292), R277 (= R293), G323 (= G336), I324 (= I337), N327 (≠ H340)
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
37% identity, 97% coverage: 5:392/400 of query aligns to 4:383/394 of 4fwmA