SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_043744857.1 NCBI__GCF_000009985.1:WP_043744857.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

6n2oC 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
23% identity, 96% coverage: 8:205/206 of query aligns to 7:185/572 of 6n2oC

query
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6n2oC

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binding succinyl-coenzyme a: G14 (= G15), G16 (= G17), I17 (≠ V18), I18 (≠ M19), S19 (≠ T20), F41 (≠ A42), A43 (≠ M44), E44 (≠ A45), I45 (≠ Q46), R128 (= R145), N131 (= N148), F157 (= F174), N168 (= N185)

Sites not aligning to the query:

6n2oA 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
23% identity, 96% coverage: 8:205/206 of query aligns to 7:185/572 of 6n2oA

query
sites
6n2oA

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binding coenzyme a: G14 (= G15), G16 (= G17), S19 (≠ T20), N131 (= N148), K156 (≠ R173)

Sites not aligning to the query:

6n2nA Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
23% identity, 96% coverage: 8:205/206 of query aligns to 7:185/572 of 6n2nA

query
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6n2nA

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Sites not aligning to the query:

binding thiamine diphosphate: 223, 224, 225, 252

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
26% identity, 68% coverage: 46:185/206 of query aligns to 456:598/1171 of Q2RMD6

query
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Q2RMD6

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K456 (≠ Q46) binding
N556 (= N148) binding
N598 (= N185) binding

Sites not aligning to the query:

424:428 binding
686 binding
689 binding
692 binding
696 binding
742 binding
745 binding
748 binding
752 binding
809 binding
812 binding
814 binding
837 binding ; binding
967 binding
967:969 binding
995 binding
995:1000 binding
997 binding
1075 binding

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
26% identity, 68% coverage: 46:185/206 of query aligns to 455:597/1165 of 6cipA

query
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6cipA

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binding iron/sulfur cluster: K455 (≠ Q46)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865, 965, 966, 967, 996, 998, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836, 998, 1074

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
26% identity, 68% coverage: 46:185/206 of query aligns to 455:597/1164 of 6cioA

query
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6cioA

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binding iron/sulfur cluster: K455 (≠ Q46)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836, 1074, 1075
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
26% identity, 68% coverage: 46:185/206 of query aligns to 455:597/1169 of 6ciqA

query
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6ciqA

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-

F

-

K

R

Y

L

P

P

D

A

D

D

P

M

V

K

A

R

Q

T

M

I

R

A

A

T

A

K

G

K

V

L

T

K

V

F

Y

Y

D

N

F

I

D

D

A

A

G

V

A

K

I

I

A

A

R

Q

E

E

L

I

G

G

D

L

L

G

R

S

L
x
R

I

I

N

N

T

V

I

I

M

M

L

Q

G

T

A

A

-

F

-

F

-

K

I

I

A

A

D

N

Y

V

L

I

P

P

F

V

P

D

A

E

A

A

-

I

-

K

E

Y

L

I

E

K

E

D

Q

S

I

I

V

V

G

K

R

T

F
x
Y

R

G

E

K

R

K

K

G

P

D

A

K

M

I

V

L

E

N

V

M

N

N

binding coenzyme a: R553 (≠ L146), Y586 (≠ F174)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding coenzyme a: 28, 423, 424, 427, 428, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
26% identity, 68% coverage: 46:185/206 of query aligns to 455:597/1169 of 6cinB

query
sites
6cinB

Q
x
K

R

S

G

G

G

G

V

V

V

T

T

I

S

S

H

H

V

L

R

R

F

F

G

G

K

K

R

Q

V

P

W

I

S

Q

P

S

V

A

I

Y

T

L

P

I

G

D

T

Q

A

A

D

D

I

L

L

I

V

A

A

C

F

H

E

N

V

P

A

S

E

Y

G

V

S

G

R

R

W

Y

A

N

D

L

M

L

-

E

-

G

L

I

R

K

P

P

G

G

G

G

V

I

A

F

M

L

V

L

N

N

T

S

I

T

R

W

L

S

V

A

P

E

P

E

V

M

V

D

S

S

A

-

G

-

L

-

F

-

K

R

Y

L

P

P

D

A

D

D

P

M

V

K

A

R

Q

T

M

I

R

A

A

T

A

K

G

K

V

L

T

K

V

F

Y

Y

D

N

F

I

D

D

A

A

G

V

A

K

I

I

A

A

R

Q

E

E

L

I

G

G

D

L

L

G

R

S

L

R

I

I

N

N

T

V

I

I

M

M

L

Q

G

T

A

A

-

F

-

F

-

K

I

I

A

A

D

N

Y

V

L

I

P

P

F

V

P

D

A

E

A

A

-

I

-

K

E

Y

L

I

E

K

E

D

Q

S

I

I

V

V

G

K

R

T

F

Y

R

G

E

K

R

K

K

G

P

D

A

K

M

I

V

L

E

N

V

M

N

N

binding iron/sulfur cluster: K455 (≠ Q46)

Sites not aligning to the query:

active site: 28, 61, 111, 999
binding magnesium ion: 966, 994, 996
binding iron/sulfur cluster: 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836, 998, 1074, 1075
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865, 965, 966, 967, 996, 997, 998, 999, 1000

Query Sequence

>WP_043744857.1 NCBI__GCF_000009985.1:WP_043744857.1
MTDVITNILVCGTGGQGVMTAAEILSQAAMARGLDCKKSEVAGMAQRGGVVTSHVRFGKR
VWSPVITPGTADILVAFEVAEGSRWADMLRPGGVAMVNTIRLVPPVVSAGLFKYPDDPVA
QMRAAGVTVYDFDAGAIARELGDLRLINTIMLGAIADYLPFPAAELEEQIVGRFRERKPA
MVEVNQKAFAAGRDAAGSAKAKTSAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory