SitesBLAST
Comparing WP_043526306.1 NCBI__GCF_000759345.1:WP_043526306.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
9br7C Succinate--hydroxymethylglutarate CoA-transferase (see paper)
42% identity, 99% coverage: 4:400/401 of query aligns to 5:397/403 of 9br7C
3ubmB Formyl-coa:oxalate coa-transferase from acetobacter aceti (see paper)
30% identity, 99% coverage: 4:400/401 of query aligns to 4:428/430 of 3ubmB
- active site: Q17 (≠ L17), E140 (≠ D146), D182 (= D175), G261 (vs. gap), G262 (vs. gap)
- binding coenzyme a: V16 (= V16), R38 (≠ H38), L72 (≠ V78), N73 (≠ D79), T74 (≠ I80), K75 (≠ A81), N96 (= N102), F97 (= F103), R98 (≠ K104), A101 (≠ G107), R104 (= R110), K125 (≠ T131), D182 (= D175), M213 (vs. gap)
O06644 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; EC 2.8.3.16 from Oxalobacter formigenes (see 4 papers)
31% identity, 100% coverage: 1:400/401 of query aligns to 1:428/428 of O06644
- Q17 (≠ L17) mutation to A: 45-fold decrease of the catalytic effiency.
- R38 (≠ H38) binding CoA
- W48 (= W48) mutation to F: Little change in the affinity binding and catalytic efficiency, and it does not display major structural changes.; mutation to P: Little change in the affinity binding and catalytic efficiency. It exhibits substrate inhibition with oxalate. It does not display major structural changes.
- R104 (= R110) binding CoA
- D169 (= D175) active site, Nucleophile; mutation to A: Loss of CoA-transferase activity.; mutation to E: Loss of CoA-transferase activity.; mutation to S: Loss of CoA-transferase activity.
- G259 (vs. gap) mutation to A: 2.5-fold decrease of the catalytic effiency.
- G260 (vs. gap) mutation to A: 25-fold decrease of the catalytic effiency. Reduction of the affinity binding for both formyl-CoA and oxalate.
1p5rA Formyl-coa transferase in complex with coenzyme a (see paper)
31% identity, 99% coverage: 4:400/401 of query aligns to 3:427/427 of 1p5rA
- active site: Q16 (≠ L17), E139 (≠ D146), D168 (= D175), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (≠ R15), V15 (= V16), Q16 (≠ L17), A17 (= A18), R37 (≠ H38), M73 (≠ I80), K74 (≠ A81), N95 (= N102), F96 (= F103), A100 (≠ G107), R103 (= R110), K136 (≠ A143), V137 (≠ G144), D168 (= D175), M199 (≠ L206)
2vjoA Formyl-coa transferase mutant variant q17a with aspartyl-coa thioester intermediates and oxalate (see paper)
31% identity, 99% coverage: 4:400/401 of query aligns to 3:427/427 of 2vjoA
- active site: A16 (≠ L17), E139 (≠ D146), D168 (= D175), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (≠ R15), A16 (≠ L17), A17 (= A18), R37 (≠ H38), L71 (≠ V78), M73 (≠ I80), N95 (= N102), F96 (= F103), G97 (≠ K104), R103 (= R110), M104 (≠ Y111), K136 (≠ A143), V137 (≠ G144), Y138 (= Y145), D168 (= D175), M199 (≠ L206)
- binding oxalate ion: G257 (vs. gap), G259 (vs. gap), Q261 (vs. gap)
2vjkA Formyl-coa transferase with aspartyl-coa thioester intermediate derived from oxalyl-coa (see paper)
31% identity, 99% coverage: 4:400/401 of query aligns to 3:427/427 of 2vjkA
- active site: Q16 (≠ L17), E139 (≠ D146), D168 (= D175), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (≠ R15), Q16 (≠ L17), A17 (= A18), R37 (≠ H38), M73 (≠ I80), K74 (≠ A81), N95 (= N102), F96 (= F103), G97 (≠ K104), R103 (= R110), M104 (≠ Y111), K136 (≠ A143), V137 (≠ G144), Y138 (= Y145), D168 (= D175), M199 (≠ L206)
- binding magnesium ion: D293 (≠ E266), D296 (≠ G269)
1t4cA Formyl-coa transferase in complex with oxalyl-coa (see paper)
31% identity, 99% coverage: 4:400/401 of query aligns to 3:427/427 of 1t4cA
- active site: Q16 (≠ L17), E139 (≠ D146), D168 (= D175), G259 (vs. gap), G260 (vs. gap)
- binding coenzyme a: H14 (≠ R15), V15 (= V16), Q16 (≠ L17), R37 (≠ H38), M73 (≠ I80), N95 (= N102), F96 (= F103), R103 (= R110), M104 (≠ Y111), V137 (≠ G144), Y138 (= Y145), D168 (= D175), M199 (≠ L206)
- binding oxalic acid: G259 (vs. gap), G260 (vs. gap)
1t3zA Formyl-coa tranferase mutant asp169 to ser (see paper)
31% identity, 99% coverage: 4:400/401 of query aligns to 3:427/427 of 1t3zA
- active site: Q16 (≠ L17), E139 (≠ D146), S168 (≠ D175), G259 (vs. gap), G260 (vs. gap)
- binding oxidized coenzyme a: H14 (≠ R15), V15 (= V16), A17 (= A18), R37 (≠ H38), K74 (≠ A81), N95 (= N102), F96 (= F103), A100 (≠ G107), R103 (= R110), M104 (≠ Y111), K136 (≠ A143), V137 (≠ G144), Y138 (= Y145), E139 (≠ D146), M199 (≠ L206)
P69902 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; Formyl-CoA transferase; EC 2.8.3.16 from Escherichia coli (strain K12) (see paper)
30% identity, 99% coverage: 1:395/401 of query aligns to 1:411/416 of P69902
1q6yA Hypothetical protein yfdw from e. Coli bound to coenzyme a (see paper)
30% identity, 99% coverage: 1:395/401 of query aligns to 1:411/417 of 1q6yA
- active site: Q17 (≠ L17), E140 (≠ D146), D169 (= D175), G248 (vs. gap), G249 (vs. gap)
- binding coenzyme a: V16 (= V16), Q17 (≠ L17), S18 (≠ A18), R38 (≠ H38), L72 (≠ V78), N73 (≠ D79), T74 (≠ I80), K75 (≠ A81), N96 (= N102), F97 (= F103), H98 (≠ K104), M105 (≠ Y111), I124 (= I130), K137 (≠ A143), A138 (≠ G144), Y139 (= Y145), D169 (= D175), M200 (≠ L206)
1pt5A Crystal structure of gene yfdw of e. Coli (see paper)
30% identity, 98% coverage: 4:395/401 of query aligns to 3:410/415 of 1pt5A