SitesBLAST

S13 (= S13) binding FAD
E32 (≠ D32) binding FAD
R33 (= R33) mutation to E: Slight decrease of affinity for p-OHB and strong decrease of affinity for NADPH.; mutation to K: Slight decrease of affinity for p-OHB and NADPH.; mutation to S: Slight decrease of affinity for p-OHB and strong decrease of affinity for NADPH.
Q34 (≠ V34) mutation to K: Slight decrease of affinity for p-OHB and NADPH.; mutation to R: Slight decrease of affinity for p-OHB and NADPH.; mutation to T: Slight decrease of affinity for p-OHB and NADPH.
Y38 (= Y38) mutation to E: Slight decrease of affinity for p-OHB and strong decrease of affinity for NADPH.; mutation to F: Slight decrease of affinity for p-OHB and strong decrease of affinity for NADPH.; mutation to K: Slight decrease of affinity for p-OHB and strong decrease of affinity for NADPH.
R42 (= R42) mutation to K: 4-fold and 10-fold decrease of affinity for p-OHB and NADPH, respectively. The turnover rate of p-hydroxybenzoate hydroxylase results from impaired binding of NADPH.; mutation to S: 3-fold and 10-fold decrease of affinity for p-OHB and NADPH, respectively. The turnover rate of p-hydroxybenzoate hydroxylase results from impaired binding of NADPH. Hardly disturbs the binding of FAD.
RIRAGV 42:47 (≠ RVRAGV 42:47) binding FAD
R44 (= R44) mutation to K: Decrease of affinity for the flavin prosthetic group. It affects NADPH binding, resulting in a low yield of the charge-transfer species between reduced flavin and NADP.
Q102 (= Q101) binding FAD
C116 (= C115) mutation to S: Slight decrease of affinity for NADPH and p-OHB are observed.
F161 (= F160) mutation to A: Decrease of affinity for NADPH.; mutation to G: Decrease of affinity for NADPH.
H162 (= H161) mutation to D: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.; mutation to K: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases slightly.; mutation to N: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.; mutation to R: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases slightly.; mutation to S: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.; mutation to T: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.; mutation to Y: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases slightly.
R166 (= R165) mutation to E: Loses the ability to bind NADPH and FAD.; mutation to K: Loses the ability to bind NADPH.; mutation to S: Loses the ability to bind NADPH.
Y201 (= Y200) binding substrate
SQR 212:214 (≠ SMR 211:213) binding substrate
R214 (= R213) mutation to K: Strong decrease of affinity for NADPH and 4-fold decrease of affinity for p-OHB are observed.
Y222 (= Y221) binding substrate; mutation to A: Results in the removal of a large side chain involving in the binding of the carboxyl group of the substrate.
R269 (= R268) mutation to D: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.; mutation to K: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases slightly.; mutation to N: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.; mutation to S: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases slightly.; mutation to T: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.; mutation to Y: No significant changes in affinity for p-OHB are observed. However, the affinity for NADPH decreases strongly.
D286 (= D285) binding FAD
P293 (= P292) binding substrate
LN 299:300 (= LN 298:299) binding FAD

2phhA The coenzyme analogue adenosine 5-diphosphoribose displaces fad in the active site of p-hydroxybenzoate hydroxylase. An x-ray crystallographic investigation (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/391 of 2phhA

query
sites
2phhA

M

M

R

K

T

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Q

Q

V

V

A

A

I

I

I
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I

G

G

S

A

G

G

P
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P

S
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S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
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R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R
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R

V

I

R
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R

A

A

G

G

V

V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

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M

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A

A

R

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D

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G

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H

D

E

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G

F

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I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
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Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

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A

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E

A

A

C

C

G

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D

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A

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H

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D

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D

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Y

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K

R

D

D

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D

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D

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Y

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Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding adenosine-5-diphosphoribose: I8 (= I8), P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), R44 (= R44), Q102 (= Q101), D159 (= D158), I164 (≠ T163), G285 (= G284), D286 (= D285), G298 (= G297), L299 (= L298)

1pdhA Crystal structure of p-hydroxybenzoate hydroxylase reconstituted with the modified fad present in alcohol oxidase from methylotrophic yeasts: evidence for an arabinoflavin (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/391 of 1pdhA

query
sites
1pdhA

M

M

R

K

T

T

Q

Q

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V

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A

I

I

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S

G

G

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L

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G

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Q

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K

A

A

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G

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D

N

N

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L

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N

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M

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A

K

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A

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A

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D

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L

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M

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D

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G

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Y

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G

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L

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A

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D

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A

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S

N

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L

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Y

A

A

N

N

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P

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R

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G

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F

A

A

L

L

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C

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M

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Y

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Y
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Y

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L

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K

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A

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H

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P
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P

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T

G

G

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A

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K

G
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G

L

L

N

N

L

L

A

A

S

S

D

D

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V

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S

Y

T

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L

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Y

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G

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Y

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E

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C

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K

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M

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D

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E

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G

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Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding arabino-flavin-adenine dinucleotide: P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), R44 (= R44), A45 (= A45), Q102 (= Q101), D159 (= D158), Y222 (= Y221), D286 (= D285), P293 (= P292), G298 (= G297)

1iusA P-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate at ph 5.0 (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/394 of 1iusA

query
sites
1iusA

M

M

R

K

T

T

Q

Q

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A

A

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I

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G

P
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S
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S

G

G

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L

G

G

Q

Q

L

L

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H

E

K

A

A

G

G

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I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
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R

V

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P

N

D

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Y

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L

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R

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R

A
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A

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V

L

L

E

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M

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D

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L

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A

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G

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D

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A

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D

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E

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L

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Y
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Y

A

A

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N

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H

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P

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R

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G

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F

A

A

L
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L

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C

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S

M

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R

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S

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A

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T

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S

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Y

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Y
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Y

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K

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D
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D

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A

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H

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P

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T

G

G

A
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A

K
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K

G
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G

L
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L

N
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N

L

L

A

A

S

S

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D

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K

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Y

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E

A

R

Y

Y

S

S

Q

A

T

I

A

C

L

L

T

R

R

R

V

I

W

W

K

K

A

A

V

E

R

R

F

F

S

S

W

W

M

M

T

T

T

S

M

V

M

L

H

H

R

R

F

F

P

P

D

D

T

T

S

D

D

A

F

F

D

S

Q

Q

K

R

I

I

Q

Q

E

Q

A

T

E

E

L

L

D

E

Y

Y

L

Y

T

L

H

G

S

S

R

E

A

A

A

G

S

L

M

A

V

T

L

I

A

A

E

E

N

N

Y
|
Y

V

V

G

G

L

L

P

P

F

Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding flavin-adenine dinucleotide: G11 (= G11), P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), R44 (= R44), A45 (= A45), V47 (= V47), Q102 (= Q101), D159 (= D158), D286 (= D285), A296 (= A295), K297 (= K296), G298 (= G297), L299 (= L298), N300 (= N299)
binding 4-aminobenzoic acid: Y201 (= Y200), L210 (= L209), S212 (= S211), R214 (= R213), Y222 (= Y221), P293 (= P292)

1dodA The mobil flavin of 4-oh benzoate hydroxylase: motion of a prosthetic group regulates catalysis (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/394 of 1dodA

query
sites
1dodA

M

M

R

K

T

T

Q

Q

V

V

A

A

I

I

G

G

S

A

G

G

P
|
P

S
|
S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
|
R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R
|
R

V

I

R
|
R

A
|
A

G

G

V
|
V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

L

M

H

A

A

R

E

D

G

G

L

L

P

V

H
|
H

D

E

G

G

F

V

S

E

L

I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
|
Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

R

A

R

R

E

E

A

A

C

C

G

G

S

A

L

T

S

T

I

V

Y

Y

D

Q

A

A

D

E

V

V

A

R

P

L

H

H

D

D

V

L

D

Q

G

G

G

E

S

R

P

P

F

Y

V

V

T

T

Y

F

T

E

K

R

D

D

G

G

I

E

G

R

H

L

R

R

I

L

D

D

C

C

D

D

F

Y

I

I

A

A

G

G

C

C

D
|
D

G

G

F

F

H

H

G

G

T

I

S

S

R

R

R

Q

A

S

L

I

P

P

E

A

G

E

A

R

V

L

R

K

S

V

F

F

E

E

K

R

V

V

Y

Y

P

P

F

F

G

G

W

W

L

L

G

G

L

L

A

A

D

D

V

T

A

P

P

P

V

V

S

S

N

H

E

E

L

L

I

I

Y
|
Y

A

A

N

N

H

H

P

P

R

R

G

G

F

F

A

A

L

L

C

C

S
|
S

M

Q

R
|
R

S

S

A

A

T

T

R

R

S

S

R

R

Y

Y

Y
|
Y

I

V

Q

Q

C

V

P

P

L

L

D

S

A

E

K

K

I

V

E

E

D

D

W

W

S

S

D

D

D

E

R

R

F

F

W

W

D

T

E

E

L

L

R

K

R

A

R

R

L

L

P

P

A

S

H

E

H

V

A

A

E

E

A

K

L

L

T

V

T

T

A

G

P

P

S

S

F

L

E

E

K

K

S

S

I

I

A

A

P

P

L

L

R

R

S

S

F

F

V

V

T

V

E

E

P

P

M

M

R

Q

F

H

G

G

R

R

L

L

F

F

L

L

V

A

G

G

D
|
D

A

A

H

H

I

I

V

V

P

P

P
|
P

T
|
T

G

G

A
|
A

K
|
K

G
|
G

L

L

N

N

L

L

A

A

S

S

D

D

V

V

H

S

Y

T

L

L

F

Y

G

R

G

L

L

L

E

K

H

A

Y

Y

R

R

E

E

G

G

S

R

D

G

S

E

G

L

I

L

D

E

A

R

Y

Y

S

S

Q

A

T

I

A

C

L

L

T

R

R

R

V

I

W

W

K

K

A

A

V

E

R

R

F

F

S

S

W

W

M

M

T

T

T

S

M

V

M

L

H

H

R

R

F

F

P

P

D

D

T

T

S

D

D

A

F

F

D

S

Q

Q

K

R

I

I

Q

Q

E

Q

A

T

E

E

L

L

D

E

Y

Y

L

Y

T

L

H

G

S

S

R

E

A

A

A

G

S

L

M

A

V

T

L

I

A

A

E

E

N

N

Y
|
Y

V

V

G

G

L

L

P

P

F

Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding 2,4-dihydroxybenzoic acid: V47 (= V47), Y201 (= Y200), S212 (= S211), R214 (= R213), Y222 (= Y221), P293 (= P292), T294 (= T293), A296 (= A295)
binding flavin-adenine dinucleotide: P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), R44 (= R44), A45 (= A45), Q102 (= Q101), D159 (= D158), Y222 (= Y221), D286 (= D285), P293 (= P292), G298 (= G297)

1d7lA Structure-function correlations of the reaction of reduced nicotinamide analogs with p-hydroxybenzoate hydroxylase substituted with a series of 8-substituted flavins (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/394 of 1d7lA

query
sites
1d7lA

M

M

R

K

T

T

Q

Q

V

V

A

A

I

I

G
|
G

S

A

G
|
G

P
|
P

S
|
S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
|
R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R
|
R

V

I

R
|
R

A
|
A

G
|
G

V
|
V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

L

M

H

A

A

R

E

D

G

G

L

L

P

V

H
|
H

D

E

G

G

F

V

S

E

L

I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
|
Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

R

A

R

R

E

E

A

A

C

C

G

G

S

A

L

T

S

T

I

V

Y

Y

D

Q

A

A

D

E

V

V

A

R

P

L

H

H

D

D

V

L

D

Q

G

G

G

E

S

R

P

P

F

Y

V

V

T

T

Y

F

T

E

K

R

D

D

G

G

I

E

G

R

H

L

R

R

I

L

D

D

C

C

D

D

F

Y

I

I

A

A

G

G

C

C

D
|
D

G

G

F

F

H

H

G

G

T
x
I

S

S

R

R

R

Q

A

S

L

I

P

P

E

A

G

E

A

R

V

L

R

K

S

V

F

F

E

E

K

R

V

V

Y

Y

P

P

F

F

G

G

W

W

L

L

G

G

L

L

A

A

D

D

V

T

A

P

P

P

V

V

S

S

N

H

E

E

L

L

I

I

Y
|
Y

A

A

N

N

H

H

P

P

R

R

G

G

F

F

A

A

L

L

C

C

S

S

M

Q

R

R

S

S

A

A

T

T

R

R

S

S

R

R

Y

Y

I

V

Q

Q

C

V

P

P

L

L

D

S

A

E

K

K

I

V

E

E

D

D

W

W

S

S

D

D

D

E

R

R

F

F

W

W

D

T

E

E

L

L

R

K

R

A

R

R

L

L

P

P

A

S

H

E

H

V

A

A

E

E

A

K

L

L

T

V

T

T

A

G

P

P

S

S

F

L

E

E

K

K

S

S

I

I

A

A

P

P

L

L

R

R

S

S

F

F

V

V

T

V

E

E

P

P

M

M

R

Q

F

H

G

G

R

R

L

L

F

F

L

L

V

A

G

G

D
|
D

A

A

H

H

I

I

V

V

P

P

P
|
P

T

T

G

G

A
|
A

K
|
K

G
|
G

L
|
L

N
|
N

L

L

A

A

S

S

D

D

V

V

H

S

Y

T

L

L

F

Y

G

R

G

L

L

L

E

K

H

A

Y

Y

R

R

E

E

G

G

S

R

D

G

S

E

G

L

I

L

D

E

A

R

Y

Y

S

S

Q

A

T

I

A

C

L

L

T

R

R

R

V

I

W

W

K

K

A

A

V

E

R

R

F

F

S

S

W

W

M

M

T

T

T

S

M

V

M

L

H

H

R

R

F

F

P

P

D

D

T

T

S

D

D

A

F

F

D

S

Q

Q

K

R

I

I

Q

Q

E

Q

A

T

E

E

L

L

D

E

Y

Y

L

Y

T

L

H

G

S

S

R

E

A

A

A

G

S

L

M

A

V

T

L

I

A

A

E

E

N

N

Y
|
Y

V

V

G

G

L

L

P

P

F

Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding 8-demethyl-8-dimethylamino-flavin-adenine-dinucleotide: G9 (= G9), G11 (= G11), P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), R44 (= R44), A45 (= A45), G46 (= G46), V47 (= V47), Q102 (= Q101), D159 (= D158), I164 (≠ T163), D286 (= D285), A296 (= A295), K297 (= K296), G298 (= G297), L299 (= L298), N300 (= N299)

P20586 p-hydroxybenzoate hydroxylase; PHBH; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 7 papers)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/394 of P20586

query
sites
P20586

M

M

R

K

T

T

Q

Q

V

V

A

A

I

I

G

G

S

A

G

G

P

P

S
|
S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R

R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R
|
R

V
x
I

R
|
R

A
|
A

G
|
G

V
|
V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

L

M

H

A

A

R

E

D

G

G

L

L

P

V

H

H

D

E

G

G

F

V

S

E

L

I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
|
Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

R

A

R

R

E

E

A

A

C

C

G

G

S

A

L

T

S

T

I

V

Y

Y

D

Q

A

A

D

E

V

V

A

R

P

L

H

H

D

D

V

L

D

Q

G

G

G

E

S

R

P

P

F

Y

V

V

T

T

Y

F

T

E

K

R

D

D

G

G

I

E

G

R

H

L

R

R

I

L

D

D

C

C

D

D

F

Y

I

I

A

A

G

G

C

C

D

D

G

G

F

F

H

H

G

G

T

I

S

S

R

R

R

Q

A

S

L

I

P

P

E

A

G

E

A

R

V

L

R

K

S

V

F

F

E

E

K

R

V

V

Y

Y

P

P

F

F

G

G

W

W

L

L

G

G

L

L

A

A

D

D

V

T

A

P

P

P

V

V

S

S

N

H

E

E

L

L

I

I

Y
|
Y

A

A

N

N

H

H

P

P

R

R

G

G

F

F

A

A

L

L

C

C

S
|
S

M
x
Q

R
|
R

S

S

A

A

T

T

R

R

S

S

R
|
R

Y

Y

Y
|
Y

I

V

Q

Q

C

V

P

P

L

L

D

S

A

E

K

K

I

V

E

E

D

D

W

W

S

S

D

D

D

E

R

R

F

F

W

W

D

T

E

E

L

L

R

K

R

A

R

R

L

L

P

P

A

S

H

E

H

V

A

A

E

E

A

K

L

L

T

V

T

T

A

G

P

P

S

S

F

L

E

E

K

K

S

S

I

I

A

A

P

P

L

L

R

R

S

S

F

F

V

V

T

V

E

E

P

P

M

M

R

Q

F

H

G

G

R

R

L

L

F

F

L

L

V

A

G

G

D
|
D

A

A

H

H

I

I

V

V

P

P

P
|
P

T

T

G

G

A

A

K

K

G

G

L
|
L

N
|
N

L

L

A

A

S

S

D

D

V

V

H

S

Y

T

L

L

F

Y

G

R

G

L

L

L

E

K

H

A

Y

Y

R

R

E

E

G

G

S

R

D

G

S

E

G

L

I

L

D

E

A

R

Y

Y

S

S

Q

A

T

I

A

C

L

L

T

R

R

R

V

I

W

W

K

K

A

A

V

E

R

R

F

F

S

S

W

W

M

M

T

T

T

S

M

V

M

L

H

H

R

R

F

F

P

P

D

D

T

T

S

D

D

A

F

F

D

S

Q

Q

K

R

I

I

Q

Q

E

Q

A

T

E

E

L

L

D

E

Y

Y

L

Y

T

L

H

G

S

S

R

E

A

A

A

G

S

L

M

A

V

T

L

I

A

A

E

E

N

N

Y
|
Y

V

V

G

G

L

L

P

P

F

Y

S13 (= S13) binding FAD
E32 (≠ D32) binding FAD
RIRAGV 42:47 (≠ RVRAGV 42:47) binding FAD
A45 (= A45) mutation to G: The positions of the substrate and the flavin are not altered.
Q102 (= Q101) binding FAD
Y201 (= Y200) Important for catalytic activity; binding substrate; mutation to F: Reduction of hydroxylase activity.
SQR 212:214 (≠ SMR 211:213) binding substrate
R220 (= R219) mutation to Q: Lower affinity for p-OHB than the wild-type.
Y222 (= Y221) binding substrate
D286 (= D285) binding FAD
P293 (= P292) binding substrate
LN 299:300 (= LN 298:299) binding FAD
N300 (= N299) mutation to D: The side chain of Asp300 moves away from the flavin, disrupting the interactions of the carboxamide group with the flavin O(2) atom, and the alpha-helix H10 that begins at residue 297 is displaced, altering its dipole interactions with the flavin ring.
Y385 (= Y384) Important for catalytic activity; mutation to F: The positions of the substrate and the flavin are not altered.

1k0lA Pseudomonas aeruginosa phbh r220q free of p-ohb (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/394 of 1k0lA

query
sites
1k0lA

M

M

R

K

T

T

Q

Q

V

V

A

A

I

I

G

G

S

A

G

G

P
|
P

S
|
S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
|
R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R
|
R

V

I

R
|
R

A
|
A

G

G

V

V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

L

M

H

A

A

R

E

D

G

G

L

L

P

V

H
|
H

D

E

G

G

F

V

S

E

L

I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
|
Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

R

A

R

R

E

E

A

A

C

C

G

G

S

A

L

T

S

T

I

V

Y

Y

D

Q

A

A

D

E

V
|
V

A

R

P

L

H

H

D
|
D

V

L

D
x
Q

G

G

G

E

S

R

P

P

F

Y

V

V

T

T

Y

F

T

E

K

R

D

D

G

G

I

E

G

R

H

L

R

R

I

L

D

D

C

C

D

D

F

Y

I

I

A

A

G

G

C

C

D
|
D

G
|
G

F

F

H

H

G

G

T

I

S

S

R

R

R

Q

A

S

L

I

P

P

E

A

G

E

A

R

V

L

R

K

S

V

F

F

E

E

K

R

V

V

Y

Y

P

P

F

F

G

G

W

W

L

L

G

G

L

L

A

A

D

D

V

T

A

P

P

P

V

V

S

S

N

H

E

E

L

L

I

I

Y
|
Y

A

A

N

N

H

H

P

P

R

R

G

G

F

F

A

A

L

L

C

C

S

S

M

Q

R

R

S

S

A

A

T

T

R

R

S

S

R

Q

Y

Y

I

V

Q

Q

C

V

P

P

L

L

D

S

A

E

K

K

I

V

E

E

D

D

W

W

S

S

D

D

D

E

R

R

F

F

W

W

D

T

E

E

L

L

R

K

R

A

R

R

L

L

P

P

A

S

H

E

H

V

A

A

E

E

A

K

L

L

T

V

T

T

A

G

P

P

S

S

F

L

E

E

K

K

S

S

I

I

A

A

P

P

L

L

R

R

S

S

F

F

V

V

T

V

E

E

P

P

M

M

R

Q

F

H

G

G

R

R

L

L

F

F

L

L

V

A

G

G

D
|
D

A

A

H

H

I

I

V

V

P

P

P
|
P

T

T

G

G

A
|
A

K
|
K

G
|
G

L
|
L

N

N

L

L

A

A

S

S

D

D

V

V

H

S

Y

T

L

L

F

Y

G

R

G

L

L

L

E

K

H

A

Y

Y

R

R

E

E

G

G

S

R

D

G

S

E

G

L

I

L

D

E

A

R

Y

Y

S

S

Q

A

T

I

A

C

L

L

T

R

R

R

V

I

W

W

K

K

A

A

V

E

R

R

F

F

S

S

W

W

M

M

T

T

T

S

M

V

M

L

H

H

R

R

F

F

P

P

D

D

T

T

S

D

D

A

F

F

D

S

Q

Q

K

R

I

I

Q

Q

E

Q

A

T

E

E

L

L

D

E

Y

Y

L

Y

T

L

H

G

S

S

R

E

A

A

A

G

S

L

M

A

V

T

L

I

A

A

E

E

N

N

Y
|
Y

V

V

G

G

L

L

P

P

F

Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding flavin-adenine dinucleotide: P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), R44 (= R44), A45 (= A45), Q102 (= Q101), V127 (= V126), D159 (= D158), G160 (= G159), D286 (= D285), A296 (= A295), G298 (= G297), L299 (= L298)
binding sulfite ion: D131 (= D130), Q133 (≠ D132)

1k0jA Pseudomonas aeruginosa phbh r220q in complex with NADPH and free of p- ohb (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/394 of 1k0jA

query
sites
1k0jA

M

M

R

K

T

T

Q

Q

V

V

A

A

I

I

G

G

S

A

G

G

P
|
P

S
|
S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
|
R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R
|
R

V

I

R
|
R

A

A

G
|
G

V
|
V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

L

M

H

A

A

R

E

D

G

G

L

L

P

V

H
|
H

D

E

G

G

F

V

S

E

L

I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
|
Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

R

A

R

R

E

E

A

A

C

C

G

G

S

A

L

T

S

T

I

V

Y

Y

D

Q

A

A

D

E

V

V

A

R

P

L

H

H

D

D

V

L

D

Q

G

G

G

E

S

R

P

P

F

Y

V

V

T

T

Y

F

T

E

K

R

D

D

G

G

I

E

G

R

H

L

R

R

I

L

D

D

C

C

D

D

F

Y

I

I

A

A

G

G

C

C

D
|
D

G

G

F
|
F

H
|
H

G

G

T

I

S

S

R

R

R

Q

A

S

L

I

P

P

E

A

G

E

A

R

V

L

R

K

S

V

F

F

E

E

K

R

V

V

Y

Y

P

P

F

F

G

G

W

W

L

L

G

G

L

L

A

A

D

D

V

T

A

P

P

P

V

V

S

S

N

H

E

E

L

L

I

I

Y
|
Y

A

A

N

N

H

H

P

P

R

R

G

G

F

F

A

A

L

L

C

C

S

S

M

Q

R

R

S

S

A

A

T

T

R

R

S

S

R

Q

Y

Y

I

V

Q

Q

C

V

P

P

L

L

D

S

A

E

K

K

I

V

E

E

D

D

W

W

S

S

D

D

D

E

R

R

F

F

W

W

D

T

E

E

L

L

R

K

R

A

R

R

L

L

P

P

A

S

H

E

H

V

A

A

E

E

A

K

L

L

T

V

T

T

A

G

P

P

S

S

F

L

E

E

K

K

S

S

I

I

A

A

P

P

L

L

R
|
R

S

S

F

F

V

V

T

V

E

E

P

P

M

M

R

Q

F

H

G

G

R

R

L

L

F

F

L

L

V

A

G

G

D
|
D

A

A

H

H

I

I

V

V

P

P

P
|
P

T

T

G

G

A

A

K
|
K

G
|
G

L
|
L

N
|
N

L

L

A

A

S

S

D

D

V

V

H

S

Y

T

L

L

F

Y

G

R

G

L

L

L

E

K

H

A

Y

Y

R

R

E

E

G

G

S

R

D

G

S

E

G

L

I

L

D

E

A

R

Y

Y

S

S

Q

A

T

I

A

C

L

L

T

R

R

R

V

I

W

W

K

K

A

A

V

E

R

R

F

F

S

S

W

W

M

M

T

T

T

S

M

V

M

L

H

H

R

R

F

F

P

P

D

D

T

T

S

D

D

A

F

F

D

S

Q

Q

K

R

I

I

Q

Q

E

Q

A

T

E

E

L

L

D

E

Y

Y

L

Y

T

L

H

G

S

S

R

E

A

A

A

G

S

L

M

A

V

T

L

I

A

A

E

E

N

N

Y
|
Y

V

V

G

G

L

L

P

P

F

Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding flavin-adenine dinucleotide: P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), G46 (= G46), V47 (= V47), Q102 (= Q101), D159 (= D158), D286 (= D285), P293 (= P292), G298 (= G297), L299 (= L298), N300 (= N299)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R44 (= R44), F161 (= F160), H162 (= H161), R269 (= R268)

1pbcA Crystal structures of wild-type p-hydroxybenzoate hydroxylase complexed with 4-aminobenzoate, 2,4-dihydroxybenzoate and 2-hydroxy- 4-aminobenzoate and of the try222ala mutant, complexed with 2- hydroxy-4-aminobenzoate. Evidence for a proton channel and a new binding mode of the flavin ring (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/391 of 1pbcA

query
sites
1pbcA

M

M

R

K

T

T

Q

Q

V

V

A

A

I

I

G
|
G

S

A

G

G

P
|
P

S
|
S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
|
R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R
|
R

V

I

R
|
R

A
|
A

G

G

V
|
V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

L

M

H

A

A

R

E

D

G

G

L

L

P

V

H
|
H

D

E

G

G

F

V

S

E

L

I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
|
Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

R

A

R

R

E

E

A

A

C

S

G

G

S

A

L

T

S

T

I

V

Y

Y

D

Q

A

A

D

E

V

V

A

R

P

L

H

H

D

D

V

L

D

Q

G

G

G

E

S

R

P

P

F

Y

V

V

T

T

Y

F

T

E

K

R

D

D

G

G

I

E

G

R

H

L

R

R

I

L

D

D

C

C

D

D

F

Y

I

I

A

A

G

G

C

C

D
|
D

G

G

F

F

H

H

G

G

T
x
I

S

S

R

R

R

Q

A

S

L

I

P

P

E

A

G

E

A

R

V

L

R

K

S

V

F

F

E

E

K

R

V

V

Y

Y

P

P

F

F

G

G

W
|
W

L

L

G

G

L

L

A

A

D

D

V

T

A

P

P

P

V

V

S

S

N

H

E

E

L
|
L

I

I

Y
|
Y

A

A

N

N

H

H

P

P

R

R

G

G

F

F

A

A

L
|
L

C

C

S
|
S

M

Q

R
|
R

S

S

A

A

T

T

R

R

S

S

R

R

Y

Y

Y
|
Y

I

V

Q

Q

C

V

P

P

L

L

D

T

A

E

K

K

I

V

E

E

D

D

W

W

S

S

D

D

D

E

R

R

F

F

W

W

D

T

E

E

L

L

R

K

R

A

R

R

L

L

P

P

A

A

H

E

H

V

A

A

E

E

A

K

L

L

T

V

T

T

A

G

P

P

S

S

F

L

E

E

K

K

S

S

I

I

A

A

P

P

L

L

R

R

S

S

F

F

V

V

T

V

E

E

P

P

M

M

R

Q

F

H

G

G

R

R

L

L

F

F

L

L

V

A

G
|
G

D
|
D

A

A

H

H

I

I

V

V

P

P

P
|
P

T
|
T

G

G

A

A

K
|
K

G
|
G

L

L

N

N

L

L

A

A

S

S

D

D

V

V

H

S

Y

T

L

L

F

Y

G

R

G

L

L

L

E

K

H

A

Y

Y

R

R

E

E

G

G

S

R

D

G

S

E

G

L

I

L

D

E

A

R

Y

Y

S

S

Q

A

T

I

A

C

L

L

T

R

R

R

V

I

W

W

K

K

A

A

V

E

R

R

F

F

S

S

W

W

M

M

T

T

T

S

M

V

M

L

H

H

R

R

F

F

P

P

D

D

T

T

S

D

D

A

F

F

D

S

Q

Q

K

R

I

I

Q

Q

E

Q

A

T

E

E

L

L

D

E

Y

Y

L

Y

T

L

H

G

S

S

R

E

A

A

A

G

S

L

M

A

V

T

L

I

A

A

E

E

N

N

Y
|
Y

V

V

G

G

L

L

P

P

F

Y

active site: H72 (= H72), Y201 (= Y200), P293 (= P292), K297 (= K296), Y385 (= Y384)
binding 2-hydroxy-4-aminobenzoic acid: V47 (= V47), W185 (= W184), L199 (= L198), Y201 (= Y200), L210 (= L209), S212 (= S211), R214 (= R213), Y222 (= Y221), P293 (= P292), T294 (= T293)
binding flavin-adenine dinucleotide: G9 (= G9), P12 (= P12), S13 (= S13), E32 (≠ D32), R33 (= R33), R42 (= R42), R44 (= R44), A45 (= A45), Q102 (= Q101), D159 (= D158), I164 (≠ T163), G285 (= G284), D286 (= D285), G298 (= G297)

1bf3A P-hydroxybenzoate hydroxylase (phbh) mutant with cys 116 replaced by ser (c116s) and arg 42 replaced by lys (r42k), in complex with fad and 4-hydroxybenzoic acid (see paper)
65% identity, 100% coverage: 1:389/389 of query aligns to 1:390/391 of 1bf3A

query
sites
1bf3A

M

M

R

K

T

T

Q

Q

V

V

A

A

I

I

G

G

S

A

G

G

P
|
P

S
|
S

G

G

L

L

G

G

Q

Q

L

L

T

H

E

K

A

A

G

G

V

I

D

D

N

N

V

V

I

I

L

L

D
x
E

R
|
R

V

Q

S

T

K

P

N

D

Y

Y

I

V

L

L

G

G

R

K

V

I

R
|
R

A
|
A

G
|
G

V
|
V

L

L

E

E

E

Q

G

G

T

M

V

V

G

D

L

L

D

R

E

E

A

A

K

G

A

V

G

D

A

R

R

R

L

M

H

A

A

R

E

D

G

G

L

L

P

V

H
|
H

D

E

G

G

F

V

S

E

L

I

A

A

F

F

D

A

G

G

R

Q

D

R

H

R

R

R

I

I

D

D

L

L

H

K

E

R

L

L

T

S

G

G

-

G

R

K

R

T

V

V

T

T

V

V

Y

Y

G

G

Q
|
Q

T

T

E

E

V

V

T

T

R

R

D

D

L

L

M

M

Q

E

R

A

R

R

E

E

A

A

C

S

G

G

S

A

L

T

S

T

I

V

Y

Y

D

Q

A

A

D

E

V

V

A

R

P

L

H

H

D

D

V

L

D

Q

G

G

G

E

S

R

P

P

F

Y

V

V

T

T

Y

F

T

E

K

R

D

D

G

G

I

E

G

R

H

L

R

R

I

L

D

D

C

C

D

D

F

Y

I

I

A

A

G

G

C

C

D
|
D

G

G

F

F

H

H

G

G

T

I

S

S

R

R

R

Q

A

S

L

I

P

P

E

A

G

E

A

R

V

L

R

K

S

V

F

F

E

E

K

R

V

V

Y

Y

P

P

F

F

G

G

W

W

L

L

G

G

L

L

A

A

D

D

V

T

A

P

P

P

V

V

S

S

N

H

E

E

L

L

I

I

Y
|
Y

A

A

N

N

H

H

P

P

R

R

G

G

F

F

A

A

L

L

C

C

S

S

M

Q

R

R

S

S

A

A

T

T

R

R

S

S

R

R

Y

Y

I

V

Q

Q

C

V

P

P

L

L

D

T

A

E

K

K

I

V

E

E

D

D

W

W

S

S

D

D

D

E

R

R

F

F

W

W

D

T

E

E

L

L

R

K

R

A

R

R

L

L

P

P

A

A

H

E

H

V

A

A

E

E

A

K

L

L

T

V

T

T

A

G

P

P

S

S

F

L

E

E

K

K

S

S

I

I

A

A

P

P

L

L

R

R

S

S

F

F

V

V

T

V

E

E

P

P

M

M

R

Q

F

H

G

G

R

R

L

L

F

F

L

L

V

A

G

G

D
|
D

A

A

H

H

I

I

V

V

P

P

P
|
P

T

T

G

G

A
|
A

K
|
K

G
|
G

L
|
L

N
|
N

L

L

A

A

S

S

D

D

V

V

H

S

Y

T

L

L

F

Y

G

R

G

L

L

L