SitesBLAST
Comparing WP_041937022.1 NCBI__GCF_000009265.1:WP_041937022.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 16 hits to proteins with known functional sites (download)
P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
22% identity, 79% coverage: 14:361/438 of query aligns to 76:428/534 of P53322
- K283 (≠ D223) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
8u3hA Structure of fmoc-leu-oh bound sialin
22% identity, 89% coverage: 38:426/438 of query aligns to 15:409/425 of 8u3hA
8u3gA Structure of naag-bound sialin
22% identity, 89% coverage: 38:426/438 of query aligns to 15:411/427 of 8u3gA
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
23% identity, 90% coverage: 34:427/438 of query aligns to 21:418/430 of P0AA76
- Y29 (= Y42) binding D-galactonate
- D31 (= D44) mutation to N: Loss of galactonate transport activity.
- R32 (= R45) binding D-galactonate
- Y64 (= Y77) binding D-galactonate
- E118 (= E131) mutation to Q: Loss of galactonate transport activity.
- W358 (= W366) binding D-galactonate
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
23% identity, 90% coverage: 34:427/438 of query aligns to 10:399/409 of 6e9nA
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
22% identity, 81% coverage: 71:426/438 of query aligns to 113:472/495 of Q9NRA2
- K136 (≠ R94) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ I139) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ II 154:155) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ LT 229:230) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSLRN 268:272 (≠ ANIEA 231:235) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (= G293) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (= P299) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (vs. gap) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
23% identity, 90% coverage: 34:427/438 of query aligns to 13:383/393 of 6e9oA
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 83% coverage: 71:434/438 of query aligns to 113:479/495 of Q8BN82
- H183 (≠ I139) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 71% coverage: 122:434/438 of query aligns to 166:479/495 of Q5Q0U0
- R168 (= R124) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- E171 (≠ L127) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
- G172 (= G128) mutation to C: Decreases protein levels and alters subcellular localization.
- E175 (= E131) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
- G176 (≠ A132) mutation to C: Decreases protein levels and alters subcellular localization.
- F179 (= F135) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
- P180 (= P136) mutation to C: Decreases protein levels and alters subcellular localization.
- H183 (≠ I139) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- W186 (≠ L142) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- SSLKN 268:272 (≠ ANIEA 231:235) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
- P334 (= P299) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- G371 (vs. gap) mutation to V: Remains in the endoplasmic reticulum.
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
- 136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.
Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
25% identity, 81% coverage: 73:426/438 of query aligns to 90:455/493 of Q03567
Sites not aligning to the query:
- 69 modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 87% coverage: 40:422/438 of query aligns to 93:486/582 of Q9JI12
- H128 (≠ A70) mutation to A: Greatly lowers L-glutamate transport.
- R184 (= R124) mutation R->A,E,K: Greatly lowers L-glutamate transport.
- E191 (= E131) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
- R322 (≠ F266) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.
Sites not aligning to the query:
- 88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
22% identity, 87% coverage: 40:422/438 of query aligns to 35:428/452 of 7t3nA
Sites not aligning to the query:
Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
21% identity, 87% coverage: 18:396/438 of query aligns to 18:404/448 of Q51955
- D41 (= D44) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- D44 (≠ N47) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- G85 (≠ N85) mutation to V: Abolishes 4-HBA transport and chemotaxis.
- D89 (≠ N89) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- G92 (= G92) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
- R124 (= R124) mutation to A: Abolishes 4-HBA transport.
- E144 (≠ A144) mutation to A: Strong decrease in 4-HBA transport.
- H183 (≠ Q190) mutation to A: Decrease in 4-HBA transport and chemotaxis.
- D323 (= D315) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- H328 (= H323) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.
- R386 (≠ A378) mutation to A: Strong decrease in 4-HBA transport.
- R398 (≠ N390) mutation to A: Abolishes 4-HBA transport.
Sites not aligning to the query:
- 444 H→A: No change in 4-HBA transport and chemotaxis.
Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
23% identity, 68% coverage: 18:314/438 of query aligns to 86:394/570 of Q9C0U9
Sites not aligning to the query:
- 14 modified: Phosphoserine
Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
22% identity, 65% coverage: 3:285/438 of query aligns to 11:283/444 of Q8NLB7
- D54 (vs. gap) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- D57 (= D44) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- R103 (≠ K90) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 309 W→V: Loss of transport activity.
- 312 D→A: Loss of transport activity.
- 313 R→A: Loss of transport activity.
- 317 mutation I->H,Y: Loss of transport activity.
- 386 R→A: Loss of transport activity.
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 88% coverage: 12:396/438 of query aligns to 5:395/452 of Q5EXK5
- D82 (≠ N89) mutation to A: Loss of activity.
- V311 (≠ R312) mutation to W: Loss of activity.
- D314 (= D315) mutation to A: Loss of activity.
Query Sequence
>WP_041937022.1 NCBI__GCF_000009265.1:WP_041937022.1
MTLQTQAPVVGLHPAQTLEDRAYGKVFWRIVPFLMLCYVVAYLDRVNVGFAKLQMSSELG
LSEAAYGIGAGIFFIGYFLFEVPSNIIMNKVGARVWIARIMVTWGIISAALMFTSSETVF
YVLRFLLGVAEAGFFPGIILYLTAWYPAHRRARIITTFMSAIPISAIFGNPLSGLLMDSF
HGTHGLSGWQWMFLIEAIPAILFGVATFFYLDDTIQGAKWLNDEEKRVLTANIEAENRAK
TASPHSIGATLTDRRVWLMCLIYFCFVLGQYGLNFWMPTIVKASGVNGNLNIGLISAIPY
ICTFVVMLALGRSADRLRERRWHLVVPAFIAAGGFVAATMATSTTVSIVCLSLAAAGAIS
CAPLFWSLPTAFLAGTGAAAGIAWINSVGNLAGFLGPFLVGYLKDFTGTNSAGMYLLAAA
LVIGSLAVLTVPAKSVNR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory