SitesBLAST
Comparing WP_042444391.1 NCBI__GCF_000010725.1:WP_042444391.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
44% identity, 94% coverage: 13:434/447 of query aligns to 10:419/430 of 6w80A
- active site: V26 (= V29), Y149 (= Y154), D241 (= D256), K269 (= K284)
- binding pyridoxal-5'-phosphate: S121 (≠ T126), G122 (= G127), T123 (= T128), Y149 (= Y154), H150 (= H155), E208 (= E223), N213 (= N228), D241 (= D256), V243 (= V258), K269 (= K284)
P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
42% identity, 97% coverage: 13:444/447 of query aligns to 3:426/426 of P23893
- K265 (= K284) mutation to R: 2% of wild-type activity.
3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
39% identity, 97% coverage: 12:444/447 of query aligns to 5:427/430 of 3bs8A
- active site: V22 (= V29), Y145 (= Y154), E207 (= E223), D240 (= D256), M243 (≠ I259), K268 (= K284), G401 (≠ N418)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G127), T119 (= T128), Y145 (= Y154), H146 (= H155), E207 (= E223), N212 (= N228), D240 (= D256), V242 (= V258), K268 (= K284)
3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
42% identity, 97% coverage: 13:444/447 of query aligns to 4:426/426 of 3fqaA
- active site: V20 (= V29), Y143 (= Y154), D238 (= D256), I241 (= I259), K266 (= K284), A400 (≠ N418)
- binding 3-aminobenzoic acid: S22 (= S31), R25 (= R34), W60 (≠ L70)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G116 (= G127), T117 (= T128), Y143 (= Y154), E205 (= E223), N210 (= N228), D238 (= D256), V240 (= V258), I241 (= I259)
2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
41% identity, 97% coverage: 13:444/447 of query aligns to 5:427/427 of 2gsaB
- active site: V21 (= V29), Y144 (= Y154), E206 (= E223), D239 (= D256), M242 (≠ I259), K267 (= K284), A401 (≠ N418)
- binding pyridoxal-5'-phosphate: G117 (= G127), T118 (= T128), Y144 (= Y154), E206 (= E223), N211 (= N228), D239 (= D256), V241 (= V258), M242 (≠ I259), K267 (= K284)
2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
41% identity, 97% coverage: 13:444/447 of query aligns to 5:427/427 of 2gsaA
- active site: V21 (= V29), Y144 (= Y154), E206 (= E223), D239 (= D256), M242 (≠ I259), K267 (= K284), A401 (≠ N418)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G127), T118 (= T128), Y144 (= Y154), H145 (= H155), G146 (= G156), N211 (= N228), D239 (= D256), V241 (= V258), K267 (= K284)
Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
38% identity, 99% coverage: 1:444/447 of query aligns to 35:472/472 of Q42522
- R92 (≠ T56) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
- G162 (= G127) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.
3usfA Crystal structure of dava-4
41% identity, 97% coverage: 13:444/447 of query aligns to 5:427/427 of 3usfA
- active site: V21 (= V29), Y144 (= Y154), D239 (= D256), M242 (≠ I259), K267 (= K284), A401 (≠ N418)
- binding (4s)-4,5-diaminopentanoic acid: S23 (= S31), V25 (≠ A33), S157 (≠ R174), K267 (= K284), E400 (= E417)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G127), T118 (= T128), Y144 (= Y154), N211 (= N228), D239 (= D256), V241 (= V258), K267 (= K284)
3fq7A Gabaculine complex of gsam (see paper)
41% identity, 97% coverage: 13:444/447 of query aligns to 5:427/427 of 3fq7A
- active site: V21 (= V29), Y144 (= Y154), D239 (= D256), M242 (≠ I259), K267 (= K284), A401 (≠ N418)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S31), V25 (≠ A33), W61 (≠ L70), G117 (= G127), T118 (= T128), Y144 (= Y154), H145 (= H155), E206 (= E223), N211 (= N228), D239 (= D256), V241 (= V258), M242 (≠ I259), K267 (= K284), G298 (= G314), T299 (= T315), E400 (= E417)
2hp2A Inter-subunit signaling in gsam (see paper)
41% identity, 97% coverage: 13:444/447 of query aligns to 5:427/427 of 2hp2A
- active site: V21 (= V29), Y144 (= Y154), D239 (= D256), M242 (≠ I259), K267 (= K284), A401 (≠ N418)
- binding (4s)-4,5-diaminopentanoic acid: G298 (= G314), T299 (= T315)
- binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S31), G117 (= G127), T118 (= T128), Y144 (= Y154), H145 (= H155), E206 (= E223), N211 (= N228), D239 (= D256), V241 (= V258), M242 (≠ I259), K267 (= K284)
- binding pyridoxal-5'-phosphate: G298 (= G314), T299 (= T315)
2hp1A Inter-subunit signaling in gsam (see paper)
41% identity, 97% coverage: 13:444/447 of query aligns to 5:427/427 of 2hp1A
- active site: V21 (= V29), Y144 (= Y154), D239 (= D256), M242 (≠ I259), K267 (= K284), A401 (≠ N418)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S31), V25 (≠ A33), W61 (≠ L70), S116 (≠ T126), G117 (= G127), T118 (= T128), Y144 (= Y154), H145 (= H155), G146 (= G156), E206 (= E223), N211 (= N228), D239 (= D256), V241 (= V258), M242 (≠ I259), K267 (= K284), E400 (= E417)
2hozA Inter-subunit signaling in gsam (see paper)
41% identity, 97% coverage: 13:444/447 of query aligns to 5:427/427 of 2hozA
- active site: V21 (= V29), Y144 (= Y154), D239 (= D256), M242 (≠ I259), K267 (= K284), A401 (≠ N418)
- binding (4s)-4,5-diaminopentanoic acid: E141 (= E151), G156 (= G173), S157 (≠ R174), P182 (= P199), N368 (= N384), E370 (≠ R386), K373 (≠ R390)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G127), T118 (= T128), Y144 (= Y154), H145 (= H155), G146 (= G156), E206 (= E223), N211 (= N228), D239 (= D256), G298 (= G314), T299 (= T315)
3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
41% identity, 93% coverage: 13:429/447 of query aligns to 6:407/423 of 3k28A
- active site: V22 (= V29), Y145 (= Y154), E202 (= E223), D235 (= D256), M238 (≠ I259), K263 (= K284), G396 (≠ N418)
- binding calcium ion: I103 (≠ T112), V106 (= V115), P107 (= P116), I109 (≠ L118)
- binding pyridoxal-5'-phosphate: G118 (= G127), T119 (= T128), Y145 (= Y154), H146 (= H155), G147 (= G156), E202 (= E223), D235 (= D256), V237 (= V258), M238 (≠ I259), K263 (= K284)
P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
39% identity, 98% coverage: 9:444/447 of query aligns to 42:474/474 of P42799
- K314 (= K284) modified: N6-(pyridoxal phosphate)lysine
5hdmB Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
39% identity, 97% coverage: 12:444/447 of query aligns to 5:428/428 of 5hdmB
- active site: V22 (= V29), Y145 (= Y154), E207 (= E223), D240 (= D256), M243 (≠ I259), K268 (= K284), A402 (≠ N418)
- binding pyridoxal-5'-phosphate: G118 (= G127), T119 (= T128), Y145 (= Y154), E207 (= E223), N212 (= N228), D240 (= D256), V242 (= V258), M243 (≠ I259), K268 (= K284)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G127), T119 (= T128), Y145 (= Y154), E207 (= E223), N212 (= N228), D240 (= D256), V242 (= V258), M243 (≠ I259), K268 (= K284)
5hdmA Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
39% identity, 97% coverage: 12:444/447 of query aligns to 5:428/428 of 5hdmA
- active site: V22 (= V29), Y145 (= Y154), E207 (= E223), D240 (= D256), M243 (≠ I259), K268 (= K284), A402 (≠ N418)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G127), T119 (= T128), Y145 (= Y154), G147 (= G156), E207 (= E223), N212 (= N228), D240 (= D256), V242 (= V258), K268 (= K284)
2e7uA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from thermus thermophilus hb8
41% identity, 96% coverage: 14:441/447 of query aligns to 6:424/424 of 2e7uA
- active site: V21 (= V29), Y144 (= Y154), E206 (= E223), D238 (= D256), M241 (≠ I259), K266 (= K284), A401 (≠ N418)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G127), T118 (= T128), Y144 (= Y154), H145 (= H155), N211 (= N228), D238 (= D256), V240 (= V258)
5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
41% identity, 96% coverage: 13:441/447 of query aligns to 3:418/420 of 5i92F
Sites not aligning to the query:
3usfB Crystal structure of dava-4
40% identity, 97% coverage: 13:444/447 of query aligns to 5:402/402 of 3usfB
- active site: V21 (= V29), Y144 (= Y154), E181 (= E223), D214 (= D256), M217 (≠ I259), K242 (= K284), A376 (≠ N418)
- binding (4s)-4,5-diaminopentanoic acid: G273 (= G314), T274 (= T315)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G127), T118 (= T128), Y144 (= Y154), E181 (= E223), N186 (= N228), D214 (= D256), V216 (= V258), M217 (≠ I259), K242 (= K284)
2hp1B Inter-subunit signaling in gsam (see paper)
39% identity, 97% coverage: 13:444/447 of query aligns to 5:398/398 of 2hp1B
- active site: V21 (= V29), Y144 (= Y154), E177 (= E223), D210 (= D256), M213 (≠ I259), K238 (= K284), A372 (≠ N418)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: G269 (= G314), T270 (= T315)
- binding (4s)-4,5-diaminopentanoic acid: S23 (= S31), V25 (≠ A33), R26 (= R34), W61 (≠ L70), Y144 (= Y154)
- binding pyridoxal-5'-phosphate: S116 (≠ T126), G117 (= G127), T118 (= T128), Y144 (= Y154), E177 (= E223), N182 (= N228), D210 (= D256), V212 (= V258), M213 (≠ I259), K238 (= K284)
Query Sequence
>WP_042444391.1 NCBI__GCF_000010725.1:WP_042444391.1
MTSSYQQPDRRRRSAALFEQARTLIPGGVNSTARATWSGWDPYPLFVERGTGSRVTDVDG
NEYIDYLLGLGPMLLGHRPPAVTKAVVDCIEQRGTVFALPSADETILARKITAAVPGLER
VRLCNTGTEAVLYTVRLARAFTGRPKLIRFEGMYHGFSDGVYWSKHPKLDAAGRDDAPTA
VPQGPGMPRGVGDSLIILPWNDVEALRTTLEREGDQIAAVLSEPIMCNTGCILPEPGYLE
AMRELTRKHGVLMILDEVITGFRIALGGAQAHYGVTPDLSVFAKGLGGGFPVAAMGGRAD
IMALVENGTVSIAGTYNANMIAVSAANATLDELAQPGMYERLYAVSDRLREGLDRLIRDL
RIPAHVVGLGPVFQLWFADRPIRNYRDAVRHADHDAFRRWWEGMLDRGILFHPGAYENLF
VSFAHSEADIDQTLAAARETLTEMMVA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory