SitesBLAST
Comparing WP_043526905.1 NCBI__GCF_000759345.1:WP_043526905.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
23% identity, 85% coverage: 31:396/430 of query aligns to 23:374/383 of 5i39A
- active site: F66 (≠ S74), Q69 (= Q77), A70 (≠ I78), Q248 (≠ E271), P267 (≠ D292)
- binding flavin-adenine dinucleotide: V30 (≠ I38), G31 (= G39), G33 (= G41), I34 (≠ V42), L35 (≠ T43), V53 (≠ L61), E54 (= E62), K55 (≠ A63), Q62 (≠ A70), S63 (= S71), F66 (≠ S74), Y67 (≠ G75), Q69 (= Q77), A196 (≠ P212), A197 (= A213), G226 (≠ T240), G227 (≠ N241), W229 (≠ Y243), Q248 (≠ E271), Q250 (= Q273), G321 (= G344), M323 (≠ V346), T348 (≠ S370), G349 (= G371), W350 (≠ H372), G351 (= G373), M352 (= M374), T353 (≠ S375)
4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
24% identity, 90% coverage: 28:414/430 of query aligns to 1:394/824 of 4pabB
- active site: T53 (≠ L79), E102 (= E141), H226 (≠ N261), Y255 (= Y294)
- binding flavin-adenine dinucleotide: I11 (= I38), G12 (= G39), G14 (= G41), C15 (≠ V42), V16 (≠ T43), L35 (= L61), E36 (= E62), K37 (≠ A63), G43 (= G69), S44 (≠ A70), T45 (≠ S71), H47 (≠ R73), A48 (≠ S74), A49 (≠ G75), G50 (= G76), L51 (≠ Q77), V175 (≠ A213), A204 (≠ T240), G205 (≠ N241), W207 (≠ Y243), H226 (≠ N261), Y228 (≠ M263), G326 (= G344), I328 (≠ V346), F353 (≠ Y369), Y355 (≠ H372), G356 (= G373), I357 (≠ M374), I358 (≠ S375)
Sites not aligning to the query:
- active site: 536
- binding (6s)-5,6,7,8-tetrahydrofolate: 523, 536, 538, 550, 612, 613, 632, 639, 680, 700
Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 90% coverage: 28:414/430 of query aligns to 38:431/857 of Q63342
- CV 52:53 (≠ VT 42:43) binding FAD
- EK 73:74 (≠ EA 62:63) binding FAD
- 80:88 (vs. 69:77, 22% identical) binding FAD
- H84 (≠ R73) modified: Tele-8alpha-FAD histidine
- V212 (≠ A213) binding FAD
- W244 (≠ Y243) binding FAD
- F-GYGII 390:395 (≠ YSGHGMS 369:375) binding FAD
Sites not aligning to the query:
- 573:575 binding (6S)-5,6,7,8-tetrahydrofolate
- 669 binding (6S)-5,6,7,8-tetrahydrofolate
- 676:678 binding (6S)-5,6,7,8-tetrahydrofolate
- 737 binding (6S)-5,6,7,8-tetrahydrofolate
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
26% identity, 49% coverage: 31:239/430 of query aligns to 15:217/433 of 5hxwA
- active site: F58 (≠ S74), Q61 (= Q77), A62 (≠ I78)
- binding flavin-adenine dinucleotide: V22 (≠ I38), G23 (= G39), G25 (= G41), I26 (≠ V42), L27 (≠ T43), E46 (= E62), K47 (≠ A63), E53 (≠ G69), Q54 (≠ A70), S55 (= S71), R57 (= R73), F58 (≠ S74), Y59 (≠ G75), G60 (= G76), Q61 (= Q77), A188 (≠ P212), A189 (= A213)
Sites not aligning to the query:
- active site: 240, 284, 288
- binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 218, 219, 221, 240, 242, 331, 371, 373, 398, 399, 400, 401, 402, 403
Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
23% identity, 89% coverage: 32:414/430 of query aligns to 49:438/866 of Q9UI17
- CV 59:60 (≠ VT 42:43) binding FAD
- EK 80:81 (≠ EA 62:63) binding FAD
- 87:95 (vs. 69:77, 22% identical) binding FAD
- H91 (≠ R73) modified: Tele-8alpha-FAD histidine
- H109 (≠ S105) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
- V219 (≠ A213) binding FAD
- S279 (= S270) to P: in dbSNP:rs532964
- F-GYGII 397:402 (≠ YSGHGMS 369:375) binding FAD
Sites not aligning to the query:
- 530 A → G: in dbSNP:rs1805073
- 646 S → P: in dbSNP:rs1805074
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
22% identity, 84% coverage: 32:393/430 of query aligns to 1:349/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (= I38), G8 (= G39), G10 (= G41), V11 (= V42), I12 (≠ T43), V30 (≠ L61), E31 (= E62), K32 (≠ A63), E38 (≠ G69), A39 (= A70), S40 (= S71), A43 (≠ S74), G45 (= G76), L46 (= L79), V171 (≠ A213), G200 (≠ T240), G201 (≠ N241), W203 (≠ Y243), G298 (= G344), R300 (≠ V346), P301 (≠ A347), Y326 (≠ S370), R327 (≠ G371), N328 (≠ H372), G329 (= G373), I330 (≠ M374)
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
21% identity, 83% coverage: 48:404/430 of query aligns to 20:367/374 of 1y56B
- active site: F44 (≠ G72), G47 (= G75), T48 (≠ G76), H224 (≠ N261), P239 (≠ K276), G305 (= G344), M338 (≠ S375)
- binding flavin-adenine dinucleotide: I33 (≠ L61), E34 (= E62), K35 (≠ A63), S42 (≠ A70), T43 (≠ S71), R45 (= R73), C46 (≠ S74), G47 (= G75), G49 (≠ Q77), E170 (≠ P212), V171 (≠ A213), T200 (= T240), N201 (= N241), W203 (≠ Y243), G305 (= G344), Y306 (≠ D345), Y307 (≠ V346), G334 (= G371), H335 (= H372), G336 (= G373), F337 (≠ M374), M338 (≠ S375)
- binding flavin mononucleotide: F44 (≠ G72), R45 (= R73), I260 (≠ D299), R301 (≠ Y340), W303 (= W342)
Sites not aligning to the query:
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
23% identity, 83% coverage: 46:400/430 of query aligns to 18:361/369 of S5FMM4
- G51 (≠ L79) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- A54 (≠ L82) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- K81 (≠ V109) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
- S202 (≠ T240) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ M374) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L384) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
24% identity, 84% coverage: 35:396/430 of query aligns to 28:391/824 of Q8GAI3
- W66 (≠ G72) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (≠ R73) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
5fjnA Structure of l-amino acid deaminase from proteus myxofaciens in complex with anthranilate (see paper)
26% identity, 52% coverage: 17:239/430 of query aligns to 18:229/447 of 5fjnA
- active site: S67 (= S71), Y71 (≠ G75), S72 (≠ G76)
- binding flavin-adenine dinucleotide: I34 (= I38), G35 (= G39), G37 (= G41), I38 (≠ V42), Q39 (≠ T43), L57 (= L61), E58 (= E62), K59 (≠ A63), E65 (≠ G69), Q66 (≠ A70), S67 (= S71), A70 (≠ S74), Y71 (≠ G75), S72 (≠ G76), Q73 (= Q77), V201 (≠ A213)
Sites not aligning to the query:
- active site: 252
- binding 2-aminobenzoic acid: 252, 289, 411, 412
- binding flavin-adenine dinucleotide: 230, 231, 233, 252, 254, 343, 385, 410, 411, 412, 413, 414, 415
5fjmA Structure of l-amino acid deaminase from proteus myxofaciens (see paper)
26% identity, 52% coverage: 17:239/430 of query aligns to 18:229/447 of 5fjmA
- active site: S67 (= S71), Y71 (≠ G75), S72 (≠ G76)
- binding flavin-adenine dinucleotide: I34 (= I38), G35 (= G39), G37 (= G41), I38 (≠ V42), Q39 (≠ T43), L57 (= L61), E58 (= E62), K59 (≠ A63), E65 (≠ G69), Q66 (≠ A70), S67 (= S71), A70 (≠ S74), Y71 (≠ G75), S72 (≠ G76), Q73 (= Q77), V201 (≠ A213)
Sites not aligning to the query:
- active site: 252
- binding flavin-adenine dinucleotide: 230, 231, 233, 252, 254, 343, 385, 410, 411, 412, 413, 414, 415
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
22% identity, 82% coverage: 50:401/430 of query aligns to 22:359/364 of 3if9A
- active site: A47 (≠ G75), G48 (= G76), M49 (≠ Q77)
- binding flavin-adenine dinucleotide: E34 (= E62), S35 (≠ A63), T42 (≠ A70), T43 (≠ S71), A46 (≠ S74), A47 (≠ G75), G48 (= G76), M49 (≠ Q77), P173 (= P212), V174 (≠ A213), S202 (≠ T240), G203 (≠ N241), W205 (≠ Y243), F209 (vs. gap), G300 (= G344), R302 (≠ V346), H327 (≠ Y369), F328 (≠ S370), R329 (≠ G371), N330 (≠ H372), G331 (= G373), I332 (≠ M374)
- binding glycolic acid: Y246 (≠ F289), R302 (≠ V346), R329 (≠ G371)
Sites not aligning to the query:
Query Sequence
>WP_043526905.1 NCBI__GCF_000759345.1:WP_043526905.1
MTSAALHRPASYYQDSIAVTAGPFPALEESRRADVCIIGGGVTGCSAALHLAERGYSVVL
LEAAEVGYGASGRSGGQILPGLGTDIATVEKALGKSRARDIWEMSRESVRLTAALIERHG
IPCDLAWGYLHAAVKSRHVEELKAFQARLATDYDYPALQWLEGASLQEHVVTNAYPGALY
DAEGGHLHPLNYTLGLARAAQHAGVRIHEHSPAVEVHPGQPAQVATPGGRVTADYVIVST
NAYRGEGLLPDLDGRIMRAANYMIATEPLSEAQVAKVLPSNDALSDANFVLDYYRLSADR
RLIYGGEVSYDGREPRQLRERMDAKMARLFPVLEGIGIDYRWGGDVAITLNRAPDFGRVG
SNVYYAHGYSGHGMSLAGLAGQLLAETISGQSERFDIFAAMPHRSFPGGKWLRTPLLVLA
TQFYKLRDRL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory