SitesBLAST

R49 (= R45) mutation to A: Less than 1% of the wild-type enzyme activity.
K60 (= K56) mutation to A: Less than 1% of the wild-type enzyme activity.
D69 (= D65) mutation to A: No effect on the enzyme activity.
R72 (= R68) mutation to A: Less than 1% of the wild-type enzyme activity.
T105 (≠ S101) mutation to A: 20% of the wild-type enzyme activity.
E109 (≠ Q105) mutation to A: 10% of the wild-type enzyme activity.; mutation to Q: 40% of the wild-type enzyme activity at pH 7.5 and 27% of the wild-type enzyme activity at pH 4.
R134 (= R130) mutation to A: Less than 1% of the wild-type enzyme activity.
C160 (= C156) modified: Disulfide link with 193
C193 (= C189) modified: Disulfide link with 160

Sites not aligning to the query:

1:33 signal peptide

2ao2B The 2.07 angstrom crystal structure of mycobacterium tuberculosis chorismate mutase reveals unexpected gene duplication and suggests a role in host-pathogen interactions (see paper)
28% identity, 80% coverage: 33:191/198 of query aligns to 3:161/165 of 2ao2B

query
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2ao2B

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active site: R15 (= R45), K26 (= K56), D35 (= D65), T71 (≠ S101), E72 (≠ K102), E75 (≠ Q105), R100 (= R130)
binding sulfate ion: K26 (= K56), R38 (= R68), Q42 (≠ L72)
binding tryptophan: L31 (≠ Q61), P32 (= P62), I33 (= I63), E34 (≠ T64)

2ao2A The 2.07 angstrom crystal structure of mycobacterium tuberculosis chorismate mutase reveals unexpected gene duplication and suggests a role in host-pathogen interactions (see paper)
28% identity, 80% coverage: 33:191/198 of query aligns to 3:161/165 of 2ao2A

query
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2ao2A

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active site: R15 (= R45), K26 (= K56), D35 (= D65), T71 (≠ S101), E72 (≠ K102), E75 (≠ Q105), R100 (= R130)
binding sulfate ion: K26 (= K56), R38 (= R68), R100 (= R130)

2fp2B Secreted chorismate mutase from mycobacterium tuberculosis (see paper)
28% identity, 80% coverage: 33:191/198 of query aligns to 1:159/163 of 2fp2B

query
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active site: R13 (= R45), K24 (= K56), D33 (= D65), T69 (≠ S101), E70 (≠ K102), E73 (≠ Q105), R98 (= R130)
binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R13 (= R45), K24 (= K56), I31 (= I63), E32 (≠ T64), D33 (= D65), R36 (= R68), Q40 (≠ L72), I66 (= I98), T69 (≠ S101), E70 (≠ K102), E73 (≠ Q105), R98 (= R130)

Q7CHH5 Secreted chorismate mutase; CM; *YpCM; EC 5.4.99.5 from Yersinia pestis (see paper)
25% identity, 54% coverage: 41:146/198 of query aligns to 39:143/186 of Q7CHH5

query
sites
Q7CHH5

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Sites not aligning to the query:

33 modified: Disulfide link with 148
148 modified: Disulfide link with 33

Query Sequence

>WP_041763696.1 NCBI__GCF_000020045.1:WP_041763696.1
MTRPNLIGALGAILLCSTLAFAPAPARADGDDTSLTNLVALVSQRLALAEPVARYKWAHH
QPITDTPREQALLTDVEKRASRAGVDPAFAHAFFQDQIDASKDVQNALFDTWRTTRPPEG
AAPDLATSLRPQLDKLTQSLIPALARVQRLRAQDDCPMRVARSIDNWKSLTRYDNTHNAA
LTRALGHVCESGGVGATG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory