SitesBLAST
Comparing WP_041378948.1 NCBI__GCF_000016765.1:WP_041378948.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
43% identity, 86% coverage: 7:222/252 of query aligns to 23:235/378 of P69874
- C26 (≠ H10) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (≠ Y11) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (= F29) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ A38) mutation to T: Loss of ATPase activity and transport.
- L60 (= L44) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ I60) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V121) mutation to M: Loss of ATPase activity and transport.
- D172 (= D159) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
42% identity, 86% coverage: 7:222/252 of query aligns to 8:220/358 of 8y5iA
7ahhC Opua inhibited inward-facing, sbd docked (see paper)
43% identity, 78% coverage: 19:214/252 of query aligns to 44:242/382 of 7ahhC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
- binding phosphoaminophosphonic acid-adenylate ester: 12, 39, 40, 41
7aheC Opua inhibited inward facing (see paper)
43% identity, 78% coverage: 19:214/252 of query aligns to 44:242/382 of 7aheC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
7ahdC Opua (e190q) occluded (see paper)
42% identity, 78% coverage: 19:214/252 of query aligns to 44:242/260 of 7ahdC
- binding adenosine-5'-triphosphate: S61 (= S36), G62 (= G37), G64 (= G39), K65 (= K40), S66 (≠ T41), T67 (= T42), Q111 (= Q82), K161 (≠ A133), Q162 (= Q134), S164 (= S136), G166 (= G138), M167 (≠ Q139), Q188 (≠ E160), H221 (= H193)
Sites not aligning to the query:
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
40% identity, 93% coverage: 1:235/252 of query aligns to 2:236/241 of 4u00A
3c4jA Abc protein artp in complex with atp-gamma-s
39% identity, 94% coverage: 1:236/252 of query aligns to 3:239/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
39% identity, 94% coverage: 1:236/252 of query aligns to 3:239/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
39% identity, 94% coverage: 1:236/252 of query aligns to 3:239/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
39% identity, 94% coverage: 1:236/252 of query aligns to 3:239/242 of 2oljA
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
39% identity, 90% coverage: 2:228/252 of query aligns to 4:227/371 of P68187
- A85 (≠ G85) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P106) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A111) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (= V114) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E116) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A125) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G138) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D159) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
39% identity, 90% coverage: 2:228/252 of query aligns to 3:226/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y11), S37 (= S36), G38 (= G37), C39 (≠ A38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), Q81 (= Q82), R128 (= R130), A132 (≠ Q134), S134 (= S136), G136 (= G138), Q137 (= Q139), E158 (= E160), H191 (= H193)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q82)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
39% identity, 90% coverage: 2:228/252 of query aligns to 3:226/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), G38 (= G37), C39 (≠ A38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), R128 (= R130), S134 (= S136), Q137 (= Q139)
- binding beryllium trifluoride ion: S37 (= S36), G38 (= G37), K41 (= K40), Q81 (= Q82), S134 (= S136), G136 (= G138), H191 (= H193)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q82)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
39% identity, 90% coverage: 2:228/252 of query aligns to 3:226/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (= V16), G38 (= G37), C39 (≠ A38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), R128 (= R130), A132 (≠ Q134), S134 (= S136), Q137 (= Q139)
- binding tetrafluoroaluminate ion: S37 (= S36), G38 (= G37), K41 (= K40), Q81 (= Q82), S134 (= S136), G135 (= G137), G136 (= G138), E158 (= E160), H191 (= H193)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q82)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
39% identity, 90% coverage: 2:228/252 of query aligns to 3:226/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (= V16), G38 (= G37), C39 (≠ A38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), R128 (= R130), A132 (≠ Q134), S134 (= S136), Q137 (= Q139)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q82)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
39% identity, 90% coverage: 2:228/252 of query aligns to 3:226/374 of 2awnB
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
39% identity, 90% coverage: 2:228/252 of query aligns to 1:224/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ Y11), S35 (= S36), G36 (= G37), C37 (≠ A38), G38 (= G39), K39 (= K40), S40 (≠ T41), T41 (= T42), R126 (= R130), A130 (≠ Q134), S132 (= S136), G134 (= G138), Q135 (= Q139)
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
36% identity, 94% coverage: 1:236/252 of query aligns to 1:237/240 of 4ymuJ
- binding adenosine-5'-triphosphate: F11 (≠ Y11), V16 (= V16), S36 (= S36), G37 (= G37), S38 (≠ A38), G39 (= G39), K40 (= K40), S41 (≠ T41), T42 (= T42), E162 (= E160), H194 (= H193)
- binding magnesium ion: S41 (≠ T41), E162 (= E160)
8wm7C Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
42% identity, 84% coverage: 1:211/252 of query aligns to 2:213/658 of 8wm7C
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 90% coverage: 2:228/252 of query aligns to 4:227/369 of P19566