SitesBLAST
Comparing WP_041938687.1 NCBI__GCF_000058485.1:WP_041938687.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3rwbA Crystal structure of complex of 4pal (4-pyridoxolactone) and pldh (tetrameric pyridoxal 4-dehydrogenase) from mesorhizobium loti
42% identity, 98% coverage: 3:250/252 of query aligns to 5:247/247 of 3rwbA
- active site: G17 (= G15), S140 (= S145), Y153 (= Y158), K157 (= K162)
- binding 7-hydroxy-6-methylfuro[3,4-c]pyridin-1(3H)-one: S140 (= S145), N141 (≠ G146), T142 (≠ S147), M150 (≠ Q155), Y153 (= Y158), L185 (= L190), H196 (≠ P201)
- binding nicotinamide-adenine-dinucleotide: G13 (= G11), Q16 (≠ G14), G17 (= G15), I18 (= I16), D37 (= D35), I38 (≠ V36), D60 (= D66), I61 (= I67), N87 (= N93), A88 (= A94), S89 (≠ G95), I138 (= I143), S140 (= S145), Y153 (= Y158), K157 (= K162), P183 (= P188), L185 (= L190), I186 (≠ T191), S188 (≠ T193), G190 (≠ A195), V191 (≠ A196)
3ndrA Crystal structure of tetrameric pyridoxal 4-dehydrogenase from mesorhizobium loti
42% identity, 98% coverage: 3:250/252 of query aligns to 5:247/247 of 3ndrA
- active site: G17 (= G15), S140 (= S145), Y153 (= Y158), K157 (= K162)
- binding nicotinamide-adenine-dinucleotide: G13 (= G11), Q16 (≠ G14), G17 (= G15), I18 (= I16), D37 (= D35), I38 (≠ V36), D60 (= D66), I61 (= I67), N87 (= N93), A88 (= A94), S89 (≠ G95), V110 (= V116), I138 (= I143), S140 (= S145), Y153 (= Y158), K157 (= K162), P183 (= P188), L185 (= L190), I186 (≠ T191), S188 (≠ T193), G190 (≠ A195), V191 (≠ A196)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
39% identity, 98% coverage: 3:248/252 of query aligns to 4:247/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), Q15 (≠ G14), G16 (= G15), I17 (= I16), D36 (= D35), V63 (≠ I67), N89 (= N93), A91 (≠ G95), S94 (vs. gap), I142 (= I143), S143 (≠ G144), S144 (= S145), Y157 (= Y158), K161 (= K162), P187 (= P188), H188 (≠ G189), I190 (≠ T191), I194 (≠ A195)
Q5P5I4 (S)-1-Phenylethanol dehydrogenase; EC 1.1.1.311 from Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) (see 2 papers)
38% identity, 98% coverage: 5:250/252 of query aligns to 8:249/249 of Q5P5I4
- NGI 17:19 (≠ GGI 14:16) binding NAD(+)
- D38 (= D35) binding NAD(+)
- CDV 61:63 (≠ TDI 65:67) binding NAD(+)
- N89 (= N93) binding NAD(+)
- Y93 (≠ F97) binding NAD(+)
- K158 (= K162) binding NAD(+)
- PSLV 184:187 (≠ PGL- 188:190) binding NAD(+)
- T191 (= T193) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2ewmB Crystal structure of the (s)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain ebn1 (see paper)
38% identity, 98% coverage: 5:250/252 of query aligns to 6:247/247 of 2ewmB
- active site: G16 (= G15), S139 (= S145), Y149 (≠ Q155), Y152 (= Y158), K156 (= K162)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), N15 (≠ G14), G16 (= G15), I17 (= I16), D36 (= D35), L37 (≠ V36), C59 (≠ T65), D60 (= D66), V61 (≠ I67), N87 (= N93), S139 (= S145), Y152 (= Y158), K156 (= K162), P182 (= P188), S183 (≠ G189), L184 (= L190), V185 (vs. gap), T189 (= T193)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
35% identity, 97% coverage: 5:248/252 of query aligns to 8:241/244 of 4nbuB
- active site: G18 (= G15), N111 (= N117), S139 (= S145), Q149 (= Q155), Y152 (= Y158), K156 (= K162)
- binding acetoacetyl-coenzyme a: D93 (≠ Q99), K98 (≠ E104), S139 (= S145), N146 (≠ T152), V147 (≠ P153), Q149 (= Q155), Y152 (= Y158), F184 (≠ L190), M189 (≠ A195), K200 (≠ E207)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G11), N17 (≠ G14), G18 (= G15), I19 (= I16), D38 (= D35), F39 (≠ V36), V59 (≠ T65), D60 (= D66), V61 (≠ I67), N87 (= N93), A88 (= A94), G89 (= G95), I90 (≠ R96), T137 (≠ I143), S139 (= S145), Y152 (= Y158), K156 (= K162), P182 (= P188), F184 (≠ L190), T185 (= T191), T187 (= T193), M189 (≠ A195)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
39% identity, 98% coverage: 5:252/252 of query aligns to 6:246/248 of Q9KJF1
- S15 (≠ G14) binding NAD(+)
- D36 (= D35) binding NAD(+)
- D62 (= D66) binding NAD(+)
- I63 (= I67) binding NAD(+)
- N89 (= N93) binding NAD(+)
- Y153 (= Y158) binding NAD(+)
- K157 (= K162) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
39% identity, 98% coverage: 5:252/252 of query aligns to 5:245/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), M16 (≠ I16), D35 (= D35), I36 (≠ V36), I62 (= I67), N88 (= N93), G90 (= G95), I138 (= I143), S140 (= S145), Y152 (= Y158), K156 (= K162), I185 (≠ T191)
3uf0A Crystal structure of a putative NAD(p) dependent gluconate 5- dehydrogenase from beutenbergia cavernae(efi target efi-502044) with bound NADP (low occupancy)
41% identity, 98% coverage: 3:248/252 of query aligns to 6:245/249 of 3uf0A
- active site: G18 (= G15), S141 (= S145), V151 (≠ Q155), Y154 (= Y158), K158 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G11), S17 (≠ G14), G18 (= G15), I19 (= I16), R39 (≠ D29), D63 (= D66), L64 (≠ I67), N89 (= N93), G91 (= G95), I92 (≠ R96), I139 (= I143), A140 (≠ G144), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (= P188), G185 (= G189), V187 (≠ T191), T189 (= T193), N191 (≠ A195), T192 (≠ A196)
3o03A Quaternary complex structure of gluconate 5-dehydrogenase from streptococcus suis type 2 (see paper)
35% identity, 97% coverage: 5:248/252 of query aligns to 12:245/254 of 3o03A
- active site: G22 (= G15), S147 (= S145), V157 (≠ Q155), Y160 (= Y158), K164 (= K162)
- binding calcium ion: S147 (= S145), M148 (≠ G146), P190 (= P188)
- binding D-gluconic acid: I99 (≠ F97), R101 (≠ Q99), S147 (= S145), M149 (≠ S147), R154 (≠ T152), Y160 (= Y158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G11), Y21 (≠ G14), G22 (= G15), I23 (= I16), D42 (= D35), I43 (≠ V36), L47 (≠ A40), D68 (= D66), V69 (≠ I67), N95 (= N93), A96 (= A94), G97 (= G95), I145 (= I143), Y160 (= Y158), K164 (= K162), P190 (= P188)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
39% identity, 97% coverage: 5:248/252 of query aligns to 7:248/252 of 1vl8B
- active site: G17 (= G15), S143 (= S145), I154 (≠ Q155), Y157 (= Y158), K161 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), R16 (≠ G14), G17 (= G15), L18 (≠ I16), S37 (≠ D35), R38 (≠ V36), C63 (vs. gap), D64 (= D66), V65 (≠ I67), A91 (≠ N93), A92 (= A94), G93 (= G95), I94 (≠ R96), V114 (= V116), I141 (= I143), S143 (= S145), Y157 (= Y158), K161 (= K162), P187 (= P188), G188 (= G189), Y190 (≠ T191), T192 (= T193), M194 (≠ A195), T195 (≠ A196)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
37% identity, 97% coverage: 4:248/252 of query aligns to 6:243/247 of P73574
- A14 (= A12) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P153) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K162) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ L190) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ L200) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
5thqB Comprehensive analysis of a novel ketoreductase for pentangular polyphenol biosynthesis (see paper)
41% identity, 77% coverage: 54:248/252 of query aligns to 56:248/250 of 5thqB
- active site: S142 (= S145), R152 (≠ Q155), Y155 (= Y158), K159 (= K162)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A64 (≠ T65), L66 (≠ I67), N92 (= N93), A93 (= A94), V95 (≠ R96), I140 (= I143), S142 (= S145), Y155 (= Y158), K159 (= K162), G186 (= G189), V187 (≠ L190), V188 (≠ T191)
Sites not aligning to the query:
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
35% identity, 98% coverage: 5:252/252 of query aligns to 6:244/244 of 6t77A
- active site: G16 (= G15), S138 (= S145), Y151 (= Y158)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (= S13), R15 (≠ G14), T37 (≠ V36), L58 (≠ A62), N59 (≠ V63), V60 (≠ A64), A87 (= A94), G88 (= G95), I89 (≠ R96)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
34% identity, 98% coverage: 5:251/252 of query aligns to 6:239/239 of 4nbtA
- active site: G16 (= G15), S132 (= S145), Y145 (= Y158), K149 (= K162)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), K15 (≠ G14), G16 (= G15), L17 (≠ I16), D36 (= D35), L37 (≠ V36), L52 (≠ T65), N53 (≠ D66), V54 (≠ I67), N80 (= N93), A81 (= A94), G82 (= G95), I130 (= I143), S132 (= S145), Y145 (= Y158), K149 (= K162), P177 (= P188), G178 (= G189), I180 (≠ T191), T182 (= T193)
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
36% identity, 97% coverage: 5:248/252 of query aligns to 6:240/251 of 6vspA
- active site: G16 (= G15), S138 (= S145), Y151 (= Y158)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), N15 (≠ G14), G16 (= G15), M17 (≠ I16), D36 (= D35), W37 (≠ V36), W37 (≠ V36), A38 (≠ S37), I59 (≠ T65), D60 (= D66), V61 (≠ I67), N87 (= N93), A88 (= A94), G89 (= G95), V90 (≠ R96), V110 (= V116), T136 (≠ I143), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (= P188), S182 (≠ G189), L183 (= L190), V184 (≠ T191), T186 (= T193), N187 (≠ P194), M188 (≠ A195), T189 (≠ A196)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
36% identity, 97% coverage: 5:248/252 of query aligns to 6:240/251 of H9XP47
- N15 (≠ G14) binding NAD(+)
- M17 (≠ I16) binding NAD(+)
- D36 (= D35) binding NAD(+)
- D60 (= D66) binding NAD(+)
- V61 (≠ I67) binding NAD(+)
- N87 (= N93) binding NAD(+)
- S138 (= S145) binding (R)-acetoin; binding (S)-acetoin
- V139 (≠ G146) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (= S147) binding (R)-acetoin
- Y151 (= Y158) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
- K155 (= K162) binding NAD(+)
- V184 (≠ T191) binding NAD(+)
- T186 (= T193) binding NAD(+)
- RDK 197:199 (≠ LAE 205:207) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
36% identity, 97% coverage: 5:248/252 of query aligns to 6:240/251 of 6xewA
- active site: G16 (= G15), S138 (= S145), Y151 (= Y158)
- binding r,3-hydroxybutan-2-one: S138 (= S145), S140 (= S147), Y151 (= Y158)
- binding s,3-hydroxybutan-2-one: S138 (= S145), Y151 (= Y158), S182 (≠ G189)
- binding nicotinamide-adenine-dinucleotide: G12 (= G11), N15 (≠ G14), G16 (= G15), M17 (≠ I16), D36 (= D35), W37 (≠ V36), W37 (≠ V36), A38 (≠ S37), I59 (≠ T65), D60 (= D66), V61 (≠ I67), N87 (= N93), A88 (= A94), G89 (= G95), V110 (= V116), T136 (≠ I143), S138 (= S145), Y151 (= Y158), K155 (= K162), S182 (≠ G189), L183 (= L190), V184 (≠ T191), T186 (= T193), N187 (≠ P194), M188 (≠ A195), T189 (≠ A196)
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
36% identity, 97% coverage: 5:248/252 of query aligns to 8:242/252 of 6vspB
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
34% identity, 100% coverage: 1:251/252 of query aligns to 1:246/246 of 3osuA
Query Sequence
>WP_041938687.1 NCBI__GCF_000058485.1:WP_041938687.1
MSAARTVVVTGASGGIGSEIVNRFLAHGDTVVAADVSQEALDTWRARWDSGAPGGRHPSL
HAVATDIASEESVAALVQVVQQSLGTVDVLINNAGRFPQTAFEEMSTDEWRQVIDVNLTG
TFLMIRAFVPLLKASGRGRVVNIGSGSVFSGTPMQSHYVASKGGVLGLTRVLARELGGYG
ITVNVITPGLTVTPAAAAVLPEALLAEQRDARALHRDETPEDLVGPIFFLASDDAAFVTG
QTLNVDGGRHLL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory