SitesBLAST
Comparing WP_042119916.1 NCBI__GCF_000092045.1:WP_042119916.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
A0A0K2JL82 Nitrosuccinate lyase; EC 4.3.99.5 from Streptomyces cremeus (see paper)
33% identity, 90% coverage: 30:343/348 of query aligns to 42:375/476 of A0A0K2JL82
- N93 (≠ V81) mutation to A: Slight decrease in activity.
- D125 (= D110) mutation D->N,V: Almost loss of activity.
- R137 (≠ A122) binding fumarate
- R140 (≠ F125) binding fumarate
- R201 (vs. gap) binding fumarate
- H253 (≠ Q225) mutation to A: Loss of activity.
- S302 (= S270) mutation to A: Loss of activity.
- K308 (= K276) binding fumarate; mutation to A: Loss of activity.
- N310 (= N278) binding fumarate; mutation to A: Loss of activity.
- R341 (= R309) mutation to A: Loss of activity.
5xnzA Crystal structure of cred complex with fumarate (see paper)
31% identity, 90% coverage: 30:343/348 of query aligns to 28:344/439 of 5xnzA
P12047 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor; EC 4.3.2.2 from Bacillus subtilis (strain 168) (see paper)
24% identity, 93% coverage: 19:342/348 of query aligns to 9:334/431 of P12047
- H89 (= H103) mutation to Q: Abolishes enzyme activity.
- H141 (≠ R155) mutation to Q: Abolishes enzyme activity.
- Q212 (≠ R222) mutation to E: Decreases catalytic activity 1000-fold.; mutation to M: Abolishes enzyme activity.
- N270 (= N278) mutation N->D,L: Abolishes enzyme activity.
- R301 (≠ Q307) mutation R->K,Q: Abolishes enzyme activity.
2x75A Staphylococcus aureus adenylosuccinate lyase (see paper)
23% identity, 91% coverage: 18:332/348 of query aligns to 7:323/427 of 2x75A
Sites not aligning to the query:
5nx9D Crystal structure of neanderthal adenylosuccinate lyase (adsl) in complex with its products amp and fumarate (see paper)
28% identity, 54% coverage: 102:290/348 of query aligns to 99:302/477 of 5nx9D
- active site: T151 (= T154), H152 (≠ R155), S283 (= S271), K288 (= K276), E295 (= E283)
- binding 2-[9-(3,4-dihydroxy-5-phosphonooxymethyl-tetrahydro-furan-2-yl)-9h-purin-6-ylamino]-succinic acid: T151 (= T154), H152 (≠ R155)
- binding adenosine monophosphate: S105 (= S108), Q234 (= Q223), R296 (≠ V284)
- binding fumaric acid: S105 (= S108), Q234 (= Q223), S282 (= S270), S283 (= S271), K288 (= K276)
Sites not aligning to the query:
P30566 Adenylosuccinate lyase; ADSL; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Homo sapiens (Human) (see 13 papers)
28% identity, 54% coverage: 102:290/348 of query aligns to 106:309/484 of P30566
- Y114 (≠ D110) to H: in ADSLD; severe; total loss of activity; dbSNP:rs374259530
- R141 (≠ A137) to W: in ADSLD; severe; dbSNP:rs756210458
- H159 (≠ R155) active site, Proton donor/acceptor
- R190 (≠ S186) to Q: in ADSLD; moderate; dbSNP:rs28941471
- R194 (≠ Q190) to C: in ADSLD; severe; reduces protein stability; dbSNP:rs1465152683
- K246 (≠ R228) to E: in ADSLD; moderate; strongly reduced catalytic activity; dbSNP:rs119450944
- D268 (= D251) to N: in ADSLD; severe; total loss of activity; dbSNP:rs746501563
- S289 (= S270) active site, Proton donor/acceptor
- R303 (≠ V284) to C: in ADSLD; mild; strongly reduced activity with SAMP, but only slightly reduced activity with SAICAR; abolishes cooperativity; dbSNP:rs373458753
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine; A → V: in ADSLD; severe; dbSNP:rs143083947
- 3 A → V: in ADSLD; severe
- 26 M → L: in ADSLD; severe; dbSNP:rs1311171245
- 72 I → V: in ADSLD; severe
- 100 P → A: in ADSLD; moderate; dbSNP:rs119450942
- 311 L → V: in ADSLD; severe; slightly reduced enzyme activity; dbSNP:rs2044791112
- 318 P → L: in ADSLD; severe; dbSNP:rs202064195
- 364 V → M: in ADSLD; severe; dbSNP:rs370851726
- 374 R → W: in ADSLD; severe; dbSNP:rs376533026
- 395 S → R: in ADSLD; severe
- 396 R → C: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs755492501; R → H: in ADSLD; severe; abolishes cooperativity and reduces enzyme activity; dbSNP:rs763542069
- 422 D → Y: in ADSLD; moderate; dbSNP:rs119450943
- 423 L → V: in ADSLD; moderate
- 426 R → H: in ADSLD; severe; most frequent mutation; dbSNP:rs119450941
- 430 D → N: in ADSLD; mild; dbSNP:rs554254383
- 438 S → P: in ADSLD; severe; dbSNP:rs119450940
- 447 S → P: in ADSLD; severe; dbSNP:rs777821034
- 450 T → S: in ADSLD; moderate; dbSNP:rs372895468
- 452 R → P: in ADSLD; severe; dbSNP:rs775671027
Q9X0I0 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
23% identity, 92% coverage: 24:343/348 of query aligns to 14:335/431 of Q9X0I0
- H141 (≠ R155) active site, Proton donor/acceptor
5nxaB Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
28% identity, 65% coverage: 66:290/348 of query aligns to 2:240/415 of 5nxaB
- active site: T89 (= T154), H90 (≠ R155), S221 (= S271), K226 (= K276), E233 (= E283)
- binding aminoimidazole 4-carboxamide ribonucleotide: R13 (≠ G80), Y14 (≠ V81), M230 (≠ V280), R234 (≠ V284)
- binding fumaric acid: S220 (= S270), S221 (= S271), M223 (= M273), K226 (= K276), N228 (= N278)
- binding n-{[5-amino-1-(5-o-phosphono-beta-d-arabinofuranosyl)-1h-imidazol-4-yl]carbonyl}-l-aspartic acid: S43 (= S108), T89 (= T154), H90 (≠ R155), Q172 (= Q223)
Sites not aligning to the query:
4efcA Crystal structure of adenylosuccinate lyase from trypanosoma brucei, tb427tmp.160.5560
25% identity, 73% coverage: 31:283/348 of query aligns to 39:312/452 of 4efcA
- active site: H96 (≠ K78), T173 (= T154), H174 (≠ R155), S300 (= S271), K305 (= K276), E312 (= E283)
- binding adenosine monophosphate: N95 (≠ A77), H96 (≠ K78), D97 (= D79), S127 (= S108), Q128 (= Q109), I309 (≠ V280)
- binding magnesium ion: D274 (≠ K247), D278 (= D251)
Sites not aligning to the query:
5vkwB Crystal structure of adenylosuccinate lyase ade13 from candida albicans
27% identity, 54% coverage: 102:290/348 of query aligns to 103:299/469 of 5vkwB
Sites not aligning to the query:
Q05911 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 54% coverage: 102:290/348 of query aligns to 103:306/482 of Q05911
- K196 (≠ A195) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
5nxaA Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
25% identity, 54% coverage: 102:290/348 of query aligns to 99:289/464 of 5nxaA
Sites not aligning to the query:
5nxaC Crystal structure of neanderthal adenylosuccinate lyase (adsl)in complex with its products aicar and fumarate (see paper)
25% identity, 54% coverage: 102:290/348 of query aligns to 98:290/418 of 5nxaC
Sites not aligning to the query:
5nx9C Crystal structure of neanderthal adenylosuccinate lyase (adsl) in complex with its products amp and fumarate (see paper)
24% identity, 54% coverage: 102:290/348 of query aligns to 98:287/441 of 5nx9C
Sites not aligning to the query:
Query Sequence
>WP_042119916.1 NCBI__GCF_000092045.1:WP_042119916.1
MTASPFDHPFLSGLLGDDEIAPHFSAEADIRAMLSFEAALARAEAAHGLIPAEAARKIAE
ACTGFSPDLASLRSAMAKDGVVVPDFVKQLRAKVGEEAGKNLHLGATSQDVIDTSLMIRL
KAVVFLFAGRLSAIIAALEGLDRQFGGNRLMGHTRMQAAIAISVSDRLNVWRAPLATYRD
RLTEQSFPVQFGGAAGTLDKLGPQGPAVRASLAQELGLSDTRQWQSARLPIADIAGLFAS
ISGSLGKIGQDIALLAEMGGEIEVSGGGASSAMAHKQNPVAAEVLVSLARFNATALSGIH
QSLIHEQERSGAAWTLEWLLLPQMTMGTAASLRLARELTGNIKRLGTG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory