SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043743277.1 AMB_RS02850 branched-chain amino acid aminotransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
45% identity, 92% coverage: 8:274/290 of query aligns to 4:275/306 of 4whxA

query
sites
4whxA

D

D

R

R

D

D

G

G

F

K

L

I

W

W

F

M

D

D

G

G

K

K

L

L

V

I

P

E

W

W

R

R

D

D

A

A

K

K

I

I

H

H

V

V

L

L

T

T

H

H

G

T

L

L

H

H

Y

Y

G

G

S

M

C

G

V

V

F
|
F

E

E

G

G

E

V

R

R

V

A

Y

Y

-

K

-

T

-

A

-

D

-

G

G

G

G

T

K

A

V

I

F

F

K

R

L

L

R

K

E

E

H

H

S

T

Q

K

R

R

L

L

I

L

D

N

S

S

G

A

R

K

I

I

L

F

G

Q

F

M

E

D

V

V

P

P

W

F

T

D

V

Q

E

E

E

T

I

L

D

E

E

A

A

A

T

Q

M

R

A

D

T

V

V

V

K

R

A

E

N

N

N

K

I

L

V

E

D

S

G

C

Y
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Y

V

L

R

R

P

P

V

I

A

I

W

W

R

I

G

G

S

S

E

E

M

K

M

L

G

G

V

V

A

S

A

A

Q

K

T

G

T

N

K

T

I

I

R

H

F

V

A

A

V

I

A

A

T

A

W

W

S

P

W

W

P

G

S

A

Y

Y

W

L

S

G

P

E

E

E

A

G

R

L

M

A

K

K

G

G

I

I

R

R

L

V

N

K

I

T

S

S

T

S

W

F

K

T

R

R

P

H

H

H

P

V

E

N

T

V

A

S

P

M

T

V

A

R

S

A

K
|
K

A

A

A

S

G

G

L

W

Y

Y

M

V

I

N

C

S

T

I

M

L

S

A

K

N

H

Q

K

E

A

A

E

T

G

A

D

D

G

G

Y

Y

E

D

D

E

S

A

L

L

M

L

L

L

D

D

W

V

R

D

G

G

Q

Y

V

V

A

S

E
|
E

A

G

T

S

G
|
G

A

E

N

N

I

F

F

F

F

L

V

V

F

N

G

R

N

G

E

K

L

L

H

Y

T

T

P

P

T

D

P

L

D

A

C

S

F

C

L
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L

N

D

G

G

I

I

T

T

R

R

Q

D

T

T

V

V

I

I

A

T

L

L

A

A

K

K

K

E

R

A

G

G

I

I

T

E

V

V

V

I

E

E

R

K

A

R

I

I

F

T

P

R

E

D

E

E

M

V

T

Y

K

T

A

A

S

D

E

E

C

A

F

F

L

F

T

T

G

G

T
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T

A
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A

A

A

E

E

V

V

T

T

P

P

V

I

R

R

E

E

I

L

G

D

P

N

Y

R

T

T

F

I

T

G

P

G

G

G

active site: F37 (= F41), K160 (= K159), E194 (= E193), L217 (= L216)
binding alanine: Y97 (= Y96), K160 (= K159), G197 (= G196), T257 (= T256), A258 (= A257)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
41% identity, 88% coverage: 12:266/290 of query aligns to 4:264/304 of 1iyeA

query
sites
1iyeA

F

Y

L

I

W

W

F

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

K

K

I

V

H

H

V

V

L

M

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

C

Y

Y

-

D

-

S

-

H

-

K

G

G

G

P

K

V

V

V

F

F

K

R

L

H

R

R

E

E

H

H

S

M

Q

Q

R
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R

L

L

I

H

D

D

S

S

G

A

R

K

I

I

L

Y

G

R

F

F

E

P

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V

P

S

W

Q

T

S

V

I

E

D

E

E

I

L

D

M

E

E

A

A

T

C

M

R

A

D

T

V

V

I

K

R

A

K

N

N

I

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D

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G

A

Y
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Y

V

I

R
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R

P

P

V

L

A

I

W

F

R

V

G

G

S

D

E

V

M

G

M

M

G

G

V

V

-

N

-

P

A

A

A

G

Q

Y

T

S

T

T

K

D

I

V

R

-

F

-

A

I

V

I

A

A

T

A

W

F

S

P

W

W

P

G

S

A

Y
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Y

W

L

S

G

P

A

E

E

A

A

R

L

M

E

K

Q

G

G

I

I

R

D

L

A

N

M

I

V

S

S

T

S

W

W

K

N

R

R

P

A

H

A

P

P

E

N

T

T

A

I

P

P

T

T

A

A

S

A

K
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K

A
|
A

A

G

G

G

L

N

Y
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Y

M

L

I

S

C

S

T

L

M

L

S

V

K

G

H

S

K

E

A

A

E

R

G

R

D

H

G

G

Y

Y

E

Q

D

E

S

G

L

I

M

A

L

L

D

D

W

V

R

N

G

G

Q

Y

V

I

A

S

E
|
E

A

G

T
x
A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

F

K

G

D

N

G

E

V

L

L

H

F

T

T

P

P

T

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

A

K

L

L

A

A

K

K

K

E

R

L

G

G

I

I

T

E

V

V

V

R

E

E

R

Q

A

V

I

L

F

S

P

R

E

E

E

S

M

L

T

Y

K

L

A

A

S

D

E

E

C

V

F

F

L

M

T

S

G
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G

T
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T

A
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A

A

A

E

E

V

I

T

T

P

P

V

V

R

R

E

S

I

V

active site: F33 (= F41), G35 (= G43), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), R94 (= R98), Y126 (= Y129), K156 (= K159), Y161 (= Y164), E190 (= E193), A192 (≠ T195), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
41% identity, 88% coverage: 12:266/290 of query aligns to 4:264/304 of 1iydA

query
sites
1iydA

F

Y

L

I

W

W

F

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

K

K

I

V

H

H

V

V

L

M

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

C

Y

Y

-

D

-

S

-

H

-

K

G

G

G

P

K

V

V

V

F

F

K

R

L

H

R

R

E

E

H

H

S

M

Q

Q

R
|
R

L

L

I

H

D

D

S

S

G

A

R

K

I

I

L

Y

G

R

F

F

E

P

V

V

P

S

W

Q

T

S

V

I

E

D

E

E

I

L

D

M

E

E

A

A

T

C

M

R

A

D

T

V

V

I

K

R

A

K

N

N

I

L

V

T

D

S

G

A

Y
|
Y

V

I

R
|
R

P

P

V

L

A

I

W

F

R

V

G

G

S

D

E

V

M

G

M

M

G

G

V

V

-

N

-

P

A

A

A

G

Q

Y

T

S

T

T

K

D

I

V

R

-

F

-

A

I

V

I

A

A

T

A

W

F

S

P

W
|
W

P

G

S

A

Y
|
Y

W

L

S

G

P

A

E

E

A

A

R

L

M

E

K

Q

G

G

I

I

R

D

L

A

N

M

I

V

S

S

T

S

W

W

K

N

R

R

P

A

H

A

P

P

E

N

T

T

A

I

P

P

T

T

A

A

S

A

K
|
K

A
|
A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

M

L

S

V

K

G

H

S

K

E

A

A

E

R

G

R

D

H

G

G

Y

Y

E

Q

D

E

S

G

L

I

M

A

L

L

D

D

W

V

R

N

G

G

Q

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

F

K

G

D

N

G

E

V

L

L

H

F

T

T

P

P

T

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
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I

T
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T

R

R

Q

D

T

A

V

I

I

I

A

K

L

L

A

A

K

K

K

E

R

L

G

G

I

I

T

E

V

V

V

R

E

E

R

Q

A

V

I

L

F

S

P

R

E

E

E

S

M

L

T

Y

K

L

A

A

S

D

E

E

C

V

F

F

L

M

T

S

G
|
G

T
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T

A
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A

A

A

E

E

V

I

T

T

P

P

V

V

R

R

E

S

I

V

active site: F33 (= F41), G35 (= G43), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
binding glutaric acid: G35 (= G43), Y92 (= Y96), R94 (= R98), W123 (= W126), Y126 (= Y129), K156 (= K159), Y161 (= Y164), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
41% identity, 88% coverage: 12:266/290 of query aligns to 4:264/304 of 1i1mA

query
sites
1i1mA

F

Y

L

I

W

W

F

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

K

K

I

V

H

H

V

V

L

M

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F

F

E

E

G
|
G

E

I

R

R

V

C

Y

Y

-

D

-

S

-

H

-

K

G

G

G

P

K

V

V

V

F

F

K

R

L

H

R

R

E

E

H

H

S

M

Q

Q

R
|
R

L

L

I

H

D

D

S

S

G

A

R

K

I

I

L

Y

G

R

F

F

E

P

V

V

P

S

W

Q

T

S

V

I

E

D

E

E

I

L

D

M

E

E

A

A

T

C

M

R

A

D

T

V

V

I

K

R

A

K

N

N

I

L

V

T

D

S

G

A

Y
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Y

V

I

R

R

P

P

V

L

A

I

W

F

R

V

G

G

S

D

E

V

M

G

M

M

G

G

V

V

-

N

-

P

A

A

A

G

Q

Y

T

S

T

T

K

D

I

V

R

-

F

-

A

I

V

I

A

A

T

A

W

F

S

P

W

W

P

G

S

A

Y

Y

W

L

S

G

P

A

E

E

A

A

R

L

M

E

K

Q

G

G

I

I

R

D

L

A

N

M

I

V

S

S

T

S

W

W

K

N

R
|
R

P

A

H

A

P

P

E

N

T

T

A

I

P

P

T

T

A

A

S

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

M

L

S

V

K

G

H

S

K

E

A

A

E

R

G

R

D

H

G

G

Y

Y

E

Q

D

E

S

G

L

I

M

A

L

L

D

D

W

V

R

N

G

G

Q

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

F

K

G

D

N

G

E

V

L

L

H

F

T

T

P

P

T

P

-

F

P

T

D

S

C

S

F

A

L
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L

N

P

G
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G

I
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I

T
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T

R

R

Q

D

T

A

V

I

I

I

A

K

L

L

A

A

K

K

K

E

R

L

G

G

I

I

T

E

V

V

V

R

E

E

R

Q

A

V

I

L

F

S

P

R

E

E

E

S

M

L

T

Y

K

L

A

A

S

D

E

E

C

V

F

F

L

M

T

S

G
|
G

T
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T

A
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A

A

A

E

E

V

I

T

T

P

P

V

V

R

R

E

S

I

V

active site: K156 (= K159)
binding 4-methyl valeric acid: G35 (= G43), Y92 (= Y96), K156 (= K159), Y161 (= Y164), G193 (= G196), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), R145 (= R148), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
41% identity, 88% coverage: 12:266/290 of query aligns to 4:264/304 of 1i1lA

query
sites
1i1lA

F

Y

L

I

W

W

F

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

K

K

I

V

H

H

V

V

L

M

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F

F

E

E

G
|
G

E

I

R

R

V

C

Y

Y

-

D

-

S

-

H

-

K

G

G

G

P

K

V

V

V

F

F

K

R

L

H

R

R

E

E

H

H

S

M

Q

Q

R
|
R

L

L

I

H

D

D

S

S

G

A

R

K

I

I

L

Y

G

R

F

F

E

P

V

V

P

S

W

Q

T

S

V

I

E

D

E

E

I

L

D

M

E

E

A

A

T

C

M

R

A

D

T

V

V

I

K

R

A

K

N

N

I

L

V

T

D

S

G

A

Y
|
Y

V

I

R

R

P

P

V

L

A

I

W

F

R

V

G

G

S

D

E

V

M

G

M

M

G

G

V

V

-

N

-

P

A

A

A

G

Q

Y

T

S

T

T

K

D

I

V

R

-

F

-

A

I

V

I

A

A

T

A

W

F

S

P

W
|
W

P

G

S

A

Y
|
Y

W

L

S

G

P

A

E

E

A

A

R

L

M

E

K

Q

G

G

I

I

R

D

L

A

N

M

I

V

S

S

T

S

W

W

K

N

R

R

P

A

H

A

P

P

E

N

T

T

A

I

P

P

T

T

A

A

S

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

M

L

S

V

K

G

H

S

K

E

A

A

E

R

G

R

D

H

G

G

Y

Y

E

Q

D

E

S

G

L

I

M

A

L

L

D

D

W

V

R

N

G

G

Q

Y

V

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N
|
N

I

L

F

F

F

E

V

V

F

K

G

D

N

G

E

V

L

L

H

F

T

T

P

P

T

P

-

F

P

T

D

S

C

S

F

A

L
|
L

N

P

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

A

V

I

I

I

A

K

L

L

A

A

K

K

K

E

R

L

G

G

I

I

T

E

V

V

V

R

E

E

R

Q

A

V

I

L

F

S

P

R

E

E

E

S

M

L

T

Y

K

L

A

A

S

D

E

E

C

V

F

F

L

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

R

R

E

S

I

V

active site: K156 (= K159)
binding 2-methylleucine: G35 (= G43), Y92 (= Y96), W123 (= W126), Y126 (= Y129), K156 (= K159), G193 (= G196), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
38% identity, 95% coverage: 13:288/290 of query aligns to 9:286/301 of 6thqB

query
sites
6thqB

L

V

W

W

F

L

D

D

G

G

K

R

L

L

V

V

P

D

W

E

R

E

D

E

A

A

K

K

I

V

H

T

V

V

L

L

T

S

H

P

G

S

L

L

H

N

Y
|
Y

G

G

S

F

C

G

V

V

F
|
F

E

E

G

G

E

I

R

R

V

A

Y

Y

-

W

G

N

G

G

K

E

-

N

-

L

-

Y

V

V

F

F

K

R

L

L

R

R

E

D

H

H

S

M

Q

E

R
|
R

L

L

I

L

D

R

S

S

G

A

R

K

I

I

L

I

G

G

F

L

E

D

V

V

P

P

W

Y

T

T

V

A

E

E

I

L

D

S

E

K

A

A

T

V

M

V

A

E

T

T

V

V

K

R

A

A

N

N

N

G

I

F

V

K

-

E

D

D

G

L

Y
|
Y

V

I

R

R

P

P

V

V

A

A

W

Y

R

I

G

S

S

K

E

P

M

Q

M
x
I

G

S

V

L

A

D

A

V

Q

R

T

G

T

L

K

Q

I

A

R

S

F

V

A

A

V

I

A

A

T

A

W

I

S

P

W

F

P

G

S

K

Y

Y

W

L

S

-

P

-

E

-

A

-

R

K

M

V

K

E

G

G

I

V

R

R

L

A

N

A

I

V

S

V

T

S

W

W

K

R

R
|
R

P

V

H

H

P

T

E

S

T

M

A

M

P

P

T

V

A

M

S

A

K
|
K

A

A

A

T

G

G

L

I

Y
|
Y

M

L

I

N

C

S

T

I

M

M

S

A

K

A

H

V

K

E

A

A

E

R

G

A

D

R

G

G

Y

Y

E

D

D

E

S

A

L

I

M

M

L

L

D

N

W

A

R

E

G

G

Q

K

V

V

A

V

E
|
E

A

G

T
x
S

G
|
G

A
x
E

N
|
N

I

I

F

F

F

I

V

V

F

R

G

R

N

G

E

V

L

L

H

M

T

T

P

P

-

P

T

L

P

E

D

D

C

G

F

I

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

E

T

T

V

V

I

I

A

S

L

I

A

A

K

G

K

D

R

L

G

G

I

I

T

P

V

L

E

E

R

K

A

S

I

I

F

T

P

R

E

E

M

L

T

Y

K

A

A

A

S

D

E

E

C

A

F

F

L

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

I

R

I

E

E

I

I

G

D

P

G

Y

R

T

V

F

L

T

Q

P

R

G

G

D

P

I

I

T

T

R

Q

Q

K

L

I

I

A

T

E

D

T

Y

Y

D

R

A

R

L

I

V

V

active site: F37 (= F41), K156 (= K159), E190 (= E193), L214 (= L216)
binding pyridoxal-5'-phosphate: R60 (= R60), R145 (= R148), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: Y32 (= Y36), R60 (= R60), Y97 (= Y96), I109 (≠ M108), K156 (= K159), Y161 (= Y164), E190 (= E193), S192 (≠ T195), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
39% identity, 91% coverage: 9:273/290 of query aligns to 3:277/305 of 2ej0B

query
sites
2ej0B

R

K

D

A

G

G

F

L

L

I

W

W

F

M

D

N

G

G

K

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

K

K

I

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

-

E

-

T

-

A

-

K

G

G

G

P

K

A

V

I

F

F

K

R

L

L

R

K

E

E

H

H

S

V

Q

K

R
|
R

L

F

I

Y

D

N

S

S

G

A

R

K

I

V

L

L

G

R

F

M

E

E

V

I

P

P

W

F

T

A

V

P

E

E

I

L

D

E

E

E

A

A

T

I

M

K

A

E

T

V

V

V

K

R

A

R

N

N

N

G

I

Y

V

R

D

S

G

C

Y

Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

-

L

Q

P

T

N

K

P

I

A

R

E

F

V

A

M

V

V

A

A

T

A

W

W

S

E

W

W

P

G

S

A

Y

Y

W

L

S

G

P

E

E

E

A

A

R

V

M

R

K

K

G

G

I

A

R

R

L

L

N

I

I

T

S

S

T

S

W

W

K

A

R

R

P

F

H

P

P

A

E

N

T

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

M

L

S

A

K

K

H

M

K

E

A

A

E

V

G

A

D

A

G

G

Y

A

E

D

D

E

S

A

L

L

M

L

L

L

D

D

W

E

R

E

G

G

Q

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N
|
N

I

L

F

F

V

V

F

R

G

D

N

G

E

V

L

I

H

Y

T

A

P

L

T

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

A

R

L

I

A

A

K

K

K

D

R

L

G

G

I

Y

T

E

V

V

V

Q

E

V

R

V

A

R

I

A

F

T

P

R

E

D

E

Q

M

L

T

Y

K

M

A

A

S

D

E

E

C

V

F

F

L

M

T

T

G
|
G

T
|
T

A

A

E

E

V

V

T

T

P

P

V

V

-

S

-

M

-

I

-

D

-

W

R

R

E

P

I

I

G

G

P

K

Y

G

T

T

F

A

T

G

P

P

active site: F35 (= F41), G37 (= G43), K158 (= K159), E192 (= E193), L215 (= L216)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), K158 (= K159), Y163 (= Y164), E192 (= E193), G195 (= G196), E196 (≠ A197), N197 (= N198), L215 (= L216), G217 (= G218), I218 (= I219), T219 (= T220), G254 (= G255), T255 (= T256)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
37% identity, 91% coverage: 9:273/290 of query aligns to 3:269/297 of 2ej3A

query
sites
2ej3A

R

K

D

A

G

G

F

L

L

I

W

W

F

M

D

N

G

G

K

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

K

K

I

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

-

E

-

T

-

A

-

K

G

G

G

P

K

A

V

I

F

F

K

R

L

L

R

K

E

E

H

H

S

V

Q

K

R
|
R

L

F

I

Y

D

N

S

S

G

A

R

K

I

V

L

L

G

R

F

M

E

E

V

I

P

P

W

F

T

A

V

P

E

E

I

L

D

E

E

E

A

A

T

I

M

K

A

E

T

V

V

V

K

R

A

R

N

N

N

G

I

Y

V

R

D

S

G

C

Y
|
Y

V

I

R
|
R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

-

L

Q

P

T

N

K

P

I

A

R

E

F

V

A

M

V

V

A

A

T

A

W

W

S

E

W

W

P

V

S

R

Y

-

W

-

S

-

P

-

E

-

A

-

R

-

M

-

K

K

G

G

I

A

R

R

L

L

N

I

I

T

S

S

T

S

W

W

K

A

R
|
R

P

F

H

P

P

A

E

N

T

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

M

L

S

A

K

K

H

M

K

E

A

A

E

V

G

A

D

A

G

G

Y

A

E

D

D

E

S

A

L

L

M

L

L

L

D

D

W

E

R

E

G

G

Q

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N

N

I

L

F

F

V

V

F

R

G

D

N

G

E

V

L

I

H

Y

T

A

P

L

T

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

A

R

L

I

A

A

K

K

K

D

R

L

G

G

I

Y

T

E

V

V

V

Q

E

V

R

V

A

R

I

A

F

T

P

R

E

D

E

Q

M

L

T

Y

K

M

A

A

S

D

E

E

C

V

F

F

L

M

T

T

G
|
G

T
|
T

A
|
A

E

E

V

V

T

T

P

P

V

V

-

S

-

M

-

I

-

D

-

W

R

R

E

P

I

I

G

G

P

K

Y

G

T

T

F

A

T

G

P

P

active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
binding [1-(aminomethyl)cyclohexyl]acetic acid: Y94 (= Y96), R96 (= R98), Y155 (= Y164), G187 (= G196), E188 (≠ A197), G246 (= G255), T247 (= T256), A248 (= A257), A249 (= A258)
binding pyridoxal-5'-phosphate: R58 (= R60), R139 (= R148), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (≠ A197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
37% identity, 91% coverage: 9:273/290 of query aligns to 3:269/297 of 2eiyA

query
sites
2eiyA

R

K

D

A

G

G

F

L

L

I

W

W

F

M

D

N

G

G

K

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

K

K

I

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

-

E

-

T

-

A

-

K

G

G

G

P

K

A

V

I

F

F

K

R

L

L

R

K

E

E

H

H

S

V

Q

K

R
|
R

L

F

I

Y

D

N

S

S

G

A

R

K

I

V

L

L

G

R

F

M

E

E

V

I

P

P

W

F

T

A

V

P

E

E

I

L

D

E

E

E

A

A

T

I

M

K

A

E

T

V

V

V

K

R

A

R

N

N

N

G

I

Y

V

R

D

S

G

C

Y
|
Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

-

L

Q

P

T

N

K

P

I

A

R

E

F

V

A

M

V

V

A

A

T

A

W

W

S

E

W

W

P

V

S

R

Y

-

W

-

S

-

P

-

E

-

A

-

R

-

M

-

K

K

G

G

I

A

R

R

L

L

N

I

I

T

S

S

T

S

W

W

K

A

R
|
R

P

F

H

P

P

A

E

N

T

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

M

L

S

A

K

K

H

M

K

E

A

A

E

V

G

A

D

A

G

G

Y

A

E

D

D

E

S

A

L

L

M

L

L

L

D

D

W

E

R

E

G

G

Q

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N
|
N

I

L

F

F

V

V

F

R

G

D

N

G

E

V

L

I

H

Y

T

A

P

L

T

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

A

R

L

I

A

A

K

K

K

D

R

L

G

G

I

Y

T

E

V

V

V

Q

E

V

R

V

A

R

I

A

F

T

P

R

E

D

E

Q

M

L

T

Y

K

M

A

A

S

D

E

E

C

V

F

F

L

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

-

S

-

M

-

I

-

D

-

W

R

R

E

P

I

I

G

G

P

K

Y

G

T

T

F

A

T

G

P

P

active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
binding 4-methyl valeric acid: F35 (= F41), Y94 (= Y96), Y155 (= Y164), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R58 (= R60), R139 (= R148), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (≠ A197), N189 (= N198), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
37% identity, 91% coverage: 9:273/290 of query aligns to 3:269/297 of 1wrvA

query
sites
1wrvA

R

K

D

A

G

G

F

L

L

I

W

W

F

M

D

N

G

G

K

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

K

K

I

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

-

E

-

T

-

A

-

K

G

G

G

P

K

A

V

I

F

F

K

R

L

L

R

K

E

E

H

H

S

V

Q

K

R
|
R

L

F

I

Y

D

N

S

S

G

A

R

K

I

V

L

L

G

R

F

M

E

E

V

I

P

P

W

F

T

A

V

P

E

E

I

L

D

E

E

E

A

A

T

I

M

K

A

E

T

V

V

V

K

R

A

R

N

N

N

G

I

Y

V

R

D

S

G

C

Y

Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

-

L

Q

P

T

N

K

P

I

A

R

E

F

V

A

M

V

V

A

A

T

A

W

W

S

E

W

W

P

V

S

R

Y

-

W

-

S

-

P

-

E

-

A

-

R

-

M

-

K

K

G

G

I

A

R

R

L

L

N

I

I

T

S

S

T

S

W

W

K

A

R
|
R

P

F

H

P

P

A

E

N

T

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

M

L

S

A

K

K

H

M

K

E

A

A

E

V

G

A

D

A

G

G

Y

A

E

D

D

E

S

A

L

L

M

L

L

L

D

D

W

E

R

E

G

G

Q

Y

V

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N
|
N

I

L

F

F

V

V

F

R

G

D

N

G

E

V

L

I

H

Y

T

A

P

L

T

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

A

R

L

I

A

A

K

K

K

D

R

L

G

G

I

Y

T

E

V

V

V

Q

E

V

R

V

A

R

I

A

F

T

P

R

E

D

E

Q

M

L

T

Y

K

M

A

A

S

D

E

E

C

V

F

F

L

M

T

T

G
|
G

T
|
T

A

A

E

E

V

V

T

T

P

P

V

V

-

S

-

M

-

I

-

D

-

W

R

R

E

P

I

I

G

G

P

K

Y

G

T

T

F

A

T

G

P

P

active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
binding pyridoxal-5'-phosphate: R58 (= R60), R139 (= R148), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (≠ A197), N189 (= N198), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
37% identity, 91% coverage: 9:273/290 of query aligns to 3:266/294 of 2ej2A

query
sites
2ej2A

R

K

D

A

G

G

F

L

L

I

W

W

F

M

D

N

G

G

K

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

K

K

I

T

H

S

V

V

L

L

T

S

H

H

G

A

L

L

H

H

Y

Y

G

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

-

E

-

T

-

A

-

K

G

G

G

P

K

A

V

I

F

F

K

R

L

L

R

K

E

E

H

H

S

V

Q

K

R
|
R

L

F

I

Y

D

N

S

S

G

A

R

K

I

V

L

L

G

R

F

M

E

E

V

I

P

P

W

F

T

A

V

P

E

E

I

L

D

E

E

E

A

A

T

I

M

K

A

E

T

V

V

V

K

R

A

R

N

N

N

G

I

Y

V

R

D

S

G

C

Y
|
Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

-

L

Q

P

T

N

K

P

I

A

R

E

F

V

A

M

V

V

A

A

T

A

W

W

S

E

W

W

P

-

S

-

Y

-

W

-

S

-

P

-

E

-

A

-

R

-

M

-

K

-

G

G

I

A

R

R

L

L

N

I

I

T

S

S

T

S

W

W

K

A

R
|
R

P

F

H

P

P

A

E

N

T

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

M

L

S

A

K

K

H

M

K

E

A

A

E

V

G

A

D

A

G

G

Y

A

E

D

D

E

S

A

L

L

M

L

L

L

D

D

W

E

R

E

G

G

Q

Y

V

V

A

A

E
|
E

A

G

T
x
S

G
|
G

A
x
E

N
|
N

I

L

F

F

V

V

F

R

G

D

N

G

E

V

L

I

H

Y

T

A

P

L

T

E

P

H

D

S

C

V

F

N

L
|
L

N

E

G
|
G

I
|
I

T
|
T

R

R

Q

D

T

S

V

V

I

I

A

R

L

I

A

A

K

K

K

D

R

L

G

G

I

Y

T

E

V

V

V

Q

E

V

R

V

A

R

I

A

F

T

P

R

E

D

E

Q

M

L

T

Y

K

M

A

A

S

D

E

E

C

V

F

F

L

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

-

S

-

M

-

I

-

D

-

W

R

R

E

P

I

I

G

G

P

K

Y

G

T

T

F

A

T

G

P

P

active site: F35 (= F41), G37 (= G43), K147 (= K159), E181 (= E193), L204 (= L216)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: G37 (= G43), R58 (= R60), Y94 (= Y96), R136 (= R148), K147 (= K159), Y152 (= Y164), E181 (= E193), S183 (≠ T195), G184 (= G196), E185 (≠ A197), N186 (= N198), L204 (= L216), G206 (= G218), I207 (= I219), T208 (= T220), G243 (= G255), T244 (= T256), A245 (= A257)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
34% identity, 96% coverage: 12:290/290 of query aligns to 3:283/290 of 5mr0D

query
sites
5mr0D

F

Y

L

V

W

Y

F

M

D

D

G

G

K

E

L

F

V

V

P

P

W

E

R

N

D

E

A

A

K

K

I

V

H

S

V

I

L

F

T

D

H

H

G

G

L

F

H

L

Y

Y

G

G

S

D

C

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

G

N

G

G

K

R

V

V

F

F

K

R

L

L

R

K

E

E

H

H

S

I

Q

D

R
|
R

L

L

I

Y

D

D

S

S

G

A

R

K

I

A

L

I

G

D

F

L

E

E

V

I

P

P

W

I

T

T

V

K

E

E

I

F

D

M

E

E

A

I

T

I

M

L

A

E

T

T

V

L

K

R

A

K

N

N

I

L

V

R

D

D

G

A

Y

Y

V

I

R
|
R

P

P

V

I

A

V

W

T

R

R

G

G

S

I

E

G

M

D

M

L

G
|
G

V
x
L

A

D

A

P

Q

R

T

K

T

C

K

Q

I

N

-

P

R

S

F

I

A

I

V

V

A

I

T

T

W

K

S

P

W

W

P

G

S

K

Y

L

W

Y

S

G

P

-

E

D

A

L

R

Y

M

E

K

K

G

G

I

L

R

T

L

A

N

I

I

T

S

V

T

A

W

V

K

R

R

R

P

N

H

S

P

F

E

D

T

A

A

L

P

P

T

P

A

N

S

I

K
|
K

A

S

A

L

G

N

L

-

Y
|
Y

M

L

I

N

C

N

T

I

M

L

S

A

K

K

H

I

K

E

A

A

E

N

G

A

D

K

G

G

Y

G

E

D

D

E

S

A

L

I

M

F

L

L

D

D

W

R

R

N

G

G

Q

Y

V

V

A

S

E
|
E

A

G

T

S

G
|
G

A
x
D

N
|
N

I

I

F

F

F

V

V

V

F

K

G

N

N

G

E

A

L

I

H

T

T

T

P

P

T

P

P

T

D

I

C

N

F

N

L
|
L

N

R

G
|
G

I
|
I

T
|
T

R

R

Q

E

T

A

V

V

I

I

A

E

L

I

A

I

K

N

K

R

R

L

G

G

I

I

T

P

V

F

V

K

E

E

R

T

A

N

I

I

F

G

P

L

E

Y

E

D

M

L

T

Y

K

T

A

A

S

D

E

E

C

V

F

F

L

V

T

T

G
|
G

T
|
T

A

A

E

E

V

I

T

A

P

P

V

I

-

V

-

I

-

D

-

G

R

R

E

K

I

I

G

G

P

-

Y

-

T

D

F

G

T

K

P

P

G

G

D

E

I

I

T

T

R

R

Q

K

L

L

I

M

T

E

D

E

Y

F

D

S

A

K

L

L

V

T

R

E

S

S

active site: F32 (= F41), G34 (= G43), K150 (= K159), E183 (= E193), L206 (= L216)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: F32 (= F41), R51 (= R60), R89 (= R98), G100 (= G109), L101 (≠ V110), K150 (= K159), Y154 (= Y164), E183 (= E193), G186 (= G196), D187 (≠ A197), N188 (= N198), L206 (= L216), G208 (= G218), I209 (= I219), T210 (= T220), G245 (= G255), T246 (= T256)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
34% identity, 94% coverage: 12:284/290 of query aligns to 4:278/290 of 5e25A

query
sites
5e25A

F

L

L

V

W

Y

F

M

D

N

G

G

K

E

L

F

V

V

P

P

W

E

R

S

D

Q

A

A

K

K

I

V

H

S

V

V

L

F

T

D

H

H

G

G

L

F

H

L

Y

Y

G

G

S

D

C

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

G

N

G

G

K

K

V

V

F

F

K

K

L

L

R

Y

E

E

H

H

S

I

Q

D

R
|
R

L

L

I

Y

D

D

S

C

G

A

R

R

I

V

L

I

G

D

F

L

E

K

V

I

P

P

W

L

T

S

V

K

E

E

I

F

D

A

E

E

A

A

T

I

M

L

A

E

T

T

V

L

K

R

A

R

N

N

I

L

V

R

D

D

G

A

Y
|
Y

V

I

R
|
R

P

P

V

I

A

V

W

T

R

R

G

G

S

A

E

G

M

D

M

L

G

G

V

L

A

D

A

P

Q

R

T

K

T

C

-

P

K

S

I

P

R

N

F

V

A

I

V

I

A

I

T

T

W

K

S

P

W

W

P

G

S

K

Y

L

W

Y

S

G

P

-

E

D

A

L

R

Y

M

E

K

K

G

G

I

L

R

K

L

A

N

I

I

T

S

V

T

A

W

I

K

R

R

R

P

N

H

A

P

I

E

D

T

S

A

L

P

P

T

P

A

N

S

I

K
|
K

A

S

A

L

G

N

L

-

Y
|
Y

M

L

I

N

C

N

T

I

M

L

S

A

K

K

H

I

K

E

A

A

E

N

G

A

D

K

G

G

Y

G

E

D

D

E

S

A

L

I

M

F

L

L

D

D

W

H

R

N

G

G

Q

Y

V

I

A

S

E
|
E

A

G

T

S

G
|
G

A
x
D

N
|
N

I

I

F

F

F

I

V

V

F

K

G

N

N

G

E

T

L

I

H

T

T

T

P

P

T

P

P

T

D

L

C

N

F

N

L
|
L

N

K

G
|
G

I
|
I

T
|
T

R

R

Q

Q

T

V

V

V

I

I

A

E

L

L

A

I

K

N

K

E

R

L

G

E

I

I

T

P

V

F

V

R

E

E

R

A

A

N

I

I

F

G

P

L

E

F

E

D

M

L

T

Y

K

S

A

A

S

D

E

E

C

I

F

F

L

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

A

P

P

V

V

R

T

E

Y

I

I

G

D

P

G

Y

R

T

T

F

V

-

G

-

N

-

G

T

K

P

P

G

G

D

K

I

V

T

T

R

K

Q

M

L

L

I

M

T

E

D

K

Y

F

active site: F33 (= F41), G35 (= G43), K151 (= K159), E184 (= E193), L207 (= L216)
binding 2-oxoglutaric acid: F33 (= F41), G35 (= G43), Y88 (= Y96), R90 (= R98), K151 (= K159), G187 (= G196), G246 (= G255), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), D188 (≠ A197), N189 (= N198), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
33% identity, 96% coverage: 12:288/290 of query aligns to 5:290/307 of 6q8eA

query
sites
6q8eA

F

Y

L

L

W

W

F

W

D

N

G

H

K

R

L

I

V

I

P

P

W

W

R

E

D

E

A

A

K

T

I

V

H

H

V

L

L

T

T

D

H

Y

G

W

L

W

H

A

Y

S

G

V

S

T

C

A

V

V

F
|
F

E

E

G

G

E

I

R

R

V

G

Y

Y

G

W

G

N

K

N

-

A

-

E

-

G

-

E

-

M

-

Y

V

I

F

F

K

R

L

L

R

E

E

D

H

H

S

A

Q

R

R
|
R

L

L

I

E

D

Q

S

S

G

M

R

Q

I

L

L

I

G

R

F

M

E

P

V

K

P

E

W

F

T

T

V

V

E

D

E

E

I

I

D

C

E

Q

A

A

T

T

M

I

A

D

T

L

V

V

K

R

A

A

N

N

N

D

I

Y

V

R

-

G

D

D

G

V

Y

Y

V

I

R

M

P

P

V

L

A

A

W

Y

R

A

-

V

G

G

S

N

E

K

M

A

M

F

G

S

V

V

A

V

A

G

Q

D

T

R

T

T

K

T

I

E

R

M

F

F

A

-

V

-

A

-

T

I

W

Y

S

S

W

R

P

P

S

A

Y

V

W

S

S

R

P

L

E

E

A

E

R

D

M

F

K

-

G

S

I

L

R

H

L

A

N

C

I

Y

S

S

T

S

W

W

K

T

R

R

P

I

H

N

P

E

E

R

T

V

A

L

P

P

T

P

A

R

S

I

K
|
K

A

A

A

L

G

A

L

N

Y
|
Y

M

R

I

N

C

S

T

Q

M

L

S

A

K

S

H

S

K

E

A

A

E

A

G

M

D

N

G

G

Y

Y

E

D

D

T

S

A

L

L

M

F

L

L

D

N

W

P

R

E

G

G

Q

K

V

V

A

A

E
|
E

A

G

T
|
T

G
|
G

A
x
S

N
x
C

I

V

F

F

V

V

F

R

G

K

N

G

E

K

L

L

H

I

T

T

P

P

-

D

T

I

P

T

D

S

C

G

F

I

L
|
L

N

E

G
x
S

I
|
I

T
|
T

R

R

Q

D

T

T

V

V

I

I

A

H

L

L

A

A

K

R

K

E

-

V

R

L

G

G

I

L

T

E

V

V

V

E

E

E

R

R

A

V

I

V

F

D

P

R

E

T

E

E

M

T

T

Y

K

L

A

A

S

D

E

E

C

A

F

F

L

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

V

I

R

A

E

S

I

I

G

D

P

R

Y

H

T

E

F

M

-

K

-

H

-

G

T

A

P

P

G

G

D

P

I

I

T

T

R

R

Q

Q

L

L

I

R

T

D

D

I

Y

Y

D

R

A

E

L

V

V

V

active site: F34 (= F41), K156 (= K159), E190 (= E193), L214 (= L216)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), T192 (= T195), G193 (= G196), S194 (≠ A197), C195 (≠ N198), L214 (= L216), S216 (≠ G218), I217 (= I219), T218 (= T220), G254 (= G255), T255 (= T256)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
32% identity, 96% coverage: 12:288/290 of query aligns to 5:290/309 of 7neaA

query
sites
7neaA

F

Y

L

L

W

W

F

W

D

N

G

H

K

R

L

I

V

I

P

P

W

W

R

E

D

E

A

A

K

T

I

V

H

H

V

L

L

T

T

D

H

Y

G

W

L

W

H

A

Y

S

G

V

S

T

C

A

V

V

F
|
F

E

E

G

V

E

I

R

S

V

G

Y

Y

G

W

G

N

K

N

-

A

-

E

-

G

-

E

-

M

-

Y

V

I

F

F

K

R

L

L

R

E

E

D

H

H

S

A

Q

R

R
|
R

L

L

I

E

D

Q

S

S

G

M

R

Q

I

L

L

I

G

R

F

M

E

P

V

K

P

E

W

F

T

T

V

V

E

D

E

E

I

I

D

C

E

Q

A

A

T

T

M

I

A

D

T

L

V

V

K

R

A

A

N

N

N

D

I

Y

V

R

-

G

D

D

G

V

Y

F

V

I

R

M

P

P

V

L

A

A

W

Y

R

A

-

V

G

G

S

N

E

K

M

A

M

F

G

S

V

V

A

V

A

G

Q

D

T

R

T

T

K

T

I

E

R

M

F

F

A

-

V

-

A

-

T

I

W

Y

S

S

W

R

P

P

S

A

Y

V

W

S

S

R

P

L

E

E

A

E

R

D

M

F

K

-

G

S

I

L

R

H

L

A

N

C

I

Y

S

S

T

S

W

W

K

T

R

R

P

I

H

N

P

E

E

R

T

V

A

L

P

P

T

P

A

R

S

I

K
|
K

A

A

A

L

G

A

L

N

Y
|
Y

M

R

I

N

C

S

T

Q

M

L

S

A

K

S

H

S

K

E

A

A

E

A

G

M

D

N

G

G

Y

Y

E

D

D

T

S

A

L

L

M

F

L

L

D

N

W

P

R

E

G

G

Q

K

V

V

A

A

E
|
E

A

G

T

T

G
|
G

A
x
S

N
x
C

I

V

F

F

V

V

F

R

G

K

N

G

E

K

L

L

H

I

T

T

P

P

-

D

T

I

P

T

D

S

C

G

F

I

L
|
L

N

E

G
x
S

I
|
I

T
|
T

R

R

Q

D

T

T

V

V

I

I

A

H

L

L

A

A

K

R

K

E

-

V

R

L

G

G

I

L

T

E

V

V

V

E

E

E

R

R

A

V

I

V

F

D

P

R

E

T

E

E

M

T

T

Y

K

L

A

A

S

D

E

E

C

A

F

F

L

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

V

I

R

A

E

S

I

I

G

D

P

R

Y

H

T

E

F

M

-

K

-

H

-

G

T

A

P

P

G

G

D

P

I

I

T

T

R

R

Q

Q

L

L

I

R

T

D

D

I

Y

Y

D

R

A

E

L

V

V

V

active site: F34 (= F41), K