SitesBLAST
Comparing WP_043528536.1 NCBI__GCF_000759345.1:WP_043528536.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
67% identity, 99% coverage: 12:1233/1233 of query aligns to 8:1227/1227 of P13009
- C247 (= C250) binding Zn(2+)
- C310 (= C313) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- C311 (= C314) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- E694 (= E696) binding methylcob(III)alamin
- GDVHD 756:760 (= GDVHD 759:763) binding methylcob(III)alamin
- D757 (= D760) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
- H759 (= H762) binding axial binding residue; mutation to G: Loss of catalytic activity.
- S804 (= S807) binding methylcob(III)alamin
- T808 (= T811) binding methylcob(III)alamin
- S810 (= S813) mutation to A: Decreases activity by about 40%.
- A860 (= A863) binding methylcob(III)alamin
- D946 (= D950) binding S-adenosyl-L-methionine
- R1134 (= R1140) binding S-adenosyl-L-methionine
- YY 1189:1190 (≠ YF 1195:1196) binding S-adenosyl-L-methionine
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
56% identity, 100% coverage: 3:1231/1233 of query aligns to 9:1263/1265 of Q99707
- R61 (≠ S51) natural variant: R -> K
- C255 (≠ S245) to Y: in dbSNP:rs1140598
- GSR 382:384 (≠ GSA 370:372) binding (6S)-5,6,7,8-tetrahydrofolate
- D449 (= D437) binding (6S)-5,6,7,8-tetrahydrofolate
- N470 (= N458) binding (6S)-5,6,7,8-tetrahydrofolate
- D537 (= D525) binding (6S)-5,6,7,8-tetrahydrofolate
- N579 (= N567) binding (6S)-5,6,7,8-tetrahydrofolate
- R585 (= R573) binding (6S)-5,6,7,8-tetrahydrofolate
- R591 (= R579) binding (6S)-5,6,7,8-tetrahydrofolate
- D919 (≠ R896) to G: in dbSNP:rs1805087
- D963 (= D939) mutation to E: Decreases binding to MTRR; when associated with N-1071.
- K1071 (= K1040) mutation to N: Decreases binding to MTRR; when associated with E-963.
3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
65% identity, 47% coverage: 655:1231/1233 of query aligns to 3:575/576 of 3ivaA
- active site: D107 (= D760), H109 (= H762), S160 (= S813)
- binding cobalamin: H109 (= H762), G112 (= G765), V116 (= V769), G152 (= G805), L153 (= L806), S154 (= S807), L156 (= L809), I157 (= I810), T158 (= T811), G183 (= G836), G184 (= G837), Q208 (≠ T861), N209 (≠ D862), T303 (≠ S957), D443 (= D1099), A486 (= A1142), G488 (= G1144), Y489 (= Y1145), H495 (= H1151), A520 (= A1176), M521 (= M1177), G524 (≠ A1180), V527 (= V1183), S528 (= S1184)
- binding s-adenosyl-l-homocysteine: E447 (= E1103), R484 (= R1140), P485 (= P1141), Y489 (= Y1145), A491 (= A1147), Y539 (= Y1195)
3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
65% identity, 47% coverage: 655:1231/1233 of query aligns to 3:575/577 of 3bulA
- active site: D107 (= D760), H109 (= H762), S160 (= S813)
- binding cobalamin: H109 (= H762), V116 (= V769), G152 (= G805), L153 (= L806), S154 (= S807), L156 (= L809), I157 (= I810), T158 (= T811), G183 (= G836), G184 (= G837), Q208 (≠ T861), N209 (≠ D862), A210 (= A863), T213 (≠ A866), M302 (= M956), D443 (= D1099), A486 (= A1142), P487 (= P1143), G488 (= G1144), Y489 (= Y1145), H495 (= H1151), K498 (= K1154), M521 (= M1177), G524 (≠ A1180), V527 (= V1183), S528 (= S1184)
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
55% identity, 51% coverage: 11:638/1233 of query aligns to 1:610/611 of 4cczA
- binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E364), G342 (= G370), R344 (≠ A372), N430 (= N458), M458 (= M486), D497 (= D525), G536 (= G564), S538 (= S566), N539 (= N567), F542 (= F570), R545 (= R573), R551 (= R579)
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
37% identity, 67% coverage: 15:845/1233 of query aligns to 8:806/841 of 8g3hA