SitesBLAST
Comparing WP_042119745.1 NCBI__GCF_000092045.1:WP_042119745.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
38% identity, 97% coverage: 9:260/260 of query aligns to 14:266/266 of O53561
- K135 (≠ T126) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 126:133, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ R133) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
36% identity, 95% coverage: 13:258/260 of query aligns to 21:266/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
32% identity, 99% coverage: 3:260/260 of query aligns to 4:259/259 of 5zaiC
- active site: A65 (≠ W64), F70 (≠ A69), S82 (≠ G80), R86 (≠ F84), G110 (= G108), E113 (= E111), P132 (= P130), E133 (= E131), I138 (≠ T136), P140 (≠ A138), G141 (≠ D139), A226 (≠ K224), F236 (≠ Y237)
- binding coenzyme a: K24 (= K22), L25 (≠ A23), A63 (= A62), G64 (= G63), A65 (≠ W64), D66 (= D65), I67 (≠ L66), P132 (= P130), R166 (≠ W163), F248 (= F249), K251 (= K252)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
33% identity, 98% coverage: 3:258/260 of query aligns to 4:256/258 of 1mj3A
- active site: A68 (≠ W64), M73 (≠ A69), S83 (≠ Y79), L85 (≠ V81), G109 (= G108), E112 (= E111), P131 (= P130), E132 (= E131), T137 (= T136), P139 (≠ A138), G140 (≠ D139), K225 (= K224), F235 (≠ Y237)
- binding hexanoyl-coenzyme a: K26 (= K22), A27 (= A23), L28 (vs. gap), A30 (= A25), A66 (= A62), G67 (= G63), A68 (≠ W64), D69 (= D65), I70 (≠ L66), G109 (= G108), P131 (= P130), E132 (= E131), L135 (≠ S134), G140 (≠ D139)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
32% identity, 98% coverage: 3:258/260 of query aligns to 2:256/258 of 1ey3A
- active site: A66 (≠ W64), M71 (≠ A69), S81 (≠ D76), L85 (≠ F84), G109 (= G108), E112 (= E111), P131 (= P130), E132 (= E131), T137 (= T136), P139 (≠ A138), G140 (≠ D139), K225 (= K224), F235 (≠ Y237)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K22), L26 (vs. gap), A28 (= A25), A64 (= A62), G65 (= G63), A66 (≠ W64), D67 (= D65), I68 (≠ L66), L85 (≠ F84), W88 (≠ L87), G109 (= G108), P131 (= P130), L135 (≠ S134), G140 (≠ D139)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
32% identity, 98% coverage: 3:258/260 of query aligns to 4:258/260 of 1dubA
- active site: A68 (≠ W64), M73 (≠ A69), S83 (≠ D76), L87 (≠ F84), G111 (= G108), E114 (= E111), P133 (= P130), E134 (= E131), T139 (= T136), P141 (≠ A138), G142 (≠ D139), K227 (= K224), F237 (≠ Y237)
- binding acetoacetyl-coenzyme a: K26 (= K22), A27 (= A23), L28 (vs. gap), A30 (= A25), A66 (= A62), A68 (≠ W64), D69 (= D65), I70 (≠ L66), Y107 (≠ L104), G110 (= G107), G111 (= G108), E114 (= E111), P133 (= P130), E134 (= E131), L137 (≠ S134), G142 (≠ D139), F233 (= F230), F249 (= F249)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
32% identity, 98% coverage: 3:258/260 of query aligns to 34:288/290 of P14604
- E144 (= E111) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E131) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
32% identity, 98% coverage: 3:258/260 of query aligns to 3:252/254 of 2dubA
- active site: A67 (≠ W64), M72 (≠ A69), S82 (≠ G85), G105 (= G108), E108 (= E111), P127 (= P130), E128 (= E131), T133 (= T136), P135 (≠ A138), G136 (≠ D139), K221 (= K224), F231 (≠ Y237)
- binding octanoyl-coenzyme a: K25 (= K22), A26 (= A23), L27 (vs. gap), A29 (= A25), A65 (= A62), A67 (≠ W64), D68 (= D65), I69 (≠ L66), K70 (= K67), G105 (= G108), E108 (= E111), P127 (= P130), E128 (= E131), G136 (≠ D139), A137 (= A140)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
35% identity, 98% coverage: 5:260/260 of query aligns to 6:257/257 of 6slbAAA
- active site: Q64 (≠ W64), F69 (≠ S71), L80 (≠ V81), N84 (≠ G85), A108 (≠ G108), S111 (≠ E111), A130 (≠ P130), F131 (≠ E131), L136 (≠ T136), P138 (≠ A138), D139 (= D139), A224 (≠ K227), G234 (≠ Y237)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K58), A62 (= A62), Q64 (≠ W64), D65 (= D65), L66 (= L66), Y76 (≠ G77), A108 (≠ G108), F131 (≠ E131), D139 (= D139)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
33% identity, 96% coverage: 9:258/260 of query aligns to 13:258/260 of 2hw5C
- active site: A68 (≠ W64), M73 (≠ A69), S83 (≠ G80), L87 (≠ F84), G111 (= G108), E114 (= E111), P133 (= P130), E134 (= E131), T139 (= T136), P141 (≠ A138), G142 (≠ D139), K227 (= K224), F237 (≠ Y237)
- binding crotonyl coenzyme a: K26 (= K22), A27 (= A23), L28 (vs. gap), A30 (= A25), K62 (= K58), I70 (≠ L66), F109 (≠ C106)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
33% identity, 99% coverage: 3:260/260 of query aligns to 3:256/256 of 3h81A
- active site: A64 (≠ W64), M69 (≠ A69), T79 (≠ D76), F83 (= F84), G107 (= G108), E110 (= E111), P129 (= P130), E130 (= E131), V135 (≠ T136), P137 (≠ A138), G138 (≠ D139), L223 (vs. gap), F233 (≠ Y237)
- binding calcium ion: F233 (≠ Y237), Q238 (= Q242)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
33% identity, 98% coverage: 3:257/260 of query aligns to 4:254/255 of 3q0jC
- active site: A65 (≠ W64), M70 (≠ A69), T80 (≠ D76), F84 (= F84), G108 (= G108), E111 (= E111), P130 (= P130), E131 (= E131), V136 (≠ T136), P138 (≠ A138), G139 (≠ D139), L224 (vs. gap), F234 (≠ Y237)
- binding acetoacetyl-coenzyme a: Q23 (≠ K22), A24 (= A23), L25 (vs. gap), A27 (= A25), A63 (= A62), G64 (= G63), A65 (≠ W64), D66 (= D65), I67 (≠ L66), K68 (= K67), M70 (≠ A69), F84 (= F84), G107 (= G107), G108 (= G108), E111 (= E111), P130 (= P130), E131 (= E131), P138 (≠ A138), G139 (≠ D139), M140 (vs. gap)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 98% coverage: 3:257/260 of query aligns to 4:254/255 of 3q0gC
- active site: A65 (≠ W64), M70 (≠ A69), T80 (≠ D76), F84 (= F84), G108 (= G108), E111 (= E111), P130 (= P130), E131 (= E131), V136 (≠ T136), P138 (≠ A138), G139 (≠ D139), L224 (vs. gap), F234 (≠ Y237)
- binding coenzyme a: L25 (vs. gap), A63 (= A62), I67 (≠ L66), K68 (= K67), Y104 (≠ L104), P130 (= P130), E131 (= E131), L134 (≠ S134)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
34% identity, 98% coverage: 5:260/260 of query aligns to 3:245/245 of 6slaAAA
- active site: Q61 (≠ W64), L68 (≠ A74), N72 (≠ D78), A96 (≠ G108), S99 (≠ E111), A118 (≠ P130), F119 (≠ E131), L124 (≠ T136), P126 (≠ A138), N127 (≠ D139), A212 (≠ K227), G222 (≠ Y237)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ A23), A59 (= A62), Q61 (≠ W64), D62 (= D65), L63 (= L66), L68 (≠ A74), Y71 (≠ G77), A94 (≠ C106), G95 (= G107), A96 (≠ G108), F119 (≠ E131), I122 (≠ S134), L124 (≠ T136), N127 (≠ D139), F234 (= F249), K237 (= K252)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 98% coverage: 3:257/260 of query aligns to 3:249/250 of 3q0gD
- active site: A64 (≠ W64), M69 (≠ A69), T75 (≠ D76), F79 (= F84), G103 (= G108), E106 (= E111), P125 (= P130), E126 (= E131), V131 (≠ T136), P133 (≠ A138), G134 (≠ D139), L219 (vs. gap), F229 (≠ Y237)
- binding Butyryl Coenzyme A: F225 (= F230), F241 (= F249)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
33% identity, 91% coverage: 24:260/260 of query aligns to 27:261/261 of 5jbxB