SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043529620.1 NCBI__GCF_000759345.1:WP_043529620.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
63% identity, 98% coverage: 6:401/404 of query aligns to 9:404/405 of P40732

query
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P40732

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GT 108:109 (≠ GG 105:106) binding pyridoxal 5'-phosphate
K255 (= K252) modified: N6-(pyridoxal phosphate)lysine
T284 (= T281) binding pyridoxal 5'-phosphate

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
62% identity, 98% coverage: 6:401/404 of query aligns to 4:399/402 of 4jevB

query
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4jevB

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active site: F136 (= F138), E188 (= E190), D221 (= D223), Q224 (= Q226), K250 (= K252), T279 (= T281), R372 (= R374)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (= I48), S102 (= S104), G103 (= G105), T104 (≠ G106), F136 (= F138), H137 (= H139), E188 (= E190), E193 (= E195), D221 (= D223), V223 (= V225), Q224 (= Q226), K250 (= K252), R372 (= R374)

4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
60% identity, 99% coverage: 6:403/404 of query aligns to 4:401/401 of 4adbB

query
sites
4adbB

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active site: F136 (= F138), E188 (= E190), D221 (= D223), Q224 (= Q226), K250 (= K252), T279 (= T281), R372 (= R374)
binding pyridoxal-5'-phosphate: S102 (= S104), G103 (= G105), A104 (≠ G106), F136 (= F138), H137 (= H139), D221 (= D223), V223 (= V225), Q224 (= Q226), K250 (= K252)

4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
61% identity, 98% coverage: 6:401/404 of query aligns to 4:399/400 of 4addA

query
sites
4addA

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T

K

L

F

W

W

H

H

L

T

S

G

N

N

V

G

Y

Y

T

T

N

N

E

E

P

P

A

V

L

L

K

R

L

L

A

A

K

K

S

K

L

L

V

I

E

D

R

A

T

T

F

F

A

A

D

D

K

R

V

V

F

F

L

F

C

C

S

N

S

S

G
|
G

G
x
A

E

E

A

A

N

N

E

E

A

A

L

L

K

K

L

L

A

A

R

R

R

K

Y

F

I

A

Y

H

E

D

K

R

H

Y

G

G

E

S

Q

H

K

K

D

S

K

G

I

I

I

V

S

A

F

F

R

K

Q

N

S

A

F
|
F

H
|
H

G

G

R
|
R

T

T

F

L

F

F

T

T

V

V

S

S

V

A

G

G

Q

Q

P

P

K

A

Y

Y

S

S

Q

Q

G

D

F

F

G

A

P

P

V

L

P

P

G

A

G

D

I

I

Q

R

H

H

A

A

E

A

Y

Y

N

N

D

D

L

I

D

N

S

S

V

A

R

S

A

A

L

L

I

I

D

D

N

S

T

T

C

C

A

A

I

V

M

I

V

V

E
|
E

P

P

M

I

Q

Q

G

G

E

E

G

G

I

V

V

V

P

P

A

A

T

S

Q

N

E

A

F

F

L

L

S

Q

G

G

L

L

R

R

E

E

L

L

C

C

D

N

Q

R

H

H

Q

N

A

A

L

L

I

I

F

F

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

V

V

G

G

R

R

S

T

G

G

K

E

F

L

F

Y

A

A

Y

Y

M

M

H

H

Y

Y

G

G

V

V

T

T

P

P

D

D

I

L

L

L

T

T

S

T

A

A

K
|
K

S

A

L

L

G

G

F

F

P

P

I

V

G

G

A

A

M

L

L

L

T

A

T

T

D

E

K

E

I

C

A

A

P

R

S

V

L

M

A

T

I

V

G

G

T

T

H

H

G

G

S

T

T
|
T

Y

Y

G

G

N

N

A

P

L

L

A

A

S

S

A

A

V

V

A

A

L

G

A

K

A

V

V

L

E

E

H

L

I

I

D

N

T

T

P

P

E

E

V

M

L

L

E

N

G

G

V

V

Q

K

K

Q

R

R

H

H

D

D

L

W

F

F

R

V

E

E

H

R

L

L

E

N

A

T

I

I

N

N

E

H

K

R

H

Y

G

G

V

L

F

F

K

S

E

E

I

V

R

R

G

G

M

L

G

G

L

L

V

I

G

G

A

C

Q

V

M

L

S

N

D

A

A

D

Y

Y

E

A

G

G

R

Q

A

A

K

K

D

Q

I

I

L

S

P

Q

L

E

A

A

I

A

E

K

E

A

G

G

V

V

M

M

A

V

L

L

I

I

A

A

G

G

P

G

N

N

V

V

L

V

R
|
R

L

F

A

A

P

P

S

A

L

L

V

N

I

V

P

S

E

E

S

E

D

E

I

V

D

T

E

T

G

G

M

L

T

D

R

R

L

F

A

A

R

A

A

A

I

C

D

E

R

H

L

F

V

V

active site: F136 (= F138), E188 (= E190), D221 (= D223), Q224 (= Q226), K250 (= K252), T279 (= T281), R372 (= R374)
binding pyridoxal-5'-phosphate: G103 (= G105), A104 (≠ G106), F136 (= F138), H137 (= H139), D221 (= D223), V223 (= V225), K250 (= K252)
binding n~2~-(3-carboxypropanoyl)-l-ornithine: Y16 (= Y18), F136 (= F138), R139 (= R141)

4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
62% identity, 98% coverage: 6:401/404 of query aligns to 4:394/397 of 4jewA

query
sites
4jewA

T

T

R

R

S

A

D

T

F

F

D

D

Q

E

Y

V

M

I

A

L

P

P

N

V

Y

Y

S

A

P

P

Q

A

Q

D

V

F

I

I

P

P

V

V

R
x
K

G

G

K
|
K

G

G

S

S

R

R

L

V

W
|
W

D

D

Q

Q

E

Q

G

G

R

K

E

E

Y

Y

I

I

D

D

F

F

A

A

G

G

I

I

A

A

V

V

N

T

A

A

L

L

G

G

H

H

C

C

H

H

P

P

V

A

L

L

V

V

E

E

A

A

L

L

K

K

T

S

Q

Q

G

G

E

E

T

T

L

L

W

W

H

H

L

T

S

S

N

N

V

V

Y

F

T

T

N

N

E

E

P

P

A

A

L

L

K

R

L

L

A

G

K

R

S

K

L

L

V

I

E

D

R

A

T

T

F

F

A

A

D

E

K

R

V

V

F

L

L

F

C

M

S

N

S

S

G
|
G

G
x
T

E

E

A

A

N

N

E

E

A

T

A

A

L

F

K

K

L

L

A

A

R

R

R

H

Y

Y

I

A

Y

C

E

V

K

R

H

H

G

S

E

P

Q

F

K

K

D

T

K

K

I

I

S

A

F

F

R

H

Q

N

S

A

F
|
F

H
|
H

G

G

R
|
R

T

S

F

L

F

F

T

T

V

V

S

S

V

V

G

G

Q

Q

P

P

K

K

Y

Y

S

S

Q

D

G

G

F

F

G

G

P

P

V

K

P

P

G

A

G

D

I

I

Q

I

H

H

A

V

E

P

Y

F

N

N

D

D

L

L

D

H

S

A

V

V

R

K

A

A

L

V

I

M

D

D

N

H

T

T

C

C

A

A

I

V

M

V

V

V

E
|
E

P

P

M

I

Q

Q

G

G

E
|
E

G

G

I

V

V

Q

P

A

A

A

T

T

Q

P

E

E

F

F

L

L

S

K

G

G

L

L

R

R

E

D

L

L

C

C

D

D

Q

E

H

H

Q

Q

A

A

L

L

I

V

F

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

V

M

G

G

R

R

S

T

G

G

K

D

F

L

F

F

A

A

Y

Y

M

M

H

H

Y

Y

G

G

V

V

T

T

P

P

D

D

I

I

L

L

T

T

S

S

A

A

K
|
K

S

A

L

L

G

G

F

F

P

P

I

V

G

S

A

A

M

M

L

L

T

T

D

Q

K

E

I

I

A

A

P

S

S

A

L

F

A

-

I

-

G

-

T

-

H

-

G

G

S

S

T
|
T

Y

Y

G

G

N

N

A

P

L

L

A

A

S

C

A

A

V

V

A

A

L

G

A

A

A

T

V

F

E

D

H

I

I

I

D

N

T

T

P

P

E

E

V

V

L

L

E

Q

G

G

V

I

Q

H

K

T

R

K

H

R

D

Q

L

Q

F

F

R

V

E

Q

H

H

L

L

E

Q

A

A

I

I

N

D

E

E

K

Q

H

F

G

D

V

I

F

F

K

S

E

D

I

I

R

R

G

G

M

M

G

G

L

L

V

I

G

G

A

A

Q

E

M

L

S

K

D

P

A

K

Y

Y

E

K

G

G

R

R

A

A

K

R

D

D

I

F

L

L

P

Y

L

A

A

G

I

A

E

E

E

A

G

G

V

V

M

M

A

V

L

L

I

N

A

A

G

G

P

A

N

D

V

V

L

M

R
|
R

L

F

A

A

P

P

S

S

L

L

V

V

I

V

P

E

E

E

S

A

D

D

I

I

D

H

E

E

G

G

M

M

T

Q

R

R

L

F

A

A

R

Q

A

A

I

V

D

G

R

K

L

V

V

V

active site: F136 (= F138), E188 (= E190), D221 (= D223), Q224 (= Q226), K250 (= K252), T274 (= T281), R367 (= R374)
binding (2S)-2-azanyl-4-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxy-butanoic acid: G103 (= G105), T104 (≠ G106), F136 (= F138), H137 (= H139), R139 (= R141), E188 (= E190), E193 (= E195), D221 (= D223), V223 (= V225), K250 (= K252)
binding picric acid: K25 (≠ R27), K27 (= K29), W32 (= W34)

2pb0A Structure of biosynthetic n-acetylornithine aminotransferase from salmonella typhimurium: studies on substrate specificity and inhibitor binding (see paper)
62% identity, 97% coverage: 10:401/404 of query aligns to 2:388/389 of 2pb0A

query
sites
2pb0A

F

F

D

D

Q

E

Y

V

M

I

A

L

P

P

N

V

Y

Y

S

A

P

P

Q

A

Q

D

V

F

I

I

P

P

V

V

R

K

G

G

K

K

G

G

S

S

R

R

L

V

W

W

D

D

Q

Q

E

Q

G

G

R

K

E

E

Y

Y

I

I

D

D

F

F

A

A

G

G

I

I

A

A

V

V

N

T

A

A

L

L

G

G

H

H

C

C

H

H

P

P

V

A

L

L

V

V

E

E

A

A

L

L

K

K

T

S

Q

Q

G

G

E

E

T

T

L

L

W

W

H

H

L

T

S

S

N

N

V

V

Y

F

T

T

N

N

E

E

P

P

A

A

L

L

K

R

L

L

A

G

K

R

S

K

L

L

V

I

E

D

R

A

T

T

F

F

A

A

D

E

K

R

V

V

F

L

L

F

C

M

S

N

S
|
S

G
|
G

G
x
T

E

E

A

A

N

N

E

E

A

T

A

A

L

F

K

K

L

L

A

A

R

R

R

H

Y

Y

I

A

Y

C

E

V

K

R

H

H

G

S

E

P

Q

F

K

K

D

T

K

K

I

I

S

A

F

F

R

H

Q

N

S

A

F
|
F

H
|
H

G

G

R

R

T

S

F

L

F

F

T

T

V

V

S

S

V

V

G

G

Q

Q

P

P

K

K

Y

Y

S

S

Q

D

G

G

F

F

G

G

P

P

V

K

P

P

G

A

G

D

I

I

Q

I

H

H

A

V

E

P

Y

F

N

N

D

D

L

L

D

H

S

A

V

V

R

K

A

A

L

V

I

M

D

D

N

H

T

T

C

C

A

A

I

V

M

V

V

V

E
|
E

P

P

M

I

Q

Q

G

G

E

E

G

G

I

V

V

Q

P

A

A

A

T

T

Q

P

E

E

F

F

L

L

S

K

G

G

L

L

R

R

E

D

L

L

C

C

D

D

Q

E

H

H

Q

Q

A

A

L

L

I

V

F

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

V

M

G

G

R

R

S

T

G

G

K

D

F

L

F

F

A

A

Y

Y

M

M

H

H

Y

Y

G

G

V

V

T

T

P

P

D

D

I

I

L

L

T

T

S

S

A

A

K
|
K

S

A

L

L

G

G

F

F

P

P

I

V

G

S

A

A

M

M

L

L

T

T

D

Q

K

E

I

I

A

A

P

S

S

A

L

F

A

-

I

-

G

-

T

-

H

-

G

G

S

S

T
|
T

Y

Y

G

G

N

N

A

P

L

L

A

A

S

C

A

A

V

V

A

A

L

G

A

A

V

F

E

D

H

I

I

I

D

N

T

T

P

P

E

E

V

V

L

L

E

Q

G

G

V

I

Q

H

K

T

R

K

H

R

D

Q

L

Q

F

F

R

V

E

Q

H

H

L

L

E

Q

A

A

I

I

N

D

E

E

K

Q

H

F

G

D

V

I

F

F

K

S

E

D

I

I

R

R

G

G

M

M

G

G

L

L

V

I

G

G

A

A

Q

E

M

L

S

K

D

P

A

K

Y

Y

E

K

G

G

R

R

A

A

K

R

D

D

I

F

L

L

P

Y

L

A

A

G

I

A

E

E

E

A

G

G

V

V

M

M

A

V

L

L

I

N

A

A

G

G

P

A

N

D

V

V

L

M

R
|
R

L

F

A

A

P

P

S

S

L

L

V

V

I

V

P

E

E

E

S

A

D

D

I

I

D

H

E

E

G

G

M

M

T

Q

R

R

L

F

A

A

R

Q

A

A

I

V

D

G

R

K

L

V

V

V

active site: F130 (= F138), E182 (= E190), D215 (= D223), Q218 (= Q226), K244 (= K252), T268 (= T281), R361 (= R374)
binding pyridoxal-5'-phosphate: S96 (= S104), G97 (= G105), T98 (≠ G106), F130 (= F138), H131 (= H139), E182 (= E190), D215 (= D223), V217 (= V225), Q218 (= Q226), K244 (= K252)

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
44% identity, 98% coverage: 8:401/404 of query aligns to 29:429/429 of P73133

query
sites
P73133

S

A

D

D

F

F

D

D

Q

T

Y

Y

M

V

A

M

P

N

N

T

Y
|
Y

S

G

P

R

Q

F

Q

P

V

I

I

A

P

I

V

A

R

R

G

G

K

Q

G

G

S

S

R

T

L

L

W

W

D

D

Q

T

E

E

G

G

R

K

E

S

Y

Y

I

L

D

D

F

F

A

V

G

A

G

G

I

I

A

A

V

T

N

C

A

T

L

L

G

G

H

H

C

A

H

H

P

P

V

A

L

L

V

V

E

R

A

A

L

V

K

S

T

D

Q

Q

G

I

E

Q

T

K

L

L

W

H

H

H

L

V

S

S

N

N

V

L

Y

Y

T

Y

N

I

E

P

P

E

A

Q

L

G

K

E

L

L

A

A

K

K

S

W

L

I

V

V

E

E

R

H

T

S

F

C

A

A

D

D

K

R

V

V

F

F

L

F

C

C

S

N

S
|
S

G
|
G

G
x
A

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

L

A

V

R

R

R

K

Y

Y

I

A

Y

H

E

T

K

V

-

L

H

D

G

F

E

L

Q

E

K

Q

D

P

K

V

I

I

I

L

S

T

F

A

R

K

Q

A

S

S

F

F

H

H

G

G

R
|
R

T

T

F

L

F

A

T

T

V

I

S

T

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

Y

S

Q

Q

Q

G

Y

F

F

G

D

P

P

V

L

P

V

G

P

G

G

I

F

Q

D

H

Y

A

V

E

P

Y

Y

N

N

D

D

L

I

D

R

S

S

V

L

R

E

A

N

L

K

I

V

D

A

D

D

-

L

-

D

-

E

-

G

-

N

-

S

N

R

T

V

C

A

A

A

I

I

M

F

V

L

E

E

P

P

M

L

Q

Q

G

G

E
|
E

G

G

I

V

V

R

P

P

A

G

T

D

Q

L

E

A

F

Y

L

F

S

K

G

R

L

V

R

R

E

E

L

I

C

C

D

D

Q

Q

H

N

Q

D

A

I

L

L

I

V

F

F

D
|
D

E

E

V

V

Q
|
Q

T

V

G

G

V

V

G

G

R

R

S

T

G

G

K

K

F

L

F

W

A

G

Y

Y

M

E

H

H

Y

L

G

G

V

V

T

E

P

P

D

D

I

I

L

F

T

T

S

S

A

A

K
|
K

S

G

L

L

G

A

G

G

F

V

P

P

I

I

G

G

A

A

M

M

L

M

T

-

T

C

D

K

K

K

I

F

A

C

P

D

S

V

L

F

A

E

I

P

G

G

T

N

H

H

G

A

S

S

T
|
T

Y

F

G

G

N

N

A

P

L

L

A

A

S

C

A

A

V

A

A

G

L

L

A

A

A

V

V

L

E

K

H

T

I

I

D

E

T

G

P

D

E

R

V

L

L

L

E

D

G

N

V

V

Q

Q

K

A

R

R

H

G

D

E

L

Q

F

L

R

R

E

S

H

G

L

L

E

A

A

E

I

I

-

K

N

N

E

Q

K

Y

H

P

G

T

V

L

F

F

K

T

E

E

I

V

R

R

G

G

M

W

G

G

L

L

L

I

V

N

G

G

A

L

Q

E

M

I

S

S

D

A

A

E

Y

S

E

S

G

L

R

T

A

S

K

V

D

E

I

I

L

V

P

K

L

A

A

A

I

M

E

E

E

Q

G

G

V

L

M

L

A

L

L

A

I

P

A

A

G

G

P

P

N

K

V

V

L

L

R
|
R

L

F

A

V

P

P

S

P

L

L

V

V

I

V

P

T

E

E

S

A

D

E

I

I

D

A

E

Q

G

A

M

V

T

E

R

I

L

L

A

R

R

Q

A

A

I

I

D

A

R

T

L

L

V

V

Y39 (= Y18) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
S125 (= S104) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G105) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (≠ G106) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (= R141) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (= E195) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D223) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q226) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K252) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T281) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (= R374) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
42% identity, 95% coverage: 13:397/404 of query aligns to 2:382/385 of Q9X2A5

query
sites
Q9X2A5

Y

Y

M

L

A

M

P

N

N

T

Y

Y

S

S

P

R

Q

F

Q

P

V

A

I

T

P

F

V

V

R

Y

G

G

K

K

G

G

S

S

R

W

L

I

W

Y

D

D

Q

E

E

K

G

G

R

N

E

A

Y

Y

I

L

D

D

F

F

A

T

G

S

G

G

I

I

A

A

V

V

N

N

A

V

L

L

G

G

H

H

C

S

H

H

P

P

V

R

L

L

V

V

E

E

A

A

L

I

K

K

T

D

Q

Q

G

A

E

E

T

K

L

L

W

I

H

H

L

C

S

S

N

N

V

L

Y

F

T

W

N

N

E

R

P

P

A

Q

L

M

K

E

L

L

A

A

K

E

S

L

L

L

V

S

E

K

R

N

T

T

F

F

A

G

D

G

K

K

V

V

F

F

L

F

C

A

S

N

S

T

G
|
G

G
x
T

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

I

A

A

R

R

R

K

Y

Y

I

G

Y

K

E

K

K

K

H

S

G

E

E

K

Q

K

K

Y

D

-

K

R

I

I

I

L

S

S

F

A

R

H

Q

N

S

S

F

F

H

H

G

G

R

R

T

T

F

L

F

G

T

S

V

L

S

T

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

Y

S

Q

Q

K

G

P

F

F

G

E

P

P

V

L

P

V

G

P

G

G

I

F

Q

E

H

Y

A

F

E

E

Y

F

N

N

D

N

L

V

D

E

S

D

V

L

R

R

A

R

L

K

I

M

D

S

D

E

N

D

T

V

C

C

A

A

I

V

M

F

V

L

E

E

P

P

M

I

Q

Q

G

G

E

E

G

S

G

G

I

I

V

V

P

P

A

A

T

T

Q

K

E

E

F

F

L

L

S

E

G

E

L

A

R

R

E

K

L

L

C

C

D

D

Q

E

H

Y

Q

D

A

A

L

L

I

V

F

F

D

D

E

E

V

V

Q

Q

T

C

G

G

V

M

G

G

R

R

S

T

G

G

K

K

F

L

F

F

A

A

Y

Y

M

Q

H

K

Y

Y

G

G

V

V

T

V

P

P

D

D

I

V

L

L

T

T

S

T

A

A

K

K

S

G

L

L

G

G

F

V

P

P

I

I

G

G

A

A

M

V

L

I

T

V

T

N

D

E

K

R

I

-

A

A

P

N

S

V

L

L

A

E

I

P

G

G

T

D

H

H

G

G

S

T

T
|
T

Y

F

G

G

N

N

A

P

L

L

A

A

S

C

A

R

V

A

A

G

L

V

A

T

A

V

V

I

E

K

H

E

I

L

D

T

T

K

P

E

E

G

V

F

L

L

E

E

G

E

V

V

Q

E

K

E

R

K

H

G

D

N

L

Y

F

L

R

M

E

K

H

K

L

L

E

Q

A

E

I

M

N

K

E

E

K

E

H

Y

G

D

V

V

F

V

K

A

E

D

I

V

R

R

G

G

M

M

G

G

L

L

L

M

V

I

G

G

A

I

Q

Q

M

F

S

R

D

E

A

E

Y

V

E

S

G

N

R

R

A

-

K

-

D

E

I

V

L

A

P

T

L

K

A

C

I

F

E

E

E

N

G

K

V

L

M

L

A

V

L

V

I

P

A

A

G

G

P

N

N

N

V

T

L

I

R

R

L

F

A

L

P

P

S

P

L

L

V

T

I

V

P

E

E

Y

S

G

D

E

I

I

D

D

E

L

G

A

M

V

T

E

R

T

L

L

A

K

R

K

A

V

I

L

GT 94:95 (≠ GG 105:106) binding pyridoxal 5'-phosphate
T268 (= T281) binding pyridoxal 5'-phosphate

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
42% identity, 95% coverage: 13:397/404 of query aligns to 10:390/393 of 2ordA

query
sites
2ordA

Y

Y

M

L

A

M

P

N

N

T

Y

Y

S

S

P

R

Q

F

Q

P

V

A

I

T

P

F

V

V

R

Y

G

G

K

K

G

G

S

S

R

W

L

I

W

Y

D

D

Q

E

E

K

G

G

R

N

E

A

Y

Y

I

L

D

D

F

F

A

T

G

S

G

G

I

I

A

A

V

V

N

N

A

V

L

L

G

G

H

H

C

S

H

H

P

P

V

R

L

L

V

V

E

E

A

A

L

I

K

K

T

D

Q

Q

G

A

E

E

T

K

L

L

W

I

H

H

L

C

S

S

N

N

V

L

Y

F

T

W

N

N

E

R

P

P

A

Q

L

M

K

E

L

L

A

A

K

E

S

L

L

L

V

S

E

K

R

N

T

T

F

F

A

G

D

G

K

K

V

V

F

F

L

F

C

A

S

N

S

T

G
|
G

G
x
T

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

I

A

A

R

R

R

K

Y

Y

I

G

Y

K

E

K

K

K

H

S

G

E

E

K

Q

K

K

Y

D

-

K

R

I

I

I

L

S

S

F

A

R

H

Q

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

F

L

F

G

T

S

V

L

S

T

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

Y

S

Q

Q

K

G

P

F

F

G

E

P

P

V

L

P

V

G

P

G

G

I

F

Q

E

H

Y

A

F

E

E

Y

F

N

N

D

N

L

V

D

E

S

D

V

L

R

R

A

R

L

K

I

M

D

S

D

E

N

D

T

V

C

C

A

A

I

V

M

F

V

L

E
|
E

P

P

M

I

Q

Q

G

G

E

E

G

S

G

G

I

I

V

V

P

P

A

A

T

T

Q

K

E

E

F

F

L

L

S

E

G

E

L

A

R

R

E

K

L

L

C

C

D

D

Q

E

H

Y

Q

D

A

A

L

L

I

V

F

F

D
|
D

E

E

V
|
V

Q
|
Q

T

C

G

G

V

M

G

G

R

R

S

T

G

G

K

K

F

L

F

F

A

A

Y

Y

M

Q

H

K

Y

Y

G

G

V

V

T

V

P

P

D

D

I

V

L

L

T

T

S

T

A

A

K
|
K

S

G

L

L

G

G

F

V

P

P

I

I

G

G

A

A

M

V

L

I

T

V

T

N

D

E

K

R

I

-

A

A

P

N

S

V

L

L

A

E

I

P

G

G

T

D

H

H

G

G

S

T

T
|
T

Y

F

G

G

N

N

A

P

L

L

A

A

S

C

A

R

V

A

A

G

L

V

A

T

A

V

V

I

E

K

H

E

I

L

D

T

T

K

P

E

E

G

V

F

L

L

E

E

G

E

V

V

Q

E

K

E

R

K

H

G

D

N

L

Y

F

L

R

M

E

K

H

K

L

L

E

Q

A

E

I

M

N

K

E

E

K

E

H

Y

G

D

V

V

F

V

K

A

E

D

I

V

R

R

G

G

M

M

G

G

L

L

L

M

V

I

G

G

A

I

Q

Q

M

F

S

R

D

E

A

E

Y

V

E

S

G

N

R

R

A

-

K

-

D

E

I

V

L

A

P

T

L

K

A

C

I

F

E

E

E

N

G

K

V

L

M

L

A

V

L

V

I

P

A

A

G

G

P

N

N

N

V

T

L

I

R
|
R

L

F

A

L

P

P

S

P

L

L

V

T

I

V

P

E

E

Y

S

G

D

E

I

I

D

D

E

L

G

A

M

V

T

E

R

T

L

L

A

K

R

K

A

V

I

L

active site: F134 (= F138), E186 (= E190), D219 (= D223), Q222 (= Q226), K248 (= K252), T276 (= T281), R367 (= R374)
binding pyridoxal-5'-phosphate: G102 (= G105), T103 (≠ G106), F134 (= F138), H135 (= H139), E186 (= E190), D219 (= D223), V221 (= V225), Q222 (= Q226), K248 (= K252)

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
44% identity, 94% coverage: 13:393/404 of query aligns to 3:372/376 of O66442

query
sites
O66442

Y

Y

M

L

A

M

P

N

N

N

Y

Y

S

A

P

R

Q

L

Q

P

V

V

I

K

P

F

V

V

R

R

G

G

K

K

G

G

S

V

R

Y

L

L

W

Y

D

D

Q

E

E

E

G

G

R

K

E

E

Y

Y

I

L

D

D

F

F

A

V

G

S

G

G

I

I

A

G

V

V

N

N

A

S

L

L

G

G

H

H

C

A

H

Y

P

P

V

K

L

L

V

T

E

E

A

A

L

L

K

K

T

E

Q

Q

G

V

E

E

T

K

L

L

W

L

H

H

L

V

S

S

N

N

V

L

Y

Y

T

E

N

N

E

P

P

W

A

Q

L

E

K

E

L

L

A

A

K

H

S

K

L

L

V

V

E

K

R

H

T

F

F

W

A

T

D

E

-

G

K

K

V

V

F

F

L

F

C

A

S

N

S

S

G
|
G

G
x
T

E

E

A

S

N

V

E

E

A

A

L

I

K

K

L

L

A

A

R

R

R

K

Y

Y

I

W

Y

R

E

D

K

K

H

-

G

G

E

K

Q

N

K

K

D

W

K

K

I

F

I

I

S

S

F

F

R

E

Q

N

S

S

F

F

H

H

G

G

R

R

T

T

F

Y

F

G

T

S

V

L

S

S

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

F

S

H

Q

K

G

G

F

F

G

E

P

P

V

L

P

V

G

P

G

G

I

F

Q

S

H

Y

A

A

E

K

Y

L

N

N

D

D

L

I

D

D

S

S

V

V

R

Y

A

K

L

L

I

L

D

D

D

E

N

E

T

T

C

A

A

G

I

I

M

I

V

I

E

E

P

V

M

I

Q

Q

G

G

E

E

G

G

I

V

V

N

P

E

A

A

T

S

Q

E

E

D

F

F

L

L

S

S

G

K

L

L

R

Q

E

E

L

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C

C

D

K

Q

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H

K

Q

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A

V

L

L

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I

F

I

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D

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V

V

Q

Q

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T

G

G

V

I

G

G

R

R

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T

G

G

K

E

F

F

F

Y

A

A

Y

Y

M

Q

H

H

Y

F

G

N

V

L

T

K

P

P

D

D

I

V

L

I

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A

S

L

A

A

K
|
K

S

G

L

L

G

G

F

V

P

P

I

I

G

G

A

A

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I

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L

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A

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K

E

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V

A

A

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S

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A

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G

G

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H

H

G

G

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S

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|
T

Y

F

G

G

N

N

A

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L

L

A

A

S

C

A

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V

A

A

G

L

T

A

V

V

V

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D

H

E

I

V

D

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-

P

-

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L

L

L

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V

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N

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F

F

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E

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H

K

L

L

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K

A

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I

L

N

G

E

K

K

-

H

-

G

-

V

-

F

-

K

G

E

K

I

V

R

K

G

G

M

R

G

G

L

L

L

M

V

L

G

G

A

L

Q

E

M

L

S

-

D

-

A

-

Y

-

E

E

G

R

R

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A

C

K

K

D

D

I

Y

L

V

P

L

L

K

A

A

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L

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E

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G

G

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L

M

L

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N

I

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A

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A

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G

N

K

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L

L

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A

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K

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I

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A

M

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L

L

GT 96:97 (≠ GG 105:106) binding pyridoxal 5'-phosphate
K242 (= K252) modified: N6-(pyridoxal phosphate)lysine
T271 (= T281) binding pyridoxal 5'-phosphate

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
44% identity, 94% coverage: 13:393/404 of query aligns to 2:371/375 of 2eh6A

query
sites
2eh6A

Y

Y

M

L

A

M

P

N

N

N

Y

Y

S

A

P

R

Q

L

Q

P

V

V

I

K

P

F

V

V

R

R

G

G

K

K

G

G

S

V

R

Y

L

L

W

Y

D

D

Q

E

E

E

G

G

R

K

E

E

Y

Y

I

L

D

D

F

F

A

V

G

S

G

G

I

I

A

G

V

V

N

N

A

S

L

L

G

G

H

H

C

A

H

Y

P

P

V

K

L

L

V

T

E

E

A

A

L

L

K

K

T

E

Q

Q

G

V

E

E

T

K

L

L

W

L

H

H

L

V

S

S

N

N

V

L

Y

Y

T

E

N

N

E

P

P

W

A

Q

L

E

K

E

L

L

A

A

K

H

S

K

L

L

V

V

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K

R

H

T

F

F

W

A

T

D

E

-

G

K

K

V

V

F

F

L

F

C

A

S

N

S

S

G
|
G

G
x
T

E

E

A

S

N

V

E

E

A

A

L

I

K

K

L

L

A

A

R

R

R

K

Y

Y

I

W

Y

R

E

D

K

K

H

-

G

G

E

K

Q

N

K

K

D

W

K

K

I

F

I

I

S

S

F

F

R

E

Q

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

F

Y

F

G

T

S

V

L

S

S

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

F

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G

G

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F

G

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L

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V

G

P

G

G

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Y

A

A

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K

Y

L

N

N

D

D

L

I

D

D

S

S

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V

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A

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L

L

I

L

D

D

D

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N

E

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T

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A

A

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I

I

M

I

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I

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|
E

P

V

M

I

Q

Q

G

G

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E

G

G

I

V

V

N

P

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A

A

T

S

Q

E

E

D

F

F

L

L

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S

G

K

L

L

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L

I

C

C

D

K

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H

K

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A

V

L

L

I

I

F

I

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

V

I

G

G

R

R

S

T

G

G

K

E

F

F

F

Y

A

A

Y

Y

M

Q

H

H

Y

F

G

N

V

L

T

K

P

P

D

D

I

V

L

I

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A

S

L

A

A

K
|
K

S

G

L

L

G

G

F

V

P

P

I

I

G

G

A

A

M

I

L

L

T

A

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D

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K

E

I

V

A

A

P

Q

S

S

L

F

A

T

I

P

G

G

T

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H

H

G

G

S

S

T
|
T

Y

F

G

G

N

N

A

P

L

L

A

A

S

C

A

R

V

A

A

G

L

T

A

V

V

V

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D

H

E

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V

D

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P

-

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L

L

L

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N

L

Y

F

F

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K

L

L

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K

A

E

I

L

N

G

E

K

K

-

H

-

G

-

V

-

F

-

K

G

E

K

I

V

R

K

G

G

M

R

G

G

L

L

L

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L

G

G

A

L

Q

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M

L

S

-

D

-

A

-

Y

-

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R

R

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A

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K

K

D

D

I

Y

L

V

P

L

L

K

A

A

I

L

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K

G

G

V

L

M

L

A

I

L

N

I

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A

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A

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G

N

K

V

V

L

L

R
|
R

L

F

A

L

P

P

S

P

L

L

V

I

I

I

P

Q

E

K

S

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D

H

I

I

D

D

E

R

G

A

M

I

T

S

R

V

L

L

active site: F127 (= F138), E179 (= E190), D212 (= D223), Q215 (= Q226), K241 (= K252), T270 (= T281), R352 (= R374)
binding pyridoxal-5'-phosphate: G95 (= G105), T96 (≠ G106), F127 (= F138), H128 (= H139), E179 (= E190), D212 (= D223), V214 (= V225), K241 (= K252)

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
41% identity, 95% coverage: 17:400/404 of query aligns to 72:456/457 of Q9M8M7

query
sites
Q9M8M7

N

T

Y

Y

S

A

P

R

Q

A

Q

P

V

V

I

V

P

L

V

S

R

S

G

G

K

K

G

G

S

C

R

K

L

L

W

F

D

D

Q

P

E

E

G

G

R

K

E

E

Y

Y

I

L

D

D

F

C

A

A

G

S

G

G

I

I

A

A

V

V

N

N

A

A

L

L

G

G

H

H

C

G

H

D

P

P

V

D

L

W

V

L

E

R

A

A

L

V

K

T

T

E

Q

Q

G

A

E

G

T

V

L

L

W

A

H

H

L

V

S

S

N

N

V

V

Y

Y

T

Y

N

T

E

I

P

P

A

Q

L

I

K

E

L

L

A

A

K

K

S

R

L

L

V

V

E

A

R

S

T

S

F

F

A

A

D

D

K

R

V

V

F

F

L

F

C

C

S

N

S

S

G

G

G

T

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

F

A

S

R

R

R

K

Y

F

I

Q

Y

R

E

F

K

T

H

H

G

P

E

E

Q

D

K

K

D

E

-

V

-

A

-

T

K

G

I

F

I

I

S

A

F

F

R

T

Q

N

S

S

F

F

H

H

G

G

R

R

T

T

F

L

F

G

T

A

V

L

S

A

V

L

G

T

G

S

Q

K

P

E

K

Q

Y

Y

S

R

Q

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G

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F

F

G

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P

P

V

I

P

M

G

P

G

G

I

V

Q

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H

F

A

L

E

E

Y

Y

N

G

D

N

L

I

D

Q

S

A

V

A

R

T

A

D

L

L

I

I

D

R

D

S

-

G

N

K

T

I

C

A

A

A

I

V

M

F

V

V

E

E

P

P

M

I

Q

Q

G

G

E

E

G

G