SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043744492.1 NCBI__GCF_000009985.1:WP_043744492.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q07607 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; Protein MosA; EC 4.3.3.7 from Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
56% identity, 100% coverage: 1:290/290 of query aligns to 1:291/292 of Q07607

query
sites
Q07607

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K161 (= K160) active site, Schiff-base intermediate with substrate

Q8UGL3 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
59% identity, 100% coverage: 1:290/290 of query aligns to 1:293/294 of Q8UGL3

query
sites
Q8UGL3

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-

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T45 (= T44) binding
P49 (= P48) L-lysine inhibitor binding; via carbonyl oxygen
H56 (= H55) L-lysine inhibitor binding
N80 (= N79) L-lysine inhibitor binding
E84 (= E83) L-lysine inhibitor binding
Y106 (= Y105) L-lysine inhibitor binding
K162 (= K160) active site, Schiff-base intermediate with substrate

4i7wA Agrobacterium tumefaciens dhdps with lysine and pyruvate
58% identity, 100% coverage: 1:290/290 of query aligns to 1:293/294 of 4i7wA

query
sites
4i7wA

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active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K162 (= K160), I204 (= I202)
binding lysine: S48 (= S47), P49 (= P48), L51 (= L50), S52 (= S51), H53 (= H52), H56 (= H55), N80 (= N79), E84 (= E83), Y106 (= Y105)

7mjfA Crystal structure of candidatus liberibacter solanacearum dihydrodipicolinate synthase with pyruvate and succinic semi-aldehyde bound in active site
49% identity, 100% coverage: 1:289/290 of query aligns to 1:293/296 of 7mjfA

query
sites
7mjfA

M

M

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x
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binding (4R)-4-oxidanyl-2-oxidanylidene-heptanedioic acid: A8 (= A8), G43 (= G42), T44 (= T43), T45 (= T44), Y133 (= Y132), N135 (≠ I134), R138 (= R137), K162 (= K160), G187 (= G185), I204 (= I202), V206 (= V204)
binding (4S)-4-hydroxy-2-oxoheptanedioic acid: A8 (= A8), G43 (= G42), T44 (= T43), T45 (= T44), Y133 (= Y132), N135 (≠ I134), R138 (= R137), K162 (= K160), G187 (= G185), I204 (= I202)

7lvlA Dihydrodipicolinate synthase bound with allosteric inhibitor (s)- lysine from candidatus liberibacter solanacearum
49% identity, 100% coverage: 1:289/290 of query aligns to 1:293/296 of 7lvlA

query
sites
7lvlA

M

M

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S

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A

A

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N

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D

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G

G

T

S

H

S

A

G

V

L

V

V

P

P

C

A

G

G

T
|
T

T

T

G

G

E

E

S
|
S

P
x
S

T

T

L
|
L

S
|
S

H

Y

D

E

E

E

H
|
H

H

C

R

R

V

V

E

E

L

L

C

C

L

V

E

K

V

T

A

A

R

A

G

G

K

R

V

V

P

P

V

V

I

M

A

A

G

G

T

A

G

G

S

S

N
|
N

S

N

T

T

D

K

E
|
E

A

S

V

I

A

E

L

L

T

A

Q

Q

H

Y

A

A

R

Q

K

N

A

T

G

G

A

A

D

D

A

A

A

L

L

L

V

V

A

V

P

P

Y
|
Y

N

N

K

K

P

P

S

N

Q

K

E

K

G

G

L

L

F

L

R

A

H

H

F

F

E

G

A

S

I

I

A

A

K

N

S

A

V

V

D

S

I

L

P

P

I

I

I

Y

V

I

Y
|
Y

N

N

I

N

P

P

G

S

R
|
R

S

T

V

V

I

I

D

E

I

M

S

D

V

V

E

D

T

T

F

M

V

A

R

E

L

L

-

V

S

K

A

T

L

Y

P

S

N

N

I

I

A

V

G

G

I

V

K
|
K

D

D

A

A

T

T

A

G

D

R

L

I

A

E

R

L

P

A

L

S

R

G

I

Q

R

R

A

I

A

A

L

C

D

G

E

S

R

D

L

F

C

I

Q

Q

L

L

S

S

G

G

E

D

D

D

A

S

T

S

A

A

I

L

A

G

F

F

N

N

A

V

Q

H

G

G

V

V

G

G

C

C

I
|
I

S

S

V

V

T

T

S

A

N

N

I

V

A

A

P

P

K

R

L

I

C

C

A

A

R

E

M

F

Q

Q

N

K

A

A

W

I

A

S

A

E

G

G

D

D

L

Y

A

R

T

Q

C

A

N

L

E

E

L

Y

N

Q

K

D

I

K

L

L

M

F

P

P

L

L

H

H

D

Q

A

A

L

L

F

F

C

I

E

E

T

P

S

S

P

I

A

S

P

S

V

V

K

K

Y

Y

A

A

A

L

S

S

L

R

L

L

G

G

K

R

S

N

A

V

P

S

-

L

D

V

V

V

R

R

L

A

P

P

L

M

V

V

P

S

A

I

S

L

E

E

N

K

-

E

A

T

R

M

H

F

R

A

V

I

E

D

A

Q

A

A

M

L

K

D

A

H

A

I

G

G

L

L

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K162 (= K160), I204 (= I202)
binding lysine: S48 (= S47), S49 (≠ P48), L51 (= L50), S52 (= S51), H56 (= H55), N80 (= N79), E84 (= E83), Y106 (= Y105)

4dxvA Crystal structure of dihydrodipicolinate synthase from acinetobacter baumannii complexed with mg and cl ions at 1.80 a resolution
47% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 4dxvA

query
sites
4dxvA

F

I

K

Q

G

G

S

S

I

I

T

V

A

A

L

I

I

V

T

T

P

P

-

M

F

L

R

K

N

D

G

G

K

G

V

V

D

D

E

W

K

K

A

S

F

L

Q

E

D

K

L

L

V

V

A

E

W

W

Q

H

I

I

A

E

E

Q

G

G

T

T

H

N

A

S

V

I

V

V

P

A

C

V

G

G

T
|
T

T

T

G

G

E

E

S

A

P

S

T

T

L

L

S

S

H

M

D

E

E

E

H

H

H

T

R

Q

V

V

V

I

E

K

L

E

C

I

L

I

E

R

V

V

A

A

R

N

G

K

K

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

D

R

E

E

A

A

V

I

A

E

L

L

T

T

Q

K

H

A

A

A

R

K

K

D

A

L

G

G

A

A

D

D

A

A

L

L

V

L

V

V

A
x
T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

Y

E

K

A

A

I

I

A

A

K

E

S

A

V

V

D

E

I

L

P

P

I

L

I

I

V

L

Y
|
Y

N
|
N

I
x
V

P
|
P

G

G

R
|
R

S
x
T

V

G

I

V

D

D

I

L

S

S

V

N

E

D

T

T

F

A

V

V

R

R

L

L

S

A

A

E

L

I

P

P

N

N

I

I

A

V

G

G

I

I

K
|
K

D

D

A

A

T

T

A

G

D

D

L

V

A

P

R

R

P

G

L

K

R

A

I

L

R

I

A

D

A

A

L

L

D

N

E

G

R

K

L

M

C

A

Q

V

L

Y

S

S

G

G

E

D

D

D

A

E

T

T

A

A

I

W

A

E

F

L

N

M

A

L

Q

L

G

G

G

A

V

D

G

G

C

N

I
|
I

S

S

V

V

T

T

S

A

N

N

I

I

A

A

P

P

K

K

L

A

C

M

A

S

R

E

M

V

Q

C

N

A

A

V

W

A

A

I

A

A

G

K

D

D

L

E

A

Q

T

Q

C

A

N

K

E

T

L

L

N

N

K

N

I

K

L

I

M

A

P

N

L

L

H

H

D

N

A

I

L

L

F

F

C

C

E

E

T

S

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

L

S

H

L

E

L

M

G

G

K

L

S

I

A

D

P

T

D

G

V

I

R

R

L

L

P

P

L

L

V

T

P

P

A

L

S

A

E

E

N

Q

A

Y

R

R

H

E

R

P

V

L

E

R

A

N

A

A

M

L

K

K

A

D

A

A

G

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding magnesium ion: T104 (≠ A103), N134 (= N133), V135 (≠ I134), P136 (= P135), T139 (≠ S138)

3u8gA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with oxalic acid at 1.80 a resolution
47% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3u8gA

query
sites
3u8gA

F

I

K

Q

G

G

S

S

I

I

T

V

A
|
A

L

I

I

V

T

T

P

P

-

M

F

L

R

K

N

D

G

G

K

G

V

V

D

D

E

W

K

K

A

S

F

L

Q

E

D

K

L

L

V

V

A

E

W

W

Q

H

I

I

A

E

E

Q

G

G

T

T

H

N

A

S

V

I

V
|
V

P

A

C

V

G
|
G

T
|
T

G

G

E

E

S

A

P

S

T

T

L

L

S

S

H

M

D

E

E

E

H

H

H

T

R

Q

V

V

V

I

E

K

L

E

C

I

L

I

E

R

V

V

A

A

R

N

G

K

K

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

D

R

E

E

A

A

V

I

A

E

L

L

T

T

Q

K

H

A

A

A

R

K

K

D

A

L

G

G

A

A

D

D

A

A

L
|
L

V

L

V

V

A

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

Y

E

K

A

A

I

I

A

A

K

E

S

A

V

V

D

E

I

L

P

P

I

L

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

S

T

V

G

I

V

D

D

I

L

S

S

V

N

E

D

T

T

F

A

V

V

R

R

L

L

S

A

A

E

L

I

P

P

N

N

I

I

A

V

G

G

I

I

K
|
K

D

D

A

A

T

T

A

G

D

D

L

V

A

P

R

R

P

G

L

K

R

A

I

L

R

I

A

D

A

A

L

L

D

N

E

G

R

K

L

M

C

A

Q

V

L

Y

S

S

G

G

E

D

D

D

A

E

T

T

A

A

I

W

A

E

F

L

N

M

A

L

Q

L

G

G

G

A

V

D

G

G

C

N

I
|
I

S

S

V

V

T

T

S

A

N

N

I

I

A

A

P

P

K

K

L

A

C

M

A

S

R

E

M

V

Q

C

N

A

A

V

W

A

A

I

A

A

G

K

D

D

L

E

A

Q

T

Q

C

A

N

K

E

T

L

L

N

N

K

N

I

K

L

I

M

A

P

N

L

L

H

H

D

N

A

I

L

L

F

F

C

C

E

E

T

S

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

L

S

H

L

E

L

M

G

G

K

L

S

I

A

D

P

T

D

G

V

I

R

R

L

L

P

P

L

L

V

T

P

P

A

L

S

A

E

E

N

Q

A

Y

R

R

H

E

R

P

V

L

E

R

A

N

A

A

M

L

K

K

A

D

A

A

G

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding oxalate ion: A8 (= A8), V40 (= V39), G43 (= G42), T44 (= T43), T45 (= T44), L101 (= L100), Y133 (= Y132), K161 (= K160)

3tdfA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 2-ketobutanoic acid at 1.99 a resolution
47% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3tdfA

query
sites
3tdfA

F

I

K

Q

G

G

S

S

I

I

T

V

A
|
A

L

I

I

V

T

T

P

P

-

M

F

L

R

K

N

D

G

G

K

G

V

V

D

D

E

W

K

K

A

S

F

L

Q

E

D

K

L

L

V

V

A

E

W

W

Q

H

I

I

A

E

E

Q

G

G

T

T

H

N

A

S

V

I

V

V

P

A

C

V

G
|
G

T
|
T

G

G

E

E

S

A

P

S

T

T

L

L

S

S

H

M

D

E

E

E

H

H

H

T

R

Q

V

V

V

I

E

K

L

E

C

I

L

I

E

R

V

V

A

A

R

N

G

K

K

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

D

R

E

E

A

A

V

I

A

E

L

L

T

T

Q

K

H

A

A

A

R

K

K

D

A

L

G

G

A

A

D

D

A

A

L

L

V

L

V

V

A

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

Y

E

K

A

A

I

I

A

A

K

E

S

A

V

V

D

E

I

L

P

P

I

L

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

S

T

V

G

I

V

D

D

I

L

S

S

V

N

E

D

T

T

F

A

V

V

R

R

L

L

S

A

A

E

L

I

P

P

N

N

I

I

A

V

G

G

I

I

K
|
K

D

D

A

A

T

T

A

G

D

D

L

V

A

P

R

R

P

G

L

K

R

A

I

L

R

I

A

D

A

A

L

L

D

N

E

G

R

K

L

M

C

A

Q

V

L

Y

S

S

G

G

E

D

D

D

A

E

T

T

A

A

I

W

A

E

F

L

N

M

A

L

Q

L

G

G

G

A

V

D

G

G

C

N

I
|
I

S

S

V

V

T

T

S

A

N

N

I

I

A

A

P

P

K

K

L

A

C

M

A

S

R

E

M

V

Q

C

N

A

A

V

W

A

A

I

A

A

G

K

D

D

L

E

A

Q

T

Q

C

A

N

K

E

T

L

L

N

N

K

N

I

K

L

I

M

A

P

N

L

L

H

H

D

N

A

I

L

L

F

F

C

C

E

E

T

S

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

L

S

H

L

E

L

M

G

G

K

L

S

I

A

D

P

T

D

G

V

I

R

R

L

L

P

P

L

L

V

T

P

P

A

L

S

A

E

E

N

Q

A

Y

R

R

H

E

R

P

V

L

E

R

A

N

A

A

M

L

K

K

A

D

A

A

G

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding 2-ketobutyric acid: A8 (= A8), G43 (= G42), T44 (= T43), T45 (= T44), Y133 (= Y132), K161 (= K160), I203 (= I202)

3tceA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 5-hydroxylysine at 2.6 a resolution
47% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3tceA

query
sites
3tceA

F

I

K

Q

G

G

S

S

I

I

T

V

A

A

L

I

I

V

T

T

P

P

-

M

F

L

R

K

N

D

G

G

K

G

V

V

D

D

E

W

K

K

A

S

F

L

Q

E

D

K

L

L

V

V

A

E

W

W

Q

H

I

I

A

E

E

Q

G

G

T

T

H

N

A

S

V

I

V

V

P

A

C

V

G

G

T
|
T

T

T

G

G

E

E

S
x
A

P
x
S

T

T

L
|
L

S
|
S

H
x
M

D

E

E

E

H
|
H

H

T

R

Q

V

V

V

I

E

K

L

E

C

I

L

I

E

R

V

V

A

A

R

N

G

K

K

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

D

R

E

E

A

A

V

I

A

E

L

L

T

T

Q

K

H

A

A

A

R

K

K

D

A

L

G

G

A

A

D

D

A

A

L

L

V

L

V

V

A

T

P

P

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

Y

E

K

A

A

I

I

A

A

K

E

S

A

V

V

D

E

I

L

P

P

I

L

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

S

T

V

G

I

V

D

D

I

L

S

S

V

N

E

D

T

T

F

A

V

V

R

R

L

L

S

A

A

E

L

I

P

P

N

N

I

I

A

V

G

G

I

I

K
|
K

D

D

A

A

T

T

A

G

D

D

L

V

A

P

R

R

P

G

L

K

R

A

I

L

R

I

A

D

A

A

L

L

D

N

E

G

R

K

L

M

C

A

Q

V

L

Y

S

S

G

G

E

D

D

D

A

E

T

T

A

A

I

W

A

E

F

L

N

M

A

L

Q

L

G

G

G

A

V

D

G

G

C

N

I
|
I

S

S

V

V

T

T

S

A

N

N

I

I

A

A

P

P

K

K

L

A

C

M

A

S

R

E

M

V

Q

C

N

A

A

V

W

A

A

I

A

A

G

K

D

D

L

E

A

Q

T

Q

C

A

N

K

E

T

L

L

N

N

K

N

I

K

L

I

M

A

P

N

L

L

H

H

D

N

A

I

L

L

F

F

C

C

E

E

T

S

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

L

S

H

L

E

L

M

G

G

K

L

S

I

A

D

P

T

D

G

V

I

R

R

L

L

P

P

L

L

V

T

P

P

A

L

S

A

E

E

N

Q

A

Y

R

R

H

E

R

P

V

L

E

R

A

N

A

A

M

L

K

K

A

D

A

A

G

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding 5-hydroxylysine: A48 (≠ S47), S49 (≠ P48), L51 (= L50), S52 (= S51), M53 (≠ H52), H56 (= H55), Y106 (= Y105)

3rk8A Crystal structure of the chloride inhibited dihydrodipicolinate synthase from acinetobacter baumannii complexed with pyruvate at 1.8 a resolution
47% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3rk8A

query
sites
3rk8A

F

I

K

Q

G

G

S

S

I

I

T

V

A

A

L

I

I

V

T

T

P

P

-

M

F

L

R

K

N

D

G

G

K

G

V

V

D

D

E

W

K

K

A

S

F

L

Q

E

D

K

L

L

V

V

A

E

W

W

Q

H

I

I

A

E

E

Q

G

G

T

T

H

N

A

S

V

I

V

V

P

A

C

V

G

G

T
|
T

T

T

G

G

E

E

S

A

P

S

T

T

L

L

S

S

H

M

D

E

E

E

H

H

H

T

R

Q

V

V

V

I

E

K

L

E

C

I

L

I

E

R

V

V

A

A

R

N

G

K

K

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

D

R

E

E

A

A

V

I

A

E

L

L

T

T

Q

K

H

A

A

A

R

K

K

D

A

L

G

G

A

A

D

D

A

A

L

L

V

L

V

V

A

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

Y

E

K

A

A

I

I

A

A

K

E

S

A

V

V

D

E

I

L

P

P

I

L

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

S

T

V

G

I

V

D

D

I

L

S

S

V

N

E

D

T

T

F

A

V

V

R

R

L

L

S

A

A

E

L

I

P

P

N

N

I

I

A

V

G

G

I

I

K
|
K

D

D

A

A

T

T

A

G

D

D

L

V

A

P

R

R

P

G

L

K

R

A

I

L

R

I

A

D

A

A

L

L

D

N

E

G

R

K

L

M

C

A

Q

V

L

Y

S

S

G
|
G

E

D

D

D

A

E

T

T

A

A

I

W

A

E

F

L

N

M

A

L

Q

L

G

G

G

A

V

D

G

G

C

N

I
|
I

S

S

V

V

T

T

S

A

N

N

I

I

A

A

P

P

K

K

L

A

C

M

A

S

R

E

M

V

Q

C

N

A

A

V

W

A

A

I

A

A

G

K

D

D

L

E

A

Q

T

Q

C

A

N

K

E

T

L

L

N

N

K

N

I

K

L

I

M

A

P

N

L

L

H

H

D

N

A

I

L

L

F
|
F

C

C

E

E

T

S

S
x
N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

L

S

H

L

E

L

M

G

G

K

L

S

I

A

D

P

T

D

G

V

I

R

R

L

L

P

P

L

L

V

T

P

P

A

L

S

A

E

E

N

Q

A

Y

R

R

H

E

R

P

V

L

E

R

A

N

A

A

M

L

K

K

A

D

A

A

G

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding pyruvic acid: Y133 (= Y132), R138 (= R137), K161 (= K160), G186 (= G185), F244 (= F243), N248 (≠ S247)

3pueB Crystal structure of the complex of dhydrodipicolinate synthase from acinetobacter baumannii with lysine at 2.6a resolution
47% identity, 100% coverage: 2:290/290 of query aligns to 2:291/291 of 3pueB

query
sites
3pueB

F

I

K

Q

G

G

S

S

I

I

T

V

A

A

L

I

I

V

T

T

P

P

-

M

F

L

R

K

N

D

G

G

K

G

V

V

D

D

E

W

K

K

A

S

F

L

Q

E

D

K

L

L

V

V

A

E

W

W

Q

H

I

I

A

E

E

Q

G

G

T

T

H

N

A

S

V

I

V

V

P

A

C

V

G

G

T
|
T

T

T

G

G

E

E

S

A

P

S

T

T

L

L

S

S

H

M

D

E

E

E

H

H

H

T

R

Q

V

V

V

I

E

K

L

E

C

I

L

I

E

R

V

V

A

A

R

N

G

K

K

R

V

I

P

P

V

I

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

S

T

T

D

R

E

E

A

A

V

I

A

E

L

L

T

T

Q

K

H

A

A

A

R

K

K

D

A

L

G

G

A

A

D

D

A

A

L

L

V

L

V

V

A

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

Y

E

K

A

A

I

I

A

A

K

E

S

A

V

V

D

E

I

L

P

P

I

L

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

S

T

V

G

I

V

D

D

I

L

S

S

V

N

E

D

T

T

F

A

V

V

R

R

L

L

S

A

A

E

L

I

P

P

N

N

I

I

A

V

G

G

I

I

K
|
K

D

D

A

A

T

T

A

G

D

D

L

V

A

P

R

R

P

G

L

K

R

A

I

L

R

I

A

D

A

A

L

L

D

N

E

G

R

K

L

M

C

A

Q

V

L

Y

S

S

G

G

E

D

D

D

A

E

T

T

A

A

I

W

A

E

F

L

N

M

A

L

Q

L

G

G

G

A

V

D

G

G

C

N

I
|
I

S

S

V

V

T

T

S

A

N

N

I

I

A

A

P

P

K

K

L

A

C

M

A

S

R

E

M

V

Q

C

N

A

A

V

W

A

A

I

A

A

G

K

D

D

L

E

A

Q

T

Q

C

A

N

K

E

T

L

L

N

N

K

N

I

K

L

I

M

A

P

N

L

L

H

H

D

N

A

I

L

L

F
|
F

C

C

E
|
E

T
x
S

S
x
N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

L

S

H

L

E

L

M

G

G

K

L

S

I

A

D

P

T

D

G

V

I

R

R

L

L

P

P

L

L

V

T

P

P

A

L

S

A

E

E

N

Q

A

Y

R

R

H

E

R

P

V

L

E

R

A

N

A

A

M

L

K

K

A

D

A

A

G

G

L

I

I

I

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding lysine: R138 (= R137), F244 (= F243), E246 (= E245), S247 (≠ T246), N248 (≠ S247)

2atsA Dihydrodipicolinate synthase co-crystallised with (s)-lysine
47% identity, 100% coverage: 1:290/290 of query aligns to 1:292/292 of 2atsA

query
sites
2atsA

M

M

F

F

K

T

G

G

S

S

I

I

T

V

A

A

L

I

I

V

T

T

P

P

F

M

-

D

R

E

N

K

G

G

K

N

V

V

D

C

E

R

K

A

A

S

F

L

Q

K

D

K

L

L

V

I

A

D

W

Y

Q

H

I

V

A

A

E

S

G

G

T

T

H

S

A

A

V

I

V

V

P

S

C

V

G

G

T
|
T

T

T

G

G

E

E

S
|
S

P
x
A

T

T

L
|
L

S

N

H
|
H

D

D

E

E

H
|
H

H

A

R

D

V

V

E

M

L

M

C

T

L

L

E

D

V

L

A

A

R

D

G

G

K

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N
|
N

S

A

T

T

D

A

E
|
E

A

A

V

I

A

S

L

L

T

T

Q

Q

H

R

A

F

R

N

K

D

A

S

G

G

A

I

D

V

A

G

A

C

L

L

V

T

V

V

A

T

P

P

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

F

E

K

A

A

I

I

A

A

K

E

S

H

V

T

D

D

I

L

P

P

I

Q

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

S

T

V

G

I

C

D

D

I

L

S

L

V

P

E

E

T

T

F

V

V

G

R

R

L

L

S

A

A

K

L

V

P

K

N

N

I

I

A

I

G

G

I

I

K
|
K

D

E

A

A

T

T

A

G

D

N

L

L

A

T

R

R

P

V

L

N

R

Q

I

I

R

K

A

E

A

L

L

V

D

S

E

D

R

D

L

F

C

V

Q

L

L

L

S

S

G

G

E

D

D

D

A

A

T

S

A

A

I

L

A

D

F

F

N

M

A

Q

Q

L

G

G

V

H

G

G

C

V

I
|
I

S

S

V

V

T

T

S

A

N

N

I

V

A

A

P

A

K

R

L

D

C

M

A

A

R

Q

M

M

Q

C

N

K

A

L

W

A

A

A

A

E

G

G

D

H

L

F

A

A

T

E

C

A

N

R

E

V

L

I

N

N

K

Q

I

R

L

L

M

M

P

P

L

L

H

H

D

N

A

K

L

L

F

F

C

V

E

E

T

P

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

C

S

K

L

E

L

L

G

G

K

L

S

V

A

A

P

T

D

D

-

T

V

L

R

R

L

L

P

P

L

M

V

T

P

P

A

I

S

T

E

D

N

S

A

G

R

R

H

E

R

T

V

V

E

R

A

A

M

L

K

K

A

H

A

A

G

G

L

L

I

L

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), K161 (= K160), I203 (= I202)
binding d-lysine: S48 (= S47), A49 (≠ P48), L51 (= L50), H53 (= H52), H56 (= H55), N80 (= N79), E84 (= E83), Y106 (= Y105)

P0A6L2 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Escherichia coli (strain K12) (see 7 papers)
47% identity, 100% coverage: 1:290/290 of query aligns to 1:292/292 of P0A6L2

query
sites
P0A6L2

M

M

F

F

K

T

G

G

S

S

I

I

T

V

A

A

L

I

I

V

T

T

P

P

F

M

-

D

R

E

N

K

G

G

K

N

V

V

D

C

E

R

K

A

A

S

F

L

Q

K

D

K

L

L

V

I

A

D

W

Y

Q

H

I

V

A

A

E

S

G

G

T

T

H

S

A

A

V

I

V

V

P

S

C

V

G

G

T
|
T

G

G

E

E

S

S

P

A

T

T

L

L

S

N

H

H

D

D

E

E

H

H

H

A

R

D

V

V

E

M

L

M

C

T

L

L

E

D

V

L

A

A

R

D

G

G

K

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

A

T

T

D

A

E

E

A

A

V

I

A

S

L

L

T

T

Q

Q

H

R

A

F

R

N

K

D

A

S

G

G

A

I

D

V

A

G

A

C

L

L

V

T

V

V

A

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

F

E

K

A

A

I

I

A

A

K

E

S

H

V

T

D

D

I

L

P

P

I

Q

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

S

T

V

G

I

C

D

D

I

L

S

L

V

P

E

E

T

T

F

V

V

G

R

R

L

L

S

A

A

K

L

V

P

K

N

N

I

I

A

I

G

G

I

I

K
|
K

D

E

A

A

T

T

A

G

D

N

L

L

A

T

R

R

P

V

L

N

R

Q

I

I

R

K

A

E

A

L

L

V

D

S

E

D

R

D

L

F

C

V

Q

L

L

L

S

S

G

G

E

D

D

D

A

A

T

S

A

A

I

L

A

D

F

F

N

M

A

Q

Q
x
L

G

G

V

H

G

G

C

V

I
|
I

S

S

V

V

T

T

S

A

N

N

I

V

A

A

P

A

K

R

L

D

C

M

A

A

R

Q

M

M

Q

C

N

K

A

L

W

A

A

A

A

E

G

G

D

H

L

F

A

A

T

E

C

A

N

R

E

V

L

I

N

N

K

Q

I

R

L

L

M

M

P

P

L

L

H

H

D

N

A

K

L

L

F

F

C

V

E

E

T

P

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

C

S

K

L

E

L

L

G

G

K

L

S

V

A

A

P

T

D

D

-

T

V

L

R

R

L

L

P

P

L

M

V

T

P

P

A

I

S

T

E

D

N

S

A

G

R

R

H

E

R

T

V

V

E

R

A

A

M

L

K

K

A

H

A

A

G

G

L

L

I

L

T44 (= T43) mutation to S: 8% of wild-type activity. 4-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.; mutation to V: Reduced kcat by 99.9%.
T45 (= T44) binding
Y107 (= Y106) mutation to F: Reduced kcat by 90%.; mutation to W: Reduced activity by 95%. Reduced affinity for both substrates. Exists as a mixture of monomer, dimer and tetramer in solution. Has significantly lower thermal stability than the wild-type enzyme.
Y133 (= Y132) mutation to F: Reduced kcat by 99.7%. Reduced affinity for both substrates.
R138 (= R137) mutation R->A,H: Strongly increased KM for L-aspartate 4-semialdehyde. No effect on KM for pyruvate. Reduced activity by 99.7%.
K161 (= K160) mutation to A: 0.1% of wild-type activity. 3-fold decrease in affinity for pyruvate, and 2-fold decrease in that for (S)-ASA.; mutation to R: 0.35% of wild-type activity. 3-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.
L197 (≠ Q196) mutation L->Y,D: 1.4 to 2.5% of wild-type activity. Decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA. Exists as a dimer in solution.
I203 (= I202) binding

5t25A Kinetic, spectral and structural characterization of the slow binding inhibitor acetopyruvate with dihydrodipicolinate synthase from escherichia coli.
47% identity, 100% coverage: 1:290/290 of query aligns to 2:293/293 of 5t25A

query
sites
5t25A

M

M

F

F

K

T

G

G

S

S

I

I

T

V

A

A

L

I

I

V

T

T

P

P

F

M

-

D

R

E

N

K

G

G

K

N

V

V

D

C

E

R

K

A

A

S

F

L

Q

K

D

K

L

L

V

I

A

D

W

Y

Q

H

I

V

A

A

E

S

G

G

T

T

H

S

A

A

V

I

V

V

P

S

C

V

G

G

T
|
T

T

T

G

G

E

E

S
|
S

P
x
A

T

T

L
|
L

S

N

H
|
H

D

D

E

E

H
|
H

H

A

R

D

V

V

E

M

L

M

C

T

L

L

E

D

V

L

A

A

R

D

G

G

K

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N
|
N

S

A

T

T

D

A

E
|
E

A

A

V

I

A

S

L

L

T

T

Q

Q

H

R

A

F

R

N

K

D

A

S

G

G

A

I

D

V

A

G

A

C

L

L

V

T

V

V

A

T

P

P

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

F

E

K

A

A

I

I

A

A

K

E

S

H

V

T

D

D

I

L

P

P

I

Q

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

S

T

V

G

I

C

D

D

I

L

S

L

V

P

E

E

T

T

F

V

V

G

R

R

L

L

S

A

A

K

L

V

P

K

N

N

I

I

A

I

G

G

I

I

K
|
K

D

E

A

A

T

T

A

G

D

N

L

L

A

T

R

R

P

V

L

N

R

Q

I

I

R

K

A

E

A

L

L

V

D

S

E

D

R

D

L

F

C

V

Q

L

L

L

S

S

G

G

E

D

D

D

A

A

T

S

A

A

I

L

A

D

F

F

N

M

A

Q

Q

L

G

G

V

H

G

G

C

V

I
|
I

S

S

V

V

T

T

S

A

N

N

I

V

A

A

P

A

K

R

L

D

C

M

A

A

R

Q

M

M

Q

C

N

K

A

L

W

A

A

A

A

E

G

G

D

H

L

F

A

A

T

E

C

A

N

R

E

V

L

I

N

N

K

Q

I

R

L

L

M

M

P

P

L

L

H

H

D

N

A

K

L

L

F

F

C

V

E

E

T

P

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

C

S

K

L

E

L

L

G

G

K

L

S

V

A

A

P

T

D

D

-

T

V

L

R

R

L

L

P

P

L

M

V

T

P

P

A

I

S

T

E

D

N

S

A

G

R

R

H

E

R

T

V

V

E

R

A

A

M

L

K

K

A

H

A

A

G

G

L

L

I

L

active site: T45 (= T43), Y108 (= Y106), Y134 (= Y132), R139 (= R137), K162 (= K160), I204 (= I202)
binding lysine: S49 (= S47), A50 (≠ P48), L52 (= L50), H54 (= H52), H57 (= H55), N81 (= N79), E85 (= E83), Y107 (= Y105)

3i7sA Dihydrodipicolinate synthase mutant - k161a - with the substrate pyruvate bound in the active site. (see paper)
47% identity, 100% coverage: 1:290/290 of query aligns to 1:292/292 of 3i7sA

query
sites
3i7sA

M

M

F

F

K

T

G

G

S

S

I

I

T

V

A
|
A

L

I

I

V

T

T

P

P

F

M

-

D

R

E

N

K

G

G

K

N

V

V

D

C

E

R

K

A

A

S

F

L

Q

K

D

K

L

L

V

I

A

D

W

Y

Q

H

I

V

A

A

E

S

G

G

T

T

H

S

A

A

V

I

V

V

P

S

C

V

G
|
G

T
|
T

G

G

E

E

S

S

P

A

T

T

L

L

S

N

H

H

D

D

E

E

H

H

H

A

R

D

V

V

E

M

L

M

C

T

L

L

E

D

V

L

A

A

R

D

G

G

K

R

V

I

P

P

V

V

I

I

A

A

G

G

T

T

G

G

S

A

N

N

S

A

T

T

D

A

E

E

A

A

V

I

A

S

L

L

T

T

Q

Q

H

R

A

F

R

N

K

D

A

S

G

G

A

I

D

V

A

G

A

C

L
|
L

V

T

V

V

A

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

L

L

F

Y

R

Q

H

H

F

F

E

K

A

A

I

I

A

A

K

E

S

H

V

T

D

D

I

L

P

P

I

Q

I

I

V

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

S

T

V

G

I

C

D

D

I

L

S

L

V

P

E

E

T

T

F

V

V

G

R

R

L

L

S

A

A

K

L

V

P

K

N

N

I

I

A

I

G

G

I

I

K
x
R

D

E

A

A

T

T

A

G

D

N

L

L

A

T

R

R

P

V

L

N

R

Q

I

I

R

K

A

E

A

L

L

V

D

S

E

D

R

D

L

F

C

V

Q

L

L

L

S

S

G

G

E

D

D

D

A

A

T

S

A

A

I

L

A

D

F

F

N

M

A

Q

Q

L

G

G

V

H

G

G

C

V

I
|
I

S

S

V

V

T

T

S

A

N

N

I

V

A

A

P

A

K

R

L

D

C

M

A

A

R

Q

M

M

Q

C

N

K

A

L

W

A

A

A

A

E

G

G

D

H

L

F

A

A

T

E

C

A

N

R

E

V

L

I

N

N

K

Q

I

R

L

L

M

M

P

P

L

L

H

H

D

N

A

K

L

L

F

F

C

V

E

E

T

P

S

N

P

P

A

I

P

P

V

V

K

K

Y

W

A

A

A

C

S

K

L

E

L

L

G

G

K

L

S

V

A

A

P

T

D

D

-

T

V

L

R

R

L

L

P

P

L

M

V

T

P

P

A

I

S

T

E

D

N

S

A

G

R

R

H

E

R

T

V

V

E

R

A

A

M

L

K

K

A

H

A

A

G

G

L

L

I

L

active site: T44 (= T43), Y107 (= Y106), Y133 (= Y132), R138 (= R137), R161 (≠ K160), I203 (= I202)
binding pyruvic acid: A8 (= A8), G43 (= G42), T44 (= T43), T45 (= T44), L101 (= L100), Y133 (= Y132)

Q9X1K9 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
42% identity, 100% coverage: 1:290/290 of query aligns to 1:294/294 of Q9X1K9

query
sites
Q9X1K9

M

M

F

F

K

R

G

G

S

V

I

G

T

T

A

A

L

I

I

V

T

T

P

P

F

F

R

K

N

N

G

G

K

E

V

L

D

D

E

L

K

E

A

S

F

Y

Q

E

D

R

L

L

V

V

A

R

W

Y

Q

Q

I

L

A

E

E

N

G

G

T

V

H

N

A

A

V

L

V

I

P

V

C

L