SitesBLAST
Comparing WP_043527041.1 NCBI__GCF_000759345.1:WP_043527041.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
47% identity, 99% coverage: 1:351/354 of query aligns to 1:365/369 of P19566
- L86 (= L87) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P161) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D166) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (= E294) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
48% identity, 97% coverage: 1:343/354 of query aligns to 1:357/371 of P68187
- A85 (= A86) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (= K107) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V115) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ A118) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ A120) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E125) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G138) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D159) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ M229) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ M242) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ L256) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (vs. gap) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (vs. gap) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (= G268) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G288) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (= E294) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
- S322 (≠ G308) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G340 (= G326) mutation to A: Maltose transport is affected but retains ability to interact with MalT.
- G346 (≠ H332) mutation to S: Normal maltose transport but constitutive mal gene expression.
- F355 (= F341) mutation to Y: Maltose transport is affected but retains ability to interact with MalT.
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
50% identity, 86% coverage: 1:304/354 of query aligns to 4:331/375 of 2d62A
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
52% identity, 87% coverage: 1:307/354 of query aligns to 1:325/393 of P9WQI3
- H193 (= H193) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
48% identity, 97% coverage: 2:343/354 of query aligns to 1:356/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
48% identity, 97% coverage: 2:343/354 of query aligns to 1:356/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y13), S37 (= S39), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), Q81 (= Q83), R128 (= R130), A132 (≠ K134), S134 (= S136), G136 (= G138), Q137 (= Q139), E158 (= E160), H191 (= H193)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
48% identity, 97% coverage: 2:343/354 of query aligns to 1:356/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y13), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R130), S134 (= S136), Q137 (= Q139)
- binding beryllium trifluoride ion: S37 (= S39), G38 (= G40), K41 (= K43), Q81 (= Q83), S134 (= S136), G136 (= G138), H191 (= H193)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
48% identity, 97% coverage: 2:343/354 of query aligns to 1:356/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R130), A132 (≠ K134), S134 (= S136), Q137 (= Q139)
- binding tetrafluoroaluminate ion: S37 (= S39), G38 (= G40), K41 (= K43), Q81 (= Q83), S134 (= S136), G135 (= G137), G136 (= G138), E158 (= E160), H191 (= H193)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
48% identity, 97% coverage: 2:343/354 of query aligns to 1:356/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y13), V17 (≠ A19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (= R130), A132 (≠ K134), S134 (= S136), Q137 (= Q139)
- binding magnesium ion: S42 (= S44), Q81 (= Q83)
1g291 Malk (see paper)
47% identity, 98% coverage: 1:347/354 of query aligns to 1:369/372 of 1g291
- binding magnesium ion: D69 (= D70), E71 (= E72), K72 (vs. gap), K79 (≠ A74), D80 (≠ E75), E292 (≠ D272), D293 (= D273), K359 (≠ A337)
- binding pyrophosphate 2-: S38 (= S39), G39 (= G40), C40 (= C41), G41 (= G42), K42 (= K43), T43 (≠ S44), T44 (= T45)
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
48% identity, 96% coverage: 4:343/354 of query aligns to 1:354/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ Y13), S35 (= S39), G36 (= G40), C37 (= C41), G38 (= G42), K39 (= K43), S40 (= S44), T41 (= T45), R126 (= R130), A130 (≠ K134), S132 (= S136), G134 (= G138), Q135 (= Q139)
8hprD Lpqy-sugabc in state 4 (see paper)
48% identity, 97% coverage: 2:346/354 of query aligns to 1:356/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (= Y13), S38 (= S39), C40 (= C41), G41 (= G42), K42 (= K43), S43 (= S44), T44 (= T45), Q82 (= Q83), R129 (= R130), Q133 (≠ K134), S135 (= S136), G136 (= G137), G137 (= G138), Q159 (≠ E160), H192 (= H193)
- binding magnesium ion: S43 (= S44), Q82 (= Q83)
8hprC Lpqy-sugabc in state 4 (see paper)
48% identity, 97% coverage: 2:346/354 of query aligns to 1:357/363 of 8hprC