SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043529788.1 NCBI__GCF_000759345.1:WP_043529788.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
37% identity, 98% coverage: 2:252/256 of query aligns to 3:253/260 of P02911

query
sites
P02911

K

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D33 (= D32) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
Y36 (= Y35) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
D52 (= D51) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
C60 (= C59) modified: Disulfide link with 67
C67 (= C66) modified: Disulfide link with 60
F74 (≠ W73) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
S91 (= S90) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
S92 (= S91) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
S94 (≠ T93) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
R99 (= R98) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
L139 (≠ Q139) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
T143 (≠ L143) binding L-arginine; binding L-lysine; binding L-ornithine
D183 (= D182) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
38% identity, 89% coverage: 25:252/256 of query aligns to 1:228/235 of 5owfA

query
sites
5owfA

E

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binding glutamine: Y11 (= Y35), F49 (≠ W73), S66 (= S90), S67 (= S91), S69 (≠ T93), R74 (= R98), K114 (≠ Q139), S117 (≠ T142), T118 (≠ L143), D158 (= D182)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
38% identity, 89% coverage: 25:252/256 of query aligns to 4:231/238 of 1lstA

query
sites
1lstA

E

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binding lysine: Y14 (= Y35), F52 (≠ W73), S70 (= S91), S72 (≠ T93), R77 (= R98), L117 (≠ Q139), S120 (≠ T142), T121 (≠ L143), Q122 (= Q144), D161 (= D182)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 89% coverage: 25:252/256 of query aligns to 4:231/238 of 1lahE

query
sites
1lahE

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F

binding L-ornithine: Y14 (= Y35), F52 (≠ W73), S70 (= S91), S72 (≠ T93), R77 (= R98), S120 (≠ T142), Q122 (= Q144), D161 (= D182)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 89% coverage: 25:252/256 of query aligns to 4:231/238 of 1lagE

query
sites
1lagE

E

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L

L

A

T

T

E

L

L

R

R

D

Q

N

D

G

G

T

T

Y

Y

D

D

E

K

I

M

Y

A

N

K

R

K

Y

Y

F

F

binding histidine: Y14 (= Y35), F52 (≠ W73), S69 (= S90), S70 (= S91), L71 (≠ M92), S72 (≠ T93), R77 (= R98), L117 (≠ Q139), S120 (≠ T142), Q122 (= Q144), D161 (= D182)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
38% identity, 89% coverage: 25:252/256 of query aligns to 4:231/238 of 1lafE

query
sites
1lafE

E

Q

E

T

V

V

R

R

I

I

G

G

V

T

D
|
D

V

T

P

T

Y
|
Y

E

A

P

P

M

F

E

S

Y

S

R

K

T

D

P

A

E

K

G

G

E

E

L

F

T

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

E

L

M

C

C

D

K

E

R

I

M

G

Q

V

V

E

K

C

C

E

T

W

W

I

V

V

A

Q

S

G

D

W
x
F

D

D

G

A

I

L

I

I

P

P

G

S

L

L

M

K

S

A

R

K

K

K

Y

I

D

D

A

A

I

I

M

I

S
|
S

M

L

T
x
S

I

I

N

T

D

D

E

K

R
|
R

R

Q

E

Q

Q

E

V

I

L

A

F

F

S

S

D

D

P

K

Y

L

F

Y

T

A

P

A

P

D

S

S

A

R

W

L

F

I

A

A

P

A

A

K

D

G

T

S

D

P

I

I

E

Q

M

-

P

P

Y

T

P

L

Q

E

T

S

L

L

E

K

G

G

K

K

T

H

I

V

G

G

V

V

Q
x
L

R

Q

G

G

T
x
S

L
x
T

Q
|
Q

D

E

N

A

Y

Y

V

A

T

N

D

D

N

N

F

W

G

R

D

T

V

K

A

G

-

V

D

D

V

V

N

V

R

A

Y

Y

A

A

T

N

A

Q

D

D

D

L

M

I

V

Y

L

S

D

D

M

L

D

T

S

A

G

G

R

R

L

L

D

D

I

A

L

A

F

L

L

Q

D
|
D

F

E

P

V

V

A

G

A

K

S

A

E

T

G

L

F

L

L

D

K

S

Q

E

P

E

A

G

G

N

K

-

E

Y

Y

K

A

V

F

V

A

G

G

E

P

M

S

I

V

T

K

E

D

P

-

K

K

E

K

Y

Y

F

F

G

G

D

D

G

G

F

T

G

G

I

V

A

G

F

L

R

R

K

K

R

D

D

D

E

T

A

E

L

L

A

K

E

A

K

A

F

F

N

D

D

K

A

A

L

L

A

T

T

E

L

L

R

R

D

Q

N

D

G

G

T

T

Y

Y

D

D

E

K

I

M

Y

A

N

K

R

K

Y

Y

F

F

binding arginine: D11 (= D32), Y14 (= Y35), F52 (≠ W73), S69 (= S90), S70 (= S91), S72 (≠ T93), R77 (= R98), L117 (≠ Q139), S120 (≠ T142), T121 (≠ L143), Q122 (= Q144), D161 (= D182)

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
34% identity, 89% coverage: 25:252/256 of query aligns to 4:231/238 of 1hslA

query
sites
1hslA

E

Q

E

K

V

I

R

R

I

I

G

G

V

T

D

D

V

P

P

T

Y
|
Y

E

A

P

P

M

F

E

E

Y

S

R

K

T

N

P

A

E

Q

G

G

E

E

L

L

T

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

E

L

L

C

C

D

K

E

R

I

I

G

N

V

T

E

Q

C

C

E

T

W

F

I

V

V

E

Q

N

G

P

W
x
L

D

D

G

A

I

L

I

I

P

P

G

S

L

L

M

K

S

A

R

K

K

K

Y

I

D

D

A

A

I

I

M

M

S
|
S

M
x
L

T
x
S

I

I

N

T

D

E

E

K

R
|
R

R

Q

E

Q

Q

E

V

I

L

A

F

F

S

T

D

D

P

K

Y

L

F

Y

T

A

P

A

P

D

S

S

A

R

W

L

F

V

A

V

P

A

A

K

D

N

T

S

D

D

I

I

E

Q

M

-

P

P

Y

T

P

V

Q

A

T

S

L

L

E

K

G

G

K

K

T

R

I

V

G

G

V

V

Q

L

R

Q

G

G

T
|
T

L
x
T

Q

Q

D

E

N

T

Y

F

V

G

T

N

D

E

N

H

F

W

G

A

D

P

V

K

A

G

-

I

D

E

V

I

N

V

R

S

Y

Y

A

Q

T

G

A

Q

D

D

D

N

M

I

V

Y

L

S

D

D

M

L

D

T

S

A

G

G

R

R

L

I

D

D

I

A

L

A

F

F

L

Q

D
|
D

F

E

P

V

V

A

G

A

K

S

A

E

T

G

L

F

L

L

D

K

S

Q

E

P

E

V

G

G

-

K

N

D

Y

Y

K

K

V

F

V

G

G

G

E

P

M

A

I

V

T

K

E

D

P

E

K

K

E

-

Y

L

F

F

G

G

D

V

G

G

F

T

G

G

I

M

A

G

F

L

R

R

K

K

R

E

D

D

E

N

A

E

L

L

A

R

E

E

K

A

F

L

N

N

D

K

A

A

L

F

A

A

T

E

L

M

R

R

D

A

N

D

G

G

T

T

Y

Y

D

E

E

K

I

L

Y

A

N

K

R

K

Y

Y

F

F

binding histidine: Y14 (= Y35), L52 (≠ W73), S69 (= S90), S70 (= S91), L71 (≠ M92), S72 (≠ T93), R77 (= R98), T120 (= T142), T121 (≠ L143), D161 (= D182)

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
34% identity, 89% coverage: 25:252/256 of query aligns to 26:253/260 of P02910

query
sites
P02910

E

Q

E

K

V

I

R

R

I

I

G

G

V

T

D

D

V

P

P

T

Y

Y

E

A

P

P

M

F

E
|
E

Y

S

R
x
K

T

N

P

A

E

Q

G

G

E
|
E

L

L

T

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

E

L

L

C

C

D

K

E

R

I

I

G

N

V

T

E

Q

C

C

E

T

W

F

I

V

V

E

Q

N

G

P

W

L

D

D

G

A

I

L

I

I

P

P

G

S

L

L

M

K

S

A

R

K

K

K

Y

I

D

D

A

A

I

I

M

M

S

S

M

L

T

S

I

I

N

T

D

E

E

K

R

R

R

Q

E

Q

Q

E

V

I

L

A

F

F

S

T

D

D

P

K

Y

L

F

Y

T

A

P

A

P

D

S

S

A

R

W

L

F

V

A

V

P

A

A

K

D

N

T

S

D

D

I

I

E

Q

M

-

P

P

Y

T

P

V

Q

A

T

S

L

L

E

K

G

G

K

K

T

R

I

V

G

G

V

V

Q

L

R

Q

G

G

T

T

L

T

Q

Q

D

E

N

T

Y

F

V

G

T

N

D

E

N

H

F

W

G

A

D

P

V

K

A

G

-

I

D

E

V

I

N

V

R

S

Y

Y

A

Q

T

G

A

Q

D

D

D

N

M

I

V

Y

L

S

D
|
D

M

L

D

T

S

A

G

G

R
|
R

L

I

D
|
D

I

A

L

A

F

F

L

Q

D

D

F

E

P

V

V

A

G

A

K

S

A

E

T

G

L

F

L

L

D

K

S

Q

E

P

E

V

G

G

-

K

N

D

Y

Y

K

K

V

F

V

G

G

G

E

P

M

A

I

V

T

K

E

D

P

E

K

K

E

-

Y

L

F

F

G

G

D

V

G

G

F

T

G

G

I

M

A

G

F

L

R

R

K

K

R

E

D

D

E

N

A

E

L

L

A

R

E

E

K

A

F

L

N

N

D

K

A

A

L

F

A

A

T

E

L

M

R

R

D

A

N

D

G

G

T

T

Y

Y

D

E

E

K

I

L

Y

A

N

K

R

K

Y

Y

F

F

E40 (= E39) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
K42 (≠ R41) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
E47 (= E46) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
D171 (= D170) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
R176 (= R175) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
D178 (= D177) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.

Sites not aligning to the query:

1:22 signal peptide

P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
34% identity, 89% coverage: 25:252/256 of query aligns to 26:253/260 of P0AEU0

query
sites
P0AEU0

E

Q

E

N

V

I

R

R

I

I

G

G

V

T

D

D

V

P

P

T

Y

Y

E

A

P

P

M

F

E

E

Y

S

R

K

T

N

P

S

E

Q

G

G

E

E

L

L

T

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

E

L

L

C
|
C

D

K

E

R

I

I

G

N

V

T

E

Q

C
|
C

E

T

W

F

I

V

V

E

Q

N

G

P

W

L

D

D

G

A

I

L

I

I

P

P

G

S

L

L

M

K

S

A

R

K

K

K

Y

I

D

D

A

A

I

I

M

M

S
|
S

M

L

T
x
S

I

I

N

T

D

E

E

K

R
|
R

R

Q

E

Q

Q

E

V

I

L

A

F

F

S

T

D

D

P

K

Y

L

F

Y

T

A

P

A

P

D

S

S

A

R

W

L

F

V

A

V

P

A

A

K

D

N

T

S

D

D

I

I

E

Q

M

-

P

P

Y

T

P

V

Q

E

T

S

L

L

E

K

G

G

K

K

T

R

I

V

G

G

V

V

Q

L

R

Q

G

G

T

T

L
x
T

Q

Q

D

E

N

T

Y

F

V

G

T

N

D

E

N

H

F

W

G

A

D

P

V

K

A

G

-

I

D

E

V

I

N

V

R

S

Y

Y

A

Q

T

G

A

Q

D

D

D

N

M

I

V

Y

L

S

D

D

M

L

D

T

S

A

G

G

R

R

L

I

D

D

I

A

L

A

F

F

L

Q

D
|
D

F

E

P

V

V

A

G

A

K

S

A

E

T

G

L

F

L

L

D

K

S

Q

E

P

E

V

G

G

-

K

N

D

Y

Y

K

K

V

F

V

G

G

G

E

P

M

S

I

V

T

K

E

D

P

E

K

K

E

-

Y

L

F

F

G

G

D

V

G

G

F

T

G

G

I

M

A

G

F

L

R

R

K

K

R

E

D

D

E

N

A

E

L

L

A

R

E

E

K

A

F

L

N

N

D

K

A

A

L

F

A

A

T

E

L

M

R

R

D

A

N

D

G

G

T

T

Y

Y

D

E

E

K

I

L

Y

A

N

K

R

K

Y

Y

F

F

C60 (= C59) modified: Disulfide link with 67
C67 (= C66) modified: Disulfide link with 60
S91 (= S90) binding L-histidine
S92 (= S91) binding L-histidine
S94 (≠ T93) binding L-histidine
R99 (= R98) binding L-histidine
T143 (≠ L143) binding L-histidine
D183 (= D182) binding L-histidine

Sites not aligning to the query:

1:22 signal peptide

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
35% identity, 89% coverage: 27:254/256 of query aligns to 6:225/225 of 4zv2A

query
sites
4zv2A

V

L

R

R

I

V

G

G

V

T

D
x
E

V

A

P

T

Y
x
F

E

P

P

P

M

F

E

G

Y

F

R

K

T

D

P

E

E

N

G

G

E

K

L

L

T

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

A

L

I

C

A

D

K

E

K

I

L

G

G

V

V

E

K

C

V

E

E

W

F

I

K

V

P

Q

M

G

D

W
x
F

D

D

G

G

I

I

P

P

G

A

L

L

M

Q

S

S

R

G

K

K

Y

I

D

D

A

V

I

V

M

I

S

A

S
x
G

M
|
M

T
|
T

I

I

N

T

D

E

E

E

R
|
R

R

K

E

K

Q

Q

V

V

L

D

F

F

S

S

D

D

P

P

Y

Y

F

F

T

E

P

A

P

G

S

Q

A

A

W

I

F

V

A

V

P

K

A

K

D

G

T

N

D

D

I

-

E

S

M

I

P

K

Y

S

P

L

Q

E

T

D

L

L

E

K

G

G

K

K

T

K

I

V

G

G

V

V

Q

Q

R

L

G

G

T
x
S

L
x
T

Q

S

D

E

N

Q

Y

H

V

V

T

K

D

K

N

V

F

A

G

A

D

G

V

V

A

-

D

K

V

V

N

K

R

K

Y

F

A

D

T

N

A

F

D

S

D

E

M

A

V

F

L

Q

D

E

M

L

D

K

S

S

G

G

R

R

L

V

D

D

I

A

L

V

F

V

L

T

D
|
D

F

N

P

A

V

V

G

A

K

L

A

A

T

Y

L

V

L

K

D

Q

S

N

E

P

E

N

G

A

N

G

Y

V

K

K

V

I

V

V

G

G

E

E

M

T

I

F

T

S

E

-

P

-

K

-

E

-

Y

-

F

-

G

G

D

E

G

P

F

Y

G

G

I

I

A

A

F

V

R

R

K

K

R

G

D

N

E

S

A

E

L

L

A

L

E

E

K

K

F

I

N

N

D

K

A

A

L

L

A

E

T

E

L

M

R

K

D

K

N

D

G

G

T

T

Y

Y

D

D

E

K

I

I

Y

Y

N

E

R

K

Y

W

F

F

S

G

E

E

binding glutamine: E11 (≠ D32), F14 (≠ Y35), F52 (≠ W73), G70 (≠ S91), M71 (= M92), T72 (= T93), R77 (= R98), S120 (≠ T142), T121 (≠ L143), D159 (= D182)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
35% identity, 88% coverage: 27:252/256 of query aligns to 6:225/226 of 4zv1A

query
sites
4zv1A

V

L

R

R

I

V

G

G

V

T

D
x
E

V

A

P

T

Y
x
F

E

P

P

P

M

F

E

G

Y

F

R

K

T

D

P

E

E

N

G

G

E

K

L

L

T

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

A

L

I

C

A

D

K

E

K

I

L

G

G

V

V

E

K

C

V

E

E

W

F

I

K

V

P

Q

M

G

D

W
x
F

D

D

G

G

I

I

P

P

G

A

L

L

M

Q

S

S

R

G

K

K

Y

I

D

D

A

V

I

V

M

I

S
x
A

S
x
G

M
|
M

T
|
T

I

I

N

T

D

E

E

E

R
|
R

R

K

E

K

Q

Q

V

V

L

D

F

F

S

S

D

D

P

P

Y

Y

F

F

T

E

P

A

P

G

S

Q

A

A

W

I

F

V

A

V

P

K

A

K

D

G

T

N

D

D

I

-

E

S

M

I

P

K

Y

S

P

L

Q

E

T

D

L

L

E

K

G

G

K

K

T

K

I

V

G

G

V

V

Q
|
Q

R

L

G

G

T
x
S

L
x
T

Q

S

D

E

N

Q

Y

H

V

V

T

K

D

K

N

V

F

A

G

K

D

D

V

A

A

G

-

V

D

K

V

V

N

K

R

K

Y

F

A

D

T

N

A

F

D

S

D

E

M

A

V

F

L

Q

D

E

M

L

D

K

S

S

G

G

R

R

L

V

D

D

I

A

L

V

F

V

L

T

D
|
D

F

N

P

A

V

V

G

A

K

L

A

A

T

Y

L

V

L

K

D

Q

S

N

E

P

E

N

G

A

N

G

Y

V

K

K

V

I

V

V

G

G

E

E

M

T

I

F

T

S

E

-

P

-

K

-

E

-

Y

-

F

-

G

G

D

E

G

P

F

Y

G

G

I

I

A

A

F

V

R

R

K

K

R

G

D

N

E

S

A

E

L

L

A

L

E

E

K

K

F

I

N

N

D

K

A

A

L

L

A

E

T

E

L

M

R

K

D

K

N

D

G

G

T

T

Y

Y

D

D

E

K

I

I

Y

Y

N

E

R

K

Y

W

F

F

binding arginine: E11 (≠ D32), F14 (≠ Y35), F52 (≠ W73), A69 (≠ S90), G70 (≠ S91), M71 (= M92), T72 (= T93), R77 (= R98), Q117 (= Q139), S120 (≠ T142), T121 (≠ L143), D161 (= D182)

3tqlA Structure of the amino acid abc transporter, periplasmic amino acid- binding protein from coxiella burnetii. (see paper)
36% identity, 89% coverage: 25:252/256 of query aligns to 2:225/225 of 3tqlA

query
sites
3tqlA

E

D

E

T

V

I

R

K

I

F

G

A

V

T

D
x
E

V

A

P

T

Y
|
Y

E

P

P

P

M

Y

E

V

Y

Y

R

M

T

-

P

-

E

G

G

G

E

Q

L

V

T

E

G

G

F

F

D

G

I

A

D

D

L

I

G

V

N

K

A

A

L

V

C

C

D

K

E

Q

I

M

G

Q

V

A

E

V

C

C

E

T

W

I

I

S

V

N

Q

Q

G

P

W
|
W

D

D

G

S

I

L

I

I

P

P

G

S

L

L

M

K

S

L

R

G

K

K

Y

F

D

D

A

A

I

L

M

F

S
x
G

M

M

T
x
N

I

I

N

T

D

T

E

A

R
|
R

R

Q

E

K

Q

E

V

V

L

D

F

F

S

T

D

D

P

P

Y

Y

F

Y

T

T

P

N

P

S

S

V

A
x
S

W

F

F

I

A

A

P

D

A

K

D

N

T

T

D

P

I

L

E

T

M

L

P

S

Y

-

P

K

Q

Q

T

G

L

L

E

K

G

G

K

K

T

I

I

I

G

G

V

V

Q

Q

R

G

G

G

T

T

L

T

Q

F

D

D

N

S

Y

Y

V

L

T

Q

D

D

N

S

F

F

G

G

D

N

V

S

A

I

D

T

V

I

N

Q

R

R

Y

Y

A

P

T

S

A

E

D

E

D

D

M

A

V

L

L

M

D

D

M

L

D

T

S

S

G

G

R

R

L

V

D

D

I

A

L

V

F

V

L

G

D

D

F

T

P

P

V

L

G

I

K

K

A

Q

T

W

L

L

L

K

D

Q

S

N

E

G

E

R

G

R

N

E

Y

Y

K

V

V

L

V

I

G

G

E

K

M

P

I

V

T

N

E

D

P

P

K

-

E

N

Y

Y

F

F

G

G

D

K

G

G

F

V

G

G

I

I

A

A

F

V

R

K

K

K

R

G

D

N

E

Q

A

A

L

L

A

L

E

L

K

K

F

L

N

N

D

K

A

A

L

L

A

A

T

A

L

I

R

K

D

A

N

N

G

G

T

V

Y

Y

D

A

E

A

I

I

Y

V

N

Q

R

K

Y

Y

F

F

binding arginine: E9 (≠ D32), Y12 (= Y35), W48 (= W73), G65 (≠ S90), G66 (≠ S91), N68 (≠ T93), R73 (= R98), S89 (≠ A114)

5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
30% identity, 90% coverage: 23:252/256 of query aligns to 1:249/255 of 5itoA

query
sites
5itoA

D

D

Y

Y

E

K

E

S

V

I

R

T

I

I

G

A

V

T

D
x
E

V

G

P

S

Y
|
Y

E

A

P

P

M

Y

E

N

Y

F

R

K

T

D

P

A

E

G

G

G

E

K

L

L

T

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

L

L

C

C

D

K

E

R

I

M

G

N

V

I

E

E

C

C

E

K

W

F

I

V

V

E

Q

Q

G

A

W
|
W

D

D

G

G

I

I

P

P

G

S

L

L

M

T

S

A

R

G

K

R

Y

Y

D

D

A

A

I

I

M

M

S
x
A

M

M

T
x
G

I

I

N

Q

D

P

E

A

R
|
R

R

E

E

K

Q

V

V

I

L

A

F

F

S

S

D

R

P

P

Y

Y

F

L

T

L

P

T

P

P

S

N

A

T

W

F

F

L

A

T

P

T

A

A

D

D

T

S

D

P

I

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

E

N

M

L

P

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

Y

F

P

T

Q

K

T

I

L

F

E

E

G

G

K

V

T

K

I

F

G

G

V

V

Q
|
Q

R

A

G

G

T
|
T

L
x
S

Q
x
H

D

E

N

A

Y

F

V

M

T

K

D

Q

N

M

F

M

G

P

D

S

V

V

A

-

D

Q

V

I

N

S

R

T

Y

Y

A

D

T

T

A

I

D

D

D

N

M

V

V

V

L

M

D

D

M

L

D

K

S

A

G

G

R

R

L

I

D

D

I

A

L

S

F

L

L

A

D
x
S

F

V

P

S

V

F

G

L

K

K

A

P

T

L

L

T

L

D

D

K

S

P

E

D

E

N

G

K

N

D

Y

L

K

K

V

M

V

F

G

G

E

P

M

R

I

M

T

T

E

-

P

-

K

G

E

G

Y

L

F

F

G

G

D

K

G

G

F

V

G

G

I

V

A

G

F

I

R

R

K

K

R

E

D

D

E

A

A

D

L

L

A

K

E

A

K

L

F

F

N

D

D

K

A

A

L

I

A

D

T

A

L

A

R

I

D

A

N

D

G

G

T

T

Y

V

D

Q

E

K

I

L

Y

S

N

Q

R

Q

Y

W

F

F

binding octopine: E10 (≠ D32), Y13 (= Y35), W51 (= W73), A68 (≠ S90), A69 (≠ S91), G71 (≠ T93), R76 (= R98), Q139 (= Q139), T142 (= T142), S143 (≠ L143), H144 (≠ Q144), S181 (≠ D182)

5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
30% identity, 90% coverage: 23:252/256 of query aligns to 1:249/259 of 5ovzA

query
sites
5ovzA

D

D

Y

Y

E

K

E

S

V

I

R

T

I

I

G

A

V

T

D
x
E

V

G

P

S

Y
|
Y

E

A

P

P

M

Y

E

N

Y

F

R

K

T

D

P

A

E

G

G

G

E

K

L

L

T

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

L

L

C

C

D

K

E

R

I

M

G

N

V

I

E

E

C

C

E

K

W

F

I

V

V

E

Q

Q

G

A

W
|
W

D

D

G

G

I

I

P

P

G

S

L

L

M

T

S

A

R

G

K

R

Y

Y

D

D

A

A

I

I

M

M

S
x
A

M

M

T
x
G

I

I

N

Q

D

P

E

A

R
|
R

R

E

E

K

Q

V

V

I

L

A

F

F

S

S

D

R

P

P

Y

Y

F

L

T

L

P

T

P

P

S
x
M

A

T

W

F

F

L

A

T

P

T

A

A

D

D

T

S

D

P

I

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

E

N

M

L

P

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

Y

F

P

T

Q

K

T

I

L

F

E

E

G

G

K

V

T

K

I

F

G

G

V

V

Q
|
Q

R

A

G

G

T
|
T

L
x
S

Q
x
H

D

E

N

A

Y

F

V

M

T

K

D

Q

N

M

F

M

G

P

D

S

V

V

A

-

D

Q

V

I

N

S

R

T

Y

Y

A

D

T

T

A

I

D

D

D

N

M

V

V

V

L

M

D

D

M

L

D

K

S

A

G

G

R

R

L

I

D

D

I

A

L

S

F

L

L

A

D
x
S

F

V

P

S

V

F

G

L

K

K

A

P

T

L

L

T

L

D

D

K

S

P

E

D

E

N

G

K

N

D

Y

L

K

K

V

M

V

F

G

G

E

P

M

R

I

M

T

T

E

-

P

-

K

G

E

G

Y

L

F

F

G

G

D

K

G

G

F
x
V

G

G

I

V

A

G

F

I

R

R

K

K

R

E

D

D

E

A

A

D

L

L

A

K

E

A

K

L

F

F

N

D

D

K

A

A

L

I

A

D

T

A

L

A

R

I

D

A

N

D

G

G

T

T

Y

V

D

Q

E

K

I

L

Y

S

N

Q

R

Q

Y

W

F

F

binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: E10 (≠ D32), Y13 (= Y35), W51 (= W73), A68 (≠ S90), A69 (≠ S91), G71 (≠ T93), R76 (= R98), M91 (≠ S113), Q139 (= Q139), T142 (= T142), S143 (≠ L143), H144 (≠ Q144), S181 (≠ D182), V213 (≠ F216)

5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
30% identity, 89% coverage: 24:252/256 of query aligns to 1:248/254 of 5otaA

query
sites
5otaA

Y

Y

E

K

E

S

V

I

R

T

I

I

G

A

V

T

D
x
E

V

G

P

S

Y
|
Y

E

A

P

P

M

Y

E

N

Y

F

R

K

T

D

P

A

E

G

G

G

E

K

L

L

T

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

L

L

C

C

D

K

E

R

I

M

G

N

V

I

E

E

C

C

E

K

W

F

I

V

V

E

Q

Q

G

A

W
|
W

D

D

G

G

I

I

P

P

G

S

L

L

M

T

S

A

R

G

K

R

Y

Y

D

D

A

A

I

I

M

M

S

A

S
x
A

M

M

T
x
G

I

I

N

Q

D

P

E

A

R
|
R

R

E

E

K

Q

V

V

I

L

A

F

F

S

S

D

R

P

P

Y

Y

F

L

T

L

P

T

P

P

S
x
M

A

T

W

F

F

L

A

T

P

T

A

A

D

D

T

S

D

P

I

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

E

N

M

L

P

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

Y

F

P

T

Q

K

T

I

L

F

E

E

G

G

K

V

T

K

I

F

G

G

V

V

Q
|
Q

R

A

G

G

T
|
T

L
x
S

Q
x
H

D

E

N

A

Y

F

V

M

T

K

D

Q

N

M

F

M

G

P

D

S

V

V

A

-

D

Q

V

I

N

S

R

T

Y

Y

A

D

T

T

A

I

D

D

D

N

M

V

V

V

L

M

D

D

M

L

D

K

S

A

G

G

R

R

L

I

D

D

I

A

L

S

F

L

L

A

D
x
S

F

V

P

S

V

F

G

L

K

K

A

P

T

L

L

T

L

D

D

K

S

P

E

D

E

N

G

K

N

D

Y

L

K

K

V

M

V

F

G

G

E

P

M

R

I

M

T

T

E

-

P

-

K

G

E

G

Y

L

F

F

G

G

D

K

G

G

F

V

G

G

I

V

A

G

F

I

R

R

K

K

R

E

D

D

E

A

A

D

L

L

A

K

E

A

K

L

F

F

N

D

D

K

A

A

L

I

A

D

T

A

L

A

R

I

D

A

N

D

G

G

T

T

Y

V

D

Q

E

K

I

L

Y

S

N

Q

R

Q

Y

W

F

F

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: E9 (≠ D32), Y12 (= Y35), W50 (= W73), A68 (≠ S91), G70 (≠ T93), R75 (= R98), M90 (≠ S113), Q138 (= Q139), T141 (= T142), S142 (≠ L143), H143 (≠ Q144), S180 (≠ D182)

5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
30% identity, 89% coverage: 24:252/256 of query aligns to 1:248/254 of 5ot9A

query
sites
5ot9A

Y

Y

E

K

E

S

V

I

R

T

I

I

G

A

V

T

D
x
E

V

G

P

S

Y
|
Y

E

A

P

P

M

Y

E

N

Y

F

R

K

T

D

P

A

E

G

G

G

E

K

L

L

T

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

L

L

C

C

D

K

E

R

I

M

G

N

V

I

E

E

C

C

E

K

W

F

I

V

V

E

Q

Q

G

A

W
|
W

D

D

G

G

I

I

P

P

G

S

L

L

M

T

S

A

R

G

K

R

Y

Y

D

D

A

A

I

I

M

M

S
x
A

M

M

T

G

I

I

N

Q

D

P

E

A

R
|
R

R

E

E

K

Q

V

V

I

L

A

F

F

S

S

D

R

P

P

Y

Y

F

L

T

L

P

T

P

P

S
x
M

A

T

W

F

F

L

A

T

P

T

A

A

D

D

T

S

D

P

I

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

E

N

M

L

P

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

Y

F

P

T

Q

K

T

I

L

F

E

E

G

G

K

V

T

K

I

F

G

G

V

V

Q
|
Q

R

A

G

G

T
|
T

L
x
S

Q
x
H

D

E

N

A

Y

F

V

M

T

K

D

Q

N

M

F

M

G

P

D

S

V

V

A

-

D

Q

V

I

N

S

R

T

Y

Y

A

D

T

T

A

I

D

D

D

N

M

V

V

V

L

M

D

D

M

L

D

K

S

A

G

G

R

R

L

I

D

D

I

A

L

S

F

L

L

A

D
x
S

F

V

P

S

V

F

G

L

K

K

A

P

T

L

L

T

L

D

D

K

S

P

E

D

E

N

G

K

N

D

Y

L

K

K

V

M

V

F

G

G

E

P

M

R

I

M

T

T

E

-

P

-

K

G

E

G

Y

L

F

F

G

G

D

K

G

G

F

V

G

G

I

V

A

G

F

I

R

R

K

K

R

E

D

D

E

A

A

D

L

L

A

K

E

A

K

L

F

F

N

D

D

K

A

A

L

I

A

D

T

A

L

A

R

I

D

A

N

D

G

G

T

T

Y

V

D

Q

E

K

I

L

Y

S

N

Q

R

Q

Y

W

F

F

binding Histopine: E9 (≠ D32), Y12 (= Y35), W50 (= W73), A67 (≠ S90), A68 (≠ S91), R75 (= R98), M90 (≠ S113), Q138 (= Q139), T141 (= T142), S142 (≠ L143), H143 (≠ Q144), S180 (≠ D182)

5orgA Structure of the periplasmic binding protein (pbp) occj from a. Tumefaciens b6 in complex with octopine. (see paper)
30% identity, 89% coverage: 25:252/256 of query aligns to 4:250/257 of 5orgA

query
sites
5orgA

E

K

E

S

V

I

R

T

I

I

G

A

V

T

D
x
E

V

G

P

G

Y
|
Y

E

A

P

P

M

W

E

N

Y

F

R

S

T

G

P

P

E

G

G

G

E

K

L

L

T

D

G

G

F

F

D

E

I

I

D

D

L

L

G

A

N

N

A

A

L

L

C

C

D

E

E

K

I

M

G

K

V

A

E

K

C

C

E

Q

W

I

I

V

V

A

Q

Q

G

N

W
|
W

D

D

G

G

I

I

I

M

P

P

G

S

L

L

M

T

S

G

R

K

K

K

Y

Y

D

D

A

A

I

I

M

M

S
x
A

M
|
M

T
x
S

I

V

N

T

D

P

E

K

R
|
R

R

Q

E

E

Q

V

V

I

L

G

F

F

S

S

D

I

P

P

Y

Y

F

A

T

A

P

G

P

I

S
x
N

A

G

W

F

F

A

A

V

P

M

A

G

D

D

T

S

D

K

I

L

-

A

E

E

M

M

P

P

-

G

-

L

-

G

-

E

-

T

-

Y

-

S

-

L

-

D

-

S

-

Q

-

A

-

D

-

A

-

K

-

A

-

I

-

A

-

D

Y

I

P

S

Q

S

T

F

L

L

E

N

G

G

K

T

T

T

I

V

G

G

V

V

Q
|
Q

R

G

G

S

T
|
T

L
x
T

Q

A

D

S

N

T

Y

F

V

L

T

D

D

K

N

Y

F

F

G

K

D

G

V

S

A

V

D

D

V

I

N

K

R

E

Y

Y

A

K

T

S

A

V

D

E

M

H

V

N

L

L

D

D

M

L

D

T

S

S

G

G

R

R

L

L

D

D

I

A

L

V

F

L

L

A

D
x
N

F

A

P

T

V

V

G

L

K

A

A

A

T

A

L

I

L

E

D

K

S

P

E

E

E

M

G

K

N

G

Y

A

K

K

V

L

V

V

G

G

E

P

M

L

I

-

T

-

E

-

P

-

K

-

E

-

Y

F

F

S

G

G

D

G

G

E

F

F

G

G

-

V

-

A

I

V

A

G

F

L

R

R

K

K

R

E

D

D

E

T

A

A

L

L

A

K

E

A

K

D

F

F

N

D

D

A

A

A

L

I

A

K

T

A

L

A

R

S

D

E

N

D

G

G

T

T

Y

I

D

K

E

T

I

L

Y

S

N

L

R

K

Y

W

F

F

binding octopine: E11 (≠ D32), Y14 (= Y35), W52 (= W73), A69 (≠ S90), A70 (≠ S91), M71 (= M92), S72 (≠ T93), R77 (= R98), N92 (≠ S113), Q140 (= Q139), T143 (= T142), T144 (≠ L143), N183 (≠ D182)

4powA Structure of the pbp noct in complex with pyronopaline (see paper)
30% identity, 89% coverage: 24:252/256 of query aligns to 1:248/254 of 4powA

query
sites
4powA

Y

Y

E

K

E

S

V

I

R

T

I

I

G

A

V

T

D
x
E

V

G

P

S

Y
|
Y

E

A

P

P

M

Y

E

N

Y

F

R

K

T

D

P

A

E

G

G

G

E

K

L

L

T

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

L

L

C

C

D

K

E

R

I

M

G

N

V

I

E

E

C

C

E

K

W

F

I

V

V

E

Q

Q

G

A

W
|
W

D

D

G

G

I

I

P

P

G

S

L

L

M

T

S

A

R

G

K

R

Y

Y

D

D

A

A

I

I

M

M

S
x
A

M

M

T
x
G

I

I

N

Q

D

P

E

A

R
|
R

R

E

E

K

Q

V

V

I

L

A

F

F

S

S

D

R

P

P

Y

Y

F

L

T

L

P

T

P

P

S
x
M

A

T

W

F

F

L

A

T

P

T

A

A

D

D

T

S

D

P

I

L

-

L

-

K

-

T

-

Q

-

V

-

A

-

I

-

E

E

N

M

L

P

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

Y

F

P

T

Q

K

T

I

L

F

E

E

G

G

K

V

T

K

I

F

G

G

V

V

Q
|
Q

R

A

G

G

T
|
T

L
x
S

Q
x
H

D

E

N

A

Y

F

V

M

T

K

D

Q

N

M

F

M

G

P

D

S

V

V

A

-

D

Q

V

I

N

S

R

T

Y

Y

A

D

T

T

A

I

D

D

D

N

M

V

V

V

L

M

D

D

M

L

D

K

S

A

G

G

R

R

L

I

D

D

I

A

L

S

F

L

L

A

D
x
S

F

V

P

S

V

F

G

L

K

K

A

P

T

L

L

T

L

D

D

K

S

P

E

D

E

N

G

K

N

D

Y

L

K

K

V

M

V

F

G

G

E

P

M

R

I

M

T

T

E

-

P

-

K

G

E

G

Y

L

F

F

G

G

D

K

G

G

F

V

G

G

I

V

A

G

F

I

R

R

K

K

R

E

D

D

E

A

A

D

L

L

A

K

E

A

K

L

F

F

N

D

D

K

A

A

L

I

A

D

T

A

L

A

R

I

D

A

N

D

G

G

T

T

Y

V

D

Q

E

K

I

L

Y

S

N

Q

R

Q

Y

W

F

F

binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: E9 (≠ D32), Y12 (= Y35), W50 (= W73), A67 (≠ S90), A68 (≠ S91), G70 (≠ T93), R75 (= R98), M90 (≠ S113), Q138 (= Q139), T141 (= T142), S142 (≠ L143), H143 (≠ Q144), S180 (≠ D182)

5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
30% identity, 89% coverage: 24:252/256 of query aligns to 1:248/256 of 5otcA

query
sites
5otcA

Y

Y

E

K

E

S

V

I

R

T

I

I

G

A

V

T

D
x
E

V

G

P

S

Y
|
Y

E

A

P

P

M

Y

E

N

Y

F

R

K

T

D

P

A

E

G

G

G

E

K

L

L

T

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

L

L

C

C

D

K

E

R

I

M

G

N

V

I

E

E

C

C

E

K

W

F

I

V

V

E

Q

Q

G

A

W
|
W

D

D

G

G

I

I

P

P

G

S

L

L

M

T

S

A

R

G

K

R

Y

Y

D

D

A

A

I

I

M

M

S

A

S
x
A

M

M

T
x
G

I

I

N

Q

D

P

E

A

R
|
R

R

E

E

K