SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043527315.1 NCBI__GCF_000759345.1:WP_043527315.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
49% identity, 97% coverage: 2:422/432 of query aligns to 3:423/426 of P22256

query
sites
P22256

N

S

N

N

A

K

Q

E

L

L

N

M

E

Q

L

R

K

R

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R

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Y

A

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H

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F

A

A

D

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C

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W

D

D

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G

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Y

I

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F

A

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I
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I

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A

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V

L

L

N

N

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I50 (= I49) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
GS 111:112 (≠ GA 110:111) binding pyridoxal 5'-phosphate
E211 (= E210) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
V241 (= V240) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
Q242 (= Q241) binding pyridoxal 5'-phosphate
K268 (= K267) modified: N6-(pyridoxal phosphate)lysine
T297 (= T296) binding pyridoxal 5'-phosphate

1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
49% identity, 97% coverage: 2:422/432 of query aligns to 2:422/425 of 1sffA

query
sites
1sffA

N

S

N

N

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x
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A

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A

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E

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N

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G

A
x
S

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E

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A

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E

N

N

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A

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A

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A

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K

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I

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A

F

F

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A

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Y

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active site: V18 (≠ A18), Y137 (= Y137), E205 (= E205), D238 (= D238), Q241 (= Q241), K267 (= K267), T296 (= T296), R397 (= R397)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (≠ T78), G110 (= G110), S111 (≠ A111), Y137 (= Y137), H138 (= H138), R140 (= R140), E205 (= E205), D238 (= D238), V240 (= V240), Q241 (= Q241), K267 (= K267), T296 (= T296)
binding sulfate ion: N152 (≠ A152), Y393 (≠ G393)

1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
49% identity, 97% coverage: 2:422/432 of query aligns to 2:422/425 of 1sf2A

query
sites
1sf2A

N

S

N

N

A

K

Q

E

L

L

N

M

E

Q

L

R

K

R

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S

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Q

Y

A

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I

A

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G

G

A
x
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Q

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I

A

H

T

P

Q

I

F

F

A

A

D

D

R

R

A

A

E

E

N

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A

C

L

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I

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W

W

D

D

A

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D

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G

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Y

I

L

D

D

F

F

A

A

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A

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L

N

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G

G

H

H

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H

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K

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A

A

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E

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A

I

Q

L

I

E

R

E

Q

G

G

L

L

G

E

V

I

E

S

S

Q

A

C

L

F

K

D

E

E

active site: V18 (≠ A18), Y137 (= Y137), E205 (= E205), D238 (= D238), Q241 (= Q241), K267 (= K267), T296 (= T296), R397 (= R397)
binding pyridoxal-5'-phosphate: G110 (= G110), S111 (≠ A111), Y137 (= Y137), H138 (= H138), E205 (= E205), D238 (= D238), V240 (= V240), Q241 (= Q241), K267 (= K267)
binding sulfate ion: N152 (≠ A152), Y393 (≠ G393)

1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
49% identity, 97% coverage: 2:422/432 of query aligns to 2:422/425 of 1szkA

query
sites
1szkA

N

S

N

N

A

K

Q

E

L

L

N

M

E

Q

L

R

K

R

Q

S

R

Q

Y

A

V

I

A

P

N

R

G

G

A
x
V

A

G

S

Q

P

I

A

H

T

P

Q

I

F

F

A

A

D

D

R

R

A

A

E

E

N

N

A

C

L

R

I

V

W

W

D

D

A

V

D

E

G

G

N

R

R

E

L

Y

I

L

D

D

F

F

A

A

G

G

I

I

G

A

V

V

L

L

N

N

I

T

G

G

H

H

R

L

H

H

P

P

K

K

V

V

E

A

A

A

V

V

K

E

A

A

Q

Q

L

L

D

K

K

K

V

L

M

S

H

H

T

T

C

C

Q

F

T

Q

V

V

M

L

P

A

Y

Y

E

E

G

P

Y

Y

V

L

K

E

V

L

A

C

E

E

K

I

L

M

S

N

Q

Q

I

K

T

V

P

P

V

G

R

D

G

F

H

A

A

K

K

K

V

T

M

L

L

L

A

V

N

T

S

T

G
|
G

A
x
S

E

E

A

A

L

V

E

E

N

N

A

A

V

V

K

K

V

I

A

A

R

R

A

A

T

T

G

K

K

R

N

S

N

G

V

T

I

I

C

A

F

F

D

S

G

G

G

A

Y
|
Y

H
|
H

G

G

R

R

T

T

F

H

M

Y

T

T

M

L

A

A

M

L

N

T

G

G

K

K

V

V

A

N

P

P

Y

Y

A

S

T

A

D

G

F

M

G

G

S

L

M

M

P

P

G

G

N

H

V

V

F

Y

R

R

A

A

P

L

Y

Y

P

P

V

C

P

P

Y

L

H

H

G

G

V

I

S

S

E

E

D

D

E

D

A

A

L

I

R

A

G

S

L

I

K

H

M

R

A

I

L

F

K

K

T

N

D

D

A

A

N

A

P

P

K

E

D

D

T

I

A

A

I

I

V

V

L

I

E
|
E

P

P

V

V

L

Q

G

G

E

S

G

G

F

F

Y

Y

A

A

A

S

P

S

A

P

S

A

F

F

L

M

K

Q

A

R

I

L

R

R

D

A

I

L

C

C

D

D

E

E

H

H

G

G

I

I

L

M

L

L

I

I

I

A

D
|
D

E

E

V
|
V

Q
|
Q

S

S

G

G

F

A

G

G

R

R

T

T

G

G

K

T

M

L

F

F

A

A

I

M

E

E

H

Q

S

M

G

G

V

V

E

A

P

P

D

D

I

L

I

T

C

T

M

F

A

A

K
|
K

S

S

M

I

A

A

D

G

G

G

M

F

P

P

I

L

S

A

A

G

V

V

V

T

G

G

T

R

D

A

K

E

V

V

M

M

D

D

A

A

S

V

G

A

G

P

N

G

S

G

L

L

G

G

T
|
T

Y

Y

T

A

G

G

S

N

P

P

V

I

S

A

C

C

A

V

A

A

T

A

L

L

A

E

V

V

L

L

E

K

V

V

F

F

E

E

E

Q

E

E

K

N

I

L

L

L

E

Q

K

K

S

A

Q

N

A

D

L

L

G

G

D

Q

K

K

L

L

A

K

K

D

R

G

F

L

S

L

Q

A

W

I

E

A

Q

E

D

K

F

H

D

P

C

E

V

I

D

G

N

D

G

V

R

R

N

G

L

L

G

G

A

A

M

M

A

I

A

A

F

I

D

E

L

L

V

F

S

E

D

D

K

G

A

D

Q

H

H

N

T

K

P

P

D

D

A

A

D

K

L

L

A

T

G

A

A

E

L

I

C

V

K

A

R

R

A

A

R

R

E

D

K

K

G

G

L

L

V

I

L

L

S

S

C

C

G

G

L

P

Y

Y

G

Y

N

N

T

V

I

L

R
|
R

F

I

L

L

M

V

P

P

V

L

T

T

I

I

E

E

D

D

D

A

I

Q

L

I

E

R

E

Q

G

G

L

L

G

E

V

I

E

S

S

Q

A

C

L

F

K

D

E

E

active site: V18 (≠ A18), Y137 (= Y137), E205 (= E205), D238 (= D238), Q241 (= Q241), K267 (= K267), T296 (= T296), R397 (= R397)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G110), S111 (≠ A111), Y137 (= Y137), H138 (= H138), E205 (= E205), D238 (= D238), V240 (= V240), Q241 (= Q241), K267 (= K267)

P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
46% identity, 98% coverage: 1:422/432 of query aligns to 1:421/421 of P50457

query
sites
P50457

M

M

N

S

N

N

A

N

Q

E

L

F

N

H

E

Q

L

R

K

R

Q

L

R

S

Y

A

V

T

A

P

N

R

G

G

A

V

A

G

S

V

P

M

A

C

T

N

Q

F

F

F

A

A

D

Q

R

S

A

A

E

E

N

N

A

A

L

T

I

L

W

K

D

D

A

V

D

E

G

G

N

N

R

E

L

Y

I

I

D

D

F

F

A

A

G

A

G

G

I

I

G

A

V

V

L

L

N

N

I

T

G

G

H

H

R

R

H

H

P

P

K

D

V

L

V

V

E

A

A

A

V

V

K

E

A

Q

Q

Q

L

L

D

Q

K

Q

V

F

M

T

H

H

T

T

C

A

Q

Y

T

Q

V

I

M

V

P

P

Y

Y

E

E

G

S

Y

Y

V

V

K

T

V

L

A

A

E

E

K

K

L

I

S

N

Q

A

I

L

T

A

P

P

V

V

R

S

G

G

H

Q

A

A

K

K

V

T

M

A

L

F

A

F

N

T

S

T

G

G

A

A

E

E

A

A

L

V

E

E

N

N

A

A

V

V

K

K

V

I

A

A

R

R

A

A

A

H

T

T

G

G

K

R

N

P

N

G

V

V

I

I

C

A

F

F

D

S

G

G

Y

F

H

H

G

G

R

R

T

T

F

Y

M

M

T

T

M

M

A

A

M

L

N

T

G

G

K

K

V

V

A

A

P

P

Y

Y

A

K

T

I

D

G

F

F

G

G

S

P

M

F

P

P

G

G

N

S

V

V

F

Y

R

H

A

V

P

P

Y

Y

P

P

V

S

P

D

Y

L

H

H

G

G

V

I

S

S

E

T

D

Q

E

D

A

S

L

L

R

D

G

A

L

I

K

E

M

R

A

L

L

F

K

K

T

S

D

D

A

I

N

E

P

A

K

K

D

Q

T

V

A

A

I

I

V

I

L

F

E

E

P

P

V

V

L

Q

G

G

E

E

G

G

F

F

Y

N

A

V

A

A

P

P

A

K

S

E

F

L

L

V

K

A

A

A

I

I

R

R

D

R

I

L

C

C

D

D

E

E

H

H

G

G

I

I

L

V

L

M

I

I

I

A

D

D

E

E

V

V

Q

Q

S

S

G

G

F

F

G

A

R

R

T

T

G

G

K

K

M

L

F

F

A

A

I

M

E

D

H

H

S

Y

G

A

V

D

E

K

P

P

D

D

I

L

I

M

C

T

M

M

A

A

K
|
K

S

S

M

L

A

A

D

G

G

G

M

M

P

P

I

L

S

S

A

G

V

V

G

G

T

N

D

A

K

N

V

I

M

M

D

D

A

A

S

P

G

A

G

P

N

G

S

G

L

L

G

G

T

T

Y

Y

T

A

G

G

S

N

P

P

V

L

S

A

C

V

A

A

T

A

L

H

A

A

V

V

L

L

E

N

V

I

F

I

E

D

E

K

E

E

K

S

I

L

L

C

E

E

K

R

S

A

Q

N

A

Q

L

L

G

G

D

Q

K

R

L

L

A

K

K

N

R

T

F

L

S

I

Q

D

W

A

E

K

Q

E

D

S

F

V

D

P

C

A

V

I

D

A

N

A

G

V

R

R

N

G

L

L

G

G

A

S

M

M

A

I

A

A

F

V

D

E

L

F

V

-

S

N

D

D

K

P

A

Q

Q

T

H

G

T

E

P

P

D

S

A

A

D

A

L

I

A

A

G

Q

A

K

L

I

C

Q

K

Q

R

R

A

A

R

L

E

A

K

Q

G

G

L

L

V

L

L

L

S

T

C

C

G

G

L

A

Y

Y

G

G

N

N

T

V

I

I

R

R

F

F

L

L

M

Y

P

P

V

L

T

T

I

I

E

P

D

D

D

A

I

Q

L

F

E

D

E

A

G

A

L

M

G

K

V

I

V

L

E

Q

S

D

A

A

L

L

K

S

E

D

K267 (= K267) mutation to A: No GABA-AT activity.

3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
42% identity, 97% coverage: 5:423/432 of query aligns to 19:438/439 of 3q8nC

query
sites
3q8nC

Q

E

L

L

N

Q

E

A

L

R

K

K

Q

Q

R

S

Y

A

V

V

A

A

N

A

G

G

A
x
V

A

G

S

V

P

T

A

L

T

P

Q

V

F

Y

A

V

D

V

R

A

A

A

E

G

N

G

A

G

L

V

I

L

W

A

D

D

A

A

D

D

G

G

N

N

R

Q

L

L

I

I

D

D

F

F

A

G

G

S

G

G

I

I

G

A

V

V

L

T

N

T

I

V

G

G

H

N

R

S

H

A

P

P

K

A

V

V

E

D

A

A

V

V

K

T

A

Q

Q

Q

L

V

D

A

K

A

V

F

M

T

H

H

T

T

C

C

Q

F

T

M

V

V

M

T

P

P

Y

Y

E

E

G

G

Y

Y

V

V

K

K

V

V

A

A

E

E

K

H

L

L

S

N

Q

R

I

L

T

T

P

P

V

G

R

D

G

H

H

E

A

K

K

R

V

T

M

A

L

L

A

F

N

N

S

S

G
|
G

A
|
A

E

E

A

A

L

V

E

E

N

N

A

A

V

V

K

K

V

I

A

A

R

R

A

A

A

Y

T

T

G

R

K

R

N

Q

N

A

V

V

I

V

C

V

F

F

D

D

G

H

G

A

Y
|
Y

H

H

G

G

R

R

T

T

F

N

M

L

T

T

M

M

A

A

M

M

N

T

G

A

K

K

V

N

A

Q

P

P

Y

Y

A

K

T

H

D

G

F

F

G

G

S

P

M

F

P

A

G

N

N

E

V

V

F

Y

R

R

A

V

P

P

Y

T

P

S

V

Y

P

P

Y

F

H

R

-

D

G

G

V

E

S

T

E

D

D

G

E

A

A

A

L

A

R

A

G

A

L

H

K

A

M

L

A

D

L

L

-

I

K

N

T

K

D

Q

A

V

N

G

P

A

K

D

D

N

T

V

A

A

I

V

V

V

L

I

E
|
E

P

P

V

V

L

H

G

G

E

E

G

G

F

F

Y

V

A

V

A

P

P

A

A

P

S

G

F

F

L

L

K

G

A

A

I

L

R

Q

D

K

I

W

C

C

D

T

E

D

H

N

G

G

I

A

L

V

L

F

I

V

I

A

D
|
D

E

E

V
|
V

Q
|
Q

S

T

G

G

F

F

G

A

R

R

T

T

G

G

K

A

M

L

F

F

A

A

I

C

E

E

H

H

S

E

G

N

V

V

E

V

P

P

D

D

I

L

I

I

C

V

M

T

A

A

K
|
K

S

G

M

I

A

A

D

G

G

G

M

L

P

P

I

L

S

S

A

A

V

V

V

T

G

G

T

R

D

A

K

E

V

I

M

M

D

D

A

G

S

P

G

Q

G

S

N

G

S

G

L

L

G

G

T
|
T

Y

Y

T

G

G

G

S

N

P

P

V

L

S

A

C

C

A

A

T

A

L

L

A

A

V

V

L

I

E

D

V

T

F

I

E

E

E

R

E

E

K

N

I

L

L

V

E

A

K

R

S

A

Q

R

A

A

L

I

G

G

D

E

K

T

L

M

A

L

K

S

R

R

F

L

S

G

Q

A

W

L

E

A

Q

A

D

A

F

D

D

P

C

R

V

I

D

G

N

E

G

V

R

R

N

G

L

R

G

G

A

A

M

M

A

I

A

A

F

V

D

E

L

L

V

V

S

K

D

P

K

G

A

T

Q

T

H

E

T

-

P

P

D

D

A

A

D

D

L

L

A

T

G

K

A

R

L

V

C

A

K

A

R

A

A

A

R

H

E

A

K

Q

G

G

L

L

V

V

L

V

L

L

S

T

C

C

G

G

L

T

Y

Y

G

G

N

N

T

V

I

L

R
|
R

F

F

L

L

M

P

P

P

V

L

T

S

I

M

E

P

D

D

D

H

I

L

L

L

E

D

E

E

G

G

L

L

G

D

V

I

V

L

E

A

S

A

A

V

L

F

K

A

E

E

L

V

active site: V32 (≠ A18), Y151 (= Y137), E221 (= E205), D254 (= D238), Q257 (= Q241), K283 (= K267), T312 (= T296), R412 (= R397)
binding 4-oxobutanoic acid: G124 (= G110), A125 (= A111), V256 (= V240), K283 (= K267)

6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
44% identity, 97% coverage: 6:422/432 of query aligns to 23:437/440 of 6j2vA

query
sites
6j2vA

L

L

N

D

E

E

L

R

K

R

Q

Q

R

A

Y

A

V

V

A

A

N

R

G

A

A
x
L

A

A

S

P

P

G

A

L

T

P

Q

G

F

Y

A

V

D

V

R

D

A

A

E

D

N

G

A

G

L

I

I

L

W

A

D

D

A

A

D

D

G

G

N

N

R

R

L

F

I

I

D

D

F

L

A

A

G

S

G

G

I

I

G

A

V

V

L

T

N

T

I

V

G

G

H

G

R

S

H

N

P

A

K

A

V

V

V

A

E

K

A

A

V

V

K

G

A

A

Q

A

L

A

D

A

K

R

V

F

M

T

H

H

T

T

C

C

Q

F

T

M

V

V

M

S

P

P

Y

Y

E

E

G

T

Y

Y

V

V

K

A

V

M

A

A

E

E

K

R

L

L

S

N

Q

A

I

L

T

T

P

P

V

G

R

D

G

H

H

D

A

K

K

K

V

S

M

A

L

L

A

F

N

N

S

S

G
|
G

A
|
A

E

E

A

A

L

V

E

E

N

N

A

A

V

V

K

K

V

V

A

A

R

R

A

A

A

Y

T

T

G

G

K

K

N

G

N

A

V

V

I

V

C

V

F

F

D

D

G

N

G

A

Y
|
Y

H
|
H

G

G

R
|
R

T

T

F

N

M

L

T

T

M

M

A

A

M

M

N

T

G

A

K

K

V

N

A

R

P

P

Y

Y

A

K

T

S

D

G

F

F

G

G

S

P

M

L

P

A

G

A

N

D

V

V

F

Y

R

R

A

A

P

P

Y

M

P

S

V

Y

P

P

Y

L

H

R

-

D

G

G

V

L

S

S

E

G

D

P

E

E

A

A

L

A

R

E

G

R

L

A

K

I

M

S

A

V

L

I

K

E

T

S

D

Q

A

V

N

G

P

A

K

E

D

N

T

L

A

A

A

C

I

V

V

V

L

I

E
|
E

P

P

V

I

L

Q

G

G

E
|
E

G

G

F

F

Y

I

A

V

A

P

P

A

A

P

S

G

F

F

L

L

K

A

A

A

I

I

R

S

D

T

I

W

C

C

D

R

E

E

H

N

G

D

I

V

L

V

L

F

I

I

I

A

D
|
D

E

E

V
x
I

Q

Q

S

S

G

G

F

F

G

L

R

R

T

T

G

G

K

D

M

W

F

F

A

A

I

S

E

D

H

A

S

E

G

G

V

V

E

I

P

P

D

D

I

V

I

I

C

T

M

T

A

A

K
|
K

S

G

M

I

A

A

D

G

G

G

M

M

P

P

I

L

S

S

A

A

V

V

V

T

G

G

T

R

D

A

K

E

V

I

M

M

D

D

A

A

S

P

G

G

G

P

N

G

S

A

L

L

G

G

G
|
G

T
|
T

Y

Y

T

G

G

G

S

N

P

P

V

V

S

A

C

C

A

A

T

A

L

L

A

A

V

A

L

I

E

E

V

V

F

M

E

E

E

Q

E

A

K

D

I

L

L

K

E

T

K

R

S

A

Q

Q

A

E

L

I

G

E

D

T

K

I

L

I

A

R

K

D

R

E

F

F

S

A

Q

Q

W

L

E

S

Q

A

D

-

F

F

D

P

C

E

V

V

D

A

N

E

G

I

R

R

N

G

L

R

G

G

A

A

M

M

A

M

A

A

F

I

D

E

L

L

V

I

S

D

D

-

K

-

A

A

Q

T

H

G

T

R

P

P

D

N

A

A

D

A

L

L

A

T

G

A

A

A

L

V

C

A

K

A

R

R

A

A

R

K

E

A

K

E

G

G

L

V

V

L

L

L

S

T

C

C

G

G

L

T

Y

D

G

G

N

N

T

V

I

I

R

R

F

L

L

L

M

P

P

P

V

L

T

V

I

I

E

A

D

E

D

D

I

T

L

L

E

R

E

D

G

G

L

L

G

Q

V

V

V

L

E

V

S

A

A

A

L

L

K

E

E

R

active site: L35 (≠ A18), Y154 (= Y137), D256 (= D238), K285 (= K267)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G110), A128 (= A111), Y154 (= Y137), H155 (= H138), R157 (= R140), E223 (= E205), E228 (= E210), D256 (= D238), I258 (≠ V240), K285 (= K267), G313 (= G295), T314 (= T296)

4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
41% identity, 96% coverage: 6:421/432 of query aligns to 23:442/444 of 4atqF

query
sites
4atqF

L

L

N

A

E

E

L

R

K

R

Q

S

R

A

Y

V

V

V

A

A

N

A

G

G

A
x
V

A

A

S

S

P

G

A

V

T

P

Q

V

F

Y

A

V

D

E

R

D

A

A

E

D

N

G

A

G

L

I

I

I

W

R

D

D

A

V

D

D

G

G

N

N

R

S

L

F

I

I

D

D

F

L

A

G

G

S

G

G

I

I

G

A

V

V

L

T

N

S

I

V

G

G

H

A

R

S

H

D

P

P

K

A

V

V

E

A

A

A

V

V

K

Q

A

E

Q

A

L

A

D

A

K

H

V

F

M

T

H

H

T

T

C

C

Q

F

T
x
M

V

V

M

T

P

P

Y

Y

E

E

G

G

Y

Y

V

V

K

A

V

V

A

T

E

E

K

Q

L

L

S

N

Q

R

I

L

T

T

P

P

V

G

R

D

G

H

H

A

A

K

K

R

V

T

M

V

L

L

A

F

N

N

S

S

G
|
G

A
|
A

E

E

A

A

L

V

E

E

N

N

A

A

V

V

K

K

V

V

A

A

R

R

A

L

A

A

T

T

G

G

K

R

N

D

N

A

V

V

I

V

C

A

F

F

D

D

G

H

G

A

Y
|
Y

H
|
H

G

G

R
|
R

T

T

F

N

M

L

T

T

M

M

A

A

M

L

N

T

G

A

K

K

V

A

A

M

P

P

Y

Y

A

K

T

T

D

N

F

F

G

G

S

P

M

F

P

A

G

P

N

E

V

V

F

Y

R

R

A

M

P

P

Y

M

P

S

V

Y

P

P

Y

F

H

R

-

E

-

N

-

P

G

E

V

I

S

T

E

G

D

A

E

E

A

A

L

A

R

K

G

R

L

A

K

I

M

T

A

M

L

I

K

E

T

K

D

Q

A

I

N

G

P

G

K

D

D

Q

T

V

A

A

I

I

V

I

L

I

E
|
E

P

P

V

I

L

Q

G

G

E
|
E

G

G

F

F

Y

I

A

V

A

P

P

A

A

E

S

G

F

F

L

L

K

P

A

A

I

L

R

S

D

E

I

W

C

A

D

K

E

E

H

K

G

G

I

I

L

V

L

F

I

I

I

A

D
|
D

E

E

V
|
V

Q
|
Q

S

S

G

G

F

F

G

C

R

R

T

T

G

G

K

E

M

W

F

F

A

A

I

V

E

D

H

H

S

E

G

G

V

V

E

V

P

P

D

D

I

I

C

T

M

M

A

A

K
|
K

S

G

M

I

A

A

D

G

G

G

M

L

P

P

I

L

S

S

A

A

V

I

V

T

G

G

T

R

D

A

K

D

V

L

M

L

D

D

A

A

S

V

G

H

G

P

N

G

S

G

L

L

G

G

G
|
G

T
|
T

Y

Y

T

G

G

G

S

N

P

P

V

V

S

A

C

C

A

A

T

A

L

L

A

A

V

A

L

I

E

D

V

T

F

M

E

E

E

Q

E

H

K

D

I

L

L

N

E

G

K

R

S

A

Q

R

A

H

L

I

G

E

D

E

-

L

K

A

L

L

A

G

K

K

R

L

F

R

S

E

Q

L

W

A

E

A

Q

E

D

G

F

G

D

S

C

V

V

V

D

G

N

D

G

I

R

R

N

G

L

R

G

G

A

A

M

M

A

L

A

A

F

I

D

E

L

L

V

V

S

Q

D

P

K

G

A

S

Q

K

H

E

T

-

P

P

D

N

A

A

D

E

L

L

A

T

G

K

A

A

L

V

C

A

K

A

R

A

A

C

R

L

E

K

K

E

G

G

L

V

V

I

L

I

L

L

S

T

C

C

G

G

L

T

Y

Y

G

G

N

N

T

V

I

I

R
|
R

F

L

L

L

M

P

P

P

V

L

T

V

I

I

E

S

D

D

D

E

I

L

L

L

E

I

E

D

G

G

L

L

G

E

V

V

V

L

E

A

S

A

A

A

L

I

K

K

active site: V35 (≠ A18), Y154 (= Y137), E226 (= E205), D259 (= D238), Q262 (= Q241), K288 (= K267), T317 (= T296), R418 (= R397)
binding gamma-amino-butanoic acid: M95 (≠ T78), Y154 (= Y137), R157 (= R140), E231 (= E210), K288 (= K267), G316 (= G295)
binding pyridoxal-5'-phosphate: G127 (= G110), A128 (= A111), Y154 (= Y137), H155 (= H138), D259 (= D238), V261 (= V240)

O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
34% identity, 93% coverage: 28:428/432 of query aligns to 58:466/474 of O58478

query
sites
O58478

R

R

A

G

E

F

N

G

A

P

L

F

I

I

W

E

D

D

A

V

D

D

G

G

N

N

R

V

L

F

I

I

D

D

F

F

A

L

G

A

G

G

I

A

G

A

V

A

L

A

N

S

I

T

G

G

H

Y

R

A

H

H

P

P

K

K

V

L

V

V

E

K

A

A

V

V

K

K

A

E

Q

Q

L

V

D

E

K

L

V

I

M

Q

H

H

T

S

C

M

Q

I

T

G

V

Y

M

T

P

H

Y

S

E

E

G

R

Y

A

V

I

K

R

V

V

A

A

E

E

K

K

L

L

S

V

Q

E

I

I

T

S

P

P

V

I

R

E

G

-

H

N

A

S

K

K

V

V

M

I

L

F

A

G

N

L

S

S

G

G

A

S

E

D

A

A

L

V

E

D

N

M

A

A

V

I

K

K

V

V

A

S

R

K

A

F

A

S

T

T

G

R

K

R

N

P

N

W

V

I

I

L

C

A

F

F

D

I

G

G

G

A

Y

Y

H

H

G

G

R

Q

T

T

F

L

M

G

T

A

M

T

A

S

M

V

N

A

G

S

K

F

V

Q

A

V

P

S

Y

Q

A

K

T

R

D

G

F

Y

G

S

S

P

M

L

P

M

G

P

N

N

V

V

F

F

R

W

A

I

P

P

Y

Y

P

P

V

N

P

P

Y

F

H

R

G

N

V

I

-

W

-

G

-

I

-

N

-

G

-

Y

-

E

S

E

E

P

D

D

E

E

-

L

-

I

-

N

-

R

A

V

L

L

R

D

G

Y

L

L

K

E

M

Y

A

Y

L

V

K

F

T

S

D

H

A

V

N

V

P

P

K

P

D

D

-

E

T

V

A

A

I

L

V

F

L

A

E

E

P

P

V

I

L

Q

G

G

E
x
D

G

A

G

G

F

I

Y

V

A

V

A

P

P

P

A

E

S

N

F

F

L

F

K

K

A

E

I

L

R

K

D

K

I

L

C

L

D

E

E

E

H

Y

G

G

I

I

L

L

I

V

I

M

D

D

E

E

V

V

Q

Q

S

T

G

G

F

I

G

G

R

R

T

T

G

G

K

K

M

W

F

F

A

A

I

S

E

E

H

W

S

F

G

N

V

V

E

K

P

P

D

D

I

M

I

I

C

I

M

F

A

G

K
|
K

S

G

M

V

A

A

D

S

G

G

M

M

P

G

I

L

S

S

A

G

V

V

V

I

G

G

T

R

D

K

K

E

V

I

M

M

D

D

A

I

S

T

G

S

G

G

N

S

S

A

L

L

G

-

G

L

T

T

Y

P

T

A

G

A

S

N

P

P

V

V

S

I

C

S

A

A

T

A

L

E

A

A

V

T

L

L

E

E

V

I

F

I

E

E

K

N

I

L

L

L

E

K

K

N

S

A

Q

L

A

E

L

V

G

G

D

E

K

F

L

I

A

M

K

G

R

R

F

L

S

K

Q

E

W

I

E

K

Q

E

D

R

F

F

D

E

C

I

V

I

D

G

N

D

G

V

R

R

N

G

L

K

G

G

A

L

M

M

A

I

A

G

F

V

D

E

L

I

V

V

S

K

D

E

K

N

A

G

Q

R

H

-

T

-

P

P

D

D

A

P

D

E

L

M

A

T

G

G

A

K

L

I

C

C

K

W

R

R

A

A

R

F

E

E

K

L

G

G

L

L

V

I

L

L

L

P

S

S

C

Y

G

G

L

M

Y

F

G

G

N

N

T

V

I

I

R

R

F

I

L

T

M

P

P

P

V

L

T

V

I

L

E

T

D

K

D

E

I

V

L

A

E

E

E

K

G

A

L

L

G

E

V

I

E

E

S

R

A

A

L

I

K

K

E

D

L

T

V

L

G

T

N

G

K

K

A

V

D251 (≠ E210) mutation to A: Loss of activity.
K308 (= K267) mutation to A: Loss of activity.

O50131 Ornithine aminotransferase; Orn-AT; Ornithine delta-aminotransferase; EC 2.6.1.13 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
37% identity, 96% coverage: 8:421/432 of query aligns to 20:443/454 of O50131

query
sites
O50131

E

E

L

E

K

H

Q

H

R

K

Y

Y

V

M

A

A

N

T

G

T

A

T

A

N

S

D

P

P

A

N

T

E

Q

Y

F

F

-

L

-

V

A

I

D

E

R

R

A

A

E

E

N

G

A

V

L

Y

I

W

W

I

D

D

A

V

D

D

G

G

N

N

R

V

L

L

I

L

D

D

F

F

A

S

G

S

G

G

I

I

G

G

V

V

L

M

N

N

I

V

G

G

H

L

R

R

H

N

P

P

K

K

V

V

V

I

E

E

A

A

V

I

K

K

A

K

Q

Q

L

L

D

D

K

L

V

V

M

L

H

H

T

A

C

A

Q

G

T
|
T

-
x
D

-

Y

V

Y

M

N

P

P

Y

Y

E

Q

G

-

Y

-

V

V

K

E

V

L

A

A

E

K

K

K

L

L

S

V

Q

E

I

I

T

A

P

P

V

G

R

D

G

I

H

E

A

R

K

K

V

V

M

F

L

L

A

S

N

N

S

S

G
|
G

A
x
T

E

E

A

A

L

N

E

E

N

A

A

A

V

L

K

K

V

I

A

A

R

K

A

W

A

S

T

T

G

N

K

R

N

K

N

M

V

F

I

I

C

A

F

F

D

I

G

G

G

A

Y

F

H

H

G

G

R

R

T

T

F

H

M

G

T

T

M

M

A

S

M

L

N

T

-

A

G

S

K

K

V

P

A

V

P

Q

Y

R

A

S

T

R

D

M

F

F

G

P

S

T

M

M

P

P

G

G

N

-

V

V

F

V

R

H

A

V

P

P

Y

Y

P

P

V

N

P

P

Y

Y

H

R

-

N

-

P

-

W

G

G

V

I

-

D

-

G

-

Y

-

E

S

N

E

P

D

D

E

E

A

L

L

I

R

N

G

R

L

V

-

I

-

D

-

Y

-

I

-

E

K

E

M

Y

A

L

L

F

K

E

T

H

D

Y

A

V

N

P

P

A

K

E

D

E

T

V

A

A

A

G

I

I

V

F

L

F

E

E

P

P

V

I

L

Q

G

G

E

E

G

G

F

Y

Y

V

A

V

A

P

P

P

A

K

S

N

F

F

L

F

K

K

A

E

I

L

R

K

D

K

I

L

C

A

D

D

E

K

H

H

G

G

I

I

L

L

I

I

I

D

D

D

E

E

V

V

Q
|
Q

S

M

G

G

F

M

G

G

R

R

T

T

G

G

K

R

M

M

F

W

A

A

I

I

E

E

H

H

S

F

G

D

V

I

E

V

P

P

D

D

I

I

I

V

C

T

M

V

A

A

K
|
K

S

A

M

L

A

G

D

G

G

G

M

I

P

P

I

I

S

G

A

A

V

T

V

I

-

F

G

R

T

A

D

D

K

L

V

D

M

F

D

G

A

V

S

S

G

G

G

V

N

H

S

S

L

-

G

-

G

N

T
|
T

Y

F

T

G

G

G

S

N

P

T

V

V

S

A

C

A

A

A

T

A

L

L

A

A

V

V

L

I

E

E

V

E

F

L

E

-

E

Q

E

N

K

G

I

L

L

I

E

E

K

N

S

A

Q

Q

A

K

L

L

G

E

D

P

K

L

L

F

A

R

K

E

R

R

F

L

S

E

Q

E

W

M

E

K

Q

E

D

K

F

Y

D

E

C

I

V

I

D

G

N

D

G

V

R

R

N

G

L

L

G

G

A

L

M

A

A

W

A

G

F

V

D

E

L

F

V

V

S

K

D

D

K

R

A

K

Q

T

H

K

T

E

P

Y

D

A

A

T

D

K

L

E

A

R

G

G

A

E

L

I

C

V

K

V

R

E

A

A

R

L

E

K

K

R

G

G

L

L

V

A

L

L

S

G

C

C

G

G

L

-

Y

-

G

K

N

S

T

A

I

I

R

R

F

L

L

I

M

P

P

P

V

L

T

I

I

I

E

S

D

E

I

E

L

A

E

K

E

M

G

G

L

L

G

D

V

I

V

F

E

E

S

E

A

A

L

I

K

K

T92 (= T78) mutation to V: Slightly increases the specific activity. Increases KM for L-ornithine.
D93 (vs. gap) mutation to L: Reduces the specific activity. Increases KM for L-ornithine.
G124 (= G110) binding pyridoxal 5'-phosphate
T125 (≠ A111) binding pyridoxal 5'-phosphate
Q267 (= Q241) binding pyridoxal 5'-phosphate
K293 (= K267) modified: N6-(pyridoxal phosphate)lysine
T321 (= T296) binding pyridoxal 5'-phosphate

7vo1A Structure of aminotransferase-substrate complex (see paper)
37% identity, 96% coverage: 8:421/432 of query aligns to 18:441/452 of 7vo1A

query
sites
7vo1A

E

E

L

E

K

H

Q

H

R

K

Y

Y

V

M

A

A

N

T

G

T

A

T

A

N

S

D

P

P

A

N

T

E

Q

Y

F

F

-

L

-

V

A

I

D

E

R

R

A

A

E

E

N

G

A

V

L

Y

I

W

W

I

D

D

A

V

D

D

G

G

N

N

R

V

L

L

I

L

D

D

F

F

A

S

G

S

G

G

I
|
I

G

G

V

V

L

M

N

N

I

V

G

G

H

L

R

R

H

N

P

P

K

K

V

V

V

I

E

E

A

A

V

I

K

K

A

K

Q

Q

L

L

D

D

K

L

V

V

M

L

H

H

T

A

C

A

Q

G

T

T

-

D

-

Y

V

Y

M

N

P

P

Y

Y

E

Q

G

-

Y

-

V

V

K

E

V

L

A

A

E

K

K

K

L

L

S

V

Q

E

I

I

T

A

P

P

V

G

R

D

G

I

H

E

A

R

K

K

V

V

M

F

L

L

A

S

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

L

N

E

E

N

A

A

A

V

L

K

K

V

I

A

A

R

K

A

W

A

S

T

T

G

N

K

R

N

K

N

M

V

F

I

I

C

A

F

F

D

I

G

G

G

A

Y
x
F

H
|
H

G

G

R
|
R

T

T

F

H

M

G

T

T

M

M

A

S

M

L

N

T

-

A

G

S

K

K

V

P

A

V

P

Q

Y

R

A

S

T

R

D

M

F

F

G

P

S

T

M

M

P

P

G

G

N

-

V

V

F

V

R

H

A

V

P

P

Y

Y

P

P

V

N

P

P

Y

Y

H

R

-

N

-

P

-

W

G

G

V

I

-

D

-

G

-

Y

-

E

S

N

E

P

D

D

E

E

A

L

L

I

R

N

G

R

L

V

-

I

-

D

-

Y

-

I

-

E

K

E

M

Y

A

L

L

F

K

E

T

H

D

Y

A

V

N

P

P

A

K

E

D

E

T

V

A

A

A

G

I

I

V

F

L

F

E

E

P

P

V

I

L

Q

G

G

E
|
E

G

G

F

Y

Y

V

A

V

A

P

P

P

A

K

S

N

F

F

L

F

K

K

A

E

I

L

R

K

D

K

I

L

C

A

D

D

E

K

H

H

G

G

I

I

L

L

I

I

I

D

D
|
D

E

E

V
|
V

Q
|
Q

S

M

G

G

F

M

G

G

R

R

T

T

G

G

K

R

M

M

F

W

A

A

I

I

E

E

H

H

S

F

G

D

V

I

E

V

P

P

D

D

I

I

I

V

C

T

M

V

A

A

K
|
K

S

A

M

L

A

G

D

G

G

G

M

I

P

P

I

I

S

G

A

A

V

T

V

I

-

F

G

R

T

A

D

D

K

L

V

D

M

F

D

G

A

V

S

S

G

G

G

V

N

H

S

S

L

-

G

-

G
x
N

T
|
T

Y

F

T

G

G

G

S

N

P

T