SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_041575701.1 NCBI__GCF_000017645.1:WP_041575701.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
62% identity, 85% coverage: 23:268/289 of query aligns to 4:249/250 of 4hzdA

query
sites
4hzdA

D

D

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D

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I

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A

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x
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C

binding coenzyme a: H158 (= H177), G203 (= G222), A204 (= A223), A221 (= A240), A222 (= A241), T235 (= T254), A237 (= A256), I244 (≠ V263)
binding magnesium ion: S224 (= S243), A237 (= A256), G238 (= G257)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
55% identity, 88% coverage: 28:280/289 of query aligns to 5:257/258 of 4h7oA

query
sites
4h7oA

V

V

W

W

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x
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N

binding arginine: T51 (≠ H74), S53 (≠ D76), Y127 (= Y150), Q131 (≠ R154)
binding cysteine: D88 (= D111), P89 (= P112), D153 (= D176), H154 (= H177), G180 (= G203), R188 (= R211), H189 (= H212)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
55% identity, 88% coverage: 28:280/289 of query aligns to 5:257/258 of 8i04A

query
sites
8i04A

V

V

W

W

A

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H

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N

binding serine: D88 (= D111), P89 (= P112), D153 (= D176), H154 (= H177), G180 (= G203), R188 (= R211), H189 (= H212)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
52% identity, 87% coverage: 28:279/289 of query aligns to 5:256/257 of 1ssqD

query
sites
1ssqD

V

V

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A

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G

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A

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G

A

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K

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L

G

G

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N

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E

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G

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Y

A

A

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K

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I

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A

A

G

N

S

S

V

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K

N

P

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E

N

Y

T

A

T

T

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A

A

A

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G

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P

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M

D

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F

binding cysteine: D88 (= D111), D153 (= D176), H154 (= H177)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
54% identity, 88% coverage: 28:280/289 of query aligns to 9:261/262 of 1t3dA

query
sites
1t3dA

V

V

W

W

A

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R

N

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N

A

E

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A

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K

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T

T

V

A

A

A

G

G

I

V

P

P

A

A

K

R

V

I

V

V

G

G

E

K

A

P

G

D

C

S

A

D

E

K

P

P

S

S

R

M

S

D

M

M

D

D

Q

Q

M

H

F

F

E

N

binding cysteine: D92 (= D111), P93 (= P112), D157 (= D176), G184 (= G203), R192 (= R211), H193 (= H212)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
52% identity, 90% coverage: 25:283/289 of query aligns to 9:267/272 of 3gvdI

query
sites
3gvdI

V

L

D

E

P

Q

V

V

W

W

A

S

R

N

L

I

R

K

N

S

E

E

A

A

E

R

E

A

A

L

A

A

E

E

R

C

E

E

P

P

L

M

L

L

A

A

A

S

F

F

L

F

I

H

G

A

A

T

V

L

L

L

G

K

Q

H

P

E

S

N

L

L

E

G

A

S

V

A

I

L

G

S

E

Y

R

I

I

L

A

A

A

N

R

K

L

L

D

A

H

N

A

P

D

I

V

M

P

P

A

A

S

I

A

A

I

I

R

R

A

E

A

V

W

V

L

E

E

E

A

A

A

Y

A

R

R

S

D

D

K

A

T

H

I

M

P

I

Q

V

A

S

L

A

R

A

A

R

D

D

I

I

M

L

A

A

V

V

V

R

D

L

R

R

D
|
D

P
|
P

A

A

A

V

T

D

R

K

T

Y

L

S

E

T

P

P

V

L

L

L

Y

Y

F

L

K

K

G

G

F

F

H

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

S

G

H

H

F

W

L

L

W

W

A

A

S

Q

G

D

Q

R

R

K

D

A

F

L

A

A

L

I

Y

Y

L

L

Q

Q

S

N

R

Q

A

V

S

S

S

V

V

A

F

F

A

G

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

T

M

I

G

G

R

C

G

G

I

I

F

M

L

L

D
|
D

H

H

A

A

T

T

G

G

V

I

V

V

I

I

G

G

A

E

T

T

A

A

V

V

I

V

E

E

D

N

D

D

V

V

S

S

I

I

L

L

Q

Q

G

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

G

G

K

K

E

T

T

S

G

G

D

D

R
|
R

H

H

P

P

K

K

I

I

R

R

R

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

E

E

V

V

G

G

R

R

C

G

A

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

L

K

Q

P

S

V

V

P

P

R

A

N

H

T

T

T

T

V

A

A

A

G

G

I

V

P

P

A

A

K

R

V

I

V

V

G

G

E

K

A

P

G

E

C

S

A

D

E

K

P

P

S

S

R

L

S

D

M

M

D

D

Q

Q

M

H

F

F

E

N

D

G

N

S

I

I

binding cysteine: D95 (= D111), P96 (= P112), D160 (= D176), G186 (= G202), G187 (= G203), R195 (= R211)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
55% identity, 82% coverage: 28:265/289 of query aligns to 8:245/246 of 8i09A

query
sites
8i09A

V

V

W

W

A

K

R

N

L

I

R

K

N

A

E

E

A

A

E

R

E

A

A

L

A

A

E

D

R

C

E

E

P

P

L

M

L

L

A

A

A

S

F

F

L

Y

I

H

G

A

A

T

V

L

L

L

G

K

Q

H

P

E

S

N

L

L

E

G

A

S

V

A

I

L

G

S

E

Y

R

M

I

L

A

A

A

N

R

K

L

L

D

A

H

S

A

P

D

I

V

M

P

P

A

A

S

I

A

A

I

I

R

R

A

E

A

V

W

V

L

E

E

E

A

A

A

Y

A

A

R

A

D

D

K

P

T

E

I

M

P

I

Q

A

A

S

L

A

R

A

A

C

D

D

I

I

M

Q

A

A

V

V

V

R

D

T

R

R

D
|
D

P

P

A

A

A

V

T

D

R

K

T

Y

L

S

E

T

P

P

V

L

L

L

Y

Y

F

L

K

K

G

G

F

F

H

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

S

G

H

H

F

W

L

L

W

W

A

N

S

K

G

G

Q

R

R

R

D

A

F

L

A

A

L

I

Y

F

L

L

Q

Q

S

N

R

Q

A

V

S

S

S

V

V

S

F

F

A

Q

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

K

M

I

G

G

R

R

G

G

I

I

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

V

I

V

V

I

V

G
|
G

A

E

T

T

A

A

V

V

I

I

E

E

D

D

D

D

V

V

S

S

I

I

L

L

Q

Q

G

S

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

G

K

K

E

T

T

S

G

G

D

D

R
|
R

H
|
H

P

P

K

K

I

I

R

R

R

E

G

G

V

V

L

M

I

I

G
|
G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

E

E

V

V

G

G

R

R

C

G

A

A

R
x
K

I
|
I

A
x
G

A

A

G

G

S

S

V

V

V

V

L
|
L

K

Q

P

P

V

V

P

P

R

P

N

H

T

T

T

T

V

A

A
|
A

G

G

I

V

P

P

A

A

K

R

V

I

V

V

G

G

E

K

binding cysteine: D91 (= D111), D156 (= D176), H157 (= H177), G164 (= G184), R191 (= R211), H192 (= H212)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G203), T184 (= T204), G202 (= G222), K218 (≠ R238), I219 (= I239), G220 (≠ A240), L226 (= L246), A236 (= A256)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
55% identity, 82% coverage: 28:263/289 of query aligns to 9:244/244 of 8i06A

query
sites
8i06A

V

V

W

W

A

K

R

N

L

I

R

K

N

A

E

E

A

A

E

R

E

A

A

L

A

A

E

D

R

C

E

E

P

P

L

M

L

L

A

A

A

S

F

F

L

Y

I

H

G

A

A

T

V

L

L

L

G

K

Q

H

P

E

S

N

L

L

E

G

A

S

V

A

I

L

G

S

E

Y

R

M

I

L

A

A

A

N

R

K

L

L

D

A

H

S

A

P

D

I

V

M

P

P

A

A

S

I

A

A

I

I

R

R

A

E

A

V

W

V

L

E

E

E

A

A

A

Y

A

A

R

A

D

D

K

P

T

E

I

M

P

I

Q

A

A

S

L

A

R

A

A

C

D

D

I

I

M

Q

A

A

V

V

V

R

D

T

R

R

D

D

P
|
P

A

A

A

V

T

D

R

K

T

Y

L

S

E

T

P

P

V

L

L

L

Y

Y

F

L

K

K

G

G

F

F

H

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

S

G

H

H

F

W

L

L

W

W

A

N

S

K

G

G

Q

R

R

R

D

A

F

L

A

A

L

I

Y

F

L

L

Q

Q

S

N

R

Q

A

V

S

S

S

V

V

S

F

F

A

Q

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

K

M

I

G

G

R

R

G

G

I

I

F

M

L

L

D

D

H
|
H

A

A

T

T

G

G

V

I

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

E

E

D

D

D

D

V

V

S

S

I

I

L
|
L

Q
|
Q

G

S

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

T

T

S

G

G

D

D

R

R

H

H

P

P

K

K

I

I

R

R

R

E

G

G

V

V

L

M

I

I

G
|
G

A
|
A

G

G

A

A

K

K

V

I

L

L

G

G

N

N

I

I

E

E

V

V

G

G

R

R

C

G

A

A

R

K

I

I

A
x
G

A
|
A

G

G

S

S

V

V

V

V

L

L

K

Q

P

P

V

V

P

P

R

P

N

H

T

T

T
|
T

V

A

A
|
A

G

G

I

V

P

P

A

A

K

R

V

I

V

V

binding coenzyme a: H158 (= H177), L177 (= L196), Q178 (= Q197), G203 (= G222), A204 (= A223), G221 (≠ A240), A222 (= A241), T235 (= T254), A237 (= A256)
binding cysteine: P93 (= P112), H158 (= H177)

1sstA Serine acetyltransferase- complex with coa (see paper)
50% identity, 82% coverage: 28:263/289 of query aligns to 5:233/233 of 1sstA

query
sites
1sstA

V

V

W

W

A

Q

R

H

L

I

R

R

N

Q

E

E

A

A

E

K

E

E

A

L

A

A

E

E

R

N

E

E

P

P

L

M

L

L

A

A

A

S

F

F

L

F

I

H

G

S

A

T

V

I

L

L

G

K

Q

H

P

Q

S

N

L

L

E

G

A

G

V

A

I

L

G

S

E

Y

R

L

I

L

A

A

A

N

R

K

L

L

D

A

H

N

A

P

D

I

V

M

P

P

A

A

S

I

A

S

I

L

R

R

A

E

A

I

W

I

L

E

E

E

A

A

A

Y

A

Q

R

S

D

N

K

P

T

S

I

I

P

I

Q

D

A

C

L

A

R

A

A

C

D

D

I

I

M

Q

A

A

V

V

V

R

D

H

R

R

D

D

P

P

A

A

A

V

T

E

R

L

T

W

L

S

E

T

P

P

V

L

L

L

Y

Y

F

L

K

K

G

G

F

F

H

H

A

A

I

I

Q

Q

T

S

H

Y

R

R

L

I

S

T

H

H

F

Y

L

L

W

W

A

N

S

Q

G

N

Q

R

R

K

D

S

F

L

A

A

L

L

Y

Y

L

L

Q

Q

S

N

R

Q

A

I

S

S

S

V

V

A

F

F

A

D

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

K

M

I

G

G

R

H

G

G

I

I

F

M

L

F

D

D

H
|
H

A

A

T

T

G

G

V

I

V

V

I

V

G

G

A

E

T

T

A

S

V

V

I

I

E

E

D

N

D

D

V

V

S

S

I

I

L
|
L

Q

Q

G

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

-

K

-

E

-

T

-

G

-

D

-

R

-

H

H

P

P

K

K

I

V

R

R

R

E

G

G

V

V

L

M

I

I

G

G

A
|
A

G

G

A

A

K

K

V

I

L
|
L

G

G

N

N

I

I

E

E

V

V

G

G

R

K

C

Y

A

A

R
x
K

I

I

A
x
G

A
|
A

G

N

S

S

V
|
V

V

V

L
|
L

K

N

P

P

V

V

P

P

R

E

N

Y

T

A

T
|
T

V

A

A
|
A

G

G

I
x
V

P
|
P

A

A

K

R

V

I

V
|
V

binding coenzyme a: H154 (= H177), L173 (= L196), G180 (= G203), T181 (= T204), A193 (= A223), L198 (= L228), K208 (≠ R238), G210 (≠ A240), A211 (= A241), V214 (= V244), L216 (= L246), T224 (= T254), A226 (= A256), V228 (≠ I258), P229 (= P259), V233 (= V263)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
47% identity, 88% coverage: 28:280/289 of query aligns to 10:261/261 of 6wyeA

query
sites
6wyeA

V

L

W

W

A

H

R

T

L

I

R

R

N

E

E

E

A

T

E

A

E

A

A

A

A

A

E

A

R

A

E

E

P

P

L

M

L

L

A

A

A

S

F

F

L

L

I

H

G

Q

A

T

V

V

L

L

G

R

Q

H

P

E

S

S

L

L

E

G

A

S

V

V

I

L

G

A

E

Y

R

H

I

L

A

S

A

S

R

K

L

L

D

G

H

S

A

P

D

I

V

M

P

D

A

V

S

R

A

A

I

L

R

F

A

E

A

I

W

Y

L

Q

E

Q

A

A

A

L

A

G

R

S

D

D

K

T

T

Q

I

I

P

S

Q

K

A

C

L

V

R

E

A

A

D

D

I

L

M

K

A

A

V

I

V

Y

D

E

R

R

D
|
D

P
|
P

A

A

A

C

T

D

R

E

T

Y

L

S

E

L

P

P

V

L

L

L

Y

Y

F

F

K

K

G

G

F

F

H

H

A

A

I

I

Q

Q

T

A

H

H

R

R

L

I

S

N

H

H

F

R

L

L

W

Y

A

L

S

D

G

G

Q

R

R

K

D

T

F

L

A

A

L

Y

Y

F

L

L

Q

Q

S

N

R

R

A

M

S

S

S

E

V

V

F

F

A

G

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

R

M

L

G

G

R

Y

G

G

I

L

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

V

F

V

V

I

A

G

G

A

E

T

T

A

A

V

V

I

L

E

G

D

N

D

N

V

I

S

S

I

I

L

L

Q

H

G

G

V

V

T

T

L

L

G

G

G
|
G

T

S

G

G

K

K

E

E

T

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

I

I

R

G

R

D

G

G

V

V

L

M

I

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

N

N

I

I

E

R

V

I

G

G

R

S

C

N

A

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

V

K

S

P

D

V

V

P

P

R

P

N

S

T

I

T

T

V

V

A

V

G

G

I

V

P

P

A

A

K

K

V

P

V

V

G

A

E

R

A

S

G

-

C

L

A

K

E

T

P

P

S

S

R

A

S

D

M

M

D

D

Q

Q

M

N

F

I

E

Q

binding (2S)-2-hydroxybutanedioic acid: D93 (= D111), P94 (= P112), D158 (= D176), H159 (= H177), G185 (= G203), R193 (= R211), H194 (= H212)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
49% identity, 82% coverage: 28:263/289 of query aligns to 8:243/243 of 7ra4A

query
sites
7ra4A

V

L

W

W

A

H

R

T

L

I

R

R

N

E

E

E

A

T

E

A

E

A

A

A

A

A

E

A

R

A

E

E

P

P

L

M

L

L

A

A

A

S

F

F

L

L

I

H

G

Q

A

T

V

V

L

L

G

R

Q

H

P

E

S

S

L

L

E

G

A

S

V

V

I

L

G

A

E

Y

R

H

I

L

A

S

A

S

R

K

L

L

D

G

H

S

A

P

D

I

V

M

P

D

A

V

S

R

A

A

I

L

R

F

A

E

A

I

W

Y

L

Q

E

Q

A

A

A

L

A

G

R

S

D

D

K

T

T

Q

I

I

P

S

Q

K

A

C

L

V

R

E

A

A

D

D

I

L

M

K

A

A

V

I

V

Y

D

E

R

R

D
|
D

P
|
P

A

A

A

C

T

D

R

E

T

Y

L

S

E

L

P

P

V

L

L

L

Y

Y

F

F

K

K

G

G

F

F

H

H

A

A

I

I

Q

Q

T

A

H

H

R

R

L

I

S

N

H

H

F

R

L

L

W

Y

A

L

S

D

G

G

Q

R

R

K

D

T

F

L

A

A

L

Y

Y

F

L

L

Q

Q

S

N

R

R

A

M

S

S

S

E

V

V

F

F

A

G

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

R

M

L

G

G

R

Y

G

G

I

L

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

V

F

V

V

I

A

G

G

A

E

T

T

A

A

V

V

I

L

E

G

D

N

D

N

V

I

S

S

I

I

L

L

Q

H

G

G

V

V

T

T

L

L

G

G

G

G

T

S

G

G

K

K

E

E

T

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

I

I

R

G

R

D

G

G

V

V

L

M

I

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

N

N

I

I

E

R

V

I

G

G

R

S

C

N

A

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

V

K

S

P

D

V

V

P

P

R

P

N

S

T

I

T

T

V

V

A

V

G

G

I

V

P

P

A

A

K

K

V

P

V

V

binding serine: D91 (= D111), P92 (= P112), D156 (= D176), H157 (= H177), R191 (= R211), H192 (= H212)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
48% identity, 82% coverage: 28:263/289 of query aligns to 8:243/243 of 4n69A

query
sites
4n69A

V

V

W

W

A

G

R

Q

L

I

R

K

N

A

E

E

A

A

E

R

E

R

A

D

A

A

E

E

R

S

E

E

P

P

L

A

L

L

A

A

A

S

F

Y

L

L

I

Y

G

S

A

T

V

I

L

L

G

S

Q

H

P

S

S

S

L

L

E

E

A

R

V

S

I

L

G

S

E

F

R

H

I

L

A

G

A

N

R

K

L

L

D

C

H

S

A

S

D

T

V

L

P

L

A

S

S

T

A

L

I

L

R

Y

A

D

A

L

W

F

L

L

E

N

A

A

A

F

A

S

R

S

D

D

K

P

T

S

I

L

P

R

Q

S

A

A

L

A

R

V

A

A

D

D

I

L

M

R

A

A

V

A

V

R

D

E

R

R

D

D

P

P

A

A

A

C

T

V

R

S

T

Y

L

S

E

H

P

C

V

L

L

L

Y

N

F

Y

K

K

G

G

F

F

H

L

A

A

I

C

Q

Q

T

A

H

H

R

R

L

V

S

A

H

H

F

L

L

L

W

W

A

R

S

Q

G

S

Q

R

R

R

D

P

F

L

A

A

L

L

Y

A

L

L

Q

H

S

S

R

R

A

I

S

A

S

N

V

V

F

F

A

A

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

R

M

I

G

G

R

K

G

G

I

I

F

L

L

F

D

D

H

H

A

A

T

T

G

G

V

V

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

E

G

D

N

D

N

V

V

S

S

I

I

L

L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

V

T

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

I

I

R

G

R

D

G

G

V

V

L

L

I

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

N

N

I

I

E

K

V

I

G

G

R

E

C

G

A

A

R

K

I

V

A

G

A

A

G

G

S

S

V

V

V

V

L

L

K

I

P

D

V

V

P

P

R

P

N

R

T

T

T

T

V

A

A

V

G

G

I

N

P

P

A

A

K

R

V

L

V

V

binding serine: G183 (= G203), R191 (= R211), H192 (= H212)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
47% identity, 82% coverage: 28:263/289 of query aligns to 4:233/233 of 4n6bA

query
sites
4n6bA

V

V

W

W

A

G

R

Q

L

I

R

K

N

A

E

E

A

A

E

R

E

R

A

D

A

A

E

E

R

S

E

E

P

P

L

A

L

L

A

A

A

S

F

Y

L

L

I

Y

G

S

A

T

V

I

L

L

G

S

Q

H

P

S

S

S

L

L

E

E

A

R

V

S

I

L

G

S

E

F

R

H

I

L

A

G

A

N

R

K

L

L

D

C

H

S

A

S

D

T

V

L

P

L

A

S

S

T

A

L

I

L

R

Y

A

D

A

L

W

F

L

L

E

N

A

A

A

F

A

S

R

S

D

D

K

P

T

S

I

L

P

R

Q

S

A

A

L

A

R

V

A

A

D

D

I

L

M

R

A

A

V

A

V

R

D

E

R

R

D

D

P

V

A

S

A

Y

T

S

R

H

T

C

L

L

E

-

P

-

V

-

L

L

Y

N

F

Y

K

K

G

G

F

F

H

L

A

A

I

C

Q

Q

T

A

H

H

R

R

L

V

S

A

H

H

F

L

L

L

W

W

A

R

S

Q

G

S

Q

R

R

R

D

P

F

L

A

A

L

L

Y

A

L

L

Q

H

S

S

R

R

A

I

S

A

S

N

V

V

F

F

A

A

V

V

D

D

I

I

H

H

P

P

Q

A

V

A

P

R

M

I

G

G

R

K

G

G

I

I

F

L

L

F

D

D

H
|
H

A

A

T

T

G

G

V

V

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

E

G

D

N

D

N

V

V

S

S

I

I

L
|
L

Q

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

-

E

-

T

-

G

G

D

D

R

R

H

H

P

P

K

K

I

I

R

G

R

D

G

G

V

V

L

L

I

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

N

N

I

I

E

K

V

I

G

G

R

E

C

G

A

A

R
x
K

I

V

A
x
G

A
|
A

G

G

S

S

V

V

V

V

L
|
L

K

I

P

D

V

V

P

P

R

P

N

R

T

T

T

T

V

A

A
x
V

G

G

I

N

P

P

A

A

K

R

V

L

V

V

binding coenzyme a: H150 (= H177), L169 (= L196), G176 (= G203), K208 (≠ R238), G210 (≠ A240), A211 (= A241), L216 (= L246), V226 (≠ A256)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
33% identity, 69% coverage: 57:255/289 of query aligns to 73:258/280 of 7bw9A

query
sites
7bw9A

Q

Q

P

N

S

S

L

I

E

E

A

A

V

Y

I

Y

G

N

E

Q

R

Q

I

L

A

A

A

E

R

K

L

C

D

-

H

-

A

-

D

-

V

-

P

-

A

I

S

T

A

A

I

L

R

L

A

S

A

Q

W

F

L

V

E

-

A

-

A

-

A

-

R

-

D

-

K

-

T

T

I

I

P

R

Q

E

A

L

L

V

R

K

A

Q

D

D

I

I

M

I

A

A

V

A

V

Y

D

T

R

G

D
|
D

P
|
P

A

A

A

A

T

S

R

S

T

L

L

A

E

M

P

I

V

I

L

R

Y

S

F

Y

K

P

G

G

F

I

H

H

A

V

I

M

Q

M

T

I

H

Q

R

R

L

V

S

A

H

H

F

I

L

L

W

Y

A

M

S

N

G

G

Q

D

R

I

D

E

F

Y

A

S

L

R

Y

E

L

L

Q

M

S

E

R

N

A

I

S

H

S

S

V

V

F

T

A

G

V

I

D

D

I

I

H

H

P

P

Q

G

V

T

P

S

M

I

G

G

R

N

G

H

I

F

F

F

L

I

D
|
D

H
|
H

A

G

T

V

G

G

V

V

V

V

I

I

G

G

A

E

T

T

A

A

V

V

I

I

E

G

D

N

D

W

V

C

S

R

I

V

L

Y

Q

Q

G

S

V

V

T

T

L

L

G

G

G
x
A

T

M

G

S

K

F

E

K

T

G

G

N

D

K

R
|
R

H
|
H

P

P

K

T

I

I

R

G

R

D

G

F

V

V

L

V

I

I

G

G

A

A

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

E

T

V

I

G

G

R

S

C

N

A

V

R

K

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

T

K

Q

P

N

V

I

P

D

R

Q

N

D

T

Q

T

I

V

V

binding cysteine: D114 (= D111), P115 (= P112), D179 (= D176), H180 (= H177), A206 (≠ G203), R214 (= R211), H215 (= H212)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
37% identity, 56% coverage: 95:255/289 of query aligns to 100:268/270 of 3p47A

query
sites
3p47A

T

S

I

I

P

K

Q

R

A

T

L

L

R

K

A

T

D

D

I

L

M

I

A

A

V

A

V

Y

D

A

R

G

D

D

P

P

A

A

A

A

T

P

R

G

T

L

L

S

E

L

P

I

V

I

L

R

Y

C

F

Y

K

P

G

G

F

F

H

Q

A

A

I

V

Q

I

T

V

H

Y

R

R

L

I

S

A

H

H

F

V

L

L

W

Y

A

E

S

C

G

G

Q

E

R

R

D

Y

F

Y

A

C

L

R

Y

E

L

M

Q

M

S

E

R

S

A

V

S

H

S

S

V

Y

F

T

A

S

V

I

D

D

I

I

H

H

P

P

Q

G

V

A

P

S

M

I

G

K

R

G

G

H

I

F

F

F

L

I

D

D

H

H

A

G

T

V

G

G

V

V

V

V

I

I

G

G

A

E

T

T

A

A

V

I

I

I

E

G

D

E

D

W

V

C

S

R

I

I

L

Y

Q

Q

G

S

V

V

T

T

L

L

-

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

T

G

G

T

D

K

R
|
R

H
|
H

P

P

K

T

I

V

R

G

R

D

G

Y

V

V

L

T

I

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

E

I

V

V

G

G

R

S

C

H

A

V

R

R

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

R

S

N

N

T

Q

T

T

V

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R211), H225 (= H212)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
37% identity, 56% coverage: 95:255/289 of query aligns to 98:266/267 of 3q1xA

query
sites
3q1xA

T

S

I

I

P

K

Q

R

A

T

L

L

R

K

A

T

D

D

I

L

M

I

A

A

V

A

V

Y

D

A

R

G

D

D

P

P

A

A

A

A

T

P

R

G

T

L

L

S

E

L

P

I

V

I

L

R

Y

C

F

Y

K

P

G

G

F

F

H

Q

A

A

I

V

Q

I

T

V

H

Y

R

R

L

I

S

A

H

H

F

V

L

L

W

Y

A

E

S

C

G

G

Q

E

R

R

D

Y

F

Y

A

C

L

R

Y

E

L

M

Q

M

S

E

R

S

A

V

S

H

S

S

V

Y

F

T

A

S

V

I

D

D

I

I

H

H

P

P

Q

G

V

A

P

S

M

I

G

K

R

G

G

H

I

F

F

F

L

I

D

D

H

H

A

G

T

V

G

G

V

V

V

V

I

I

G

G

A

E

T

T

A

A

V

I

I

I

E

G

D

E

D

W

V

C

S

R

I

I

L

Y

Q

Q

G

S

V

V

T

T

L

L

-

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

T

G

G

T

D

K

R
|
R

H
|
H

P

P

K

T

I

V

R

G

R

D

G

Y

V

V

L

T

I

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

N

N

I

I

E

I

V

V

G

G

R

S

C

H

A

V

R

R

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

R

S

N

N

T

Q

T

T

V

V

binding serine: H208 (vs. gap), R222 (= R211), H223 (= H212)

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
31% identity, 34% coverage: 181:278/289 of query aligns to 47:156/294 of 4mzuF

query
sites
4mzuF

V

V

V

V

I

I

G

G

A

D

T

N

A

V

V

T

I

I

E

K

D

S

D

G

V

V

S

Q

I

I

L

W

Q

D

G

G

-

I

-

H

-

I

-

Q

-

D

-

D

V

V

T

F

L

I

G

G

G

P

T

N

G

V

K

T

E

F

T

T

G
x
N

D
|
D

R
x
K

H

Q

P

P

K

R

-

S

-

K

-

I

-

K

-

T

-

I

I

V

R

K

R

K

G

G

V

A

L

S

I

I

G
|
G

A
|
A

G

N

A

S

K

T

V

I

L
|
L

G

P

N

G

I

I

E

L

V

I

G

G

R

E

C

N

A

A

R

M

I

V

A
x
G

A
|
A

G

G

S

A

V

V

V

I

L
x
T

K
|
K

P

N

V

V

P

P

R

D

N

N

T

A

T

I

V

V

A
x
I

G

G

I
x
N

P

P

A

G

K
x
R

V

I

V

T

G

G

E

Y

A

V

G

E

C

A

A

F

E

E

P

L

S

K

R

L

S

Q

M

M

D

S

Q

K

M

V

binding coenzyme a: N81 (≠ G209), D82 (= D210), K83 (≠ R211), G100 (= G222), A101 (= A223), L106 (= L228), G118 (≠ A240), A119 (= A241), T124 (≠ L246), K125 (= K247), I134 (≠ A256), N136 (≠ I258), R139 (≠ K261)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
34% identity, 29% coverage: 181:264/289 of query aligns to 47:141/290 of 4mzuB

query
sites
4mzuB

V

V

V

V

I

I

G

G

A

D

T

N

A

V

V

T

I

I

E

K

D

S

D
x
G

V

V

S

Q

I

I

L

W

Q

D

G

G

-

I

-

H

-

I

-

Q

-

D

-

D

V

V

T
x
F

L

I

G

G

G

P

T
x
N

G

V

K

T

E

F

T
|
T

G
x
N

D
|
D

R
x
K

H

Q

P
|
P

K

R

-

S

-

L

-

K

-

T

-

I

I

V

R

K

R

K

G

G

V

A

L

S

I

I

G
|
G

A
|
A

G

N

A

S

K

T

V

I

L

L

G

P

N

G

I

I

E

L

V

I

G

G

R

E

C

N

A

A

R

M

I

V

A
x
G

A
|
A

G

G

S

A

V

V

V

I

L
x
T

K
|
K

P

N

V

V

P

P

R

D

N

N

T

A

T

I

V

V

A
x
I

G

G

I

N

P

P

A

G

K
x
R

V

I

V

T

G

G

binding coenzyme a: F72 (≠ T200), T80 (= T208), N81 (≠ G209), D82 (= D210), K83 (≠ R211), P85 (= P213), G99 (= G222), A100 (= A223), G117 (≠ A240), A118 (= A241), T123 (≠ L246), K124 (= K247), I133 (≠ A256), R138 (≠ K261)
binding magnesium ion: G58 (≠ D192), N76 (≠ T204)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
28% identity, 40% coverage: 166:281/289 of query aligns to 74:195/201 of 1krvA

query
sites
1krvA

Q

N

V

I

P

H

M

I

G

G

R

R

G

N

I

F

F
x
Y

L

A

D
x
N

H

F

A

N

T

L

G

T

V

I

V
|
V

I

D

G
x
D

A

Y

T

T

A

V

V

T

I

I

E

G

D

D

D

N

V

V

S

L

I

I

L
x
A

Q

P

G

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

G

G

-

H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

T

N

G

G

D

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

K

T

I

I

R

G

R

N

G

N

V

V

L
x
W

I

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

N

G

I

V

E

T

V

I

G

G

R

D

C

N

A

S

R

V

I

I

A

G

A
|
A

G

G

S

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

R

P

N

N

T

V

T

V

V

A

A

A

G

G

I

V

P
|
P

A

C

K
x
R

V

V

V

I

G
x
R

E

E

A

I

G

N

C

-

A

-

E

-

P

-

S

D

R

R

S

D

M

K

D

H

Q

Y

M

Y

F

F

E

K

D

D

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F174), N84 (≠ D176), V90 (= V182), D92 (≠ G184), M126 (≠ R211)
binding coenzyme a: A104 (≠ L196), S110 (≠ G202), T112 (= T204), W138 (≠ L220), G140 (= G222), S141 (≠ A223), N146 (≠ L228), A159 (= A241), P177 (= P259), R179 (≠ K261), R182 (≠ G264)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
28% identity, 40% coverage: 166:281/289 of query aligns to 74:195/201 of 1kruA

query
sites
1kruA

Q

N

V

I

P

H

M

I

G

G

R

R

G

N

I

F

F
x
Y

L

A

D
x
N

H

F

A

N

T

L

G

T

V

I

V
|
V

I

D

G
x
D

A

Y

T

T

A

V

V

T

I

I

E

G

D

D

D

N

V

V

S
x
L

I

I

L

A

Q

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

T

N

G

G

D

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

K

T

I

I

R

G

R

N

G

N

V

V

L
x
W

I

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

N

G

I

V

E

T

V

I

G

G

R

D

C

N

A

S

R

V

I

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

R

P

N

N

T

V

T

V

V

A

A

A

G

G

I

V

P
|
P

A

C

K

R

V

V

V

I

G
x
R

E

E

A

I

G

N

C

-

A

-

E

-

P

-

S

D

R

R

S

D

M

K

D

H

Q

Y

M

Y

F

F

E

K

D

D

binding coenzyme a: H114 (vs. gap), W138 (≠ L220), G140 (= G222), S141 (≠ A223), G158 (≠ A240), A159 (= A241), P177 (= P259), R182 (≠ G264)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ F174), Y82 (≠ F174), N84 (≠ D176), V90 (= V182), D92 (≠ G184), L102 (≠ S194), H114 (vs. gap), M126 (≠ R211)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

Query Sequence

>WP_041575701.1 NCBI__GCF_000017645.1:WP_041575701.1
MPAPVADAFAPKLTKAGTERWHDVVDPVWARLRNEAEEAAEREPLLAAFLIGAVLGQPSL
EAVIGERIAARLDHADVPASAIRAAWLEAAARDKTIPQALRADIMAVVDRDPAATRTLEP
VLYFKGFHAIQTHRLSHFLWASGQRDFALYLQSRASSVFAVDIHPQVPMGRGIFLDHATG
VVIGATAVIEDDVSILQGVTLGGTGKETGDRHPKIRRGVLIGAGAKVLGNIEVGRCARIA
AGSVVLKPVPRNTTVAGIPAKVVGEAGCAEPSRSMDQMFEDNIWSGADI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory