SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
32% identity, 43% coverage: 9:230/518 of query aligns to 3:222/358 of 8y5iA

query
sites
8y5iA

L

V

Q

Q

L

L

C

A

G

G

I

I

T

R

K

K

Q

C

Y
x
F

P

D

G

G

C
x
K

L

E

A

V

N

I

D

P

R

Q

I

L

D

D

L

L

S

T

I

I

Q

N

P

N

G

G

E

E

I

F

H

L

A

T

L

L

G

G

E

P

N

S

G
|
G

A

C

G

G

K
|
K

S
x
T

T
|
T

L

V

M

L

K

R

I

L

I

I

Y

A

G

G

V

L

T

E

Q

T

P

V

D

D

A

S

G

G

A

R

I

I

H

M

W

L

Q

D

G

N

E

E

R

D

V

I

T

T

M

H

R

-

D

-

P

-

A

V

Q

P

A

A

R

E

E

N

R

R

G

Y

I

V

G

N

M

T

V

V

F

F

Q

Q

H

S

F

Y

S

A

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

F

E

E

N

N

I

V

A

A

L

F

A

G

L

L

G

R

A

M

K

Q

A

K

G

T

T

P

P

A

K

A

Q

E

L

I

E

T

P

P

K

R

I

V

R

M

E

E

V

A

S

L

Q

R

R

M

Y

V

G

Q

M

L

P

E

L

T

E

F

P

A

Q

Q

R
|
R

L

K

V

P

H

H

S
x
Q

L

L

S
|
S

I

G

G

G

E

Q

R

Q

Q

Q

R

R

V

V

E

A

I

I

V

A

R

R

C

A

L

V

M

V

Q

N

D

K

I

P

R

R

L

L

I

L

L

L

D

D

E

Q

P

S

T

L

S

S

V

A

L

L

T

D

-

Y

-

K

-

L

-

R

P

K

Q

Q

E

M

A

Q

D

N

E

E

L

L

F

K

V

A

T

L

L

Q

R

R

R

K

L

L

A

-

E

-

G

G

C

I

S

T

I

F

L

V

F

F

I

V

S

T

H

H

K

D

L

Q

N

E

E

E

V

A

R

L

A

T

L

M

C

S

Q

D

S

R

A

I

T

V

V

V

L

M

R

R

G

D

G

G

R

R

V

I

S

E

G

Q

E

D

C

G

I

T

P

P

A

R

E

E

C

I

S

Y

D

E

binding adenosine-5'-triphosphate: F12 (≠ Y18), K15 (≠ C21), G38 (= G44), K41 (= K47), T42 (≠ S48), T43 (= T49), R128 (= R137), Q132 (≠ S141), S134 (= S143)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 45% coverage: 7:239/518 of query aligns to 2:232/369 of P19566

query
sites
P19566

A

A

R

S

L

V

Q

Q

L

L

C

R

G

N

I

V

T

T

K

K

Q

A

Y

W

P

G

G

D

C

V

L

V

A

V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

D

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

T

R

R

V

M

T

N

M

D

R

I

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S

A

L
|
L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

M

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

P

A

L

H

E

L

P

L

Q

E

R

R

L

K

V

P

H

K

S

A

L

L

S

S

I

G

G

G

E

Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

R

L

V

L

F

I

L

L

L

D

D

E

E

P
|
P

T

L

S

S

V

N

L

L

T
x
D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R

R

L

F

M

V

V

A

G

G

L86 (= L94) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P168) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (≠ T173) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
31% identity, 45% coverage: 7:239/518 of query aligns to 1:231/374 of 2awnB

query
sites
2awnB

A

A

R

S

L

V

Q

Q

L

L

C

Q

G

N

I

V

T

T

K

K

Q

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

K

R

R

V

M

T

N

M

D

R

T

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

P

A

L

H

E

L

P

L

Q

D

R

R

L

K

V

P

H

K

S

A

L

L

S

S

I

G

G

G

E

Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R

R

L

F

M

V

V

A

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y18), V17 (≠ A23), S37 (≠ N43), G38 (= G44), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 45% coverage: 7:239/518 of query aligns to 1:231/371 of 3puyA

query
sites
3puyA

A

A

R

S

L

V

Q

Q

L

L

C

Q

G

N

I

V

T

T

K

K

Q

A

Y
x
W

P

G

G

E

C

V

L

V

A

V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

K

R

R

V

M

T

N

M

D

R

T

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

P

A

L

H

E

L

P

L

Q

D

R
|
R

L

K

V

P

H

K

S
x
A

L

L

S
|
S

I

G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E
|
E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H
|
H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R

R

L

F

M

V

V

A

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y18), S37 (≠ N43), G38 (= G44), C39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), Q81 (= Q90), R128 (= R137), A132 (≠ S141), S134 (= S143), G136 (= G145), Q137 (≠ E146), E158 (= E167), H191 (= H199)
binding magnesium ion: S42 (= S48), Q81 (= Q90)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 45% coverage: 7:239/518 of query aligns to 1:231/371 of 3puxA

query
sites
3puxA

A

A

R

S

L

V

Q

Q

L

L

C

Q

G

N

I

V

T

T

K

K

Q

A

Y
x
W

P

G

G

E

C

V

L

V

A

V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

K

R

R

V

M

T

N

M

D

R

T

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

P

A

L

H

E

L

P

L

Q

D

R
|
R

L

K

V

P

H

K

S

A

L

L

S
|
S

I

G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H
|
H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R

R

L

F

M

V

V

A

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y18), G38 (= G44), C39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), R128 (= R137), S134 (= S143), Q137 (≠ E146)
binding beryllium trifluoride ion: S37 (≠ N43), G38 (= G44), K41 (= K47), Q81 (= Q90), S134 (= S143), G136 (= G145), H191 (= H199)
binding magnesium ion: S42 (= S48), Q81 (= Q90)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
31% identity, 45% coverage: 7:239/518 of query aligns to 1:231/371 of 3puwA

query
sites
3puwA

A

A

R

S

L

V

Q

Q

L

L

C

Q

G

N

I

V

T

T

K

K

Q

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

K

R

R

V

M

T

N

M

D

R

T

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

P

A

L

H

E

L

P

L

Q

D

R
|
R

L

K

V

P

H

K

S
x
A

L

L

S
|
S

I
x
G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E
|
E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H
|
H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R

R

L

F

M

V

V

A

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y18), V17 (≠ A23), G38 (= G44), C39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), R128 (= R137), A132 (≠ S141), S134 (= S143), Q137 (≠ E146)
binding tetrafluoroaluminate ion: S37 (≠ N43), G38 (= G44), K41 (= K47), Q81 (= Q90), S134 (= S143), G135 (≠ I144), G136 (= G145), E158 (= E167), H191 (= H199)
binding magnesium ion: S42 (= S48), Q81 (= Q90)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
31% identity, 45% coverage: 7:239/518 of query aligns to 1:231/371 of 3puvA

query
sites
3puvA

A

A

R

S

L

V

Q

Q

L

L

C

Q

G

N

I

V

T

T

K

K

Q

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

K

R

R

V

M

T

N

M

D

R

T

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

P

A

L

H

E

L

P

L

Q

D

R
|
R

L

K

V

P

H

K

S
x
A

L

L

S
|
S

I

G

G

G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R

R

L

F

M

V

V

A

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y18), V17 (≠ A23), G38 (= G44), C39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), R128 (= R137), A132 (≠ S141), S134 (= S143), Q137 (≠ E146)
binding magnesium ion: S42 (= S48), Q81 (= Q90)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 45% coverage: 7:239/518 of query aligns to 2:232/371 of P68187

query
sites
P68187

A

A

R

S

L

V

Q

Q

L

L

C

Q

G

N

I

V

T

T

K

K

Q

A

Y

W

P

G

G

E

C

V

L

V

A

V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

K

R

R

V

M

T

N

M

D

R

T

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S
x
A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T
x
K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I
x
V

R

N

E

Q

V
|
V

S

A

Q
x
E

R

V

Y

L

G

Q

M

L

P
x
A

L

H

E

L

P

L

Q

D

R

R

L

K

V

P

H

K

S

A

L

L

S

S

I

G

G
|
G

E

Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D
|
D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R
|
R

L

F

M

V

V

A

G

G

A85 (≠ S93) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ T114) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ I122) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V125) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ Q127) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ P132) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G145) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D166) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R235) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
30% identity, 41% coverage: 9:219/518 of query aligns to 4:217/230 of 6z4wA

query
sites
6z4wA

L

I

Q

E

L

M

C

R

G

D

I

V

T

V

K

K

Q

K

Y
|
Y

P

D

-

N

G

G

C
x
T

L

T

A
|
A

N

L

D

R

R

G

I

V

D

S

L

V

S

S

I

V

Q

Q

P

P

G

G

E

E

I

F

H

A

A

Y

L

I

L

V

G

G

E

P

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

I

K

R

I

S

I

L

Y

Y

G

R

V

E

T

V

Q

K

P

I

D

D

A

K

G

G

A

S

I

L

H

S

W

V

Q

A

G

G

-

F

-

N

-

L

E

V

R

K

V

I

T

K

M

K

R

K

D

D

P

V

A

P

Q

L

A

L

R

R

E

-

R

R

G

S

I

V

G

G

M

V

V

V

F

F

Q

Q

H

D

F

Y

S

K

L

L

F

L

E

P

T

K

L

K

S

T

V

V

A

Y

E

E

N

N

I

I

A

A

L

Y

A

A

L

M

G

E

A

V

K

I

A

G

G

E

T

N

P

R

K

R

Q

N

L

I

E

K

P

R

K

R

I

V

R

M

E

E

V

V

S

L

Q

D

R

L

Y

V

G

G

M

L

P

K

L

H

E

K

P

V

Q

R

R

S

L

F

V

P

H

N

S

E

L

L

S

S

I

G

G

G

E

E

R

Q

Q

Q

R

R

V

I

E

A

I

I

V

A

R

R

C

A

L

I

M

V

Q

N

D

N

I

P

R

K

L

V

L

L

I

I

L

A

D

D

E

E

P

P

T

T

S

G

V

N

L

L

T

D

P

P

Q

D

E

N

A

S

D

W

E

E

L

I

F

M

V

N

T

L

L

L

R

E

R

R

L

I

A

N

A

L

E

Q

G

G

C

T

S

T

I

I

L

L

F

M

I

A

S

T

H

H

K

N

L

S

N

Q

E

I

V

V

R

N

A

T

L

L

C

R

Q

H

S

R

A

V

T

I

V

A

L

I

R

E

G

N

G

G

R

R

V

V

binding adenosine-5'-diphosphate: Y13 (= Y18), T17 (≠ C21), A19 (= A23), G40 (= G44), G42 (= G46), K43 (= K47), S44 (= S48), T45 (= T49)

A0A0H2ZM82 Cell division ATP-binding protein FtsE from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
30% identity, 41% coverage: 9:219/518 of query aligns to 4:217/230 of A0A0H2ZM82

query
sites
A0A0H2ZM82

L

I

Q

E

L

M

C

R

G

D

I

V

T

V

K

K

Q

K

Y

Y

P

D

-

N

G

G

C

T

L

T

A

A

N

L

D

R

R

G

I

V

D

S

L

V

S

S

I

V

Q

Q

P

P

G

G

E

E

I

F

H

A

A

Y

L

I

L

V

G

G

E

P

N

S

G

G

A

A

G

G

K
|
K

S

S

T

T

L

F

M

I

K

R

I

S

I

L

Y

Y

G

R

V

E

T

V

Q

K

P

I

D

D

A

K

G

G

A

S

I

L

H

S

W

V

Q

A

G

G

-

F

-

N

-

L

E

V

R

K

V

I

T

K

M

K

R

K

D

D

P

V

A

P

Q

L

A

L

R

R

E

-

R

R

G

S

I

V

G

G

M

V

V

V

F

F

Q

Q

H

D

F

Y

S

K

L

L

F

L

E

P

T

K

L

K

S

T

V

V

A

Y

E

E

N

N

I

I

A

A

L

Y

A

A

L

M

G

E

A

V

K

I

A

G

G

E

T

N

P

R

K

R

Q

N

L

I

E

K

P

R

K

R

I

V

R

M

E

E

V

V

S

L

Q

D

R

L

Y

V

G

G

M

L

P

K

L

H

E

K

P

V

Q

R

R

S

L

F

V

P

H

N

S

E

L

L

S

S

I

G

G

G

E

E

R

Q

Q

Q

R

R

V

I

E

A

I

I

V

A

R

R

C

A

L

I

M

V

Q

N

D

N

I

P

R

K

L

V

L

L

I

I

L

A

D
|
D

E
|
E

P

P

T

T

S

G

V

N

L

L

T

D

P

P

Q

D

E

N

A

S

D

W

E

E

L

I

F

M

V

N

T

L

L

L

R

E

R

R

L

I

A

N

A

L

E

Q

G

G

C

T

S

T

I

I

L

L

F

M

I

A

S

T

H

H

K

N

L

S

N

Q

E

I

V

V

R

N

A

T

L

L

C

R

Q

H

S

R

A

V

T

I

V

A

L

I

R

E

G

N

G

G

R

R

V

V

K43 (= K47) mutation to A: Growth cessation and formation of spherical cells with aberrant division planes.
D164 (= D166) mutation to A: Growth cessation and formation of spherical cells with aberrant division planes.
E165 (= E167) mutation to A: Growth cessation and formation of spherical cells with aberrant division planes.

6z67B Ftse structure of streptococcus pneumoniae in complex with amppnp at 2.4 a resolution (see paper)
30% identity, 41% coverage: 9:219/518 of query aligns to 4:217/229 of 6z67B

query
sites
6z67B

L

I

Q

E

L

M

C

R

G

D

I

V

T

V

K

K

Q

K

Y
|
Y

P

D

-

N

G
|
G

C

T

L

T

A
|
A

N

L

D

R

R

G

I

V

D

S

L

V

S

S

I

V

Q

Q

P

P

G

G

E

E

I

F

H

A

A

Y

L

I

L

V

G

G

E

P

N
x
S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

I

K

R

I

S

I

L

Y

Y

G

R

V

E

T

V

Q

K

P

I

D

D

A

K

G

G

A

S

I

L

H

S

W

V

Q

A

G

G

-

F

-

N

-

L

E

V

R

K

V

I

T

K

M

K

R

K

D

D

P

V

A

P

Q

L

A

L

R

R

E

-

R

R

G

S

I

V

G

G

M

V

V

V

F

F

Q

Q

H

D

F

Y

S

K

L

L

F

L

E

P

T

K

L

K

S

T

V

V

A

Y

E

E

N

N

I

I

A

A

L

Y

A

A

L

M

G

E

A

V

K

I

A

G

G

E

T

N

P

R

K

R

Q

N

L

I

E

K

P

R

K

R

I

V

R

M

E

E

V

V

S

L

Q

D

R

L

Y

V

G

G

M

L

P

K

L

H

E

K

P

V

Q

R

R

S

L

F

V

P

H

N

S

E

L

L

S

S

I

G

G

G

E

E

R

Q

Q

Q

R

R

V

I

E

A

I

I

V

A

R

R

C

A

L

I

M

V

Q

N

D

N

I

P

R

K

L

V

L

L

I

I

L

A

D

D

E

E

P

P

T

T

S

G

V

N

L

L

T

D

P

P

Q

D

E

N

A

S

D

W

E

E

L

I

F

M

V

N

T

L

L

L

R

E

R

R

L

I

A

N

A

L

E

Q

G

G

C

T

S

T

I

I

L

L

F

M

I

A

S

T

H

H

K

N

L

S

N

Q

E

I

V

V

R

N

A

T

L

L

C

R

Q

H

S

R

A

V

T

I

V

A

L

I

R

E

G

N

G

G

R

R

V

V

binding phosphoaminophosphonic acid-adenylate ester: Y13 (= Y18), G16 (= G20), A19 (= A23), S39 (≠ N43), G40 (= G44), G42 (= G46), K43 (= K47), S44 (= S48), T45 (= T49)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
31% identity, 45% coverage: 9:239/518 of query aligns to 1:229/367 of 1q12A

query
sites
1q12A

L

V

Q

Q

L

L

C

Q

G

N

I

V

T

T

K

K

Q

A

Y
x
W

P

G

G

E

C

V

L

V

A

V

N

S

D

K

R

D

I

I

D

N

L

L

S

D

I

I

Q

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

Q

T

P

I

D

T

A

S

G

G

A

D

I

L

H

F

W

I

Q

G

G

E

E

K

R

R

V

M

T

N

M

D

R

T

D

P

P

P

A

A

Q

-

A

-

R

-

E

E

R

R

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

I

M

A

S

L

F

A

G

L

L

G

K

A

L

K

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

P

A

L

H

E

L

P

L

Q

D

R
|
R

L

K

V

P

H

K

S
x
A

L

L

S
|
S

I

G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

V

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

N

V

E

E

V

A

R

M

A

T

L

L

C

A

Q

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

S

A

G

Q

E

V

C

G

I

K

P

P

A

L

E

E

C

L

S

Y

D

H

L

Y

E

P

L

A

A

D

R

R

L

F

M

V

V

A

G

G

binding adenosine-5'-triphosphate: W10 (≠ Y18), S35 (≠ N43), G36 (= G44), C37 (≠ A45), G38 (= G46), K39 (= K47), S40 (= S48), T41 (= T49), R126 (= R137), A130 (≠ S141), S132 (= S143), G134 (= G145), Q135 (≠ E146)

8k1pB Mycobacterial efflux pump, adp+vanadate bound state
32% identity, 41% coverage: 9:219/518 of query aligns to 4:207/213 of 8k1pB

query
sites
8k1pB

L

I

Q

E

L

I

C

R

G

G

I

L

T

T

K

K

Q

H

Y
x
F

P

G

G

S

C

V

L

R

A

A

N

L

D

D

R

G

I

L

D

D

L

L

S

T

I

V

Q

R

P

E

G

G

E

E

I

V

H

H

A

G

L

F

L

L

G

G

E

P

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

T

M

L

K

R

I

I

I

L

Y

L

G

G

V

L

T

V

Q

K

P

A

D

D

A

G

G

G

A

S

I

V

H

-

W

-

Q

-

G

-

E

-

R

R

V

L

T

L

M

G

R

G

D

D

P

P

-

W

-

T

-

D

A

A

Q

V

A

D

R

L

E

H

R

R

G

H

I

I

G

A

M

Y

V

V

F

P

Q

G

H

D

F

V

S

T

L

L

F

W

E

P

T

S

L

L

S

T

V

G

A

G

E

E

N

T

I

I

A

D

L

L

A

L

L

A

G

R

A

M

K

R

A

G

G

G

T

I

P

D

K

N

Q

-

L

-

E

-

P

A

K

R

I

R

R

A

E

E

V

L

S

I

Q

E

R

R

Y

F

G

G

M

-

P

-

L

L

E

D

P

P

Q

T

R

K

L

K

V

A

H

R

S
x
T

L

Y

S
|
S

I

K

G

G

E

N

R

R

Q

Q

R

K

V

V

E

S

I

L

V

I

R

S

C

A

L

L

M

S

Q

S

D

H

I

A

R

T

L

L

I

L

L

L

D

D

E

E

P

P

T

S

S

S

V

G

L

L

T

D

P

P

Q

L

E

M

A

E

D

N

E

V

L

F

F

Q

V

Q

T

C

L

I

R

G

R

E

L

A

A

R

A

Q

E

R

G

G

C

V

S

T

I

V

L

L

F

L

I

S

S

S

H

H

K

I

L

L

N

A

E

E

V

T

R

E

A

A

L

L

C

C

Q

E

S

K

A

V

T

T

V

I

L

I

R

R

G

A

G

G

R

K

V

T

binding adenosine-5'-diphosphate: F13 (≠ Y18), N38 (= N43), G39 (= G44), A40 (= A45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)
binding adenosine-5'-triphosphate: T129 (≠ S141), S131 (= S143)

8k1oB Mycobacterial efflux pump, amppnp bound state (see paper)
32% identity, 41% coverage: 9:219/518 of query aligns to 6:209/215 of 8k1oB

query
sites
8k1oB

L

I

Q

E

L

I

C

R

G

G

I

L

T

T

K

K

Q

H

Y

F

P

G

G

S

C

V

L

R

A

A

N

L

D

D

R

G

I

L

D

D

L

L

S

T

I

V

Q

R

P

E

G

G

E

E

I

V

H

H

A

G

L

F

L

L

G

G

E

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

T

M

L

K

R

I

I

I

L

Y

L

G

G

V

L

T

V

Q

K

P

A

D

D

A

G

G

G

A

S

I

V

H

-

W

-

Q

-

G

-

E

-

R

R

V

L

T

L

M

G

R

G

D

D

P

P

-

W

-

T

-

D

A

A

Q

V

A

D

R

L

E

H

R

R

G

H

I

I

G

A

M

Y

V

V

F

P

Q

G

H

D

F

V

S

T

L

L

F

W

E

P

T

S

L

L

S

T

V

G

A

G

E

E

N

T

I

I

A

D

L

L

A

L

L

A

G

R

A

M

K

R

A

G

G

G

T

I

P

D

K

N

Q

-

L

-

E

-

P

A

K

R

I

R

R

A

E

E

V

L

S

I

Q

E

R

R

Y

F

G

G

M

-

P

-

L

L

E

D

P

P

Q

T

R

K

L

K

V

A

H

R

S
x
T

L

Y

S
|
S

I

K

G

G

E

N

R

R

Q

Q

R

K

V

V

E

S

I

L

V

I

R

S

C

A

L

L

M

S

Q

S

D

H

I

A

R

T

L

L

I

L

L

L

D

D

E

E

P

P

T

S

S

S

V

G

L

L

T

D

P

P

Q

L

E

M

A

E

D

N

E

V

L

F

F

Q

V

Q

T

C

L

I

R

G

R

E

L

A

A

R

A

Q

E

R

G

G

C

V

S

T

I

V

L

L

F

L

I

S

S

S

H

H

K

I

L

L

N

A

E

E

V

T

R

E

A

A

L

L

C

C

Q

E

S

K

A

V

T

T

V

I

L

I

R

R

G

A

G

G

R

K

V

T

binding phosphoaminophosphonic acid-adenylate ester: N40 (= N43), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49), T131 (≠ S141), S133 (= S143)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
32% identity, 39% coverage: 27:228/518 of query aligns to 21:223/241 of 4u00A

query
sites
4u00A

I

I

D

H

L

L

S

E

I

V

Q

A

P

P

G

G

E

E

I

K

H

L

A

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

R

I

T

I

I

Y

N

G

R

V

L

T

E

Q

D

P

F

D

Q

A

E

G

G

A

E

I

V

H

V

W

V

Q

D

G

G

E

-

R

-

V

L

T

S

M

V

R

K

D

D

P

D

A

R

Q

A

A

L

R

R

E

E

-

I

-

R

R

R

G

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

Q

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

E

E

N

N

I

V

A

T

L

L

A

A

-

P

L

M

G

R

A

V

K

R

A

R

G

W

T

P

P

R

K

E

Q

K

L

A

E

E

P

K

K

K

I

A

R

L

E

E

V

L

S

L

Q

E

R

R

Y

V

G

G

M

I

P

L

L

D

E

Q

P

A

Q

R

R

K

L

Y

V

P

H

A

S

Q

L

L

S

S

I

G

G

G

E

Q

R

Q

Q

Q

R

R

V

V

E

A

I

I

V

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

D

T

V

L

M

R

R

R

D

L

L

A

A

A

Q

E

G

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

N

G

E

F

V

A

R

R

A

E

L

V

C

A

Q

D

S

R

A

V

T

V

V

F

L

M

R

D

G

G

R

Q

V

I

S

V

G

E

E

E

C

G

I

R

P

P

A

E

E

E

C

I

binding adenosine-5'-diphosphate: S37 (≠ N43), G38 (= G44), S39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 39% coverage: 27:230/518 of query aligns to 22:227/242 of 3c4jA

query
sites
3c4jA

I

I

D

N

L

V

S

H

I

I

Q

R

P

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

I

L

Y

N

G

L

V

L

T

E

Q

D

P

F

D

D

A

E

G

G

A

E

I

I

H

I

W

I

Q

D

G

G

E

I

R

N

V

L

T

K

M

A

R

K

D

D

P

T

A

N

Q

L

A

N

R

K

E

V

R

R

G

E

-

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

E

N

N

N

I

I

A

T

L

L

A

A

-

P

L

M

G

K

A

V

K

R

A

K

G

W

T

P

P

R

K

E

Q

K

L

A

E

E

P

A

K

K

I

A

R

M

E

E

V

L

S

L

Q

D

R

K

Y

V

G

G

M

L

P

K

L

D

E

K

P

A

Q

H

R

A

L

Y

V

P

H

D

S

S

L

L

S

S

I

G

G

G

E

Q

R

A

Q

Q

R

R

V

V

E

A

I

I

V

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

S

T

V

L

M

R

K

R

Q

L

L

A

A

A

N

E

E

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

N

G

E

F

V

A

R

R

A

E

L

V

C

G

Q

D

S

R

A

V

T

L

V

F

L

M

R

D

G

G

R

Y

V

I

S

I

G

E

E

E

C

G

I

K

P

P

A

E

E

D

C

L

S

F

D

D

binding phosphothiophosphoric acid-adenylate ester: S38 (≠ N43), G39 (= G44), S40 (≠ A45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13, 18

3c41J Abc protein artp in complex with amp-pnp/mg2+
30% identity, 39% coverage: 27:230/518 of query aligns to 22:227/242 of 3c41J

query
sites
3c41J

I

I

D

N

L

V

S

H

I

I

Q

R

P

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

I

L

Y

N

G

L

V

L

T

E

Q

D

P

F

D

D

A

E

G

G

A

E

I

I

H

I

W

I

Q

D

G

G

E

I

R

N

V

L

T

K

M

A

R

K

D

D

P

T

A

N

Q

L

A

N

R

K

E

V

R

R

G

E

-

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

E

N

N

N

I

I

A

T

L

L

A

A

-

P

L

M

G

K

A

V

K

R

A

K

G

W

T

P

P

R

K

E

Q

K

L

A

E

E

P

A

K

K

I

A

R

M

E

E

V

L

S

L

Q

D

R

K

Y

V

G

G

M

L

P

K

L

D

E

K

P

A

Q

H

R

A

L

Y

V

P

H

D

S

S

L

L

S

S

I

G

G

G

E

Q

R

A

Q

Q

R

R

V

V

E

A

I

I

V

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D
|
D

E

E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

S

T

V

L

M

R

K

R

Q

L

L

A

A

A

N

E

E

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

N

G

E

F

V

A

R

R

A

E

L

V

C

G

Q

D

S

R

A

V

T

L

V

F

L

M

R

D

G

G

R

Y

V

I

S

I

G

E

E

E

C

G

I

K

P

P

A

E

E

D

C

L

S

F

D

D

binding phosphoaminophosphonic acid-adenylate ester: S38 (≠ N43), G39 (= G44), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)
binding magnesium ion: S43 (= S48), D163 (= D166)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18

2olkA Abc protein artp in complex with adp-beta-s
30% identity, 39% coverage: 27:230/518 of query aligns to 22:227/242 of 2olkA

query
sites
2olkA

I

I

D

N

L

V

S

H

I

I

Q

R

P

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

I

L

Y

N

G

L

V

L

T

E

Q

D

P

F

D

D

A

E

G

G

A

E

I

I

H

I

W

I

Q

D

G

G

E

I

R

N

V

L

T

K

M

A

R

K

D

D

P

T

A

N

Q

L

A

N

R

K

E

V

R

R

G

E

-

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

E

N

N

N

I

I

A

T

L

L

A

A

-

P

L

M

G

K

A

V

K

R

A

K

G

W

T

P

P

R

K

E

Q

K

L

A

E

E

P

A

K

K

I

A

R

M

E

E

V

L

S

L

Q

D

R

K

Y

V

G

G

M

L

P

K

L

D

E

K

P

A

Q

H

R

A

L

Y

V

P

H

D

S

S

L

L

S

S

I

G

G

G

E

Q

R

A

Q

Q

R

R

V

V

E

A

I

I

V

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

S

T

V

L

M

R

K

R

Q

L

L

A

A

A

N

E

E

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

N

G

E

F

V

A

R

R

A

E

L

V

C

G

Q

D

S

R

A

V

T

L

V

F

L

M

R

D

G

G

R

Y

V

I

S

I

G

E

E

E

C

G

I

K

P

P

A

E

E

D

C

L

S

F

D

D

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S38 (≠ N43), G39 (= G44), S40 (≠ A45), G41 (= G46), K42 (= K47), S43 (= S48), T44 (= T49)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13, 18

Query Sequence

>WP_041755622.1 NCBI__GCF_000013785.1:WP_041755622.1
MPESQTARLQLCGITKQYPGCLANDRIDLSIQPGEIHALLGENGAGKSTLMKIIYGVTQP
DAGAIHWQGERVTMRDPAQARERGIGMVFQHFSLFETLSVAENIALALGAKAGTPKQLEP
KIREVSQRYGMPLEPQRLVHSLSIGERQRVEIVRCLMQDIRLLILDEPTSVLTPQEADEL
FVTLRRLAAEGCSILFISHKLNEVRALCQSATVLRGGRVSGECIPAECSDLELARLMVGD
AEGLEAEYPKSEGRAPFLRVERLSWHNADPFGVSLEEVDLEVRAGEIVGIAGVAGNGQDE
LLALLSGEQRLPAAQAMRIRFLGDDVAHLRPDARRRHGMAFVPAERLGHGAVPSMSLADN
GLLTAFQQTGMVERGLIRRGKVRAFAEQVIQRFAVKTPDAQTPAASLSGGNLQKFILGRE
ILQQPKLLIAAHPTWGVDVGAAAAIHRALIELRDAGAAILVISEDLEELFQISDRIAALS
DGRLSPQRATASTCPVEVGRWMAGQFDTSDDPVSTSAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory