SitesBLAST
Comparing WP_043743836.1 NCBI__GCF_000009985.1:WP_043743836.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2f2aA Structure of tRNA-dependent amidotransferase gatcab complexed with gln (see paper)
52% identity, 96% coverage: 16:483/490 of query aligns to 25:478/485 of 2f2aA
- active site: K79 (= K78), S154 (= S159), S155 (= S160), S173 (≠ T178), T175 (= T180), G176 (= G181), G177 (= G182), S178 (= S183), Q181 (= Q186)
- binding glutamine: G130 (= G129), S154 (= S159), D174 (= D179), T175 (= T180), G176 (= G181), S178 (= S183), F206 (= F211), Y309 (= Y314), Y310 (= Y315), R358 (= R362), D425 (= D429)
2dqnA Structure of tRNA-dependent amidotransferase gatcab complexed with asn (see paper)
52% identity, 96% coverage: 16:483/490 of query aligns to 25:478/485 of 2dqnA
- active site: K79 (= K78), S154 (= S159), S155 (= S160), S173 (≠ T178), T175 (= T180), G176 (= G181), G177 (= G182), S178 (= S183), Q181 (= Q186)
- binding asparagine: M129 (= M128), G130 (= G129), T175 (= T180), G176 (= G181), S178 (= S183), Y309 (= Y314), Y310 (= Y315), R358 (= R362), D425 (= D429)
3h0mA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
48% identity, 96% coverage: 8:478/490 of query aligns to 6:466/478 of 3h0mA
- active site: K72 (= K78), S147 (= S159), S148 (= S160), S166 (≠ T178), T168 (= T180), G169 (= G181), G170 (= G182), S171 (= S183), Q174 (= Q186)
- binding glutamine: M122 (= M128), G123 (= G129), D167 (= D179), T168 (= T180), G169 (= G181), G170 (= G182), S171 (= S183), F199 (= F211), Y302 (= Y314), R351 (= R362), D418 (= D429)
3h0lA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
48% identity, 96% coverage: 8:478/490 of query aligns to 6:466/478 of 3h0lA
- active site: K72 (= K78), S147 (= S159), S148 (= S160), S166 (≠ T178), T168 (= T180), G169 (= G181), G170 (= G182), S171 (= S183), Q174 (= Q186)
- binding asparagine: G123 (= G129), S147 (= S159), G169 (= G181), G170 (= G182), S171 (= S183), Y302 (= Y314), R351 (= R362), D418 (= D429)
3kfuE Crystal structure of the transamidosome (see paper)
46% identity, 96% coverage: 11:480/490 of query aligns to 4:456/468 of 3kfuE
4n0iA Crystal structure of s. Cerevisiae mitochondrial gatfab in complex with glutamine (see paper)
37% identity, 82% coverage: 69:471/490 of query aligns to 29:443/450 of 4n0iA
- active site: K38 (= K78), S116 (= S159), S117 (= S160), T135 (= T178), T137 (= T180), G138 (= G181), G139 (= G182), S140 (= S183), L143 (≠ Q186)
- binding glutamine: G89 (= G129), T137 (= T180), G138 (= G181), S140 (= S183), Y168 (≠ F211), Y271 (= Y314), Y272 (= Y315), R320 (= R362), D404 (= D429)
Q7XJJ7 Fatty acid amide hydrolase; AtFAAH; N-acylethanolamine amidohydrolase; EC 3.5.1.99 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 89% coverage: 51:487/490 of query aligns to 177:597/607 of Q7XJJ7
- K205 (= K78) mutation to A: Loss of activity.
- SS 281:282 (= SS 159:160) mutation to AA: Loss of activity.
- GGGS 302:305 (≠ TGGS 180:183) binding
- S305 (= S183) mutation to A: Loss of activity.
- R307 (= R185) mutation to A: Loss of activity.
- S360 (≠ H238) mutation to A: No effect.
6diiH Structure of arabidopsis fatty acid amide hydrolase in complex with methyl linolenyl fluorophosphonate (see paper)
31% identity, 89% coverage: 51:487/490 of query aligns to 177:597/616 of 6diiH
- binding methyl-9Z,12Z,15Z-octadecatrienylphosphonofluoridate: G255 (≠ A127), T258 (≠ S130), S281 (= S159), G302 (≠ T180), G303 (= G181), S305 (= S183), S472 (≠ T367), I532 (≠ D421), M539 (≠ N428)
Sites not aligning to the query:
3a1iA Crystal structure of rhodococcus sp. N-771 amidase complexed with benzamide (see paper)
34% identity, 84% coverage: 68:478/490 of query aligns to 85:497/508 of 3a1iA
- active site: K95 (= K78), S170 (= S159), S171 (= S160), G189 (≠ T178), Q191 (≠ T180), G192 (= G181), G193 (= G182), A194 (≠ S183), I197 (≠ Q186)
- binding benzamide: F145 (≠ M128), S146 (≠ G129), G147 (≠ S130), Q191 (≠ T180), G192 (= G181), G193 (= G182), A194 (≠ S183), W327 (≠ Y314)
6c6gA An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme. Inhibitor bound complex. (see paper)
31% identity, 96% coverage: 7:478/490 of query aligns to 1:448/457 of 6c6gA